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| mutSSummaryGene Symbol: mutS Description: methyl-directed mismatch repair protein Summary: MutS has weak ATPase activity and may be involved in recognizing the DNA mismatch. [More information is available at EcoGene: EG10625]. MutS is one of the components of the MutHLS complex. [More information is available at EcoCyc: EG10625]. Alias: ECK2728, JW2703, ant, fdv, plm Species: Publications Deciphering the mismatch recognition cycle in MutS and MSH2-MSH6 using normal-mode analysisShayantani Mukherjee Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, USA Biophys J 96:1707-20 Construction and characterization of different MutS fusion proteins as recognition elements of DNA chip for detection of DNA mutationsLi Jun Bi Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People s Republic of China Biosens Bioelectron 21:135-44 Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutantsAnetta Nowosielska Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA DNA Repair (Amst) 4:773-81 The DNA repair helicase UvrD is essential for replication fork reversal in replication mutantsMaria Jose Flores Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France EMBO Rep 5:983-8 ATP increases the affinity between MutS ATPase domains. Implications for ATP hydrolysis and conformational changesMeindert H Lamers Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam J Biol Chem 279:43879-85 Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approachHanjing Yang Department of Microbiology, Immunology and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, 405 Hilgard Avenue, University of California, Los Angeles, CA 90095, USA Mol Microbiol 53:283-95 Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutantsNina Costantino Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Building 539, PO Box B, Frederick, MD 21702 1201, USA Proc Natl Acad Sci U S A 100:15748-53 Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coliXin tian Li Department of Biochemistry, The University of Hong Kong, 3 F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, PR China Nucleic Acids Res 31:6674-87 What is driving the acquisition of mutS and rpoS polymorphisms in Escherichia coli?Thomas Ferenci School of Molecular and Microbial Biosciences G08, University of Sydney, 2006, NSW, Australia Trends Microbiol 11:457-61 The coordinated functions of the E. coli MutS and MutL proteins in mismatch repairSamir Acharya Genetics and Molecular Biology Program, Kimmel Cancer Center BLSB 933, 233 S 10th Street, Philadelphia, PA 19107, USA Mol Cell 12:233-46 | Scientific Experts
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Publications
Deciphering the mismatch recognition cycle in MutS and MSH2-MSH6 using normal-mode analysisShayantani Mukherjee
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, USA
Biophys J 96:1707-20
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, USA
Biophys J 96:1707-20
..The first step in mismatch repair is the recognition of base-base mismatches and insertions/deletions by bacterial MutS or eukaryotic MSH2-MSH6...
Construction and characterization of different MutS fusion proteins as recognition elements of DNA chip for detection of DNA mutationsLi Jun Bi
Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People s Republic of China
Biosens Bioelectron 21:135-44
Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People s Republic of China
Biosens Bioelectron 21:135-44
Three MutS fusion systems were designed as the mutation recognition and signal elements of DNA chips for detection of DNA mutations...
Cisplatin induces DNA double-strand break formation in Escherichia coli dam mutantsAnetta Nowosielska
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
DNA Repair (Amst) 4:773-81
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
DNA Repair (Amst) 4:773-81
Escherichia coli dam cells are more susceptible to the cytotoxic action of cisplatin than wildtype. Dam mutS or dam mutL bacteria, however, are resistant to this agent indicating that active mismatch repair sensitizes dam cells to ..
The DNA repair helicase UvrD is essential for replication fork reversal in replication mutantsMaria Jose Flores
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France
EMBO Rep 5:983-8
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France
EMBO Rep 5:983-8
..coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli...
ATP increases the affinity between MutS ATPase domains. Implications for ATP hydrolysis and conformational changesMeindert H Lamers
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam
J Biol Chem 279:43879-85
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam
J Biol Chem 279:43879-85
b>MutS is the key protein of the Escherichia coli DNA mismatch repair system. It recognizes mispaired and unpaired bases and has intrinsic ATPase activity...
Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approachHanjing Yang
Department of Microbiology, Immunology and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, 405 Hilgard Avenue, University of California, Los Angeles, CA 90095, USA
Mol Microbiol 53:283-95
Department of Microbiology, Immunology and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, 405 Hilgard Avenue, University of California, Los Angeles, CA 90095, USA
Mol Microbiol 53:283-95
..genes include those encoding DNA helicases (UvrD, RecG, RecQ), truncated forms of the DNA mismatch repair protein (MutS) and a primosomal component (DnaT), a negative modulator of initiation of replication/GATC-binding protein (SeqA), ..
Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutantsNina Costantino
Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Building 539, PO Box B, Frederick, MD 21702 1201, USA
Proc Natl Acad Sci U S A 100:15748-53
Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Building 539, PO Box B, Frederick, MD 21702 1201, USA
Proc Natl Acad Sci U S A 100:15748-53
..Our results show that Beta is the only bacteriophage function required for this level of recombination and suggest that Beta directs the ssDNA to the replication fork as it passes the target sequence...
Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coliXin tian Li
Department of Biochemistry, The University of Hong Kong, 3 F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, PR China
Nucleic Acids Res 31:6674-87
Department of Biochemistry, The University of Hong Kong, 3 F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, PR China
Nucleic Acids Res 31:6674-87
..This reflects the fact that different base-base mispairs are corrected by the mutS/H/L-dependent MMR pathway with differing efficiencies...
What is driving the acquisition of mutS and rpoS polymorphisms in Escherichia coli?Thomas Ferenci
School of Molecular and Microbial Biosciences G08, University of Sydney, 2006, NSW, Australia
Trends Microbiol 11:457-61
School of Molecular and Microbial Biosciences G08, University of Sydney, 2006, NSW, Australia
Trends Microbiol 11:457-61
Pathogenic and commensal Escherichia coli isolates frequently contain defective alleles of the mutS and rpoS genes, located in a highly polymorphic segment of the chromosome...
The coordinated functions of the E. coli MutS and MutL proteins in mismatch repairSamir Acharya
Genetics and Molecular Biology Program, Kimmel Cancer Center BLSB 933, 233 S 10th Street, Philadelphia, PA 19107, USA
Mol Cell 12:233-46
Genetics and Molecular Biology Program, Kimmel Cancer Center BLSB 933, 233 S 10th Street, Philadelphia, PA 19107, USA
Mol Cell 12:233-46
The Escherichia coli MutS and MutL proteins have been conserved throughout evolution, although their combined functions in mismatch repair (MMR) are poorly understood...
Competition between MutY and mismatch repair at A x C mispairs In vivoMandy Kim
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, USA
J Bacteriol 185:4626-9
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, USA
J Bacteriol 185:4626-9
We show that the MutY protein competes with the MutS-dependent mismatch repair system to process at least some A. C mispairs in vivo, converting them to G. C pairs...
Molecular analysis of mutS expression and mutation in natural isolates of pathogenic Escherichia coliBaoguang Li
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA
Microbiology 149:1323-31
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA
Microbiology 149:1323-31
Deficiencies in the MutS protein disrupt methyl-directed mismatch repair (MMR), generating a mutator phenotype typified by high mutation rates and promiscuous recombination...
Conjugational hyperrecombination achieved by derepressing the LexA regulon, altering the properties of RecA protein and inactivating mismatch repair in Escherichia coli K-12Vladislav A Lanzov
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721 0106, USA
Genetics 163:1243-54
Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721 0106, USA
Genetics 163:1243-54
..We discuss mechanisms by which the lexA, recA, and mutS mutations may elevate FRE cumulatively to obtain hyperrecombination.
MutS inhibits RecA-mediated strand transfer with methylated DNA substratesMelissa A Calmann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School Worcester, MA 01605, USA
Nucleic Acids Res 33:3591-7
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School Worcester, MA 01605, USA
Nucleic Acids Res 33:3591-7
..In DNA methylated by MNNG, MMR action is the result of MutS recognition of O6-methylguanine base pairs...
The MutS C terminus is essential for mismatch repair activity in vivoMelissa A Calmann
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
J Bacteriol 187:6577-9
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
J Bacteriol 187:6577-9
An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutSdelta800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure...
Mutations in the signature motif in MutS affect ATP-induced clamp formation and mismatch repairSamir Acharya
Department of Molecular Virology, Immunology and Medical Genetics, and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA
Mol Microbiol 69:1544-59
Department of Molecular Virology, Immunology and Medical Genetics, and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA
Mol Microbiol 69:1544-59
SUMMARY: MutS protein dimer recognizes and co-ordinates repair of DNA mismatches...
Steady-state ATPase activity of E. coli MutS modulated by its dissociation from heteroduplex DNASeong Dal Heo
Department of Chemistry, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, Gyungbuk 790 784, Republic of Korea
Biochem Biophys Res Commun 364:264-9
Department of Chemistry, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, Gyungbuk 790 784, Republic of Korea
Biochem Biophys Res Commun 364:264-9
The ability of MutS to recognize mismatched DNA is required to initiate a mismatch repair (MMR) system. ATP binding and hydrolysis are essential in this process, but their role in MMR is still not fully understood...
Roles of RecA protein in spontaneous mutagenesis in Escherichia coliSatoshi Kanie
Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan
Genes Genet Syst 82:99-108
Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan
Genes Genet Syst 82:99-108
..the single-base frameshifts induced in the recA-deficient cells appeared to be efficiently suppressed by the mutS-dependent mismatch repair system...
Role of the 5' --> 3' exonuclease and Klenow fragment of Escherichia coli DNA polymerase I in base mismatch repairMasaru Imai
Graduate School of Life Sciences, Tohoku University, Sendai, 980 8577, Japan
Mol Genet Genomics 278:211-20
Graduate School of Life Sciences, Tohoku University, Sendai, 980 8577, Japan
Mol Genet Genomics 278:211-20
We have previously demonstrated that the Escherichia coli strain mutS DeltapolA had a higher rate of transition and minus frameshift mutations than mutS or DeltapolA strains...
Escherichia coli MutS tetramerization domain structure reveals that stable dimers but not tetramers are essential for DNA mismatch repair in vivoMarc L Mendillo
Ludwig Institute for Cancer Research, Department of Medicine, University of California, San Diego School of Medicine, La Jolla, California 92093 0669, USA
J Biol Chem 282:16345-54
Ludwig Institute for Cancer Research, Department of Medicine, University of California, San Diego School of Medicine, La Jolla, California 92093 0669, USA
J Biol Chem 282:16345-54
The Escherichia coli mispair-binding protein MutS forms dimers and tetramers in vitro, although the functional form in vivo is under debate...
Modulation of MutS ATP-dependent functional activities by DNA containing a cisplatin compound lesion (base damage and mismatch)Yuliya Sedletska
Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans Cedex 02, France
J Mol Biol 369:27-40
Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans Cedex 02, France
J Mol Biol 369:27-40
..The present study investigated whether ATP-dependent biochemical properties of Escherichia coli MutS protein differ when the protein interacts with a DNA oligonucleotide containing a GT mismatch versus a unique site ..
Physical and functional interactions between Escherichia coli MutY glycosylase and mismatch repair protein MutSHaibo Bai
Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
J Bacteriol 189:902-10
Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201, USA
J Bacteriol 189:902-10
Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C...
Structural and functional analysis of the MutS C-terminal tetramerization domainLaura Manelyte
Institut für Biochemie, Justus Liebig Universität, Heinrich Buff Ring 58, D 35392 Giessen, Germany
Nucleic Acids Res 34:5270-9
Institut für Biochemie, Justus Liebig Universität, Heinrich Buff Ring 58, D 35392 Giessen, Germany
Nucleic Acids Res 34:5270-9
The Escherichia coli DNA mismatch repair (MMR) protein MutS is essential for the correction of DNA replication errors...
Electrochemical detection of mismatched DNA using a MutS probeMinseon Cho
Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk, 790 784, South Korea
Nucleic Acids Res 34:e75
Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk, 790 784, South Korea
Nucleic Acids Res 34:e75
..b>MutS on a Ni-NTA coated Au electrode...
A MutS protein-immobilized au electrode for detecting single-base mismatch of DNAAishan Han
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan
Anal Sci 22:663-6
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan
Anal Sci 22:663-6
A novel electrochemical biosensor was developed to detect gene mutation by using a DNA-mismatch binding protein: MutS from Escherichia coli...
Dual role of MutS glutamate 38 in DNA mismatch discrimination and in the authorization of repairJoyce H G Lebbink
Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, The Netherlands
EMBO J 25:409-19
Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, The Netherlands
EMBO J 25:409-19
b>MutS plays a critical role in DNA mismatch repair in Escherichia coli by binding to mismatches and initiating repair in an ATP-dependent manner...
Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coliJennifer L Robbins Manke
Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Ave, 56 670, Cambridge, MA 02139, USA
J Bacteriol 187:7027-37
Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Ave, 56 670, Cambridge, MA 02139, USA
J Bacteriol 187:7027-37
..coli strains deficient in Dam and mismatch repair (dam, dam mutS, and mutS mutants)...
Binding discrimination of MutS to a set of lesions and compound lesions (base damage and mismatch) reveals its potential role as a cisplatin-damaged DNA sensing proteinLaurence Fourrier
Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans Cedex 02, France
J Biol Chem 278:21267-75
Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans Cedex 02, France
J Biol Chem 278:21267-75
..shift competition assays and surface plasmon resonance, we have analyzed the interaction of Escherichia coli MutS protein with site-specifically modified DNA oligonucleotides containing each of the four cisplatin cross-links or a ..
The Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesionsJennifer Wyrzykowski
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Bacteriol 185:1701-4
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Bacteriol 185:1701-4
..These findings demonstrate that MMR has the ability to prevent oxidative mutagenesis either by removing 8-oxoG directly or by removing adenine misincorporated opposite 8-oxoG or both...
Interaction of Escherichia coli MutS and MutL at a DNA mismatchM J Schofield
Genetics and Biochemistry Branch, NIDDKD, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:28291-9
Genetics and Biochemistry Branch, NIDDKD, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:28291-9
b>MutS and MutL are both required to activate downstream events in DNA mismatch repair...
Reduction of GC --> TA transversion mutation by overexpression of MutS in Escherichia coli K-12J Zhao
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
J Bacteriol 182:5025-8
Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
J Bacteriol 182:5025-8
Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC --> TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-..
Antagonism of ultraviolet-light mutagenesis by the methyl-directed mismatch-repair system of Escherichia coliH Liu
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon 97331 7301, USA
Genetics 154:503-12
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon 97331 7301, USA
Genetics 154:503-12
..F' lacZ targets were mated from mut(+) donors into mutH, mutL, or mutS recipients, once cells were at substantial densities, to minimize spontaneous mutation prior to irradiation...
ATP-hydrolysis-dependent conformational switch modulates the stability of MutS-mismatch complexesA Joshi
Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay 400005, India
Nucleic Acids Res 28:853-61
Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay 400005, India
Nucleic Acids Res 28:853-61
..Here we demonstrate that nucleotide (ADP or ATP) binding to MutS mediates a switch in protein conformation...
ATP hydrolysis-dependent formation of a dynamic ternary nucleoprotein complex with MutS and MutLL Galio
Genoscope, Centre National de Séquençage, BP 191, 2 rue Gaston Crémieux, 91006 Evry, France
Nucleic Acids Res 27:2325-31
Genoscope, Centre National de Séquençage, BP 191, 2 rue Gaston Crémieux, 91006 Evry, France
Nucleic Acids Res 27:2325-31
Functional interactions of Escherichia coli MutS and MutL in mismatch repair are dependent on ATP...
High mutation frequencies among Escherichia coli and Salmonella pathogensJ E LeClerc
Molecular Biology Branch, Center for Food Safety and Applied Nutrition HFS 235, Food and Drug Administration, Washington, DC 20204, USA
Science 274:1208-11
Molecular Biology Branch, Center for Food Safety and Applied Nutrition HFS 235, Food and Drug Administration, Washington, DC 20204, USA
Science 274:1208-11
..Of nine independently derived hypermutable strains, seven contained a defective mutS allele...
Multicopy single-stranded DNA of Escherichia coli enhances mutation and recombination frequencies by titrating MutS proteinW K Maas
Department of Microbiology, New York University Medical Center, New York USA
Mol Microbiol 19:505-9
Department of Microbiology, New York University Medical Center, New York USA
Mol Microbiol 19:505-9
..interference with mismatch repair by msDNA, we show that the addition of a plasmid containing the gene for MutS protein suppresses the mutagenic and recombinogenic effects of msDNAs...
The Escherichia coli MutS DNA mismatch binding protein specifically binds O(6)-methylguanine DNA lesionsL J Rasmussen
Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA 02115, USA
Carcinogenesis 17:2085-8
Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA 02115, USA
Carcinogenesis 17:2085-8
..Here we show that the Escherichia coli MutS DNA mismatch repair binding protein does indeed bind specifically to O6MeG DNA lesions...
Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12G Feng
University of Texas, Houston Medical School, USA
Biotechniques 19:956-65
University of Texas, Houston Medical School, USA
Biotechniques 19:956-65
The MutS, MutH and MutL proteins mediate methyl-directed-mismatch (MDM) repair in Escherichia coli and Salmonella typhimurium...
Anaerobiosis induces expression of ant, a new Escherichia coli locus with a role in anaerobic electron transportJ H Yerkes
J Bacteriol 158:180-6
J Bacteriol 158:180-6
..The Mu d1(Ap lac) insertions in these mutants were genetically mapped between mutS and srl and thus define a new locus we have termed ant (anaerobic electron transport)...
Selection of a mutant of Escherichia coli which has high mutation ratesR B Helling
J Bacteriol 96:975-80
J Bacteriol 96:975-80
..This mutation (mut-2) and the Treffers' mutation (mutT1) mapped at approximately the same position to the right of leu. Specificity studies showed that the two mutations differ in rates of mutation produced...
Cloning of hydrogenase genes and fine structure analysis of an operon essential for H2 metabolism in Escherichia coliP Sankar
J Bacteriol 162:353-60
J Bacteriol 162:353-60
..also established the presence of two new genes, so far unidentified, one coding for formate dehydrogenase-2 (fdv) and another producing an electron transport protein (fhl) coupling formate dehydrogenase-2 to hydrogenase...
The Escherichia coli fdv gene probably encodes mutS and is located at minute 58.8 adjacent to the hyc-hyp gene clusterV Schlensog
Lehrstuhl für Mikrobiologie, Universität München, Germany
J Bacteriol 173:7414-5
Lehrstuhl für Mikrobiologie, Universität München, Germany
J Bacteriol 173:7414-5
In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repairV Burdett
Department of Biochemistry and Howard Hughes Medical Institute, Box 3711, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 98:6765-70
Department of Biochemistry and Howard Hughes Medical Institute, Box 3711, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 98:6765-70
..phenotypes associated with 2-aminopurine exposure are effectively suppressed by null mutations in mutH, mutL, mutS, or uvrD/mutU, which encode activities that act upstream of the four exonucleases in the mechanism for the methyl-..
Redundant exonuclease involvement in Escherichia coli methyl-directed mismatch repairM Viswanathan
Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254 9110, USA
J Biol Chem 276:31053-8
Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254 9110, USA
J Biol Chem 276:31053-8
..This elevation is epistatic to deficiency for MutS, suggesting an effect via the mismatch repair pathway. Our other work (Burdett, V., Baitinger, C., Viswanathan, M...
Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysisL J Blackwell
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 276:34339-47
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 276:34339-47
The role of MutS ATPase in mismatch repair is controversial. To clarify further the function of this activity, we have examined adenine nucleotide effects on interactions of Escherichia coli MutS with homoduplex and heteroduplex DNAs...
The alternating ATPase domains of MutS control DNA mismatch repairMeindert H Lamers
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
EMBO J 22:746-56
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
EMBO J 22:746-56
..In Escherichia coli, the MutS enzyme recognizes mismatches and initiates repair...
Stabilization of perfect and imperfect tandem repeats by single-strand DNA exonucleasesVladimir V Feschenko
Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 9110, USA
Proc Natl Acad Sci U S A 100:1134-9
Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454 9110, USA
Proc Natl Acad Sci U S A 100:1134-9
..analysis, we show that the inhibitory effect of ssDNA exonucleases on deletion formation is independent of the MutS and UvrD proteins...
An efficient method for the preparation of long heteroduplex DNA as substrate for mismatch repair by the Escherichia coli MutHLS systemEvangelos Thomas
Institut für Biochemie, Justus Liebig Universität, Giessen, Germany
Biol Chem 383:1459-62
Institut für Biochemie, Justus Liebig Universität, Giessen, Germany
Biol Chem 383:1459-62
..The application of this procedure is demonstrated for the analysis of the Escherichia coli MutHLS system...
Escherichia coli strains (ndk) lacking nucleoside diphosphate kinase are powerful mutators for base substitutions and frameshifts in mismatch-repair-deficient strainsJeffrey H Miller
Department of Microbiology, Immunology, and Molecular Genetics and The Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
Genetics 162:5-13
Department of Microbiology, Immunology, and Molecular Genetics and The Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
Genetics 162:5-13
..Double mutants that are ndk mutS, lacking both the NDP kinase and mismatch repair, have levels of base substitutions 15-fold higher and levels of ..
Saturation of DNA mismatch repair and error catastrophe by a base analogue in Escherichia coliKazuo Negishi
Gene Research Center, Okayama University, Okayama 700 8530, Japan
Genetics 161:1363-71
Gene Research Center, Okayama University, Okayama 700 8530, Japan
Genetics 161:1363-71
..At a low dose, dP is much more mutagenic in mismatch-repair-defective mutH, mutL, and mutS strains than in a wild-type strain...
ATP hydrolysis induces expansion of MutS contacts on heteroduplex: a case for MutS treadmilling?Amita Joshi
Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay 400 005, India
Biochemistry 41:3654-66
Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay 400 005, India
Biochemistry 41:3654-66
An unsolved problem in E. coli mismatch repair is how the MutS-MutL complex communicates positional information of a mismatch to MutH...
MutS recognition: multiple mismatches and sequence context effectsA Joshi
Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India
J Biosci 26:595-606
Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India
J Biosci 26:595-606
Escherichia coli MutS is a versatile repair protein that specifically recognizes not only various types of mismatches but also single stranded loops of up to 4 nucleotides in length...
MutS preferentially recognizes cisplatin- over oxaliplatin-modified DNAZoran Z Zdraveski
Department of Chemistry and Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 277:1255-60
Department of Chemistry and Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 277:1255-60
..This study compared the interactions of the purified Escherichia coli mismatch repair protein MutS with DNA modified to contain cisplatin and DACH adducts...
The Phe-X-Glu DNA binding motif of MutS. The role of hydrogen bonding in mismatch recognitionM J Schofield
Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:45505-8
Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:45505-8
The crystal structures of MutS protein from Thermus aquaticus and Escherichia coli in a complex with a mismatch-containing DNA duplex reveal that the Glu residue in a conserved Phe-X-Glu motif participates in a hydrogen-bonded contact ..
Interaction of MutS and Vsr: some dominant-negative mutS mutations that disable methyladenine-directed mismatch repair are active in very-short-patch repairM Lieb
Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
J Bacteriol 183:6487-90
Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
J Bacteriol 183:6487-90
..The products of mutS and mutL, which are required for adenine methylation-directed mismatch repair (MMR), enhance VSP repair...
Characterization of functional interactions among the Escherichia coli mismatch repair proteins using a bacterial two-hybrid assayC A Mansour
Biology Department, Concordia University, Montréal, Que, Canada
Mutat Res 485:331-8
Biology Department, Concordia University, Montréal, Que, Canada
Mutat Res 485:331-8
..b>MutS and MutL, part of the post-replication mismatch repair system, stimulate VSP repair...
A universal protein-protein interaction motif in the eubacterial DNA replication and repair systemsB P Dalrymple
Commonwealth Scientific and Industrial Research Organisation Livestock Industries, 120 Meiers Road, Indooroopilly QLD 4068, Australia
Proc Natl Acad Sci U S A 98:11627-32
Commonwealth Scientific and Industrial Research Organisation Livestock Industries, 120 Meiers Road, Indooroopilly QLD 4068, Australia
Proc Natl Acad Sci U S A 98:11627-32
..Comparison of the eubacterial, eukaryotic, and archaeal sliding clamp binding motifs suggests that the basic interactions have been conserved across the evolutionary landscape...
Initiation of methyl-directed mismatch repairK G Au
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
J Biol Chem 267:12142-8
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
J Biol Chem 267:12142-8
..1987) J. Biol. Chem. 262, 15624-15629). MutH endonuclease is activated in a reaction that requires MutS, MutL, ATP, and Mg2+ and depends upon the presence of a mismatch within the DNA...