ama-1

Summary

Gene Symbol: ama-1
Description: DNA-directed RNA polymerase II subunit RPB1
Alias: DNA-directed RNA polymerase II subunit RPB1
Species: Caenorhabditis elegans
Products:     ama-1

Top Publications

  1. Sanford T, Golomb M, Riddle D. RNA polymerase II from wild type and alpha-amanitin-resistant strains of Caenorhabditis elegans. J Biol Chem. 1983;258:12804-9 pubmed
    ..Thus, ama-1 appears to affect a subunit of RNA polymerase II. ..
  2. Kuroyanagi H, Kimura T, Wada K, Hisamoto N, Matsumoto K, Hagiwara M. SPK-1, a C. elegans SR protein kinase homologue, is essential for embryogenesis and required for germline development. Mech Dev. 2000;99:51-64 pubmed
    ..elegans. We provide evidence that RNAi, achieved by the soaking of L1 larvae, is beneficial in the study of gene function in post-embryonic germline development. ..
  3. Kwon J, Park J, Gim B, Han S, Lee J, Kim Y. Caenorhabditis elegans mediator complexes are required for developmental-specific transcriptional activation. Proc Natl Acad Sci U S A. 1999;96:14990-5 pubmed
    ..Therefore, the gene-specific function of Mediator as an integrator of transcriptional regulatory signals is evolutionarily conserved and is essential for C. elegans development. ..
  4. Wallenfang M, Seydoux G. cdk-7 Is required for mRNA transcription and cell cycle progression in Caenorhabditis elegans embryos. Proc Natl Acad Sci U S A. 2002;99:5527-32 pubmed
    ..Our results support a dual role for metazoan CDK7 as a broadly required CTD kinase, and as a CAK essential for cell cycle progression. ..
  5. Walker A, Shi Y, Blackwell T. An extensive requirement for transcription factor IID-specific TAF-1 in Caenorhabditis elegans embryonic transcription. J Biol Chem. 2004;279:15339-47 pubmed
    ..Our findings imply that in metazoans TFIID may be of widespread importance for transcription and for expression of tissue-specific genes. ..
  6. Bowman E, Riddle D, Kelly W. Amino Acid Substitutions in the Caenorhabditis elegans RNA Polymerase II Large Subunit AMA-1/RPB-1 that Result in ?-Amanitin Resistance and/or Reduced Function. G3 (Bethesda). 2011;1:411-6 pubmed publisher
    ..We identified 12 of these mutations and mapped them onto the Saccharomyces cerevisiae RPB1 structure to provide insight into AMA-1 regions that are essential for development in a multicellular organism. ..
  7. Eberhard R, Stergiou L, Hofmann E, Hofmann J, Haenni S, Teo Y, et al. Ribosome synthesis and MAPK activity modulate ionizing radiation-induced germ cell apoptosis in Caenorhabditis elegans. PLoS Genet. 2013;9:e1003943 pubmed publisher
    ..Ribosome synthesis and growth-factor signalling are perturbed in many cancer cells; such an interplay between basic cellular processes and signalling might be critical for how tumours evolve or respond to treatment. ..
  8. Ahn J, Rechsteiner A, Strome S, Kelly W. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. 2016;12:e1006227 pubmed publisher
    ..Our findings suggest that SIG-7 plays a central role in both Pol II elongation and co-transcriptional splicing and may provide an important link for their coordination and regulation. ..
  9. Bird D, Riddle D. Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II. Mol Cell Biol. 1989;9:4119-30 pubmed
    ..By contrast with ama-1, rpc-1 was not deleted by mDf4 or larger deficiencies examined, indicating that these genes are no closer than 150 kb. Genes flanking ama-1, including two collagen genes, also have been identified. ..

More Information

Publications16

  1. Seydoux G, Fire A. Soma-germline asymmetry in the distributions of embryonic RNAs in Caenorhabditis elegans. Development. 1994;120:2823-34 pubmed
    ..These observations suggest that mechanisms which distinguish between soma and germline cause asymmetries in mRNA stability and transcription within the first few cleavages of C. elegans embryogenesis. ..
  2. Powell Coffman J, Knight J, Wood W. Onset of C. elegans gastrulation is blocked by inhibition of embryonic transcription with an RNA polymerase antisense RNA. Dev Biol. 1996;178:472-83 pubmed
    ..These results indicate that embryonically transcribed gene products are required for gastrulation initiation. They also demonstrate the efficacy of a method for blocking embryonic transcription that may be useful in other organisms. ..
  3. Tabara H, Hill R, Mello C, Priess J, Kohara Y. pos-1 encodes a cytoplasmic zinc-finger protein essential for germline specification in C. elegans. Development. 1999;126:1-11 pubmed
  4. Rogalski T, Golomb M, Riddle D. Mutant Caenorhabditis elegans RNA polymerase II with a 20,000-fold reduced sensitivity to alpha-amanitin. Genetics. 1990;126:889-98 pubmed
    ..The highly resistant double mutant was selected among four million progeny of the mutagenized ama-1(m118) parent by its ability to grow and reproduce in 200 micrograms/ml amanitin in the presence of a permeabilizing agent, Triton X-100. ..
  5. Rogalski T, Riddle D. A Caenorhabditis elegans RNA polymerase II gene, ama-1 IV, and nearby essential genes. Genetics. 1988;118:61-74 pubmed
    ..1 map unit. The terminal phenotype of mDf10 homozygotes is developmental arrest during the first larval stage, suggesting that there is sufficient maternal RNA polymerase II to complete embryonic development. ..
  6. Walston T, Tuskey C, Edgar L, Hawkins N, Ellis G, Bowerman B, et al. Multiple Wnt signaling pathways converge to orient the mitotic spindle in early C. elegans embryos. Dev Cell. 2004;7:831-41 pubmed
  7. Bender L, Suh J, Carroll C, Fong Y, Fingerman I, Briggs S, et al. MES-4: an autosome-associated histone methyltransferase that participates in silencing the X chromosomes in the C. elegans germ line. Development. 2006;133:3907-17 pubmed
    ..We discuss how an autosomally associated HMT may participate in silencing genes on the X chromosome, in coordination with the direct silencing effects of the other MES proteins. ..