Gene Symbol: mei-1
Description: Meiotic spindle formation protein mei-1
Alias: Meiotic spindle formation protein mei-1
Species: Caenorhabditis elegans

Top Publications

  1. Kamath R, Martinez Campos M, Zipperlen P, Fraser A, Ahringer J. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2001;2:RESEARCH0002 pubmed
    ..Thus, RNAi by feeding should make possible new experimental approaches for the use of genomic sequence information. ..
  2. Pintard L, Willis J, Willems A, Johnson J, Srayko M, Kurz T, et al. The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase. Nature. 2003;425:311-6 pubmed
    ..Our results suggest that BTB-containing proteins may generally function as substrate-specific adaptors in Cul3-based E3-ubiquitin ligases. ..
  3. Xu L, Wei Y, Reboul J, Vaglio P, Shin T, Vidal M, et al. BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3. Nature. 2003;425:316-21 pubmed
    ..elegans CUL-3. Biochemical studies using the BTB protein MEL-26 and its genetic target MEI-1 (refs 12, 13) indicate that BTB proteins merge the functional properties of Skp1 and F-box proteins into a single polypeptide. ..
  4. Clark Maguire S, Mains P. Localization of the mei-1 gene product of Caenorhaditis elegans, a meiotic-specific spindle component. J Cell Biol. 1994;126:199-209 pubmed
    ..The genes mei-2 and mel-26 are part of a regulatory network that confines mei-1 activity to meiosis. ..
  5. Srayko M, Buster D, Bazirgan O, McNally F, Mains P. MEI-1/MEI-2 katanin-like microtubule severing activity is required for Caenorhabditis elegans meiosis. Genes Dev. 2000;14:1072-84 pubmed
    ..These data lead us to conclude that MEI-1/MEI-2 microtubule-severing activity is required for meiotic spindle organization in C. elegans. ..
  6. Pintard L, Kurz T, Glaser S, Willis J, Peter M, Bowerman B. Neddylation and deneddylation of CUL-3 is required to target MEI-1/Katanin for degradation at the meiosis-to-mitosis transition in C. elegans. Curr Biol. 2003;13:911-21 pubmed
    ..We conclude that both neddylation and deneddylation of CUL-3 is required for MEI-1 degradation and propose that cycles of CUL-3 neddylation and deneddylation are necessary for its ligase activity in vivo. ..
  7. Johnson J, Lu C, Raharjo E, McNally K, McNally F, Mains P. Levels of the ubiquitin ligase substrate adaptor MEL-26 are inversely correlated with MEI-1/katanin microtubule-severing activity during both meiosis and mitosis. Dev Biol. 2009;330:349-57 pubmed publisher
    ..We also show that MEI-1 is the only essential target for MEL-26, and possibly for the E3 ubiquitin ligase CUL-3, but the upstream ubiquitin ligase activating enzyme RFL-1 has additional essential targets. ..
  8. Gomes J, Tavernier N, Richaudeau B, Formstecher E, Boulin T, Mains P, et al. Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation. J Cell Biol. 2013;202:431-9 pubmed publisher
  9. Han X, Gomes J, Birmingham C, Pintard L, Sugimoto A, Mains P. The role of protein phosphatase 4 in regulating microtubule severing in the Caenorhabditis elegans embryo. Genetics. 2009;181:933-43 pubmed publisher
    ..The redundant regulatory pathways likely contribute in different ways to the rapid and precise post-meiotic inactivation of MEI-1 microtubule-severing activity. ..

More Information


  1. Yang H, McNally K, McNally F. MEI-1/katanin is required for translocation of the meiosis I spindle to the oocyte cortex in C elegans. Dev Biol. 2003;260:245-59 pubmed
    ..These results indicate a direct role of microtubule severing in translocation of the meiotic spindle to the cortex. ..
  2. Cheng K, Klancer R, Singson A, Seydoux G. Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition. Cell. 2009;139:560-72 pubmed publisher
    ..Our findings link cell-cycle progression to MBK-2/DYRK activation and the oocyte-to-embryo transition. ..
  3. Zehr E, Szyk A, Piszczek G, Szczęsna E, Zuo X, Roll Mecak A. Katanin spiral and ring structures shed light on power stroke for microtubule severing. Nat Struct Mol Biol. 2017;24:717-725 pubmed publisher
    ..Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing. ..
  4. Pellettieri J, Reinke V, Kim S, Seydoux G. Coordinate activation of maternal protein degradation during the egg-to-embryo transition in C. elegans. Dev Cell. 2003;5:451-62 pubmed
    ..We propose that MBK-2 functions as a temporal regulator of protein stability, and that coordinate activation of maternal protein degradation is one of the mechanisms that drives the transition from symmetric egg to patterned embryo. ..
  5. Wilson K, Qadota H, Mains P, Benian G. UNC-89 (obscurin) binds to MEL-26, a BTB-domain protein, and affects the function of MEI-1 (katanin) in striated muscle of Caenorhabditis elegans. Mol Biol Cell. 2012;23:2623-34 pubmed publisher
    ..The level of MEI-1 protein is reduced in an unc-89 mutant, suggesting that the normal role of UNC-89 is to inhibit the CUL-3/MEL-26 complex toward MEI-1. ..
  6. McNally K, Berg E, Cortes D, Hernández V, Mains P, McNally F. Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro. Mol Biol Cell. 2014;25:1037-49 pubmed publisher
    ..These activities could promote parallel/antiparallel microtubule organization in meiotic spindles. ..
  7. Connolly A, Osterberg V, Christensen S, Price M, Lu C, Chicas Cruz K, et al. Caenorhabditis elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1. Mol Biol Cell. 2014;25:1298-311 pubmed publisher
    ..We conclude that microtubule severing and ASPM-1 both promote meiotic spindle pole assembly in C. elegans oocytes, whereas the kinesin 12 family member KLP-18 promotes spindle bipolarity. ..