major cold shock protein

Summary

Gene Symbol: major cold shock protein
Description: cold shock protein CspB
Species: Bacillus subtilis subsp. subtilis str. 168

Top Publications

  1. Schindelin H, Marahiel M, Heinemann U. Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein. Nature. 1993;364:164-8 pubmed
    ..Preferential binding of CspB to single-stranded DNA is observed in gel retardation experiments. ..
  2. Schnuchel A, Wiltscheck R, Czisch M, Herrler M, Willimsky G, Graumann P, et al. Structure in solution of the major cold-shock protein from Bacillus subtilis. Nature. 1993;364:169-71 pubmed
    ..CspB binds to single-stranded DNA in gel retardation experiments. ..
  3. Graumann P, Schroder K, Schmid R, Marahiel M. Cold shock stress-induced proteins in Bacillus subtilis. J Bacteriol. 1996;178:4611-9 pubmed
    ..after cold shock were highly homologous and belonged to a protein family of which one member, the major cold shock protein, CspB, has previously been characterized...
  4. Schindelin H, Herrler M, Willimsky G, Marahiel M, Heinemann U. Overproduction, crystallization, and preliminary X-ray diffraction studies of the major cold shock protein from Bacillus subtilis, CspB. Proteins. 1992;14:120-4 pubmed
    The major cold shock protein from Bacillus subtilis (CspB) was overexpressed using the bacteriophage T7 RNA polymerase/promoter system and purified to apparent homogeneity from recombinant Escherichia coli cells...
  5. Francis K, Stewart G. Detection and speciation of bacteria through PCR using universal major cold-shock protein primer oligomers. J Ind Microbiol Biotechnol. 1997;19:286-93 pubmed
    ..Sequence analysis of the amplified products confirmed a high level of DNA homology. Significantly, however, there are sufficient nucleotide variations to allow the unique allocation of each amplified sequence to its parental bacterium...
  6. Max K, Zeeb M, Bienert R, Balbach J, Heinemann U. T-rich DNA single strands bind to a preformed site on the bacterial cold shock protein Bs-CspB. J Mol Biol. 2006;360:702-14 pubmed publisher
  7. Max K, Wunderlich M, Roske Y, Schmid F, Heinemann U. Optimized variants of the cold shock protein from in vitro selection: structural basis of their high thermostability. J Mol Biol. 2007;369:1087-97 pubmed publisher
    ..The stabilizing mutations are clustered at a contiguous surface area of Bs-CspB, which apparently is critically important for the stability of the beta-barrel structure but not well optimized in the wild-type protein...
  8. Graumann P, Marahiel M. The major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides. FEBS Lett. 1994;338:157-60 pubmed
    We have characterized the nucleic acid binding properties of the major cold shock protein of Bacillus subtilis, CspB...
  9. Noback M, Terpstra P, Holsappel S, Venema G, Bron S. A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the Bacillus subtilis chromosome. Microbiology. 1996;142 ( Pt 11):3021-6 pubmed
    ..The location of cspB in this sequence does not confirm the genetic mapping data, indicating that the gene is closely linked to comK, which is located at 80 degrees on the B. subtilis chromosome. ..

More Information

Publications13

  1. Sachs R, Max K, Heinemann U, Balbach J. RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution. RNA. 2012;18:65-76 pubmed publisher
    ..The generally lower binding affinity of ssRNA compared to its ssDNA analog is attributed solely to the substitution of thymine by uracil bases in RNA...
  2. Willimsky G, Bang H, Fischer G, Marahiel M. Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. J Bacteriol. 1992;174:6326-35 pubmed
    ..Nucleotide and deduced amino acid sequence comparisons revealed 61% identity to the major cold shock protein of E. coli and 43% identity to a family of eukaryotic DNA binding proteins...
  3. Zeeb M, Max K, Weininger U, L w C, Sticht H, Balbach J. Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution. Nucleic Acids Res. 2006;34:4561-71 pubmed publisher
    ..15N NMR relaxation and H/D exchange experiments revealed that binding of dT7 increases the stability of Bs-CspB and reduces the sub-nanosecond dynamics of the entire protein and especially of loop beta3-beta4...
  4. Schroder K, Zuber P, Willimsky G, Wagner B, Marahiel M. Mapping of the Bacillus subtilis cspB gene and cloning of its homologs in thermophilic, mesophilic and psychrotrophic bacilli. Gene. 1993;136:277-80 pubmed