pepC

Summary

Gene Symbol: pepC
Description: serine proteinase pepC-Aspergillus niger
Species:

Research Grants

  1. Protein Folding in the Eukaryotic Cytosol
    Judith Frydman; Fiscal Year: 2007
  2. Principles in Cardiovascular Research Training Program
    Anthony J Muslin; Fiscal Year: 2008
  3. MAP Kinase and AKT Signaling in Congestive Heart Failure
    Anthony J Muslin; Fiscal Year: 2008
  4. RGS Protein Function in Cardiac Physiology
    Anthony J Muslin; Fiscal Year: 2008
  5. Protein Folding in the Eukaryotic Cytosol
    Judith Frydman; Fiscal Year: 2008
  6. Mechanisms of COPII-Dependent Transport
    CHARLES K BARLOWE; Fiscal Year: 2008
  7. Investigating the role of Ltv1 in 40S ribosome biogenesis and export
    Deborah E Lycan; Fiscal Year: 2006
  8. Principles in Cardiovascular Research Training Program
    Anthony J Muslin; Fiscal Year: 2007
  9. Mechanisms of COPII-Dependent Transport
    CHARLES K BARLOWE; Fiscal Year: 2007
  10. AUTOMATED PROTEIN FOLD MODEL GENERATION
    Temple F Smith; Fiscal Year: 2003

Publications

  1. Citrate, a possible precursor of astaxanthin in Phaffia rhodozyma: influence of varying levels of ammonium, phosphate and citrate in a chemically defined medium
    L B Flores Cotera
    Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF
    Appl Microbiol Biotechnol 55:341-7
  2. The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae
    F T Cooke
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    Curr Biol 8:1219-22
  3. Inversin, a novel gene in the vertebrate left-right axis pathway, is partially deleted in the inv mouse
    D Morgan
    Department of Human Genetics, University of Newcastle upon Tyne, UK
    Nat Genet 20:149-56
  4. Identification and functional analysis of hPRP17, the human homologue of the PRP17/CDC40 yeast gene involved in splicing and cell cycle control
    S Ben Yehuda
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
    RNA 4:1304-12
  5. Pasteurella haemolytica complicated respiratory infections in sheep and goats
    K A Brogden
    Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, IA 50010, USA
    Vet Res 29:233-54
  6. Identification of a tRNA-specific nuclear export receptor
    U Kutay
    Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany
    Mol Cell 1:359-69
  7. Characterization of the maleylacetate reductase MacA of Rhodococcus opacus 1CP and evidence for the presence of an isofunctional enzyme
    V Seibert
    Institut für Mikrobiologie, Universität Stuttgart, D 70569 Stuttgart, Germany
    J Bacteriol 180:3503-8
  8. Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans
    J F Staab
    Department of Medical Microbiology and Immunology, The Ohio State University College of Medicine, Columbus 43210 1239, USA
    Yeast 14:681-6
  9. The crystal structure of phenol hydroxylase in complex with FAD and phenol provides evidence for a concerted conformational change in the enzyme and its cofactor during catalysis
    C Enroth
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Structure 6:605-17
  10. The lissencephaly gene product Lis1, a protein involved in neuronal migration, interacts with a nuclear movement protein, NudC
    S M Morris
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Biol 8:603-6

Scientific Experts

Detail Information

Research Grants15

  1. Protein Folding in the Eukaryotic Cytosol
    Judith Frydman; Fiscal Year: 2007
    ..This abstract is not available...
  2. Principles in Cardiovascular Research Training Program
    Anthony J Muslin; Fiscal Year: 2008
    ..End of Abstract) Public Health Relevance: This Public Health Relevance is not available...
  3. MAP Kinase and AKT Signaling in Congestive Heart Failure
    Anthony J Muslin; Fiscal Year: 2008
    ..Our findings will help to identify specific signaling mechanisms involved in the regulation of cardiac myocyte growth and metabolism. Public Health Relevance: This Public Health Relevance is not available...
  4. RGS Protein Function in Cardiac Physiology
    Anthony J Muslin; Fiscal Year: 2008
    ..Public Health Relevance: This Public Health Relevance is not available...
  5. Protein Folding in the Eukaryotic Cytosol
    Judith Frydman; Fiscal Year: 2008
    ..Finally, our fourth specific aim will explore the interaction between chaperones and the translational machinery. Public Health Relevance: This Public Health Relevance is not available...
  6. Mechanisms of COPII-Dependent Transport
    CHARLES K BARLOWE; Fiscal Year: 2008
    ..Public Health Relevance: This Public Health Relevance is not available...
  7. Investigating the role of Ltv1 in 40S ribosome biogenesis and export
    Deborah E Lycan; Fiscal Year: 2006
    ..Understanding the mechanisms underlying ribosome biogenesis may thus provide new targets for limiting the growth of such rapidly dividing cells as cancerous tumor cells...
  8. Principles in Cardiovascular Research Training Program
    Anthony J Muslin; Fiscal Year: 2007
    ..Candidates for the 6 positions requested each year are selected based on past achievements and the likelihood that they will pursue an academic research career. (End of Abstract)..
  9. Mechanisms of COPII-Dependent Transport
    CHARLES K BARLOWE; Fiscal Year: 2007
    ..Therefore, the proposed studies are basic for understanding numerous health related issues and are specifically relevant to understanding cholesterol regulation, Alzheimer's disease and cystic fibrosis...
  10. AUTOMATED PROTEIN FOLD MODEL GENERATION
    Temple F Smith; Fiscal Year: 2003
    ..In addition, these libraries and their associated analysis tools will provide valuable data to aid in the experimental design to test predictions about the cellular roles of such proteins...
  11. Mechanism of the eukaryotic chaperonin TRiC/CCT
    Judith Frydman; Fiscal Year: 2007
    ..Characterize of the nucleotide cycle of the chaperonin TRiC; 2. Define the molecular basis of TRiC-substrate interactions; 3. Explore the mechanism of TRiC-assisted folding...
  12. RGS Protein Function in Cardiac Physiology
    Anthony J Muslin; Fiscal Year: 2007
    ..These experiments will help define the role of RGS proteins in the pathogenesis of cardiac hypertrophy and contractile dysfunction and may provide important information for the development of novel therapeutic agents...
  13. MAP Kinase and AKT Signaling in Congestive Heart Failure
    Anthony J Muslin; Fiscal Year: 2007
    ..Our findings will help to identify specific signaling mechanisms involved in the regulation of cardiac myocyte growth and metabolism...
  14. Protein Folding in the Eukaryotic Cytosol
    Judith Frydman; Fiscal Year: 2007
    ..Finally, our fourth specific aim will explore the interaction between chaperones and the translational machinery...
  15. Mechanism of the eukaryotic chaperonin TRiC/CCT
    Judith Frydman; Fiscal Year: 2008
    ..Define the molecular basis of TRiC-substrate interactions; 3. Explore the mechanism of TRiC-assisted folding. Public Health Relevance: This Public Health Relevance is not available...

Publications62

  1. Citrate, a possible precursor of astaxanthin in Phaffia rhodozyma: influence of varying levels of ammonium, phosphate and citrate in a chemically defined medium
    L B Flores Cotera
    Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF
    Appl Microbiol Biotechnol 55:341-7
    ..This suggested that restriction of protein synthesis could play an important role in carotenoid synthesis by P. rhodozyma...
  2. The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae
    F T Cooke
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    Curr Biol 8:1219-22
    ..Deletion of the FAB1 gene produces a loss of vacuolar morphology [6]; it is therefore concluded that PI(3,5)P2, the lipid product of Fab1p, is required for normal vacuolar function...
  3. Inversin, a novel gene in the vertebrate left-right axis pathway, is partially deleted in the inv mouse
    D Morgan
    Department of Human Genetics, University of Newcastle upon Tyne, UK
    Nat Genet 20:149-56
    ....
  4. Identification and functional analysis of hPRP17, the human homologue of the PRP17/CDC40 yeast gene involved in splicing and cell cycle control
    S Ben Yehuda
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel
    RNA 4:1304-12
    ..These results show the functional conservation of the Prp17 proteins in evolution and suggest that the second step of splicing takes place by a similar mechanism throughout eukaryotes...
  5. Pasteurella haemolytica complicated respiratory infections in sheep and goats
    K A Brogden
    Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, IA 50010, USA
    Vet Res 29:233-54
    ..This infection is detrimental to sheep and goats throughout the world and flocks and herds of small ranches, dairy operations, or large feedlots are all affected...
  6. Identification of a tRNA-specific nuclear export receptor
    U Kutay
    Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany
    Mol Cell 1:359-69
    ..RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm...
  7. Characterization of the maleylacetate reductase MacA of Rhodococcus opacus 1CP and evidence for the presence of an isofunctional enzyme
    V Seibert
    Institut für Mikrobiologie, Universität Stuttgart, D 70569 Stuttgart, Germany
    J Bacteriol 180:3503-8
    ..Thus, the R. opacus maleylacetate reductase gene macA clearly is not part of a specialized chlorocatechol gene cluster...
  8. Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans
    J F Staab
    Department of Medical Microbiology and Immunology, The Ohio State University College of Medicine, Columbus 43210 1239, USA
    Yeast 14:681-6
    ..The analyses suggested that Hwp1 is a glucan-linked protein with serine/threonine-rich regions that are predicted to function in extending a ligand-binding domain into the extracellular space...
  9. The crystal structure of phenol hydroxylase in complex with FAD and phenol provides evidence for a concerted conformational change in the enzyme and its cofactor during catalysis
    C Enroth
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Structure 6:605-17
    ..We propose that a movement of FAD takes place in concert with a large conformational change of residues 170-210 during catalysis...
  10. The lissencephaly gene product Lis1, a protein involved in neuronal migration, interacts with a nuclear movement protein, NudC
    S M Morris
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Biol 8:603-6
    ..The interaction of Lis1 with NudC, in conjunction with the MDS and ILS phenotypes, raises the possibility that nuclear movement in the ventricular zone is tied to the specification of neuronal fates and thus to cortical architecture...
  11. Characterisation of Bacillus stearothermophilus PcrA helicase: evidence against an active rolling mechanism
    L E Bird
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nucleic Acids Res 26:2686-93
    ..We propose that these data, together with existing structural evidence, support an inchworm rather than a rolling model for 3'-5' helicase activity...
  12. Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo
    D Z Rudner
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Mol Cell Biol 18:1765-73
    ..These data indicate that Drosophila U2AF heterodimer formation is essential for viability in vivo, consistent with a requirement for both subunits in splicing in vitro...
  13. Conservation in budding yeast of a kinase specific for SR splicing factors
    C W Siebel
    Department of Biochemistry and Biophysics, University of California, 513 Parnassus Avenue, San Francisco, CA 94143 0448, USA
    Proc Natl Acad Sci U S A 96:5440-5
    ..The unexpected discovery of an SR protein kinase in budding yeast provides a foundation for genetic dissection of the biological functions of SR proteins and their kinases...
  14. Clathrin functions in the absence of heterotetrameric adaptors and AP180-related proteins in yeast
    K M Huang
    Case Western Reserve University, Department of Molecular Biology and Microbiology, Cleveland, OH 44106 4960, USA
    EMBO J 18:3897-908
    ..Therefore, alternative mechanisms for clathrin assembly and coated vesicle formation, as well as the role of AP complexes and AP180-related proteins in these processes, must be considered...
  15. Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administration of diesel exhaust particles
    Y Tsurudome
    Department of Environmental Oncology and Department of Environmental Health Engineering, Institute of Industrial Ecological Sciences and Department of Biochemistry and Department of Surgery 1, School of Medicine, Onogawa, Tsukuba, Ibaraki 305 0053, Japan
    Carcinogenesis 20:1573-6
    ....
  16. Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins
    M Morita
    Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Japan
    Cell Struct Funct 25:309-15
    ..These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes...
  17. The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae
    M Radji
    Section of Animal Molecular Genetics, Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5 Takayama, Ikoma City, Nara, Japan, 630 0101
    Yeast 18:195-205
    ..We also found that the CDC39 gene was a multicopy suppressor of cdc50-1 mutation, suggesting that the CDC50 family is involved in regulation of transcription via CDC39...
  18. Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation
    P E Row
    Wellcome Trust Centre for the Study of Molecular Mechanisms in Disease, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 353:655-61
    ....
  19. Screening for modulators of spermine tolerance identifies Sky1, the SR protein kinase of Saccharomyces cerevisiae, as a regulator of polyamine transport and ion homeostasis
    O Erez
    Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 21:175-84
    ..While deletion or overexpression of SKY1 did not significantly affect Pma1p activity, the ability of overexpressed Sky1p, Ptk1p, and Ptk2p to increase sensitivity to LiCl depends on the integrity of PPZ1 but not of ENA1...
  20. Functional interaction between Ssu72 and the Rpb2 subunit of RNA polymerase II in Saccharomyces cerevisiae
    D L Pappas
    Department of Biochemistry, Division of Nucleic Acids Enzymology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Mol Cell Biol 20:8343-51
    ..These results define Ssu72 as a highly conserved factor that physically and functionally interacts with the RNAP II core machinery during transcription initiation...
  21. SGD1 encodes an essential nuclear protein of Saccharomyces cerevisiae that affects expression of the GPD1 gene for glycerol 3-phosphate dehydrogenase
    N Akhtar
    Emory University School of Medicine, Atlanta, Georgia, USA
    FEBS Lett 483:87-92
    ..Consistent with Sgd1p affecting the transcriptional control of GPD1, a functional green fluorescent protein tagged Sgd1p is localized to the cell nucleus...
  22. Primary structure, functional characteristics and tissue expression pattern of human ATA2, a subtype of amino acid transport system A
    T Hatanaka
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912 2100, USA
    Biochim Biophys Acta 1467:1-6
    ..The transporter is specific for neutral amino acids. It is pH-sensitive and Li+-intolerant. The Na+:amino acid stoichiometry is 1:1...
  23. Poly(A) tail-dependent exonuclease AtRrp41p from Arabidopsis thaliana rescues 5.8 S rRNA processing and mRNA decay defects of the yeast ski6 mutant and is found in an exosome-sized complex in plant and yeast cells
    J A Chekanova
    Department of Biological Sciences and the Center for Molecular Genetics, State University of New York at Albany, Albany, New York 12222, USA
    J Biol Chem 275:33158-66
    ....
  24. Overexpression of salicylate hydroxylase and the crucial role of lys(163) as its NADH binding site
    K Suzuki
    Department of Chemistry, Faculty of Science, Kanazawa University, Kakuma machi, Kanazawa 920 1192, Japan
    J Biochem 128:293-9
    ..Based on these results, Lys163 is proposed to play a role in the binding of NADH at the active site through an ionic bond rather than playing a role in catalysis...
  25. Identification of CYP4F8 in human seminal vesicles as a prominent 19-hydroxylase of prostaglandin endoperoxides
    J Bylund
    Division of Biochemical Pharmacology, Department of Pharmaceutical Biosciences, Uppsala Biomedical Centre, Uppsala University, SE 751 24 Uppsala, Sweden
    J Biol Chem 275:21844-9
    ..CYP4F8 is the first described hydroxylase with specificity and catalytic competence for prostaglandin endoperoxides...
  26. A new self-assembled peroxisomal vesicle required for efficient resealing of the plasma membrane
    G Jedd
    Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021 6399, USA
    Nat Cell Biol 2:226-31
    ..Our results show that the Woronin body represents a new category of peroxisome with a function in the maintenance of cellular integrity...
  27. Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake
    C W Yun
    Liver Diseases Section, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1800, USA
    J Biol Chem 275:10709-15
    ..These data indicate that S. cerevisiae has two pathways for ferrrioxamine-mediated iron uptake, one occurring at the plasma membrane and the other occurring in an intracellular compartment...
  28. IQGAP1 and calmodulin modulate E-cadherin function
    Z Li
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston Massachusetts 02115, USA
    J Biol Chem 274:37885-92
    ....
  29. The osmotic-1 locus of Neurospora crassa encodes a putative histidine kinase similar to osmosensors of bacteria and yeast
    M M Schumacher
    University of Colorado Health Sciences Center, B 111, 4200 East 9th Avenue, Denver, CO 80262, USA
    Curr Microbiol 34:340-7
    ..Our results indicate that nik-1 and os-1 encode the same osmosensing histidine kinase that plays an important role in the regulation of cell-wall assembly and, probably, other cell responses to changes in external osmolarity...
  30. The first structure of an aldehyde dehydrogenase reveals novel interactions between NAD and the Rossmann fold
    Z J Liu
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA
    Nat Struct Biol 4:317-26
    ..Sequence comparisons of the class 3 ALDH with other ALDHs indicate a similar polypeptide fold, novel NAD-binding mode and catalytic site for this family. A mechanism for enzymatic specificity and activity is postulated...
  31. SpoVH and spoVJ--new sporulation loci in Bacillus subtilis 168
    S H Hill
    J Gen Microbiol 129:293-302
    ..Recombination index values indicate that the mutations are in three separate genes. They define at least two new sporulation loci, designated spoVH and spoVJ...
  32. Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II
    C L Denis
    Genetics 108:833-44
    ..A model of ADHII regulation is proposed in which both ADR1 and CCR4 are required for ADHII expression. CRE1 and CRE2 negatively control CCR4, whereas CCR1 is required for ADR1 function...
  33. A mutation affecting lipoamide dehydrogenase, pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase activities in Saccharomyces cerevisiae
    J R Dickinson
    Mol Gen Genet 204:103-7
    ..Homozygous lpd1 diploids were unable to sporulate indicating that some lipoamide dehydrogenase activity is essential for sporulation to occur on acetate...
  34. The nucleotide sequence of the LPD1 gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae: comparison between eukaryotic and prokaryotic sequences for related enzymes and identification of potential upstream control sites
    J Ross
    Department of Microbiology, University of Edinburgh, UK
    J Gen Microbiol 134:1131-9
    ..The amino acid sequence also reveals the presence of a potential targeting sequence at its N-terminus which may facilitate transport to and entry into mitochondria...
  35. Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2)
    B Guiard
    EMBO J 4:3265-72
    ..This is the first reported mutation affecting the structural gene of cytochrome b2...
  36. cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen
    M Yaneva
    Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030
    J Biol Chem 264:13407-11
    ..The largest cDNA hybridized to 2.7- and 3.4-kilobase poly(A)+ mRNAs from HeLa cells. The cDNA clones expressed fusion proteins immunoreactive with the monoclonal antibody and sera from patients with autoimmune diseases...
  37. An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains
    W B Li
    Department of Microbiology, Dartmouth Medical School, Hanover, NH 03756
    Nucleic Acids Res 17:9621-36
    ....
  38. Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae
    F Klebl
    Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Federal Republic of Germany
    J Bacteriol 171:6259-64
    ..Haploid yeast cells contained one copy of the gene (BGL2). Gene disruption did not result in a phenotype...
  39. Cloning and characterization of an amidase gene from Rhodococcus species N-774 and its expression in Escherichia coli
    Y Hashimoto
    Department of Agricultural Chemistry, University of Tokyo, Japan
    Biochim Biophys Acta 1088:225-33
    ..Because the TAG translational stop codon of the amidase is located only 75 base pairs apart from the ATG start codon of the alpha-subunit of NHase, these genes are probably translated in a polycistronic manner...
  40. Amino acid alterations essential for increasing the catalytic activity of the nylon-oligomer-degradation enzyme of Flavobacterium sp
    K Kato
    Department of Fermentation Technology, Osaka University, Japan
    Eur J Biochem 200:165-9
    ..e. Gly181----Asp (EII type) and His266----Asn (EII type), enhanced the activity toward 6-aminohexanoate dimer 1000-fold...
  41. Calcium-sensitive cls mutants of Saccharomyces cerevisiae showing a Pet- phenotype are ascribable to defects of vacuolar membrane H(+)-ATPase activity
    Y Ohya
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 266:13971-7
    ....
  42. A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe
    H P Xu
    Cold Spring Harbor Laboratory, New York 11724
    Mol Biol Cell 3:721-34
    ..Expression of CNBP in yeast can partially suppress conjugation defects of cells lacking byr3...
  43. 2,3-Dihydroxybenzoic acid decarboxylase from Aspergillus niger. A novel decarboxylase
    R Santha
    Department of Biochemistry, Indian Institute of Science, Bangalore
    Eur J Biochem 230:104-10
    ..This is the first report on the active-site peptide and the partial primary structure of a non-oxidative decarboxylase catalyzing the removal of a carboxyl group from an aromatic nucleus...
  44. NudF, a nuclear migration gene in Aspergillus nidulans, is similar to the human LIS-1 gene required for neuronal migration
    X Xiang
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854 5635, USA
    Mol Biol Cell 6:297-310
    ..This strong similarity suggests that the LIS-1 gene product may have a function similar to that of NUDF and supports previous findings to suggest that nuclear migration may play a role in neuronal migration...
  45. Expression cloning of 2-5A-dependent RNAase: a uniquely regulated mediator of interferon action
    A Zhou
    Department of Cancer Biology, Cleveland Clinic Foundation, Ohio 44195
    Cell 72:753-65
    ..coli. Interestingly, a duplicated phosphate-binding loop motif was determined by deletion analysis and site-directed mutagenesis to function in the binding of 2-5A...
  46. bmr3, a third multidrug transporter gene of Bacillus subtilis
    R Ohki
    Department of Molecular Biology, School of Health Sciences, Kyorin University, Tokyo, Japan
    J Bacteriol 179:1423-7
    ..Although Bmr3 shows relatively low homology to Bmr and Blt, the substrate specificities of these three transporters overlap. Northern hybridization analysis showed that expression of the bmr3 gene was dependent on the growth phase...
  47. A unique origin and multistep process for the generation of expanded DRPLA triplet repeats
    H Yanagisawa
    National Children s Medical Research Center, Tokyo, Japan
    Hum Mol Genet 5:373-9
    ....
  48. Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins
    E Kim
    Howard Hughes Medical Institute, Department of Neurobiology, Massachusetts General Hospital, Boston 02114, USA
    Neuron 17:103-13
    ..Thus, chapsyn-110 and PSD-95 may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signalling proteins...
  49. Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA
    R Frothingham
    Infectious Diseases Section, Durham Veterans Affairs Medical Center, North Carolina 27705, USA
    Antimicrob Agents Chemother 40:1426-31
    ..coli cannot. We conclude that a single E. coli enzyme accounts for both PZAase and nicotinamidase activities and that ORF1 is the E.coli PZAase and nicotinamidase gene, pncA...
  50. Specific dephosphorylation of phosphopeptides by the yeast alkaline phosphatase encoded by PHO8 gene
    A Donella Deana
    Dipartimento di Chimica Biologica, Università di Padova, Italy
    Biochim Biophys Acta 1177:221-8
    ..This conclusion is consistent with the lack of homology with any class of known protein phosphatases...
  51. SPK1 is an essential S-phase-specific gene of Saccharomyces cerevisiae that encodes a nuclear serine/threonine/tyrosine kinase
    P Zheng
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510
    Mol Cell Biol 13:5829-42
    ....
  52. Isolation and characterization of a cDNA encoding rat liver cytosolic epoxide hydrolase and its functional expression in Escherichia coli
    M Knehr
    Institute of Toxicology, University of Mainz, Germany
    J Biol Chem 268:17623-7
    ..The isolated cDNA was expressed in bacteria to yield a catalytically active enzyme. Specific activity of the crude lysate obtained exceeded that of rat liver cytosols from maximally induced animals by a factor of 8...
  53. Characterization of al-2, the phytoene synthase gene of Neurospora crassa. Cloning, sequence analysis, and photoregulation
    T J Schmidhauser
    School of Medicine, Southern Illinois University, Carbondale 62901 4413
    J Biol Chem 269:12060-6
    ..crassa mycelia. The photoinduced increase in al-2 mRNA levels was not observed in two Neurospora mutants, wc-1 and wc-2, that are defective in all physiological photoresponses...
  54. Cloning and characterization of the PDA6-1 gene encoding a fungal cytochrome P-450 which detoxifies the phytoalexin pisatin from garden pea
    C Reimmann
    Department of Plant Pathology, University of Arizona, Tucson 85721
    Gene 146:221-6
    ..We conclude that PDA6-1, which does not appear to contribute to the virulence of N. haematococa on pea, differs significantly from PDAT9, which is associated with high virulence...
  55. Nitric oxide reductase cytochrome P-450 gene, CYP 55, of the fungus Fusarium oxysporum containing a potential binding-site for FNR, the transcription factor involved in the regulation of anaerobic growth of Escherichia coli
    D Tomura
    Institute of Applied Biochemistry, University of Tsukuba, Ibaraki
    J Biochem 116:88-94
    ..e., a combination of an FNR-like system and a system responding to nitrate/nitrite...
  56. Cloning, sequence analysis, and expression of the gene encoding formaldehyde dismutase from Pseudomonas putida F61
    H Yanase
    Department of Biotechnology, Faculty of Engineering, Tottori University, Japan
    Biosci Biotechnol Biochem 59:197-202
    ..The enzyme produced in E. coli catalyzed the dismutation of formaldehyde to form methanol and formic acid at the ratio of 1:1 in the absence of the exogenous electron acceptor, NAD(H)...
  57. Molecular genetics of the yeast vacuolar H(+)-ATPase
    Y Anraku
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Exp Biol 172:67-81
    ..This article summarizes currently available information on the VMA genes and the molecular biological functions of the VMA gene products...