Musheshe N, Lobo M, Schmidt M, Zaccolo M. Targeting FRET-Based Reporters for cAMP and PKA Activity Using AKAP79. Sensors (Basel). 2018;18: pubmed publisher
..By using as the targeting domain wild type AKAP79 or a mutant that cannot interact with PKA, we establish that AKAP79 does not affect the amplitude and kinetics of cAMP changes or the level of PKA activity detected by the sensor. ..
Brescia M, Zaccolo M. Modulation of Compartmentalised Cyclic Nucleotide Signalling via Local Inhibition of Phosphodiesterase Activity. Int J Mol Sci. 2016;17: pubmed
Fields L, Koschinski A, Zaccolo M. Sustained exposure to catecholamines affects cAMP/PKA compartmentalised signalling in adult rat ventricular myocytes. Cell Signal. 2016;28:725-32 pubmed publisher
..As a result, the preferential coupling of PKA subsets with different PDEs is altered resulting in a significant difference in the level of cAMP the kinase is exposed to, with potential impact on phosphorylation of downstream targets. ..
Musheshe N, Schmidt M, Zaccolo M. cAMP: From Long-Range Second Messenger to Nanodomain Signalling. Trends Pharmacol Sci. 2018;39:209-222 pubmed publisher
..These findings subvert the classical notion of cAMP signalling and provide a new framework for the development of targeted therapeutic approaches. ..
Schleicher K, Zaccolo M. Using cAMP Sensors to Study Cardiac Nanodomains. J Cardiovasc Dev Dis. 2018;5: pubmed publisher
Zoccarato A, Surdo N, Aronsen J, Fields L, Mancuso L, Dodoni G, et al
. Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2. Circ Res. 2015;117:707-19 pubmed publisher
..Different cAMP pools have opposing effects on cardiac myocyte cell size. PDE2 emerges as a novel key regulator of cardiac hypertrophy in vitro and in vivo, and its inhibition may have therapeutic applications. ..
Koschinski A, Zaccolo M. Activation of PKA in cell requires higher concentration of cAMP than in vitro: implications for compartmentalization of cAMP signalling. Sci Rep. 2017;7:14090 pubmed publisher
..Our findings have important implications for the understanding of compartmentalized cAMP signalling. ..