Posttranslation modification of G protein-coupled receptor in relationship to biased agonismHui Zheng
Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
Methods Enzymol 522:391-408. 2013
..A functional model was also provided on these posttranslational modifications at the last section of this chapter...
Palmitoylation and membrane cholesterol stabilize μ-opioid receptor homodimerization and G protein couplingHui Zheng
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
BMC Cell Biol 13:6. 2012
..We sought to investigate whether a similar phenomenon could be observed with μ-opioid receptor (OPRM1), and if so, to assess the role of cholesterol in this class of G protein-coupled receptor (GPCR) signaling...
- Modulating micro-opioid receptor phosphorylation switches agonist-dependent signaling as reflected in PKCepsilon activation and dendritic spine stability
Hui Zheng
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455 0217, USA
J Biol Chem 286:12724-33. 2011
..Phosphorylation blockage made etorphine, fentanyl, and DAMGO function as morphine in the primary cultures. Therefore, agonist-dependent phosphorylation of GPCR regulates the activation of the PKC pathway and the subsequent responses...
- Modulations of NeuroD activity contribute to the differential effects of morphine and fentanyl on dendritic spine stability
Hui Zheng
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455 0217, USA
J Neurosci 30:8102-10. 2010
..Furthermore, elevating NeuroD activity attenuated the morphine-induced decrease in dendritic spine stability. Therefore, by regulating NeuroD activity, mu-opioid receptor agonists modulate the stability of dendritic spines...
- Yin Yang 1 phosphorylation contributes to the differential effects of mu-opioid receptor agonists on microRNA-190 expression
Hui Zheng
Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455 0217, USA
J Biol Chem 285:21994-2002. 2010
..This study therefore delineates a signaling pathway that mediates the effects of fentanyl on miR-190 expression...
- mu-Opioid receptor agonists differentially regulate the expression of miR-190 and NeuroD
Hui Zheng
Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455 0217, USA
Mol Pharmacol 77:102-9. 2010
....
- Cholesterol level influences opioid signaling in cell models and analgesia in mice and humans
Hui Zheng
Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
J Lipid Res 53:1153-62. 2012
..Our studies also suggested that a low cholesterol level could lead to clinical issues, such as the observed impairment in opioid functions...
- MicroRNA 339 down-regulates μ-opioid receptor at the post-transcriptional level in response to opioid treatment
Qifang Wu
Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
FASEB J 27:522-35. 2013
..Taken together, these results suggest that miR-339-3p may serve as a negative feedback modulator of MOR signals by regulating intracellular MOR biosynthesis...
- Agonist-dependent mu-opioid receptor signaling can lead to heterologous desensitization
Ji Chu
Department of Pharmacology, University of Minnesota, 6 120 Jackson Hall, 321 Church St S E, Minneapolis, Minnesota 55455 0217, USA
Cell Signal 22:684-96. 2010
..Thus, depending on the agonist, activation of MOR could lead to heterologous desensitization and probable crosstalk between MOR and other Galphai-coupled receptors, such as the CB1...
- Agonist-selective signaling of G protein-coupled receptor: mechanisms and implications
Hui Zheng
Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455 0217, USA
IUBMB Life 62:112-9. 2010
..In addition, current review also provides an overview on how agonist-selective signaling is initiated. Especially, the relationship between GPCR-G protein interaction and GPCR-beta-arrestin interaction is discussed in depth...
- Agonist-selective signaling is determined by the receptor location within the membrane domains
Hui Zheng
Department of Pharmacology, University of Minnesota Medical School, 6 120 Jackson Hall, 321 Church Street Southeast, Minneapolis, MN 55455 0217, USA
Proc Natl Acad Sci U S A 105:9421-6. 2008
..Thus, agonist-selective signaling is regulated by the location of MOR, which is determined by interactions of MOR with G proteins and beta-arrestin...
- Morphine-induced mu-opioid receptor rapid desensitization is independent of receptor phosphorylation and beta-arrestins
Ji Chu
Department of Pharmacology, Medical School, University of Minnesota, Minneapolis, MN 55455 0217, USA
Cell Signal 20:1616-24. 2008
..Hence, morphine could induce OPRM1 desensitization via pathway independent of betaArr, thus suggesting the in vivo tolerance development to morphine can occur in the absence of betaArr...
- Complete lobar torsion simulating hemorrhagic shock after left upper lobectomy
Chang Chen
Department of Thoracic Surgery, Tongji University Affiliated Shanghai Pulmonary Hospital, Shanghai, China
Asian Cardiovasc Thorac Ann 17:191-3. 2009
..The profound symptom was impending hemorrhagic shock, which masqueraded as postoperative bleeding. The differentials between these 2 entities and treatment options for lung torsion are discussed...
Extralobar pulmonary sequestration in the hilumChang Chen
Department of Thoracic Surgery, Tongji University Affiliated Shanghai Pulmonary Hospital, Shanghai, China
Asian Cardiovasc Thorac Ann 16:246-8. 2008
..An unusual case of extralobar pulmonary sequestration, presenting initially as a hilar bronchogenic cyst in a 43-year-old female, is described...
- Prognosis and staging of superficial endobronchial lung cancer: the impact of invasion depth, tumor diameter, and coexistent pneumonitis or atelectasis
Chang Chen
Department of Thoracic Surgery, Shanghai Pulmonary Hospital, TongjiUniversity School of Medicine, Shanghai 200433, China
Chin Med J (Engl) 123:1505-9. 2010
..Little is known as to whether T status is impacted by the existence of associated atelectasis or pneumonia (which might be controversial, indicating either T1 or T2), and circumstantial invasion depth...
- Antiphospholipid antibody profiles in lupus nephritis with glomerular microthrombosis: a prospective study of 124 cases
Hui Zheng
Department of Rheumatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, PR China
Arthritis Res Ther 11:R93. 2009
..The purpose of this study was to investigate the prevalence of GMT in LN patients and examine the relation between the aPL profiles (including some protease-reactive aPL) and GMT...
- [Mutations and polymorphisms of the P gene associated with oculocutaneous albinism type II]
Hong lei Duan
Department of Medical Genetics, Zhongshan Medical College, SunYat sen University, Guangzhou 510089, China
Yi Chuan 27:984-8. 2005
..Some non-pathologic missense mutations may be associated with phenotypic variation in normally pigmented individuals and need to further study...
- Beta-arrestin-dependent mu-opioid receptor-activated extracellular signal-regulated kinases (ERKs) Translocate to Nucleus in Contrast to G protein-dependent ERK activation
Hui Zheng
Department of Pharmacology, Medical School, University of Minnesota 6 120 Jackson Hall, 321 Church Street S E, Minneapolis, MN 55455 0217, USA
Mol Pharmacol 73:178-90. 2008
..Thus, the cellular location of phosphorylated ERKs and subsequent activities on gene transcriptions are dictated by the agonist used to activate the receptor and the subsequent signaling pathway involved...
- NeuroD modulates opioid agonist-selective regulation of adult neurogenesis and contextual memory extinction
Hui Zheng
Key Laboratory of Regenerative Biology of CAS, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou, China
Neuropsychopharmacology 38:770-7. 2013
..Such drug-related memory regulation could have implications in eventual context-associated relapse...
- Non-Coding RNAs Regulating Morphine Function: With Emphasis on the In vivo and In vitro Functions of miR-190
Hui Zheng
Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou, China
Front Genet 3:113. 2012
..After discussing the possible targets of ncRNAs involved in the development of opioid addiction, we summarize the mechanisms underlying the interaction between ncRNAs and opioid addiction and present suggestions for further study...
