Elton T Young

Summary

Publications

  1. pmc The AMP-activated protein kinase Snf1 regulates transcription factor binding, RNA polymerase II activity, and mRNA stability of glucose-repressed genes in Saccharomyces cerevisiae
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 287:29021-34. 2012
  2. pmc Snf1 dependence of peroxisomal gene expression is mediated by Adr1
    Sooraj Ratnakumar
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 285:10703-14. 2010
  3. ncbi request reprint Snf1-independent, glucose-resistant transcription of Adr1-dependent genes in a mediator mutant of Saccharomyces cerevisiae
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Mol Microbiol 74:364-83. 2009
  4. pmc Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression
    Elton T Young
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
    Mol Cell Biol 28:2509-16. 2008
  5. ncbi request reprint A poised initiation complex is activated by SNF1
    Christine Tachibana
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 282:37308-15. 2007
  6. pmc Snf1 controls the activity of adr1 through dephosphorylation of Ser230
    Sooraj Ratnakumar
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    Genetics 182:735-45. 2009
  7. pmc 14-3-3 (Bmh) proteins regulate combinatorial transcription following RNA polymerase II recruitment by binding at Adr1-dependent promoters in Saccharomyces cerevisiae
    Katherine A Braun
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Mol Cell Biol 33:712-24. 2013
  8. pmc The transcriptional coactivators SAGA, SWI/SNF, and mediator make distinct contributions to activation of glucose-repressed genes
    Rhiannon K Biddick
    Department of Biochemistry, University of Washington, Seattle, Washington 98105, USA
    J Biol Chem 283:33101-9. 2008
  9. pmc Adr1 and Cat8 mediate coactivator recruitment and chromatin remodeling at glucose-regulated genes
    Rhiannon K Biddick
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 3:e1436. 2008
  10. pmc Snf1-dependent and Snf1-independent pathways of constitutive ADH2 expression in Saccharomyces cerevisiae
    Valentina Voronkova
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Genetics 172:2123-38. 2006

Collaborators

  • Kenneth M Dombek
  • G Lynn Law
  • Benjamin P Tu
  • Ruedi Aebersold
  • Chao Zhang
  • Trey Ideker
  • Pabitra K Parua
  • Rachel E Mohler
  • Christine Tachibana
  • Katherine A Braun
  • Robert E Synovec
  • Nataly Kacherovsky
  • Rhiannon Biddick
  • Sooraj Ratnakumar
  • Elizabeth M Humston
  • Rhiannon K Biddick
  • Jamin C Hoggard
  • Juan José Infante
  • Juan J Infante
  • Steven Hahn
  • Valentina Voronkova
  • Fang Fang
  • Salvator Palmisano
  • Stefania Vaga
  • Gregory E Miner
  • Paul M Ryan
  • Kayla Trang
  • I Ting Wang
  • Hsin Wen Ella Chang
  • Erin Arms
  • David Fox
  • Emily Amos
  • Kevin Khaw Beng Chin
  • Karisa M Pierce
  • Diana Yu
  • Jane Y Yoo
  • Jean Basco Tagne
  • Tong I Lee

Detail Information

Publications29

  1. pmc The AMP-activated protein kinase Snf1 regulates transcription factor binding, RNA polymerase II activity, and mRNA stability of glucose-repressed genes in Saccharomyces cerevisiae
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 287:29021-34. 2012
    ..In conclusion, Snf1 appears to affect multiple steps in gene regulation, including transcription factor binding, RNA polymerase II activity, and cytoplasmic mRNA stability...
  2. pmc Snf1 dependence of peroxisomal gene expression is mediated by Adr1
    Sooraj Ratnakumar
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 285:10703-14. 2010
    ....
  3. ncbi request reprint Snf1-independent, glucose-resistant transcription of Adr1-dependent genes in a mediator mutant of Saccharomyces cerevisiae
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Mol Microbiol 74:364-83. 2009
    ..Expression of most genes was not significantly affected by the loss of Med14 function...
  4. pmc Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression
    Elton T Young
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
    Mol Cell Biol 28:2509-16. 2008
    ..Artificial recruitment of Mediator appears to overcome this requirement and to allow stable binding and transcription under normally inhibitory conditions...
  5. ncbi request reprint A poised initiation complex is activated by SNF1
    Christine Tachibana
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 282:37308-15. 2007
    ....
  6. pmc Snf1 controls the activity of adr1 through dephosphorylation of Ser230
    Sooraj Ratnakumar
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    Genetics 182:735-45. 2009
    ....
  7. pmc 14-3-3 (Bmh) proteins regulate combinatorial transcription following RNA polymerase II recruitment by binding at Adr1-dependent promoters in Saccharomyces cerevisiae
    Katherine A Braun
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Mol Cell Biol 33:712-24. 2013
    ..Activating Snf1 by deleting Reg1, a Glc7 protein phosphatase regulatory subunit, is lethal in combination with defective Bmh in strain W303, suggesting that Bmh and Snf1 have opposing roles in an essential cellular process...
  8. pmc The transcriptional coactivators SAGA, SWI/SNF, and mediator make distinct contributions to activation of glucose-repressed genes
    Rhiannon K Biddick
    Department of Biochemistry, University of Washington, Seattle, Washington 98105, USA
    J Biol Chem 283:33101-9. 2008
    ..Finally, we found that activator overexpression can compensate for the loss of SWI/SNF but not for the loss of SAGA...
  9. pmc Adr1 and Cat8 mediate coactivator recruitment and chromatin remodeling at glucose-regulated genes
    Rhiannon K Biddick
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 3:e1436. 2008
    ..Adr1 and Cat8 co-regulate numerous glucose-repressed genes in S. cerevisiae, presenting a unique opportunity to explore their individual roles in coactivator recruitment, chromatin remodeling, and transcription...
  10. pmc Snf1-dependent and Snf1-independent pathways of constitutive ADH2 expression in Saccharomyces cerevisiae
    Valentina Voronkova
    Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
    Genetics 172:2123-38. 2006
    ..Thus, the mutant search revealed previously unknown Snf1-dependent and -independent pathways of ADH2 expression...
  11. pmc Promoter binding by the Adr1 transcriptional activator may be regulated by phosphorylation in the DNA-binding region
    Nataly Kacherovsky
    Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 3:e3213. 2008
    ..A fusion protein with the Adr1 DNA-binding domain and a heterologous activation domain is glucose-regulated, suggesting that the DNA binding region is the target of regulation...
  12. ncbi request reprint Multiple pathways are co-regulated by the protein kinase Snf1 and the transcription factors Adr1 and Cat8
    Elton T Young
    Department of Biochemistry, The University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 278:26146-58. 2003
    ..The largest class of SNF1-dependent genes encodes regulatory proteins that could extend SNF1 dependence to additional pathways...
  13. pmc Combined global localization analysis and transcriptome data identify genes that are directly coregulated by Adr1 and Cat8
    Christine Tachibana
    Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
    Mol Cell Biol 25:2138-46. 2005
    ..These data provide a comprehensive analysis of the direct, indirect, and combinatorial requirements for these two global transcription factors...
  14. pmc Identification and evaluation of cycling yeast metabolites in two-dimensional comprehensive gas chromatography-time-of-flight-mass spectrometry data
    Rachel E Mohler
    University of Washington, Department of Chemistry, Box 351700, Seattle, WA 98195, USA
    J Chromatogr A 1186:401-11. 2008
    ..The phase delay of the cycling metabolites ranged widely in relation to the oxygen consumption cycling pattern...
  15. ncbi request reprint The Reg1-interacting proteins, Bmh1, Bmh2, Ssb1, and Ssb2, have roles in maintaining glucose repression in Saccharomyces cerevisiae
    Kenneth M Dombek
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 279:39165-74. 2004
    ..Deletion of both SSB genes modestly decreases repression of ADH2 expression in the presence of glucose, suggesting that Ssb proteins, perhaps through their interaction with Reg1, play a minor role in glucose repression...
  16. pmc Toward a global analysis of metabolites in regulatory mutants of yeast
    Elizabeth M Humston
    Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195 7350, USA
    Anal Bioanal Chem 401:2387-402. 2011
    ....
  17. pmc Activator-independent transcription of Snf1-dependent genes in mutants lacking histone tails
    Juan J Infante
    Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
    Mol Microbiol 80:407-22. 2011
    ..Deleting REG1 in the histone H4 tail mutant appeared to be lethal, even in the absence of Snf1, suggesting that Reg1 and the H4 tail have redundant functions that are important for cell viability...
  18. doi request reprint The disorderly study of ordered recruitment
    Rhiannon Biddick
    Department of Biochemistry, University of Washington, Seattle, WA 98105, USA
    Yeast 26:205-20. 2009
    ..Taking a step back to survey these results, however, suggests that there are few generalizations that could be used to guide future studies of uncharacterized promoters...
  19. pmc Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction
    Pabitra K Parua
    Department of Biochemistry, University of Washington, 1705 NE Pacific Street, Seattle, Washington 98195 7350, USA
    Mol Microbiol 85:282-98. 2012
    ..We provide the first molecular explanation of carbon source-mediated regulation of Mxr1 activity, whose mechanism involves a post-DNA binding role of 14-3-3...
  20. pmc Time-dependent profiling of metabolites from Snf1 mutant and wild type yeast cells
    Elizabeth M Humston
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Anal Chem 80:8002-11. 2008
    ..For the yeast strains examined in the current study, data collection at either 4 or 6 h provided information comparable to averaged time course data, albeit with a few metabolites missing using a single sampling time point...
  21. pmc Cyclic changes in metabolic state during the life of a yeast cell
    Benjamin P Tu
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9038, USA
    Proc Natl Acad Sci U S A 104:16886-91. 2007
    ..Such oscillations in metabolic state might occur during the course of other biological cycles...
  22. ncbi request reprint Comprehensive analysis of yeast metabolite GC x GC-TOFMS data: combining discovery-mode and deconvolution chemometric software
    Rachel E Mohler
    University of Washington, Department of Chemistry, Box 351700, Seattle, WA 98195, USA
    Analyst 132:756-67. 2007
    ....
  23. doi request reprint Analysis of nucleosome positioning using a nucleosome-scanning assay
    Juan José Infante
    Department of Microbiology, University of Washington, Seattle, WA, USA
    Methods Mol Biol 833:63-87. 2012
    ..When coupled with chromatin immunoprecipitation (ChIP), NuSA can identify histone variants and modifications associated with specific nucleosomes...
  24. ncbi request reprint Snf1 protein kinase regulates Adr1 binding to chromatin but not transcription activation
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 277:38095-103. 2002
    ..We conclude that Snf1 regulates Adr1-dependent transcription primarily at the level of chromatin binding...
  25. pmc Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry analysis of metabolites in fermenting and respiring yeast cells
    Rachel E Mohler
    Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA
    Anal Chem 78:2700-9. 2006
    ....
  26. ncbi request reprint Yeast mediator and its role in transcriptional regulation
    Rhiannon Biddick
    Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195 7350, USA
    C R Biol 328:773-82. 2005
    ..One focal point of the review is to summarize new information regarding the negative effect of Mediator on transcription and suggest a possible mechanism that encompasses the latest results...
  27. ncbi request reprint Phosphoproteomic analysis identifies proteins involved in transcription-coupled mRNA decay as targets of Snf1 signaling
    Katherine A Braun
    Department of Biochemistry, University of Washington, 1705 Northeast Pacific Street, Seattle, WA 98195 7350, USA
    Sci Signal 7:ra64. 2014
    ..Mutation of three Snf1-dependent phosphorylation sites in Xrn1 reduced glucose-induced mRNA decay. Thus, Xrn1 is required for Snf1-dependent mRNA homeostasis in response to nutrient availability. ..
  28. pmc Transcriptional regulation in Saccharomyces cerevisiae: transcription factor regulation and function, mechanisms of initiation, and roles of activators and coactivators
    Steven Hahn
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 189:705-36. 2011
    ..We also examine advances in understanding the RNA polymerase II transcription machinery, conserved coactivator complexes, transcription activation domains, and the cooperation of these factors in gene regulatory mechanisms...
  29. pmc Binding and transcriptional regulation by 14-3-3 (Bmh) proteins requires residues outside of the canonical motif
    Pabitra K Parua
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Eukaryot Cell 13:21-30. 2014
    ..Bmh binding to the Adr1 regulatory domain, and its failure to bind when mutations are present, explains at a molecular level the transcriptional phenotype of ADR1(c) mutants. ..