Patricia A Zuk

Summary

Publications

  1. ncbi request reprint Comparison of multi-lineage cells from human adipose tissue and bone marrow
    Daniel A De Ugarte
    Departments of Surgery and Orthopaedics, UCLA School of Medicine, Los Angeles, California, USA
    Cells Tissues Organs 174:101-9. 2003
  2. doi request reprint Comparison of human mesenchymal stem cells derived from adipose tissue and bone marrow for ex vivo gene therapy in rat spinal fusion model
    Masashi Miyazaki
    Department of Orthopaedic Surgery, University of California at Los Angeles, Los Angeles, California, USA
    Spine (Phila Pa 1976) 33:863-9. 2008
  3. doi request reprint The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used
    Patricia A Zuk
    Regenerative Bioengineering and Repair Laboratory, Division of Plastic Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Cell Biochem 106:867-77. 2009
  4. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
  5. doi request reprint Tissue engineering craniofacial defects with adult stem cells? Are we ready yet?
    Patricia A Zuk
    Department of Surgery, David Geffen School of Medicine, The University of California at Los Angeles, Los Angeles, CA 10833, USA
    Pediatr Res 63:478-86. 2008
  6. ncbi request reprint Tissue-engineered bone from BMP-2-transduced stem cells derived from human fat
    Jason L Dragoo
    Department of Orthopedic Surgery, Laboratory for Regenerative Bioengineering and Repair, University of California, Los Angeles, California, USA
    Plast Reconstr Surg 115:1665-73. 2005
  7. ncbi request reprint Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow
    Daniel A De Ugarte
    Department of Surgery, UCLA School of Medicine, Regenerative Bioengineering and Repair Laboratory, Los Angeles, CA, USA
    Immunol Lett 89:267-70. 2003
  8. doi request reprint Human adipose stem cells: a potential cell source for cardiovascular tissue engineering
    Sepideh Heydarkhan-Hagvall
    Regenerative Bioengineering and Repair Laboratory, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, Calif, USA
    Cells Tissues Organs 187:263-74. 2008
  9. ncbi request reprint Chondrogenic potential of multipotential cells from human adipose tissue
    Jerry I Huang
    Department of Surgery, University of California, Los Angeles School of Medicine, 90095 6902, USA
    Plast Reconstr Surg 113:585-94. 2004
  10. ncbi request reprint In vitro differentiation of human processed lipoaspirate cells into early neural progenitors
    Peter H Ashjian
    Laboratory for Regenerative Bioengineering and Repair, Department of Surgery, University of California, Los Angeles, Calif 90095, USA
    Plast Reconstr Surg 111:1922-31. 2003

Research Grants

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Comparison of multi-lineage cells from human adipose tissue and bone marrow
    Daniel A De Ugarte
    Departments of Surgery and Orthopaedics, UCLA School of Medicine, Los Angeles, California, USA
    Cells Tissues Organs 174:101-9. 2003
    ..Adipose tissue is an abundant and easily procured source of PLA cells, which have a potential like MSCs for use in tissue-engineering applications and as gene delivery vehicles...
  2. doi request reprint Comparison of human mesenchymal stem cells derived from adipose tissue and bone marrow for ex vivo gene therapy in rat spinal fusion model
    Masashi Miyazaki
    Department of Orthopaedic Surgery, University of California at Los Angeles, Los Angeles, California, USA
    Spine (Phila Pa 1976) 33:863-9. 2008
    ..Rat spinal fusion model...
  3. doi request reprint The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used
    Patricia A Zuk
    Regenerative Bioengineering and Repair Laboratory, Division of Plastic Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Cell Biochem 106:867-77. 2009
    ..Therefore, confirming the expression of OCT4, Sox2, and Nanog in adult stem cells with today's commercial antibodies must be carefully considered before the designation of pluripotent can be granted...
  4. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
    ..Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression...
  5. doi request reprint Tissue engineering craniofacial defects with adult stem cells? Are we ready yet?
    Patricia A Zuk
    Department of Surgery, David Geffen School of Medicine, The University of California at Los Angeles, Los Angeles, CA 10833, USA
    Pediatr Res 63:478-86. 2008
    ..Finally, future directions with regards to the use of ASCs in craniofacial repair are discussed, including possible scaffold-driven and gene-driven approaches...
  6. ncbi request reprint Tissue-engineered bone from BMP-2-transduced stem cells derived from human fat
    Jason L Dragoo
    Department of Orthopedic Surgery, Laboratory for Regenerative Bioengineering and Repair, University of California, Los Angeles, California, USA
    Plast Reconstr Surg 115:1665-73. 2005
    ..The purpose of this study was to examine the in vivo bone induction capacity of bone morphogenetic protein-2 (BMP-2)-transduced processed lipoaspirate cells using adipose tissue from multiple harvest sites...
  7. ncbi request reprint Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow
    Daniel A De Ugarte
    Department of Surgery, UCLA School of Medicine, Regenerative Bioengineering and Repair Laboratory, Los Angeles, CA, USA
    Immunol Lett 89:267-70. 2003
    ..While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation...
  8. doi request reprint Human adipose stem cells: a potential cell source for cardiovascular tissue engineering
    Sepideh Heydarkhan-Hagvall
    Regenerative Bioengineering and Repair Laboratory, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, Calif, USA
    Cells Tissues Organs 187:263-74. 2008
    ..The objective of this study was to identify the exogenous and endogenous parameters that are critical for the differentiation of human adipose stem cells (hASCs) into cardiovascular cells...
  9. ncbi request reprint Chondrogenic potential of multipotential cells from human adipose tissue
    Jerry I Huang
    Department of Surgery, University of California, Los Angeles School of Medicine, 90095 6902, USA
    Plast Reconstr Surg 113:585-94. 2004
    ..In summary, human adipose tissue may represent a novel plentiful source of multipotential stem cells capable of undergoing chondrogenesis in vitro...
  10. ncbi request reprint In vitro differentiation of human processed lipoaspirate cells into early neural progenitors
    Peter H Ashjian
    Laboratory for Regenerative Bioengineering and Repair, Department of Surgery, University of California, Los Angeles, Calif 90095, USA
    Plast Reconstr Surg 111:1922-31. 2003
    ..The authors concluded that human PLA cells might have the potential to differentiate in vitro into cells that represent early progenitors of neurons and/or glia...
  11. ncbi request reprint In vitro microdistraction of preosteoblasts: distraction promotes proliferation and oscillation promotes differentiation
    Joubin S Gabbay
    Regenerative Bioengineering and Repair Lab, Division of Plastic and Reconstructive Surgery, David Geffen School of Medicine at University of California, Los Angeles, Calif, USA
    Tissue Eng 12:3055-65. 2006
    ..Therefore, these results suggest that the microdistraction system may be an appropriate in vitro system for the study of mechanobiology in osteoblast phenotype...
  12. doi request reprint BMP-2 does not influence the osteogenic fate of human adipose-derived stem cells
    Navanjun S Grewal
    Division of Plastic and Reconstructive Surgery, David Geffen School of Medicine at University of California, Los Angeles, CA, USA
    Plast Reconstr Surg 123:158S-65S. 2009
    ..However, whether BMP-2 induces an osteogenic response in the adipose-derived stem cells remains unknown...
  13. ncbi request reprint Myogenic differentiation by human processed lipoaspirate cells
    Hiroshi Mizuno
    Laboratory for Regenerative Bioengineering and Repair, Department of Surgery, University of California Los Angeles School of Medicine, 90095 1665, USA
    Plast Reconstr Surg 109:199-209; discussion 210-1. 2002
    ..In summary, our findings suggest that human processed lipoaspirate cells differentiate into myogenic cells. Furthermore, these cells may be a useful source for skeletal muscle engineering and repair...
  14. ncbi request reprint Bone induction by BMP-2 transduced stem cells derived from human fat
    Jason L Dragoo
    Laboratory for Regenerative Bioengineering and Repair, Department of Surgery, UCLA School of Medicine, 90095 6092, USA
    J Orthop Res 21:622-9. 2003
    ..This study examines the osteogenic potential of PLAs and bone marrow aspirate cells (BMAs), when exposed to either recombinant human bone morphogenetic protein (BMP)-2 (rh-BMP-2) or adenovirus containing BMP-2 cDNA (Ad-BMP-2)...
  15. doi request reprint Adipose-derived stem cells and BMP2: part 1. BMP2-treated adipose-derived stem cells do not improve repair of segmental femoral defects
    Yu Fen Chou
    Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, California, USA
    Connect Tissue Res 52:109-18. 2011
    ..Based on these results, the addition of ASCs to rhBMP2-treated scaffolds may provide no significant advantage in terms of the ability to heal bone...
  16. ncbi request reprint Downregulation of extracellular matrix-related gene clusters during osteogenic differentiation of human bone marrow- and adipose tissue-derived stromal cells
    Hiroshi Egusa
    Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA, USA
    Tissue Eng 13:2589-600. 2007
    ..This change may serve to normalize gene expression in mixed populations of stem cells derived from different tissues...
  17. ncbi request reprint Osteogenic potentiation of human adipose-derived stem cells in a 3-dimensional matrix
    Joubin S Gabbay
    Division of Plastic and Reconstructive Surgery, University of California, Los Angeles, 90095, USA
    Ann Plast Surg 57:89-93. 2006
    ..05], and 2.5-fold greater expression of JNK2 [P < 0.05]). Our data showed there was a progressive stimulatory effect on ADSCs with regard to osteogenesis when cultured in a 3-dimensional gel compared with a 2-D monolayer...
  18. ncbi request reprint Processed lipoaspirate cells for tissue engineering of the lower urinary tract: implications for the treatment of stress urinary incontinence and bladder reconstruction
    Gregory S Jack
    Department of Urology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
    J Urol 174:2041-5. 2005
    ..We performed a pilot study to investigate the ability of human adipose derived, multipotent stem cells to be delivered to and survive within bladder and urethral smooth muscle...
  19. pmc The adipose-derived stem cell: looking back and looking ahead
    Patricia A Zuk
    Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Mol Biol Cell 21:1783-7. 2010
    ..In this retrospective article, a historical perspective on the ASC is given together with exciting new applications for the stem cell being considered today...
  20. pmc Osteoblast interactions within a biomimetic apatite microenvironment
    Eric J Tsang
    Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, 5121 Engineering V, Los Angeles, CA 90095, USA
    Ann Biomed Eng 39:1186-200. 2011
    ..Therefore, the biomimetic apatite surface may significantly alter the microenvironment of adherent osteoblasts and, as such, be capable of affecting both cell survival and differentiation...
  21. doi request reprint Stem cells from human fat as cellular delivery vehicles in an athymic rat posterolateral spine fusion model
    Wellington K Hsu
    K4 703, 600 Highland Avenue, Box 7375 Clinical Science Center H4, Madison, University of Wisconsin, WI 53792 3284, USA
    J Bone Joint Surg Am 90:1043-52. 2008
    ..In this study, we sought to evaluate the efficacy of bone morphogenetic protein (BMP)-2-producing adipose-derived stem cells in inducing a posterolateral spine fusion in an athymic rat model...
  22. doi request reprint Freezing adipose tissue grafts may damage their ability to integrate into the host
    Navanjun Grewal
    Department of Surgery, Division of Plastic Surgery, Regenerative Bioengineering and Repair Laboratory, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Connect Tissue Res 50:14-28. 2009
    ..Specifically, it now appears that the practice of freezing in saline may not be advisable if graft viability, activity, and structure are to be maintained in vivo...
  23. doi request reprint Viral transduction of adipose-derived stem cells
    Patricia A Zuk
    Regenerative Bioengineering and Repair Laboratory, Division of Plastic Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    Methods Mol Biol 702:345-57. 2011
    ..As such, this chapter describes methods for the transduction of human ASCs with two popular types of recombinant viruses: adenovirus and lentivirus...
  24. ncbi request reprint The role of cytoprotective cytokines in cardiac ischemia/reperfusion injury
    Corey D Anderson
    Department of Surgery, Cardiothoracic Surgery Division, David Geffen School of Medicine, University of California, Los Angeles, California, USA
    J Surg Res 148:164-71. 2008
    ..IFS was decreased 4.2-fold in the HGF treated group compared with the PBS control group. Thus, HGF acutely post-MI using polymer delivery reduces IFS, leading to beneficial effects on post-MI LV remodeling...
  25. ncbi request reprint Healing full-thickness cartilage defects using adipose-derived stem cells
    Jason L Dragoo
    Orthopedic Tissue Regeneration Laboratory, Department of Orthopedic Surgery, Stanford University School of Medicine, Palo Alto, California 94304, USA
    Tissue Eng 13:1615-21. 2007
    ..2 of 21 in the experimental group, compared with 10.0 in the controls (p = .001). Induced ADSCs supported in a fibrin glue matrix are a promising cell source for cartilage tissue engineering...
  26. ncbi request reprint Adult stem cell therapy for the heart
    John K Fraser
    MacroPore Biosurgery Inc, San Diego, CA 92121, USA
    Int J Biochem Cell Biol 36:658-66. 2004
    ..By contrast, demonstration of the ability of adult stem cells to undergo cardiomyocyte differentiation both in vitro and in vivo suggests a potential for regenerative medicine. This potential is being examined in early clinical studies...

Research Grants3

  1. Stimulation of ASC Osteogenic Potential In vivo
    Patricia Zuk; Fiscal Year: 2007
    ....
  2. Healing of Craniofacial Defects using Adipose-Derived Stem Cells
    Patricia A Zuk; Fiscal Year: 2010
    ..Such a strategy, if successful, may also have applicability to other common craniofacial abnormalities in children thus decreasing the substantial financial burden imposed each year on the US medical system. ..