Gene Symbol: fbf-2
Description: Fem-3 mRNA-binding factor 2
Alias: Fem-3 mRNA-binding factor 2
Species: Caenorhabditis elegans
Products:     fbf-2

Top Publications

  1. Luitjens C, Gallegos M, Kraemer B, Kimble J, Wickens M. CPEB proteins control two key steps in spermatogenesis in C. elegans. Genes Dev. 2000;14:2596-609 pubmed
    ..In sum, our results demonstrate that, in C. elegans, two CPEB proteins have distinct functions in the germ line, both in spermatogenesis: FOG-1 specifies the sperm cell fate and CPB-1 executes that decision. ..
  2. Eckmann C, Crittenden S, Suh N, Kimble J. GLD-3 and control of the mitosis/meiosis decision in the germline of Caenorhabditis elegans. Genetics. 2004;168:147-60 pubmed
    ..Animals homozygous for gld-3(q741) enter meiosis, but are feminized. Therefore, GLD-3 promotes meiosis primarily via its interaction with GLD-2, and it promotes spermatogenesis primarily via its interaction with FBF. ..
  3. Lamont L, Crittenden S, Bernstein D, Wickens M, Kimble J. FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans germline. Dev Cell. 2004;7:697-707 pubmed
    ..Therefore, fbf-1 and fbf-2 provide a paradigm for how recently duplicated genes can diverge to fine-tune patterning during animal development. ..
  4. Thompson B, Bernstein D, Bachorik J, Petcherski A, Wickens M, Kimble J. Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB. Development. 2005;132:3471-81 pubmed
    ..The dose-dependent control of proliferation and cell fate by FOG-1 has striking parallels with Xenopus CPEB, suggesting a conserved mechanism in animal development. ..
  5. Walser C, Battu G, Hoier E, Hajnal A. Distinct roles of the Pumilio and FBF translational repressors during C. elegans vulval development. Development. 2006;133:3461-71 pubmed
    ..Thus, translational repressors regulate various aspects of vulval cell fate specification, and they may play a conserved role in modulating signal transduction during animal development. ..
  6. Zhang B, Gallegos M, Puoti A, Durkin E, Fields S, Kimble J, et al. A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line. Nature. 1997;390:477-84 pubmed
    ..We propose that FBF and Pumilio are members of a widespread family of sequence-specific RNA-binding proteins. ..
  7. Labbé J, Pacquelet A, Marty T, Gotta M. A genomewide screen for suppressors of par-2 uncovers potential regulators of PAR protein-dependent cell polarity in Caenorhabditis elegans. Genetics. 2006;174:285-95 pubmed
    ..Taken together, our results identify conserved components that regulate PAR protein function and also suggest a role for NOS-3 in PAR protein-dependent cell polarity. ..
  8. Kershner A, Kimble J. Genome-wide analysis of mRNA targets for Caenorhabditis elegans FBF, a conserved stem cell regulator. Proc Natl Acad Sci U S A. 2010;107:3936-41 pubmed publisher
    ..We speculate that shared PUF targets represent part of a broadly used module of stem cell control. ..
  9. Cooke A, Prigge A, Opperman L, Wickens M. Targeted translational regulation using the PUF protein family scaffold. Proc Natl Acad Sci U S A. 2011;108:15870-5 pubmed publisher
    ..The PUF-effector strategy enables the design of proteins that affect translation and stability of specific mRNAs in vivo. ..

More Information


  1. Wu J, Campbell Z, Menichelli E, Wickens M, Williamson J. A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex. J Mol Biol. 2013;425:738-54 pubmed publisher
    ..These data raise the possibility of alternative models involving the formation of a GLD-3?FBF?RNA complex in the regulation of germline mRNAs. ..
  2. Merritt C, Seydoux G. The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells. Development. 2010;137:1787-98 pubmed publisher
    ..We propose that parallel regulation by FBF ensures that in wild-type gonads, meiotic entry is coordinated with just-in-time synthesis of synaptonemal proteins...
  3. Menichelli E, Wu J, Campbell Z, Wickens M, Williamson J. Biochemical characterization of the Caenorhabditis elegans FBF.CPB-1 translational regulation complex identifies conserved protein interaction hotspots. J Mol Biol. 2013;425:725-37 pubmed publisher
    ..RNA binding measurements demonstrated that CPB-1 alters the affinity of FBF for specific RNA sequences, implying a functional model where the coregulatory protein CPB-1 modulates FBF target selection. ..
  4. Voronina E, Paix A, Seydoux G. The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells. Development. 2012;139:3732-40 pubmed
    ..Our findings also support the view that P granules facilitate mRNA silencing by providing an environment in which translational repressors can encounter their mRNA targets immediately upon exit from the nucleus. ..