Gene Symbol: BC0670
Description: phospholipase C
Species: Bacillus cereus ATCC 14579
Hansen S, Hough E, Svensson L, Wong Y, Martin S. Crystal structure of phospholipase C from Bacillus cereus complexed with a substrate analog. J Mol Biol. 1993;234:179-87 pubmed
..A water molecule, located approximately apical to the diacylglycerol leaving group, seems to be the most likely candidate for the attacking nucleophile which initiates the reaction. ..
Hansen S, Hansen L, Hough E. The crystal structure of tris-inhibited phospholipase C from Bacillus cereus at 1.9 A resolution. The nature of the metal ion in site 2. J Mol Biol. 1993;231:870-6 pubmed
..This study is one of a series which explores the active site of PLC by complexing the enzyme with inhibitors and substrate analogues. ..
Hansen S, Hansen L, Hough E. Crystal structures of phosphate, iodide and iodate-inhibited phospholipase C from Bacillus cereus and structural investigations of the binding of reaction products and a substrate analogue. J Mol Biol. 1992;225:543-9 pubmed
..The ninth blocks the entrance to the active site cleft and is of higher occupancy. Altogether, these results suggest that the substrate, a phospholipid, is associated directly with the metal ions during catalysis...
Gilmore M, Cruz Rodz A, Leimeister W chter M, Kreft J, Goebel W. A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage. J Bacteriol. 1989;171:744-53 pubmed
..subtilis host. The 3' open reading frame encoded a sphingomyelinase. The two tandemly encoded activities, phospholipase C and sphingomyelinase, constitute a biologically functional cytolytic determinant of B. cereus termed cereolysin AB...
Antikainen N, Monzingo A, Franklin C, Robertus J, Martin S. Using X-ray crystallography of the Asp55Asn mutant of the phosphatidylcholine-preferring phospholipase C from Bacillus cereus to support the mechanistic role of Asp55 as the general base. Arch Biochem Biophys. 2003;417:81-6 pubmed
..These observations demonstrate that Asp55 is not critical for the structural integrity of the enzyme and support the hypothesis that Asp55 is the general base in the PLC(Bc)-catalyzed hydrolysis of phospholipids. ..