Gene Symbol: let-99
Description: Protein let-99
Alias: Protein let-99
Species: Caenorhabditis elegans
Rose L, Kemphues K. The let-99 gene is required for proper spindle orientation during cleavage of the C. elegans embryo. Development. 1998;125:1337-46 pubmed
Tsou M, Hayashi A, DeBella L, McGrath G, Rose L. LET-99 determines spindle position and is asymmetrically enriched in response to PAR polarity cues in C. elegans embryos. Development. 2002;129:4469-81 pubmed
..Together, the data indicate that LET-99 acts downstream of PAR-3 and PAR-2 to determine spindle positioning, potentially through the asymmetric regulation of forces on the spindle. ..
Tsou M, Hayashi A, Rose L. LET-99 opposes Galpha/GPR signaling to generate asymmetry for spindle positioning in response to PAR and MES-1/SRC-1 signaling. Development. 2003;130:5717-30 pubmed
..These results provide insight into how polarity cues are transmitted into specific spindle positions in both extrinsic and intrinsic pathways of asymmetric cell division. ..
Wu J, Rose L. PAR-3 and PAR-1 inhibit LET-99 localization to generate a cortical band important for spindle positioning in Caenorhabditis elegans embryos. Mol Biol Cell. 2007;18:4470-82 pubmed
..Finally, examination of par-1 embryos suggests that the banded pattern of LET-99 is critical for normal posterior spindle displacement and to prevent spindle misorientation caused by cell shape constraints. ..
Krueger L, Wu J, Tsou M, Rose L. LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos. J Cell Biol. 2010;189:481-95 pubmed publisher
..We propose that the attenuation of lateral forces prevents counterproductive pulling, resulting in a higher net force toward the posterior that contributes to spindle elongation and displacement. ..
Wu J, Espiritu E, Rose L. The 14-3-3 protein PAR-5 regulates the asymmetric localization of the LET-99 spindle positioning protein. Dev Biol. 2016;412:288-297 pubmed publisher
..Together the results indicate that PAR-5 acts in a mechanism with PAR-1 to regulate LET-99 cortical localization. ..
Sendoel A, Maida S, Zheng X, Teo Y, Stergiou L, Rossi C, et al
. DEPDC1/LET-99 participates in an evolutionarily conserved pathway for anti-tubulin drug-induced apoptosis. Nat Cell Biol. 2014;16:812-20 pubmed publisher
..Collectively, these data uncover an evolutionarily conserved mediator of anti-tubulin drug-induced apoptosis and suggest that DEPDC1 levels could be an additional determinant for therapy response upstream of MCL1. ..
Liro M, Rose L. Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA. Genetics. 2016;204:1177-1189 pubmed publisher
..We show that LET-99 acts in the Mes-1/Src-1 pathway for spindle positioning. LIN-5 is also required for EMS spindle positioning, possibly through a G?- and DLG-1-independent mechanism. ..
Price K, Rose L. LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring. Mol Biol Cell. 2017;28:2360-2373 pubmed publisher
..Once positioned there, LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells. ..