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Genomes and GenesSpecies | ADA2SummaryGene Symbol: ADA2 Description: Ada2p Alias: SWI8 Species: Top Publications
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A role for Gcn5 in replication-coupled nucleosome assemblyRebecca J Burgess
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
Mol Cell 37:469-80. 2010..These results demonstrate that Gcn5 regulates RC nucleosome assembly, in part, by promoting H3 association with CAF-1 via H3 acetylation...- Role of the Ada2 and Ada3 transcriptional coactivators in histone acetylationRamakrishnan Balasubramanian
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 1014, USA
J Biol Chem 277:7989-95. 2002..In this work, we examine the roles of the Ada2 and Ada3 coactivator proteins that are functionally linked to Gcn5... - The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11Alwin Köhler
Biochemie Zentrum der Universitat Heidelberg, INF328, D 69120, Heidelberg, Germany
Mol Biol Cell 17:4228-36. 2006..Interestingly, sgf11 deletion enhances the mRNA export defect observed in sus1delta cells. Thus, the Sus1-Sgf11-Ubp8 module could work at the junction between SAGA-dependent transcription and nuclear mRNA export... - Characterization of physical interactions of the putative transcriptional adaptor, ADA2, with acidic activation domains and TATA-binding proteinN A Barlev
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 270:19337-44. 1995..We tested physical interactions of the putative adaptor ADA2 with activation domains derived from acidic activator proteins and with certain general transcription factors... - Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promotersR Belotserkovskaya
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Mol Cell Biol 20:634-47. 2000..These results suggest that the composition of SAGA may be dynamic in vivo and may be regulated through dissociable inhibitory subunits... - The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiaeA Eberharter
Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
Mol Cell Biol 19:6621-31. 1999..By a combination of mass spectrometry analysis and immunoblotting, we demonstrate that the adapter proteins Ada2, Ada3, and Gcn5 are indeed integral components of ADA. Furthermore, we identify the product of the S... - SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitmentDecha Sermwittayawong
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA
EMBO J 25:3791-800. 2006..This simple model can explain SAGA's observed ability to both activate and repress transcription... - A conserved central region of yeast Ada2 regulates the histone acetyltransferase activity of Gcn5 and interacts with phospholipidsStephen M T Hoke
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Canada
J Mol Biol 384:743-55. 2008..acetyltransferase, Gcn5, preferentially acetylates histones H3 and H2B and is regulated through interactions with Ada2 and Ngg1/Ada3... - The ATM-related cofactor Tra1 is a component of the purified SAGA complexP A Grant
Howard Hughes Medical Institute, Pennsylvania State University, University Park 16802 4500, USA
Mol Cell 2:863-7. 1998..These results indicate a role for Tra1 in the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter... - Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA exportAlwin Köhler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Nat Cell Biol 10:707-15. 2008..Loss of Sgf73 from SAGA abrogates gene gating of GAL1 and causes a GAL1 mRNA export defect. Thus, Sgf73 provides a molecular scaffold to integrate the regulation of H2B ubiquitin levels, tethering of a gene to the NPC and export of mRNA... - Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8Karl W Henry
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Genes Dev 17:2648-63. 2003..These results suggest that the histone H2B ubiquitylation state is dynamic during transcription, and that the sequence of histone modifications helps to control transcription... - The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complexKenneth K Lee
Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
Mol Cell Biol 25:1173-82. 2005..Taken together, these data indicate that the expression of some genes, including ARG1, is regulated by a balance of histone H2B ubiquitylation in the cell... - Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylationMarilyn G Pray-Grant
Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
Nature 433:434-8. 2005..Our study identifies the first chromodomain that recognizes methylated histone H3 (Lys 4) and possibly identifies a larger subfamily of chromodomain proteins with similar recognition properties... - Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunitsK K Lee
Stowers Institute for Medical Research, 1000 E 50th Kansas City, MO 64110, USA
Biochem Soc Trans 32:899-903. 2004..Our analysis identified two novel proteins, one associated with SAGA, YPL047W (Sgf11), and another associated with Swi/Snf, Rtt102... - Cluster analysis of mass spectrometry data reveals a novel component of SAGADavid W Powell
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232 2363, USA
Mol Cell Biol 24:7249-59. 2004..Our data suggest that the role of SGF11 in transcription is independent of SAGA's histone acetyltransferase activity but may involve Ubp8p recruitment to or stabilization in SAGA... - Molecular architecture of the S. cerevisiae SAGA complexPei Yun Jenny Wu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell 15:199-208. 2004..Our data provide insights into the molecular architecture of SAGA and imply a functional organization to the complex... - SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcriptionDavid E Sterner
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 99:11622-7. 2002..These data clarify a mechanism by which a transcriptional regulatory complex can switch between positive and negative modulation... - Deubiquitination of histone H2B by a yeast acetyltransferase complex regulates transcriptionJeremy A Daniel
Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Biol Chem 279:1867-71. 2004..Collectively, these data suggest that the SAGA and SLIK HAT complexes can regulate an integrated set of multiple histone modifications, counteracting repressive effects that alter chromatin and regulate gene expression... - The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathwayMarilyn G Pray-Grant
Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
Mol Cell Biol 22:8774-86. 2002.... - Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interactionD E Sterner
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Mol Cell Biol 19:86-98. 1999..Specifically, null mutations in the Gcn5/Ada2/Ada3 or Spt3/Spt8 classes cause moderate phenotypes and subtle structural alterations, while mutations in a third .. - Functional similarity and physical association between GCN5 and ADA2: putative transcriptional adaptorsG A Marcus
Department of Biology, MIT, Cambridge 02139
EMBO J 13:4807-15. 1994A selection for yeast mutants resistant to GAL4-VP16-induced toxicity previously identified two genes, ADA2 and ADA3, which may function as adaptors for some transcriptional activation domains and thereby facilitate activation... - Structural and functional analysis of yeast putative adaptors. Evidence for an adaptor complex in vivoR Candau
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 271:5237-45. 1996..To study their functional interactions, deletion mutations in the yeast adaptors ADA2, GCN5, and ADA3 were created... - Tra1p is a component of the yeast Ada.Spt transcriptional regulatory complexesA Saleh
Department of Biochemistry, University of Western Ontario, London N6A 5C1, Canada
J Biol Chem 273:26559-65. 1998..Despite the similarity of Tra1p to a group of putative protein kinases, we have not detected protein kinase activity within immunoprecipitates of Tra1p or the Ada.Spt complexes... - ADA3, a putative transcriptional adaptor, consists of two separable domains and interacts with ADA2 and GCN5 in a trimeric complexJ Horiuchi
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
Mol Cell Biol 15:1203-9. 1995Mutations in yeast ADA2, ADA3, and GCN5 weaken the activation potential of a subset of acidic activation domains... - Identification of native complexes containing the yeast coactivator/repressor proteins NGG1/ADA3 and ADA2A Saleh
Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
J Biol Chem 272:5571-8. 1997..This supports a role for NGG1p in the interaction with TBP and suggests that the interaction with TBP is functionally relevant... - A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulationP A Grant
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
Cell 94:45-53. 1998..These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145... - Analysis of Spt7 function in the Saccharomyces cerevisiae SAGA coactivator complexPei Yun Jenny Wu
Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Mol Cell Biol 22:5367-79. 2002..Analysis of an spt7 mutant with greatly reduced levels of SLIK/SAGA(alt)/SALSA suggests a subtle role for this complex in transcription that may be redundant with a subset of SAGA functions... - ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3J Horiuchi
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Mol Cell Biol 17:3220-8. 1997..Thus, the ADAs can be separated into two classes, suggesting that the ADA/GCN5 complex may have two separate functions. We present a speculative model on the possible roles of the ADA/GCN5 complex... - Transcriptional activators direct histone acetyltransferase complexes to nucleosomesR T Utley
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802 4500, USA
Nature 394:498-502. 1998..Our results demonstrate the targeting of native HAT complexes by a transcription-activation domain to nucleosomes in order to activate transcription... - Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexesBruce A Knutson
Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, WA 98109, USA
Mol Cell Biol 31:818-31. 2011..Our results show that Tra1 recruitment at Gcn4-dependent and Rap1-dependent promoters requires the same regions of Tra1 and that separate regions of Tra1 contribute to the HAT activity and stability of the SAGA and NuA4 HAT modules... - Mpk1 MAPK association with the Paf1 complex blocks Sen1-mediated premature transcription terminationKi Young Kim
Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
Cell 144:745-56. 2011..Finally, we demonstrate that this mechanism is conserved in an interaction between the human ERK5 MAPK and human Paf1... - Identification of Pep4p as the protease responsible for formation of the SAGA-related SLIK protein complexGianpiero Spedale
Department of Physiological Chemistry, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
J Biol Chem 285:22793-9. 2010..Strains mimicking constitutive SLIK formation showed increased resistance to rapamycin treatment, suggesting a role for SLIK in regulating cellular responses to nutrient stress... - Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase moduleAlwin Köhler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Cell 141:606-17. 2010..Our structural and functional analyses reveal a central role of Sgf11 and Sgf73 in activating Ubp8 for deubiquitinating histone H2B and demonstrate how a DUB can be allosterically regulated by its nonsubstrate partners... - Mutational analysis of the C-terminal FATC domain of Saccharomyces cerevisiae Tra1Stephen M T Hoke
Department of Biochemistry, University of Western Ontario, London, Canada
Curr Genet 56:447-65. 2010..These findings demonstrate the regulatory potential of mechanisms targeting the FATC domains of PIKK proteins... - The Gcn5 bromodomain of the SAGA complex facilitates cooperative and cross-tail acetylation of nucleosomesShanshan Li
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
J Biol Chem 284:9411-7. 2009.... - Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexesKenneth K Lee
Stowers Institute for Medical Research, 50th Street Kansas City, MO 64110, USA
Epigenetics Chromatin 2:2. 2009.... - Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2Pau Pascual-García
Centro de Investigacion Principe Felipe, E 46012 Valencia, Spain
Genes Dev 22:2811-22. 2008..Our results reveal that Sus1 plays a key role in coordinating gene transcription and mRNA export by working at the interface between the SAGA and TREX2 complexes during transcription elongation... - Acetylation of Rsc4p by Gcn5p is essential in the absence of histone H3 acetylationJennifer K Choi
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
Mol Cell Biol 28:6967-72. 2008..These results demonstrate for the first time the vital and yet redundant functions of histone H3 and Rsc4p acetylation in maintaining cell viability... - Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylationChuanbing Bian
School of Life Sciences, University of Science and Technology of China, Anhui, People s Republic of China
EMBO J 30:2829-42. 2011..Our in vitro and in vivo functional assays show that Sgf29 recognizes methylated H3K4 to recruit the SAGA complex to its targets sites and mediates histone H3 acetylation, underscoring the importance of Sgf29 in gene regulation... - C-terminal processing of yeast Spt7 occurs in the absence of functional SAGA complexStephen M T Hoke
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, N6A5C1, Canada
BMC Biochem 8:16. 2007..Two forms of Spt7 have been identified, the second of which is truncated at its C-terminus and found in the SAGA-like (SLIK) complex... - Selective requirement for SAGA in Hog1-mediated gene expression depending on the severity of the external osmostress conditionsMeritxell Zapater
Cell Signaling Unit, Departament de Ciencies Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomedica de Barcelona, Dr Aiguader 88, E 08003 Barcelona, Spain
Mol Cell Biol 27:3900-10. 2007.... - Acetylation by the transcriptional coactivator Gcn5 plays a novel role in co-transcriptional spliceosome assemblyFelizza Q Gunderson
Department of Biology, Molecular Biology Section, University of California San Diego, La Jolla, California, USA
PLoS Genet 5:e1000682. 2009..These results demonstrate a novel role for acetylation by SAGA in co-transcriptional recruitment of the U2 snRNP and recognition of the intron branchpoint... - Identification and characterization of the activation domain of Ifh1, an activator of model TATA-less genesPeipei Zhong
School of Biomedical Sciences, University of Ulster, Coleraine, UK
Biochem Biophys Res Commun 392:77-82. 2010.... - Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 geneJ A Ferreiro
Department of Functional Biology, University of Oviedo Oviedo 33006, Spain
Nucleic Acids Res 34:976-85. 2006..Deletion of GCN5 or ADA2 reduces repair at MET16... - A targeted histone acetyltransferase can create a sizable region of hyperacetylated chromatin and counteract the propagation of transcriptionally silent chromatinYa Hui Chiu
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588, USA
Genetics 165:115-25. 2003..The anti-silencing effect of Gcn5p is abolished by a mutation that eliminated its HAT activity or by deleting the ADA2 gene encoding a structural component of Gcn5p-containing HAT complexes... - Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexesM S Santisteban
Department of Microbiology and Cancer Center, University of Virginia, Charlottesville 22908, USA
Cell 103:411-22. 2000..These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure... - Histone acetyltransferase activity and interaction with ADA2 are critical for GCN5 function in vivoR Candau
The Wistar Institute, Philadelphia, PA 19104, USA
EMBO J 16:555-65. 1997Yeast GCN5 is one component of a putative adaptor complex that includes ADA2 and ADA3 and functionally connects DNA-bound transcriptional activators with general transcription factors... - The essential function of Not1 lies within the Ccr4-Not complexL Maillet
Departement de Biochimie Medicale, Centre Medical Universitaire, 1211, Geneva 4, Switzerland
J Mol Biol 303:131-43. 2000..These results are consistent with the essential function of Not1p lying within the Ccr4-Not complex... - The transcriptional co-activator ADA5 is required for HAC1 mRNA processing in vivoA A Welihinda
Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109 0650, USA
J Biol Chem 275:3377-81. 2000..Subunits of the yeast transcriptional co-activator complex SAGA also play a role in the UPR. Deletion of GCN5, ADA2, or ADA3 reduces, and deletion of ADA5 completely abolishes, the UPR... - Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoterM P Cosma
Research Institute of Molecular Pathology, Vienna, Austria
Cell 97:299-311. 1999..Swi5p remains at HO for only 5 min. Swi/Snf's and SAGA's subsequent persistence at HO is self sustaining and constitutes an "epigenetic memory" of HO's transient interaction with Swi5p... - Expanded lysine acetylation specificity of Gcn5 in native complexesP A Grant
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
J Biol Chem 274:5895-900. 1999..Furthermore Ada and SAGA have overlapping, yet distinct, patterns of acetylation, suggesting that the association of specific subunits determines site specificity... - Role for ADA/GCN5 products in antagonizing chromatin-mediated transcriptional repressionK J Pollard
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01605, USA
Mol Cell Biol 17:6212-22. 1997..We show here that mutations in ADA2, ADA3, and GCN5, which are believed to encode subunits of a nuclear histone acetyltransferase complex, cause .. - Association of distinct yeast Not2 functional domains with components of Gcn5 histone acetylase and Ccr4 transcriptional regulatory complexesJ D Benson
Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
EMBO J 17:6714-22. 1998..Loss of this interaction either through abrogation of Not2 N-terminal function or deletion of ada2 or gcn5 results in derepression of the HIS3 TC element... - The Gcn4p activation domain interacts specifically in vitro with RNA polymerase II holoenzyme, TFIID, and the Adap-Gcn5p coactivator complexC M Drysdale
Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
Mol Cell Biol 18:1711-24. 1998.... - The SANT domain of Ada2 is required for normal acetylation of histones by the yeast SAGA complexDavid E Sterner
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 277:8178-86. 2002..We have analyzed deletion and substitution mutations within Gcn5 and Ada2, an interacting protein within SAGA, and have identified substrate recognition functions within the SANT domain of .. - The Rpb9 subunit of RNA polymerase II binds transcription factor TFIIE and interferes with the SAGA and elongator histone acetyltransferasesVincent Van Mullem
Laboratoire de Genetique Moleculaire, Facultes Universitaires Notre Dame de la Paix, 61 rue de Bruxelles, B 5000 Namur, Belgique
J Biol Chem 277:10220-5. 2002..Tfa1 is immunoprecipitated by RNA polymerase II. This co-purification is strongly reduced in rpb9-Delta, suggesting that Rpb9 contributes to the recruitment of TFIIE on RNA polymerase II... - Yeast ADA2 protein binds to the VP16 protein activation domain and activates transcriptionN Silverman
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
Proc Natl Acad Sci U S A 91:11665-8. 1994Previously it was shown that yeast ADA2 protein is necessary for the full activity of some activation domains, such as VP16 and GCN4, in vivo and in vitro... - Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1Manolis Papamichos-Chronakis
Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Biology Department, University of Crete, P O Box 1527, Vassilika Vouton, 711 10 Heraklion, Crete, Greece
Mol Cell 9:1297-305. 2002..Thus, corepressor's release is not the mechanism of derepression; instead, it is the coactivator complex that alleviates Cyc8-Tup1-mediated repression under induction conditions... - ADA3-containing complexes associate with estrogen receptor alphaArndt Benecke
Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, College de France, BP163, 67404 Illkirch Cedex, France
Nucleic Acids Res 30:2508-14. 2002..However, the ADA3-containing TBP-free-TAF-containing complex (TFTC) can interact with ERalpha in a ligand-independent manner, indicating that other subunits of the complex are sufficient to mediate interaction with NRs... - Gal80 confers specificity on HAT complex interactions with activatorsMichael J Carrozza
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA
J Biol Chem 277:24648-52. 2002..These studies demonstrate that the specificity of HAT/activator interactions can be dictated by cofactors that modulate activation domain function in response to cellular signals... - Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patternsDoris B Kirschner
Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, F 67404 Illkirch Cedex, CU de Strasbourg, France
Mol Cell Biol 22:3178-93. 2002..Thus, different yTAF(II)25 mutations induce distinct phenotypes and affect the regulation of different subsets of genes, demonstrating that no individual TAF(II) mutant allele reflects the full range of its normal functions... - Identification of human proteins functionally conserved with the yeast putative adaptors ADA2 and GCN5R Candau
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Mol Cell Biol 16:593-602. 1996..Consistent with this view, we have identified possible human homologs of yeast ADA2 (yADA2) and yeast GCN5 (yGCN5), components of a putative adaptor complex... - Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complexP A Grant
Department of Biochemistry and Molecular Biology and The Center for Gene Regulation, The Pennsylvania State University, University Park 16802 4500, USA
Genes Dev 11:1640-50. 1997..Both the 0.8- and 1.8-MD Gcn5-containing complexes cofractionate with Ada2 and are lost in gcn5delta, ada2delta, or ada3delta yeast strains, illustrating that these HAT complexes are bona .. - ADR1 activation domains contact the histone acetyltransferase GCN5 and the core transcriptional factor TFIIBY C Chiang
Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, New Hampshire 03824, USA
J Biol Chem 271:32359-65. 1996..Mutations in ADA2 or GCN5, encoding components of the ADA coactivator complex involved in histone acetylation, severely reduced LexA-.. - GAL4 interacts with TATA-binding protein and coactivatorsK Melcher
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235 8573, USA
Mol Cell Biol 15:2839-48. 1995..crude extract: the negative regulator GAL80, the TATA-binding protein (TBP), and the putative coactivators SUG1 and ADA2. TFIIB was not retained... - Histone acetyltransferase activity is conserved between yeast and human GCN5 and is required for complementation of growth and transcriptional activationL Wang
Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Mol Cell Biol 17:519-27. 1997Yeast and human ADA2 and GCN5 (y- and hADA2 and y- and hGCN5, respectively) have been shown to potentiate transcription in vivo and may function as adaptors to bridge physical interactions between DNA-bound activators and the basal .. - yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complexK Natarajan
Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
Mol Cell 2:683-92. 1998..Our results provide strong evidence that recruitment of SAGA, in addition to holoenzyme, is crucial for activation by Gcn4p in vivo and that yTAFII61 plays a key role in this process... - The SAGA subunit Ada2 functions in transcriptional silencingSandra Jacobson
Division of Biological Sciences, University of California at San Diego, 9500 Gilman Dr, 0347, San Diego, CA 92093, USA
Mol Cell Biol 29:6033-45. 2009The cellular role of the Ada2 coactivator is currently understood in the context of the SAGA histone acetyltransferase (HAT) complex, where Ada2 increases the HAT activity of Gcn5 and interacts with transcriptional activators... - The role of histone ubiquitylation and deubiquitylation in gene expression as determined by the analysis of an HTB1(K123R) Saccharomyces cerevisiae strainA Irina Mutiu
Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Canada
Mol Genet Genomics 277:491-506. 2007..Finally, by comparing gene expression changes in the htb1 ( K123R ) strain with those in a strain deleted for rad6, we conclude that lysine 123 affects transcription primarily because of it being a site of ubiquitylation... - Molecular genetic dissection of TAF25, an essential yeast gene encoding a subunit shared by TFIID and SAGA multiprotein transcription factorsJ Kirchner
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0615, USA
Mol Cell Biol 21:6668-80. 2001..The results of these analyses show that TAF25p is comprised of multiple mutable elements which contribute importantly to RNA polymerase II-mediated mRNA gene transcription... - Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4pHongfang Qiu
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development NIH, Building 6A, Bethesda, MD 20892, USA
Mol Cell Biol 25:3461-74. 2005..Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo... - Adenovirus E1A requires the yeast SAGA histone acetyltransferase complex and associates with SAGA components Gcn5 and Tra1Caroline A Kulesza
Department of Microbiology and Cancer Center, University of Virginia School of Medicine, Charlottesville, Virginia, VA 22908, USA
Oncogene 21:1411-22. 2002..Transcriptional activation by Adr1 requires interaction with co-activator proteins Ada2 and Gcn5, components of histone acetyltransferase complexes including ADA and SAGA... - Autonomous function of the amino-terminal inhibitory domain of TAF1 in transcriptional regulationShinya Takahata
Division of Molecular and Cellular Biology, Graduate School of Integrated Science, Yokohama City University, Yokohama 230 0045, Japan
Mol Cell Biol 24:3089-99. 2004..These results indicate that there is no or minimal geometric constraint on the ability of the TAND to function normally in transcriptional regulation as long as TFIID assembly is secured... - Essential role for the SANT domain in the functioning of multiple chromatin remodeling enzymesLaurie A Boyer
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cell 10:935-42. 2002..Our results are consistent with a general role for SANT domains in functional interactions with histone N-terminal tails... - Acetylation of histones and transcription-related factorsD E Sterner
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
Microbiol Mol Biol Rev 64:435-59. 2000..Thus, we also detail these known factor acetyltransferase (FAT) substrates and the demonstrated or potential roles of their acetylation in transcriptional processes...
Research Grants
- MECHANISMS OF TRANSCRIPTIONAL REGULATION IN CHROMATINJerry Workman; Fiscal Year: 2007..Finally, we will investigate mechansims by which SWI/SNF may act in concert with histone deacetylases to facilitate transcriptional repression. ..