Gene Symbol: hag
Species: Bacillus subtilis subsp. subtilis str. 168
Asano Y, Onishi H, Tajima K, Shinozawa T. Flagellin as a biomarker for Bacillus subtilis strains; application to the DB9011 strain and the study of interspecific diversity in amino-acid sequences. Biosci Biotechnol Biochem. 2001;65:1218-22 pubmed
..Cross-reactions were observed in 4 B. subtilis strains with similar flagellin genes (hag). Alignment of partial amino-acid sequences of flagellin and the results of PCR for the 16S/23S rRNA spacer in 11 B...
Yakhnin H, Pandit P, Petty T, Baker C, Romeo T, Babitzke P. CsrA of Bacillus subtilis regulates translation initiation of the gene encoding the flagellin protein (hag) by blocking ribosome binding. Mol Microbiol. 2007;64:1605-20 pubmed publisher
..b>hag, the gene encoding flagellin, was identified as a CsrA-regulated gene...
DeLange R, Chang J, Shaper J, Glazer A. Amino acid sequence of flagellin of Bacillus subtilis 168. III. Tryptic peptides, N-bromosuccinimide peptides, and the complete amino acid sequence. J Biol Chem. 1976;251:705-11 pubmed
..The NH2-terminal region (residues 1 to 101) possesses a net charge of 6 plus, the middle of the molecule (residues 102 to 203), a net charge of 9 minus, and the COOH terminal region (residues 204 to 304), a net charge of 4 minus. ..
Mirel D, Chamberlin M. The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase. J Bacteriol. 1989;171:3095-101 pubmed
The Bacillus subtilis gene hag, which codes for the flagellin structural protein, was identified by DNA sequence analysis in a collection of DNA fragments bearing in vitro promoters for the sigma 28 form of RNA polymerase...
Lavallie E, Stahl M. Cloning of the flagellin gene from Bacillus subtilis and complementation studies of an in vitro-derived deletion mutation. J Bacteriol. 1989;171:3085-94 pubmed
..chromosomal locus containing the wild-type flagellin allele was replaced with a defective allele of the gene (delta hag-633) that contained a 633-base-pair deletion...
Antelmann H, Bernhardt J, Schmid R, Hecker M. A gene at 333 degrees on the Bacillus subtilis chromosome encodes the newly identified sigma B-dependent general stress protein GspA. J Bacteriol. 1995;177:3540-5 pubmed
..In gspA mutant cells, the level of flagellin was increased severalfold over that in wild-type cells. ..
Soldo B, Lazarevic V, Mauel C, Karamata D. Sequence of the 305 degrees-307 degrees region of the Bacillus subtilis chromosome. Microbiology. 1996;142 ( Pt 11):3079-88 pubmed
..The products of the sequenced genes are involved in the regulation of degradative enzymes, competence, flagellar motility and protein secretion. Putative functions of newly identified genes are based on sequence homologies. ..
Kodgire P, Rao K. hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC. Microbiology. 2009;155:142-9 pubmed publisher
In Bacillus subtilis, motility and chemotaxis require the expression of hag, which encodes flagellin...