Alfeu Zanotto-Filho

Summary

Publications

  1. doi request reprint Autophagy inhibition improves the efficacy of curcumin/temozolomide combination therapy in glioblastomas
    Alfeu Zanotto-Filho
    Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, RS, Brazil Electronic address
    Cancer Lett 358:220-31. 2015
  2. doi request reprint Proteasome inhibitor MG132 induces selective apoptosis in glioblastoma cells through inhibition of PI3K/Akt and NFkappaB pathways, mitochondrial dysfunction, and activation of p38-JNK1/2 signaling
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, Rio Grande do Sul, Brasil
    Invest New Drugs 30:2252-62. 2012
  3. doi request reprint The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, Rio Grande do Sul, Brasil
    J Nutr Biochem 23:591-601. 2012
  4. doi request reprint NFκB inhibitors induce cell death in glioblastomas
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
    Biochem Pharmacol 81:412-24. 2011
  5. ncbi request reprint Retinol induces the ERK1/2-dependent phosphorylation of CREB through a pathway involving the generation of reactive oxygen species in cultured Sertoli cells
    Daniel P Gelain
    Centro de Estudos em Estresse Oxidativo, Brazil
    Cell Signal 18:1685-94. 2006
  6. doi request reprint The pharmacological NFkappaB inhibitors BAY117082 and MG132 induce cell arrest and apoptosis in leukemia cells through ROS-mitochondria pathway activation
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, UFRGS, Rio Grande do Sul, Brazil
    Cancer Lett 288:192-203. 2010
  7. doi request reprint In vitro optimization of retinoic acid-induced neuritogenesis and TH endogenous expression in human SH-SY5Y neuroblastoma cells by the antioxidant Trolox
    Mario Luiz Conte da Frota Junior
    Center of Oxidative Stress Research, Department of Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, Porto Alegre, RS 90035 003, Brazil
    Mol Cell Biochem 358:325-34. 2011
  8. doi request reprint Retinoic acid induces apoptosis by a non-classical mechanism of ERK1/2 activation
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
    Toxicol In Vitro 22:1205-12. 2008
  9. doi request reprint Retinol increases catalase activity and protein content by a reactive species-dependent mechanism in Sertoli cells
    Daniel Pens Gelain
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Chem Biol Interact 174:38-43. 2008
  10. ncbi request reprint Retinol and retinoic acid increase MMP-2 activity by different pathways in cultured Sertoli cells
    Rodrigo J S Dalmolin
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil
    Free Radic Res 41:1338-47. 2007

Collaborators

  • Daniel Pens Gelain
  • Elizandra Braganhol
  • Fabio Klamt
  • Ana Maria O Battastini
  • Felipe Dal-Pizzol
  • Rodrigo J S Dalmolin
  • Guilherme Antonio Behr
  • Martin Cammarota
  • Rafael Calixto Bortolin
  • Fernanda Freitas Caregnato
  • Andressa Bernardi
  • Clarissa Severino Gama
  • Fares Zeidán-Chuliá
  • Rodrigo Lorenzi
  • Diego Bonatto
  • Ivan Izquierdo
  • Marcio Soares
  • Jose Claudio Fonseca Moreira
  • Juciano Gasparotto
  • Fabrício Figueiró
  • Daniéli Gerhardt
  • Marcelo Sartori Grunwald
  • Christianne G Salbego
  • André Simões Pires
  • Karine Zortea
  • Mario Luiz Conte da Frota Junior
  • Valquiria Linck Bassani
  • Iuri Marques de Oliveira
  • Mauro Antonio Alves Castro
  • Matheus Augusto de Bittencourt Pasquali
  • Matheus A B Pasquali
  • Mara Regina Netto Benetti
  • Evandro Gomes da Silva
  • José Cláudio Moreira Fonseca
  • Diogo Ribeiro Demartini
  • Fabrícia Dietrich
  • Francini Kiyono Jorge Yatsu
  • Karla Suzana Moresco
  • Nauana Somensi
  • Cinthia Maria Schöler
  • Maria do Carmo Bassols Raseira
  • Marcia Vizzotto
  • Alice Kunzler
  • Gabriela Bertola
  • Carlos Eduardo Schnorr
  • Carlos Henrique Barrios
  • Carolina Saibro Girardi
  • Fernanda Bueno Morrone
  • Thallita Kelly Rabelo
  • Paulo Ivo Homem de Bittencourt
  • Jose Claudio F Moreira
  • Rudimar L Frozza
  • Elisa H F Jandrey
  • Silvia S Guterres
  • Thatiana Terroso
  • Adriana R Pohlmann
  • Maria I Edelweiss
  • Lísia Rejane Guimarães
  • Rafael Schroeder
  • Paulo Silva Belmonte-de-Abreu
  • Brisa Simões Fernandes
  • Camila Lersch
  • Maria Inês Rodrigues Lobato
  • Lenise Petter Francesconi
  • José Cláudio Moreira
  • Fernanda M Lopes
  • Ivi Juliana Bristot
  • João Antonio Pêgas Henriques
  • Emily Shacter
  • Marilda da Cruz Fernandes
  • Fernanda Martins Lopes
  • Stephanie Zdanov
  • Carolina Beatriz Muller
  • Ramatis B de Oliveira
  • Luiz F de Souza
  • Jose C F Moreira
  • Marcos Roberto de Oliveira
  • Andréa Gisiane Kurek
  • Martina Rudnicki
  • Francilene Amaral da Silva
  • Luis Gustavo Ravazolo
  • Gilberto Schwartsmann
  • Ana Lúcia Aboy
  • Adriana Coitinho
  • Celso Luis Bordignon
  • Amélia Teresinha Henriques
  • Flávio Henrique Reginatto

Detail Information

Publications26

  1. doi request reprint Autophagy inhibition improves the efficacy of curcumin/temozolomide combination therapy in glioblastomas
    Alfeu Zanotto-Filho
    Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, RS, Brazil Electronic address
    Cancer Lett 358:220-31. 2015
    ..Overall, the data presented demonstrate that autophagy impairs the efficacy of temozolomide/curcumin, and inhibiting this phenomenon could provide novel opportunities to improve brain tumor treatment. ..
  2. doi request reprint Proteasome inhibitor MG132 induces selective apoptosis in glioblastoma cells through inhibition of PI3K/Akt and NFkappaB pathways, mitochondrial dysfunction, and activation of p38-JNK1/2 signaling
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, Rio Grande do Sul, Brasil
    Invest New Drugs 30:2252-62. 2012
    ..In summary, MG132 exerted profound and selective toxicity in GBMs, being a potential agent for further testing in animal models of the disease...
  3. doi request reprint The curry spice curcumin selectively inhibits cancer cells growth in vitro and in preclinical model of glioblastoma
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, Rio Grande do Sul, Brasil
    J Nutr Biochem 23:591-601. 2012
    ..In summary, data presented here suggest curcumin as a potential agent for therapy of GBMs...
  4. doi request reprint NFκB inhibitors induce cell death in glioblastomas
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
    Biochem Pharmacol 81:412-24. 2011
    ..These findings support NFκB as a potential target to cell death induction in GBMs, and that the NFκB inhibitors may be considered for in vivo testing on animal models and possibly on GBM therapy...
  5. ncbi request reprint Retinol induces the ERK1/2-dependent phosphorylation of CREB through a pathway involving the generation of reactive oxygen species in cultured Sertoli cells
    Daniel P Gelain
    Centro de Estudos em Estresse Oxidativo, Brazil
    Cell Signal 18:1685-94. 2006
    ....
  6. doi request reprint The pharmacological NFkappaB inhibitors BAY117082 and MG132 induce cell arrest and apoptosis in leukemia cells through ROS-mitochondria pathway activation
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, UFRGS, Rio Grande do Sul, Brazil
    Cancer Lett 288:192-203. 2010
    ..Data suggest that the NFkappaB inhibitors MG132 and BAY117082 are potential anti-leukemia agents...
  7. doi request reprint In vitro optimization of retinoic acid-induced neuritogenesis and TH endogenous expression in human SH-SY5Y neuroblastoma cells by the antioxidant Trolox
    Mario Luiz Conte da Frota Junior
    Center of Oxidative Stress Research, Department of Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, Porto Alegre, RS 90035 003, Brazil
    Mol Cell Biochem 358:325-34. 2011
    ..Therefore, our data demonstrate that concomitant decreases in basal reactive oxygen species (ROS) production could exert a positive effect on the neuritogenic process of RA-treated SH-SY5Y cells...
  8. doi request reprint Retinoic acid induces apoptosis by a non-classical mechanism of ERK1/2 activation
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
    Toxicol In Vitro 22:1205-12. 2008
    ..The non-canonical RA signaling presented in this work evokes new perspectives of RA action, which may play an important role in mediating early biological effects of RA modulating cell death in normal and tumor cells...
  9. doi request reprint Retinol increases catalase activity and protein content by a reactive species-dependent mechanism in Sertoli cells
    Daniel Pens Gelain
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Chem Biol Interact 174:38-43. 2008
    ....
  10. ncbi request reprint Retinol and retinoic acid increase MMP-2 activity by different pathways in cultured Sertoli cells
    Rodrigo J S Dalmolin
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil
    Free Radic Res 41:1338-47. 2007
    ..Our findings suggested that retinol-induced MMP-2 activity, but not retinoic acid-induced MMP-2 activity, was related to ERK1/2 phosphorylation and ROS production...
  11. doi request reprint Retinol and retinoic acid modulate catalase activity in Sertoli cells by distinct and gene expression-independent mechanisms
    Matheus A B Pasquali
    CEEO, Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Laboratório 32, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, CEP 90035003, Porto Alegre, RS, Brazil
    Toxicol In Vitro 22:1177-83. 2008
    ..In vitro incubation of purified CAT with retinol or RA did not alter enzyme activity...
  12. doi request reprint The role of two putative nitroreductases, Frm2p and Hbn1p, in the oxidative stress response in Saccharomyces cerevisiae
    Iuri Marques de Oliveira
    Departamento de Biofisica Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av Bento Goncalves 9500, 91507 970 Porto Alegre, RS, Brazil
    Yeast 27:89-102. 2010
    ..In summary, the Frm1p and Hbn1p nitroreductases influence the response to oxidative stress in S. cerevisae yeast by modulating the GSH contents and antioxidant enzymatic activities, such as SOD, CAT and GPx...
  13. doi request reprint Differential effects of retinol and retinoic acid on cell proliferation: a role for reactive species and redox-dependent mechanisms in retinol supplementation
    Alfeu Zanotto-Filho
    Departamento de Bioquimica, Centro de Estudos em Estresse Oxidativo, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil
    Free Radic Res 42:778-88. 2008
    ..RA decreased DNA synthesis and increased p21 leading to cell arrest. Overall, data show that ROH, but not RA, is able to induce proliferation through non-classical and redox-dependent mechanisms...
  14. doi request reprint Xanthine oxidase-dependent ROS production mediates vitamin A pro-oxidant effects in cultured Sertoli cells
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
    Free Radic Res 42:593-601. 2008
    ..The data show that xanthine oxidase may play a role on vitamin A pro-oxidant effects...
  15. ncbi request reprint Resveratrol-loaded lipid-core nanocapsules treatment reduces in vitro and in vivo glioma growth
    Fabrício Figueiró
    Departamento de Bioquimica, Institute de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90035 003, Brasil
    J Biomed Nanotechnol 9:516-26. 2013
    ....
  16. doi request reprint Curcumin-loaded lipid-core nanocapsules as a strategy to improve pharmacological efficacy of curcumin in glioma treatment
    Alfeu Zanotto-Filho
    Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, Rio Grande do Sul, Brazil
    Eur J Pharm Biopharm 83:156-67. 2013
    ..Considered overall, the data suggest that the nanoencapsulation of curcumin in LNC is an important strategy to improve its pharmacological efficacy in the treatment of gliomas...
  17. ncbi request reprint Evaluation of antioxidant effect of extracts of Symphyopappus casarettoi
    Mara Regina Netto Benetti
    Curso de Pós Graduação em Ciências Biológicas Bioquímica, Instituto de Ciências Básicas de Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, Porto Alegre, RS, CEP 90035 003, Brazil
    Fitoterapia 78:232-4. 2007
    ..These findings suggest that the ethanolic extract of S. casarettoi inflorescence and its methanolic fraction have in vitro and ex vivo antioxidant properties...
  18. doi request reprint Retinol up-regulates the receptor for advanced glycation endproducts (RAGE) by increasing intracellular reactive species
    Daniel Pens Gelain
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Laboratório 32, Rua Ramiro Barcelos 2600 anexo, CEP 90035 003, Porto Alegre, RS, Brazil
    Toxicol In Vitro 22:1123-7. 2008
    ....
  19. doi request reprint Reduced serum non-enzymatic antioxidant defense and increased lipid peroxidation in schizophrenic patients on a hypocaloric diet
    Karine Zortea
    Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    Neurosci Lett 512:43-7. 2012
    ..Therefore, we aimed to study the effect of a hypocaloric dietary intervention on OS in subjects with SZ...
  20. doi request reprint The oxidation of HSP70 is associated with functional impairment and lack of stimulatory capacity
    Marcelo Sartori Grunwald
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande do Sul, CEP 90035 003, Brazil
    Cell Stress Chaperones 19:913-25. 2014
    ..These results indicate that oxidation of extracellular HSP70 modified its signaling properties, causing alterations on its modulatory effects on macrophage function and viability. ..
  21. pmc CFL1 expression levels as a prognostic and drug resistance marker in nonsmall cell lung cancer
    Mauro Antonio Alves Castro
    Department of Biochemistry, ICBS Federal University of Rio Grande do Sul, Porto Alegre, Brazil
    Cancer 116:3645-55. 2010
    ....
  22. ncbi request reprint Antioxidant activities and free radical scavenging potential of Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) extracts linked to their polyphenol content
    Evandro Gomes da Silva
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600, 90035 003 Porto Alegre, RS, Brazil
    Biol Pharm Bull 30:1488-96. 2007
    ..B. microstachya extracts may be considered an interesting source of natural antioxidants as well as other phenolic-rich plants...
  23. doi request reprint Boldine induces cell cycle arrest and apoptosis in T24 human bladder cancer cell line via regulation of ERK, AKT, and GSK-3β
    Daniéli Gerhardt
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul UFRGS, Porto Alegre, RS, Brasil Electronic address
    Urol Oncol 32:36.e1-9. 2014
    ..The objective of the present study was to evaluate the effect and underlying mechanisms of boldine, an aporphine alkaloid of Peumus boldus, on bladder cancer proliferation and cell death...
  24. doi request reprint The NF kappa B-mediated control of RS and JNK signaling in vitamin A-treated cells: duration of JNK-AP-1 pathway activation may determine cell death or proliferation
    Alfeu Zanotto-Filho
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    Biochem Pharmacol 77:1291-301. 2009
    ..Taken together, data presented here show that NFkappaB mediates cellular resistance to the pro-oxidant effects of vitamin A by inhibiting RS accumulation and the persistent and redox-dependent activation of JNK-AP-1 cascade...
  25. doi request reprint Preventive supplementation with fresh and preserved peach attenuates CCl4-induced oxidative stress, inflammation and tissue damage
    Juciano Gasparotto
    Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul UFRGS Brazil Electronic address
    J Nutr Biochem 25:1282-95. 2014
    ....
  26. doi request reprint Nucleotide receptors control IL-8/CXCL8 and MCP-1/CCL2 secretions as well as proliferation in human glioma cells
    Elizandra Braganhol
    Département de microbiologie infectiologie et d immunologie, Faculte de Medecine, Universite Laval, Quebec, QC G1V 0A6, Canada Centre de recherche du CHU de Québec, CHUL, Quebec, QC G1V 4G2, Canada Departamento de Bioquímica, Instituto de Ciencias Basicas da Saude, UFRGS, Porto Alegre, RS, Brazil
    Biochim Biophys Acta 1852:120-30. 2015
    ..Altogether, these data suggest that extracellular nucleotides control glioma growth via P2 receptor-dependent IL-8 and MCP-1 secretions. ..