Gene Symbol: recA
Description: recombinase RecA
Species: Burkholderia xenovorans LB400
Nakazawa T, Kimoto M, Abe M. Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepacia. Gene. 1990;94:83-8 pubmed
A recombinant plasmid carrying the recA gene of Pseudomonas cepacia complements a recA mutation of Escherichia coli and restores UV and methylmethane sulfonate resistance, as well as recombinational proficiency...
van Waasbergen L, Kidambi S, Miller R. Construction of a recA mutant of Burkholderia (formerly Pseudomonas), cepacia. Appl Microbiol Biotechnol. 1998;49:59-65 pubmed
A recA mutant was constructed of a soil isolate of Burkholderia cepacia, strain ATCC 17616...
Coenye T, Mahenthiralingam E, Henry D, LiPuma J, Laevens S, Gillis M, et al
. Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol. 2001;51:1481-90 pubmed publisher
..ornithine and lysine decarboxylase activity, acidification of sucrose and beta-haemolysis) and a newly developed recA gene-based PCR assay. 16S rDNA-based RFLP analysis and PCR tests allowed differentiation of B...
Yeager C, Bottomley P, Arp D. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Appl Environ Microbiol. 2001;67:5384-91 pubmed publisher
..sites were mapped within open reading frames putatively encoding enzymes involved in DNA repair (UvrB, RuvB, RecA, and RecG) in 7 of the 11 TCS strains obtained (4 of the TCS strains had a single Tn5 insertion within a uvrB ..
Chan C, Stead D, Coutts R. Development of a species-specific recA-based PCR test for Burkholderia fungorum. FEMS Microbiol Lett. 2003;224:133-8 pubmed
The genus Burkholderia comprises over 28 species and species-specific, recA-based polymerase chain reaction (PCR) tests are available for several species, but not for some soil-inhabiting species including B. fungorum...
Detsika M, Corkill J, Magalhaes M, Glendinning K, Hart C, Winstanley C. Molecular typing of, and distribution of genetic markers among, Burkholderia cepacia complex isolates from Brazil. J Clin Microbiol. 2003;41:4148-53 pubmed
..2%), B. vietnamiensis (18.2%), and genomovar I (9.1%). Six CF isolates sharing recA PCR-restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD) patterns could ..
Storms V, Van Den Vreken N, Coenye T, Mahenthiralingam E, LiPuma J, Gillis M, et al
. Polyphasic characterisation of Burkholderia cepacia-like isolates leading to the emended description of Burkholderia pyrrocinia. Syst Appl Microbiol. 2004;27:517-26 pubmed
Twenty-five Burkholderia cepacia-like isolates of human and environmental origin, comprising five different recA RFLP types, were examined by using a polyphasic taxonomic approach, including recA gene sequence analysis, 16S rRNA gene ..
Ramette A, LiPuma J, Tiedje J. Species abundance and diversity of Burkholderia cepacia complex in the environment. Appl Environ Microbiol. 2005;71:1193-201 pubmed publisher
..In addition, a duplex PCR targeting B. cepacia complex-specific recA and 16S rRNA sequences was developed to enable a fast and reliable diagnostic assay for members of the complex...
Vandamme P, Henry D, Coenye T, Nzula S, Vancanneyt M, LiPuma J, et al
. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunol Med Microbiol. 2002;33:143-9 pubmed
..cepacia complex. This highlights another potential source for diagnostic problems with B. cepacia-like bacteria...