major cold shock protein
Gene Symbol: major cold shock protein
Description: cold shock protein CspB
Species: Bacillus subtilis subsp. subtilis str. 168
Schindelin H, Marahiel M, Heinemann U. Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein. Nature. 1993;364:164-8 pubmed
..Preferential binding of CspB to single-stranded DNA is observed in gel retardation experiments. ..
Schnuchel A, Wiltscheck R, Czisch M, Herrler M, Willimsky G, Graumann P, et al
. Structure in solution of the major cold-shock protein from Bacillus subtilis. Nature. 1993;364:169-71 pubmed
..CspB binds to single-stranded DNA in gel retardation experiments. ..
Graumann P, Schroder K, Schmid R, Marahiel M. Cold shock stress-induced proteins in Bacillus subtilis. J Bacteriol. 1996;178:4611-9 pubmed
..after cold shock were highly homologous and belonged to a protein family of which one member, the major cold shock protein, CspB, has previously been characterized...
Schindelin H, Herrler M, Willimsky G, Marahiel M, Heinemann U. Overproduction, crystallization, and preliminary X-ray diffraction studies of the major cold shock protein from Bacillus subtilis, CspB. Proteins. 1992;14:120-4 pubmed
The major cold shock protein from Bacillus subtilis (CspB) was overexpressed using the bacteriophage T7 RNA polymerase/promoter system and purified to apparent homogeneity from recombinant Escherichia coli cells...
Francis K, Stewart G. Detection and speciation of bacteria through PCR using universal major cold-shock protein primer oligomers. J Ind Microbiol Biotechnol. 1997;19:286-93 pubmed
..Sequence analysis of the amplified products confirmed a high level of DNA homology. Significantly, however, there are sufficient nucleotide variations to allow the unique allocation of each amplified sequence to its parental bacterium...
Max K, Zeeb M, Bienert R, Balbach J, Heinemann U. T-rich DNA single strands bind to a preformed site on the bacterial cold shock protein Bs-CspB. J Mol Biol. 2006;360:702-14 pubmed publisher
Max K, Wunderlich M, Roske Y, Schmid F, Heinemann U. Optimized variants of the cold shock protein from in vitro selection: structural basis of their high thermostability. J Mol Biol. 2007;369:1087-97 pubmed publisher
..The stabilizing mutations are clustered at a contiguous surface area of Bs-CspB, which apparently is critically important for the stability of the beta-barrel structure but not well optimized in the wild-type protein...
Graumann P, Marahiel M. The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides. FEBS Lett. 1994;338:157-60 pubmed
We have characterized the nucleic acid binding properties of the major cold shock protein of Bacillus subtilis, CspB...
Noback M, Terpstra P, Holsappel S, Venema G, Bron S. A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the Bacillus subtilis chromosome. Microbiology. 1996;142 ( Pt 11):3021-6 pubmed
..The location of cspB in this sequence does not confirm the genetic mapping data, indicating that the gene is closely linked to comK, which is located at 80 degrees on the B. subtilis chromosome. ..