ospA

Summary

Gene Symbol: ospA
Description: outer surface protein A (OspA)
Species: Borrelia burgdorferi B31

Top Publications

  1. ncbi Recombinant outer surface protein a from Borrelia burgdorferi induces antibodies protective against spirochetal infection in mice
    M M Simon
    Max Planck Institut fur Immunbiologie, Freiburg, Germany
    J Infect Dis 164:123-32. 1991
  2. pmc Cloning and sequencing of the gene encoding the outer surface protein A (OspA) of a European Borrelia burgdorferi isolate
    R Wallich
    Department of Applied Immunology, German Cancer Research Center, DKFZ, Heidelberg, FRG
    Nucleic Acids Res 17:8864. 1989
  3. pmc Abilities of OspA proteins from different seroprotective groups of Borrelia burgdorferi to protect hamsters from infection
    S D Lovrich
    Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison, USA
    Infect Immun 63:2113-9. 1995
  4. pmc OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier
    Lucia Pulzova
    Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, 04181, Kosice, Slovakia
    Sci Rep 1:86. 2011
  5. pmc Probing the Borrelia burgdorferi surface lipoprotein secretion pathway using a conditionally folding protein domain
    Shiyong Chen
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6724-32. 2011
  6. pmc Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering
    Koki Makabe
    Department of Biochemistry and Molecular Biology, The University of Chicago, Illinois 60637, USA
    Protein Sci 15:1907-14. 2006
  7. pmc Hydrophobic surface burial is the major stability determinant of a flat, single-layer beta-sheet
    Shude Yan
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
    J Mol Biol 368:230-43. 2007
  8. pmc Borrelia burgdorferi intercepts host hormonal signals to regulate expression of outer surface protein A
    Mark R Scheckelhoff
    Division of Geographic Medicine and Infectious Diseases, Tupper Research Institute, Tufts New England Medical Center, 750 Washington Street, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 104:7247-52. 2007
  9. pmc Human homologues of a Borrelia T cell epitope associated with antibiotic-refractory Lyme arthritis
    Elise E Drouin
    Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Mol Immunol 45:180-9. 2008
  10. pmc Beta-strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of beta-strand pairing
    Koki Makabe
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA
    J Am Chem Soc 129:14661-9. 2007

Scientific Experts

  • Paul H Duray
  • Elise E Drouin
  • Rodolfo C Garcia
  • Pauliina Hartiala
  • Jan Sterba
  • Shiyong Chen
  • Koki Makabe
  • Qilong Xu
  • Shude Yan
  • Wolfram R Zückert
  • Shohei Koide
  • Valentina Tereshko
  • Grzegorz Gawlak
  • Lucia Pulzova
  • Ozan S Kumru
  • Ryan J Schulze
  • Kristy McShan
  • James M Battisti
  • Fang Ting Liang
  • Mark R Scheckelhoff
  • Utpal Pal
  • Marian Madar
  • Rastislav Mucha
  • Andrej Kovac
  • Patrik Mlynarcik
  • Elena Bencúrová
  • Mangesh Bhide
  • Michal Novak
  • B J Luft
  • W Ding
  • J J Dunn
  • Patricia A Rosa
  • Paul F Policastro
  • James L Bono
  • Merry E Schrumpf
  • Tom G Schwan
  • Akiko Koide
  • C L Lawson
  • Linden T Hu
  • Mary Wesley
  • Sam R Telford
  • M Sole
  • Nandhini Ramamoorthi
  • Xiaofeng Yang
  • Fred S Kantor
  • Sam Telford
  • Tian Wang
  • Aravinda M Desilva
  • H Li
  • Marc Pypaert
  • Fukai Bao
  • Xin Li
  • Erol Fikrig
  • Ruth R Montgomery
  • John F Anderson
  • Deepti Pradhan
  • X Huang
  • S Koide
  • G Will
  • X Yang
  • S D Lovrich
  • C Bantar
  • K Indest
  • D A Caporale
  • M T Philipp
  • B Wilske
  • Y Gu
  • R Ramamoorthy
  • R Coughlin
  • V Preac-Mursic
  • D E Dykhuizen
  • P A Rosa
  • U Busch
  • S M Callister
  • S P Day
  • B K DuChateau
  • E Schwab
  • J Winfrey
  • S Jauris-Heipke
  • L C Lim
  • E Soutschek
  • D Rossler
  • R F Schell
  • E Fikrig
  • A Sadziene
  • T D Kocher
  • R J Dattwyler
  • R Wallich
  • M M Simon
  • D S Polin

Detail Information

Publications30

  1. ncbi Recombinant outer surface protein a from Borrelia burgdorferi induces antibodies protective against spirochetal infection in mice
    M M Simon
    Max Planck Institut fur Immunbiologie, Freiburg, Germany
    J Infect Dis 164:123-32. 1991
    The outer surface protein A (OspA) of Borrelia burgdorferi was isolated in its native form from strains ZS7 and B31 and as a recombinant protein from strain ZS7...
  2. pmc Cloning and sequencing of the gene encoding the outer surface protein A (OspA) of a European Borrelia burgdorferi isolate
    R Wallich
    Department of Applied Immunology, German Cancer Research Center, DKFZ, Heidelberg, FRG
    Nucleic Acids Res 17:8864. 1989
  3. pmc Abilities of OspA proteins from different seroprotective groups of Borrelia burgdorferi to protect hamsters from infection
    S D Lovrich
    Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison, USA
    Infect Immun 63:2113-9. 1995
    The ability of vaccination with recombinant OspA from six seroprotective groups of Borrelia burgdorferi sensu lato to induce protection against infection with homologous and other Lyme spirochetes was examined in hamsters...
  4. pmc OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier
    Lucia Pulzova
    Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, 04181, Kosice, Slovakia
    Sci Rep 1:86. 2011
    ..Using protein-protein interaction assays we found that CD40 of BMECs and OspA of Borrelia are the primary molecules in transient tethering of Borrelia to endothelium...
  5. pmc Probing the Borrelia burgdorferi surface lipoprotein secretion pathway using a conditionally folding protein domain
    Shiyong Chen
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6724-32. 2011
    ..bacterial outer membrane (OM), we generated constructs in which Borrelia burgdorferi outer surface lipoprotein A (OspA) was fused to calmodulin (CaM), a conserved eukaryotic protein undergoing calcium-dependent folding...
  6. pmc Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering
    Koki Makabe
    Department of Biochemistry and Molecular Biology, The University of Chicago, Illinois 60637, USA
    Protein Sci 15:1907-14. 2006
    Outer surface protein A (OspA) from Borrelia burgdorferi has an unusual dumbbell-shaped structure in which two globular domains are connected with a "single-layer" beta-sheet (SLB)...
  7. pmc Hydrophobic surface burial is the major stability determinant of a flat, single-layer beta-sheet
    Shude Yan
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
    J Mol Biol 368:230-43. 2007
    ..mutagenesis experiments on the single-layer beta-sheet segment of Borrelia outer surface protein A (OspA)...
  8. pmc Borrelia burgdorferi intercepts host hormonal signals to regulate expression of outer surface protein A
    Mark R Scheckelhoff
    Division of Geographic Medicine and Infectious Diseases, Tupper Research Institute, Tufts New England Medical Center, 750 Washington Street, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 104:7247-52. 2007
    ..Outer surface protein A (OspA) is a borrelial protein important in colonization of the tick midgut...
  9. pmc Human homologues of a Borrelia T cell epitope associated with antibiotic-refractory Lyme arthritis
    Elise E Drouin
    Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Mol Immunol 45:180-9. 2008
    ..is associated with HLA-DR molecules that bind an epitope of Borrelia burgdorferi (Bb) outer-surface protein A (OspA(165-173)) and with T cell reactivity with this epitope...
  10. pmc Beta-strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of beta-strand pairing
    Koki Makabe
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA
    J Am Chem Soc 129:14661-9. 2007
    ..adjacent beta-strands is specified using a series of mutants of the single-layer beta-sheet (SLB) in Borrelia OspA. The single-layer architecture of this system eliminates structural restraints imposed by a hydrophobic core, ..
  11. ncbi Borrelia burgdorferi inhibits human neutrophil functions
    Pauliina Hartiala
    Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, 20520 Turku, Finland
    Microbes Infect 10:60-8. 2008
    Outer surface proteins OspA and OspB are among the most prominent Borrelia burgdorferi surface molecules. We constructed OspAB and OspA complementation mutants of B...
  12. pmc Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated
    Jan Sterba
    Biology Center of Academy of Sciences of the Czech Republic, Institute of Parasitology, Branisovska 31, CZ 37005 Ceske Budejovice, Czech Republic
    J Bacteriol 190:2619-23. 2008
    ..We did not find any evidence for glycosylation of the major outer membrane proteins OspA and OspB or the structural flagellar proteins FlaB and FlaA...
  13. pmc Essential protective role attributed to the surface lipoproteins of Borrelia burgdorferi against innate defences
    Qilong Xu
    Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Mol Microbiol 69:15-29. 2008
    ..Increasing expression of any of the four randomly chosen surface lipoproteins, OspA, OspE, VlsE or DbpA, fully protected the ospC mutant from elimination from the skin tissue of SCID mice; moreover, ..
  14. pmc Outer surface protein A protects Lyme disease spirochetes from acquired host immunity in the tick vector
    James M Battisti
    Rocky Mountain Laboratories, NIAID, NIH, 903 S 4th Street, Hamilton, MT 59840, USA
    Infect Immun 76:5228-37. 2008
    ..b>OspA is produced as borreliae enter the tick vector and remains a major surface antigen during midgut colonization...
  15. doi Modification of Borrelia burgdorferi to overproduce OspA or VlsE alters its infectious behaviour
    Qilong Xu
    Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Microbiology 154:3420-9. 2008
    ..burgdorferi was modified to constitutively produce the well-characterized surface lipoproteins OspA and invariant VlsE...
  16. pmc Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus
    Ryan J Schulze
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Microbiol 76:1266-78. 2010
    ..Here, we show that individual deletion of four contiguous residues within the tether of major surface lipoprotein OspA results in its inefficient translocation across the Borrelia outer membrane...
  17. pmc Determination of Borrelia surface lipoprotein anchor topology by surface proteolysis
    Shiyong Chen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6379-83. 2011
    ..assay to probe accessibility of the membrane-proximal N-terminal tether peptides of Borrelia surface lipoproteins OspA and Vsp1...
  18. pmc Complement receptor 3 binds the Borrelia burgdorferi outer surface proteins OspA and OspB in an iC3b-independent manner
    Rodolfo C Garcia
    Leukocyte Biology Unit, I C G E B, Area Science Park, 34012 Trieste, Italy
    Infect Immun 73:6138-42. 2005
    ..The present work demonstrates the direct binding of the Borrelia burgdorferi outer surface proteins OspA and OspB to CR3 and that this binding is independent of iC3b.
  19. ncbi Invasion of human tissue ex vivo by Borrelia burgdorferi
    Paul H Duray
    Department of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 191:1747-54. 2005
    ..Also, we evaluated the expression of the outer surface proteins (Osps) OspA and OspC by use of quantitative Western blotting...
  20. ncbi Recombination between genes encoding major outer surface proteins A and B of Borrelia burgdorferi
    P A Rosa
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840
    Mol Microbiol 6:3031-40. 1992
    ..burgdorferi in an infected host, and has important implications for the utility of OspA and OspB as diagnostic or vaccine candidates for Lyme disease...
  21. ncbi Borrelia burgdorferi strain 25015: characterization of outer surface protein A and vaccination against infection
    E Fikrig
    Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06510
    J Immunol 148:2256-60. 1992
    Mice vaccinated with outer surface protein A (OspA) from Borrelia burgdorferi strain N40 are protected from challenge with an intradermal syringe inoculum of B. burgdorferi strains N40, B31, and CD16...
  22. ncbi Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi
    S Bergstrom
    Department of Microbiology, University of Umeea, Sweden
    Mol Microbiol 3:479-86. 1989
    The ospA and ospB genes encode the major outer membrane proteins of the Lyme disease spirochaete Borrelia burgdorferi...
  23. ncbi Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
    G Will
    Abteilung Serologie, Max von Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie der Ludwig Maximilians Universität München, Germany
    Med Microbiol Immunol 184:73-80. 1995
    The genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1-7, previously described [Wilske et al...
  24. ncbi Sequence variation in the outer-surface-protein genes of Borrelia burgdorferi
    D A Caporale
    Department of Zoology, University of New Hampshire, Durham 03824
    Mol Biol Evol 11:51-64. 1994
    ..We sequenced the OspA and OspB genes of nine North American strains and compared them with nine strains previously described...
  25. pmc Borrelia burgdorferi is clonal: implications for taxonomy and vaccine development
    D E Dykhuizen
    Department of Ecology and Evolution, State University of New York, Stony Brook 11794
    Proc Natl Acad Sci U S A 90:10163-7. 1993
    The chromosomal genes fla and p93 and the ospA gene from a linear plasmid were sequenced from up to 15 isolates of Borrelia burgdorferi, which causes Lyme borreliosis in man...
  26. pmc Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab
    H Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Proc Natl Acad Sci U S A 94:3584-9. 1997
    b>OspA (outer surface protein A) is an abundant immunogenic lipoprotein of the Lyme disease spirochete Borrelia burgdorferi...
  27. pmc Borrelia burgdorferi escape mutants that survive in the presence of antiserum to the OspA vaccine are killed when complement is also present
    M Sole
    Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, USA
    Infect Immun 66:2540-6. 1998
    As an initial attempt to investigate the possible role of outer surface protein A (OspA) escape mutants of Borrelia burgdorferi in decreasing the efficacy of the OspA vaccine, mutants of the HB19 strain of B...
  28. ncbi Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA
    W Ding
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Mol Biol 302:1153-64. 2000
    Outer surface protein A (OspA) is a major lipoprotein of the Borrelia burgdorferi spirochete, the causative agent of Lyme disease...
  29. ncbi TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 119:457-68. 2004
    ..Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature...
  30. pmc Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization
    A Sadziene
    Department of Microbiology, University of Texas Health Science Center, San Antonio 78284
    J Exp Med 176:799-809. 1992
    ..mAbs were directed at the outer membrane proteins, OspA or OspB...