Gene Symbol: rde-4
Description: hypothetical protein
Species: Caenorhabditis elegans

Top Publications

  1. Kim J, Gabel H, Kamath R, Tewari M, Pasquinelli A, Rual J, et al. Functional genomic analysis of RNA interference in C. elegans. Science. 2005;308:1164-7 pubmed
    ..Moreover, the physical interactions among these potential RNAi factors suggest links to other RNA-dependent gene regulatory pathways. ..
  2. Parker G, Eckert D, Bass B. RDE-4 preferentially binds long dsRNA and its dimerization is necessary for cleavage of dsRNA to siRNA. RNA. 2006;12:807-18 pubmed
    ..Using extracts from wild-type and rde-4 mutant C. elegans, we show that the C-terminal dimerization domain is required for the production of siRNA. Our findings suggest a model for RDE-4 function during the initiation of RNAi. ..
  3. Parker G, Maity T, Bass B. dsRNA binding properties of RDE-4 and TRBP reflect their distinct roles in RNAi. J Mol Biol. 2008;384:967-79 pubmed publisher
  4. Tabara H, Sarkissian M, Kelly W, Fleenor J, Grishok A, Timmons L, et al. The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell. 1999;99:123-32 pubmed
    ..We discuss implications for the mechanism of RNAi and the possibility that one natural function of RNAi is transposon silencing. ..
  5. Tabara H, Yigit E, Siomi H, Mello C. The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans. Cell. 2002;109:861-71 pubmed
    ..Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing. ..
  6. Welker N, Habig J, Bass B. Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes. RNA. 2007;13:1090-102 pubmed
    ..However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes. ..
  7. Parrish S, Fire A. Distinct roles for RDE-1 and RDE-4 during RNA interference in Caenorhabditis elegans. RNA. 2001;7:1397-402 pubmed
    ..These results support a model in which RDE-4 is involved before or during production of siRNAs, whereas RDE-1 acts after the siRNAs have been formed. ..
  8. Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H. In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. EMBO J. 2007;26:5007-19 pubmed
    ..An Argonaute protein, CSR-1, is responsible for the Slicer activity induced by secondary-type siRNAs. Secondary rather than primary siRNAs may play a major role in the destabilization of target transcripts during RNAi in C. elegans. ..
  9. Habig J, Aruscavage P, Bass B. In C. elegans, high levels of dsRNA allow RNAi in the absence of RDE-4. PLoS ONE. 2008;3:e4052 pubmed publisher
    ..We conclude that an RNAi response varies with different conditions, and this may allow an organism to tailor a response to specific environmental signals. ..

More Information


  1. Blanchard D, Parameswaran P, Lopez Molina J, Gent J, Saynuk J, Fire A. On the nature of in vivo requirements for rde-4 in RNAi and developmental pathways in C. elegans. RNA Biol. 2011;8:458-67 pubmed
    ..Propagation at high temperatures exposed a conditional requirement for RDE-4 for optimal growth and fertility, indicating at least under these conditions that RDE-4 can serve an essential role in C. elegans. ..
  2. Mansisidor A, Cecere G, Hoersch S, Jensen M, Kawli T, Kennedy L, et al. A conserved PHD finger protein and endogenous RNAi modulate insulin signaling in Caenorhabditis elegans. PLoS Genet. 2011;7:e1002299 pubmed publisher
  3. Chiliveri S, Deshmukh M. Structure of RDE-4 dsRBDs and mutational studies provide insights into dsRNA recognition in the Caenorhabditis elegans RNAi pathway. Biochem J. 2014;458:119-30 pubmed publisher
    ..elegans. Additionally, we postulate a structural basis for the minimal requirement of linker and dsRBD2 for RDE-4's association with dsRNA and Dcr-1. ..
  4. Fischer S, Pan Q, Breen P, Qi Y, Shi Z, Zhang C, et al. Multiple small RNA pathways regulate the silencing of repeated and foreign genes in C. elegans. Genes Dev. 2013;27:2678-95 pubmed publisher
    ..The conserved ARF-like small GTPase ARL-8 is required specifically for primary siRNA biogenesis or stability in the sperm-specific ALG-3/4 endogenous RNAi pathway. ..
  5. Raman P, Zaghab S, Traver E, Jose A. The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans. Nucleic Acids Res. 2017;45:8463-8473 pubmed publisher
    ..elegans. We speculate that similar control of dsRNA movement could modulate tissue-specific silencing by feeding RNAi in other invertebrates. ..
  6. Thivierge C, Makil N, Flamand M, Vasale J, Mello C, Wohlschlegel J, et al. Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi. Nat Struct Mol Biol. 2011;19:90-7 pubmed publisher
    ..Our results implicate tudor proteins in the recruitment of RdRP complexes to specific steps within DCR-1-dependent and DCR-1-independent endo-RNAi pathways. ..