Inhibition of the mitochondrial permeability transition by cyclosporin A prevents pyrazole plus lipopolysaccharide-induced liver injury in miceJian Zhuge
Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
Free Radic Biol Med 46:406-13. 2009
..These results indicate that mitochondria are the critical targets of pyrazole plus LPS in mediating liver injury...
A decrease in S-adenosyl-L-methionine potentiates arachidonic acid cytotoxicity in primary rat hepatocytes enriched in CYP2E1Jian Zhuge
Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
Mol Cell Biochem 314:105-12. 2008
..Such interactions, e.g. induction of CYP2E1, decline in SAM and polyunsaturated fatty acid-induced lipid peroxidation, may contribute to alcohol-induced liver injury...
- Depletion of S-adenosyl-l-methionine with cycloleucine potentiates cytochrome P450 2E1 toxicity in primary rat hepatocytes
Jian Zhuge
Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
Arch Biochem Biophys 466:177-85. 2007
..This is due to elevated ROS production by CYP2E1 coupled to lower levels of hepatoprotective SAM and GSH. We speculate that such interactions e.g. induction of CYP2E1, decline in SAM and GSH may contribute to alcohol liver toxicity...
- Overexpression of CYP2E1 induces HepG2 cells death by the AMP kinase activator 5'-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR)
Jian Zhuge
Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
Cell Biol Toxicol 25:253-63. 2009
..The cytotoxicity mechanism of AICAR in E47 cells is suggested to include AMPK activation, p53 phosphorylation, p21 expression, overexpression of CYP2E1, and intracellular ROS accumulation...
Increased toxicity by transforming growth factor-beta 1 in liver cells overexpressing CYP2E1Jian Zhuge
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, One Gustave L Place, New York, NY 10029, USA
Free Radic Biol Med 41:1100-12. 2006
..Potentiation of TGF-beta1-induced cell death by CYP2E1 may contribute to mechanisms of alcohol-induced liver disease...
- Serum deprivation-induced HepG2 cell death is potentiated by CYP2E1
Jian Zhuge
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, One Gustave L Place, New York, NY 10029, USA
Free Radic Biol Med 40:63-74. 2006
..Potentiation of serum deprivation-induced cell death by CYP2E1 may contribute to the sensitivity of the liver to alcohol-induced ischemia and growth factor deprivation...
- Cytochrome P450 2E1 contributes to ethanol-induced fatty liver in mice
Yongke Lu
Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, NY 10029, USA
Hepatology 47:1483-94. 2008
....
- Ethanol induction of CYP2A5: permissive role for CYP2E1
Yongke Lu
Department of Medicine, Division of Liver Diseases, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
Drug Metab Dispos 39:330-6. 2011
..The possibility that induction of CYP2E1 is permissive for the induction of CYP2A5 may reflect a new contribution by CYP2E1 to the actions of ethanol...
- By-product formation by a novel glycerol-producing yeast, Candida glycerinogenes, with different O2 supplies
Hairu Jin
School of Biotechnology, Southern Yangtze University, Huihe Road No 170, Wuxi, Jiangsu Province 214036, PR China
Biotechnol Lett 25:311-4. 2003
..6 g l(-1)) was produced. In addition, ethanol decreased to 0.4 g l(-1) and the glycerol yield improved from 34 to 50% (w/w) by adding 50 g sulphite l(-1), nevertheless, acetic acid increased to 7.8 g l(-1)...
- Isolation and sequence analysis of the gene URA3 encoding the orotidine-5'-phosphate decarboxylase from Candida glycerinogenes WL2002-5, an industrial glycerol producer
Yanli Li
The Key Lab of Industrial Biotechnology of Ministry of Education, Research Centre of Industrial Microorganisms, Southern Yangtz University, Wuxi 214036, People's Republic of China
Yeast 22:423-30. 2005
..65% amino acid sequence similarity to the S. cerevisiae URA3 protein. Furthermore, the cloned ORF fully complemented the ura3 mutation of S. cerevisiae, confirming that it encodes for the C. glycerinogenes Ura3 (CgUra3) protein...
- An efficient genetic transformation method for glycerol producer Candida glycerinogenes
Xianzhong Chen
The Key Lab of Industrial Biotechnology, The Ministry of Education and Research Center of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi 214122, China
Microbiol Res 163:531-7. 2008
..With this method, a maximal transformation frequency of 394+/-50transformants/mug plasmid DNA was obtained. The transformation method will foster genetic manipulation of this industrial strain...
- Heterologous expression of human cytochrome P450 2E1 in HepG2 cell line
Jian Zhuge
Department of Pathophysiology, School of Medicine, Zhejiang University, Hangzhou 310031, Zhejiang Province, China
World J Gastroenterol 9:2732-6. 2003
..To assess the metabolic and toxicological characteristics of CYP2E1, we cloned CYP2E1 cDNA and established a HepG2 cell line stably expressing recombinant CYP 2E1...
- Cloning and characterization of a NAD+-dependent glycerol-3-phosphate dehydrogenase gene from Candida glycerinogenes, an industrial glycerol producer
Xianzhong Chen
Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi, China
FEMS Yeast Res 8:725-34. 2008
..cerevisiae mutants transformed with the empty plasmid showed only slight increases. The full-length CgGPD gene sequence including upstream and downstream regions has been deposited in GenBank under accession no. EU186536...
- Cloning and over-expression of an alkaline protease from Bacillus licheniformis
Xue Ming Tang
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microbiology, Southern Yangtze University, Wuxi 214036, P R China
Biotechnol Lett 26:975-9. 2004
..SDS-PAGE analysis indicated a Mr of 30.5 kDa. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 6816...
- Three new alternative splicing variants of human cytochrome P450 2D6 mRNA in human extratumoral liver tissue
Jian Zhuge
Department of Pathophysiology, Environmental Genomics Center, School of Medicine, Zhejiang University, Hangzhou 310031, Zhejiang Province, China
World J Gastroenterol 10:3356-60. 2004
..To identify the new alternative splicing variants of human CYP2D6 in human extratumoral liver tissue with RT-PCR and sequencing...
- Construction of a flocculating yeast for fuel ethanol production
Fu Zhuan Wang
Research Center of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China
Biotechnol Lett 30:97-102. 2008
..5 to 6.0. Furthermore, the ethanol concentration and other properties of the transformant strain ZWA46-F2 were similar to those of the wild-type strain ZWA46...
- Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis
Xue Ming Tang
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Research Center of Industrial Microbiology, Southern Yangtze University, Wuxi 214036, PR China
Biotechnol Lett 26:1421-4. 2004
..It was optimally active at pH 11 and at 60 degrees C (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition...
- [Research progress on the glycerol kinase]
Xuena Guo
Key Laboratory of Industrial Biotechnology, Ministry of Education Southern Yangtes University, Wuxi 214036, China
Wei Sheng Wu Xue Bao 42:510-3. 2002
