Gene Symbol: glnA
Description: glutamine synthetase
Species: Bacillus subtilis subsp. subtilis str. 168
Strauch M, Aronson A, Brown S, Schreier H, Sonenhein A. Sequence of the Bacillus subtilis glutamine synthetase gene region. Gene. 1988;71:257-65 pubmed
..Two downstream ORFs are separated from glnA by an apparent Rho-independent termination site...
Fisher S, Wray L. Novel trans-Acting Bacillus subtilis glnA mutations that derepress glnRA expression. J Bacteriol. 2009;191:2485-92 pubmed publisher
..in this mutant screen all contained mutations tightly linked to the glnRA operon which encodes GlnR (glnR) and GS (glnA). Four Gln(C) mutants contained mutations in the glnR gene that most likely impair the ability of GlnR to bind DNA...
Wray L, Fisher S. Functional roles of the conserved Glu304 loop of Bacillus subtilis glutamine synthetase. J Bacteriol. 2010;192:5018-25 pubmed publisher
..A model for the role of the Glu304 residue in glutamine feedback inhibition is proposed...
Fedorova K, Kayumov A, Woyda K, Ilinskaja O, Forchhammer K. Transcription factor TnrA inhibits the biosynthetic activity of glutamine synthetase in Bacillus subtilis. FEBS Lett. 2013;587:1293-8 pubmed publisher
..The transcription factor TnrA inhibits the biosynthetic activity of glutamine synthetase in vivo and in vitro, while the GlnR protein does not affect the activity of the enzyme. ..
Hauf K, Kayumov A, Gloge F, Forchhammer K. The Molecular Basis of TnrA Control by Glutamine Synthetase in Bacillus subtilis. J Biol Chem. 2016;291:3483-95 pubmed publisher
..The highly interdependent ligand binding properties of GS reveal this enzyme as a sophisticated sensor of the nitrogen and energy state of the cell to control the activity of DNA-bound TnrA. ..
Wray L, Zalieckas J, Fisher S. Bacillus subtilis glutamine synthetase controls gene expression through a protein-protein interaction with transcription factor TnrA. Cell. 2001;107:427-35 pubmed
..Thus, glutamine synthetase, an enzyme of central metabolism, directly interacts with and regulates the DNA binding activity of TnrA. ..
Nakano Y, Tanaka E, Kato C, Kimura K, Horikoshi K. The complete nucleotide sequence of the glutamine synthetase gene (glnA) of Bacillus subtilis. FEMS Microbiol Lett. 1989;48:81-6 pubmed
..A plasmid, pSGS2, was isolated from a glnA+ transformant and the cloned GS gene was found to be located in a 3.6 kb DNA fragment...
Wray L, Fisher S. Bacillus subtilis GlnR contains an autoinhibitory C-terminal domain required for the interaction with glutamine synthetase. Mol Microbiol. 2008;68:277-85 pubmed publisher
..Compared with the full-length GlnR protein, the truncated GlnR proteins were defective in their interaction with feedback-inhibited glutamine synthetase in cross-linking experiments...