Omics strategies for revealing Yersinia pestis virulenceRuifu Yang
Beijing Institute of Microbiology and Epidemiology Beijing, China
Front Cell Infect Microbiol 2:157. 2012
..These studies serve as foundations for further hypothesis-driven research and help us gain insight into Y. pestis pathogenesis...
- Humoral and cellular immune responses to Yersinia pestis infection in long-term recovered plague patients
Bei Li
Institute of Biomedicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China
Clin Vaccine Immunol 19:228-34. 2012
..Our findings have direct implications for the future design and development of effective vaccines against Y. pestis infection and the development of new target-based diagnostics...
Genomic research for important pathogenic bacteria in ChinaRuifu Yang
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China
Sci China C Life Sci 52:50-63. 2009
....
- Universal sample preparation method for characterization of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Haihong Liu
No 20, Dongdajie, Fengtai, Beijing 100071, China
Appl Environ Microbiol 73:1899-907. 2007
..This protocol is simple, rapid, and easy to perform; has excellent reproducibility; and is suitable for the construction of a mass spectrum fingerprinting database, which helps in fast bacterial identification via database searching...
- Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis
Yingli Li
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
BMC Microbiol 9:128. 2009
..The zinc uptake regulator Zur is a Zn2+-sensing metalloregulatory protein involved in the maintenance of bacterial zinc homeostasis. Up to now, regulation of zinc homeostasis by Zur is poorly understood in Y. pestis...
Genome-wide transcriptional response of Yersinia pestis to stressful conditions simulating phagolysosomal environmentsDongsheng Zhou
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, National Center for Biomedical Analysis, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Fengtai, Beijing 100071, China
Microbes Infect 8:2669-78. 2006
..This microarray analysis would provide the candidates for identifying genes or pathways required for growth and proliferation of Y. pestis in macrophages...
- Genomic comparison of Yersinia pestis and Yersinia pseudotuberculosis by combination of suppression subtractive hybridization and DNA microarray
Xiaoyi Wang
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Arch Microbiol 186:151-9. 2006
..The results confirmed our earlier hypothesis that Y. pestis Antiqua isolates from the natural plague focus B in China represented the most ancestral strains in China, hence phylogenetically the closest isolates to Y. pseudotuberculosis...
- Identification of different regions among strains of Yersinia pestis by suppression subtractive hybridization
Erhei Dai
Laboratory of Analytical Microbiology, Institute of Microbiology and Epidemiology, National Center for Biomedical Analysis, Academy of Military Medical Sciences (AMMS, Beijing 100071, China
Res Microbiol 156:785-9. 2005
..pseudotuberculosis (serotypes I and II), showing that not all Antiqua strains had DFR4. Five DFR profiles were identified based on the presence or absence of these four DFRs in 636 strains of Y. pestis from 10 plague foci in China...
- Pseudogene accumulation might promote the adaptive microevolution of Yersinia pestis
Zongzhong Tong
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20 Dongdajie, Fengtai, Beijing 100071, China
J Med Microbiol 54:259-68. 2005
..pestis in China except for isolates from foci L and M, which might be a special lineage of Y. pestis and originated differently to the others...
- Long-term observation of subunit vaccine F1-rV270 against Yersinia pestis in mice
Zuyun Wang
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
Clin Vaccine Immunol 17:199-201. 2010
..Antibodies to F1 and rV270 were still detectable over a period of 518 days. The complete protection against lethal challenge of Yersinia pestis could be achieved up to day 518 after primary immunization...
- Autoregulation of PhoP/PhoQ and positive regulation of the cyclic AMP receptor protein-cyclic AMP complex by PhoP in Yersinia pestis
Yiquan Zhang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
J Bacteriol 195:1022-30. 2013
..The CRP and PhoP regulons have evolved to merge into a single regulatory cascade in Y. pestis because of the direct regulatory association between PhoP/PhoQ and CRP-cAMP...
- Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress
Yanping Han
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS, Beijing 100071, China
Res Microbiol 156:403-15. 2005
..pestis still executed common mechanisms to accommodate both types of stress...
- Global gene expression profile of Yersinia pestis induced by streptomycin
Jingfu Qiu
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS, Beijing 100071, China
FEMS Microbiol Lett 243:489-96. 2005
....
- The low-salt stimulon in Vibrio parahaemolyticus
Lei Yang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
Int J Food Microbiol 137:49-54. 2010
..The above differentially regulated genes will provide novel candidates for the further investigation of the molecular mechanisms of osmoadaptation in V. parahaemolyticus...
- Identification and characterization of PhoP regulon members in Yersinia pestis biovar Microtus
Yingli Li
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China
BMC Genomics 9:143. 2008
..Our previous microarray analysis suggested that PhoP governed a wide set of cellular pathways in Y. pestis. A series of biochemical experiments were done herein to study members of the PhoP regulon of Y. pestis biovar Microtus...
- A novel genotyping scheme for Vibrio parahaemolyticus with combined use of large variably-presented gene clusters (LVPCs) and variable-number tandem repeats (VNTRs)
Xiao Xiao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, China
Int J Food Microbiol 149:143-51. 2011
..A genetic fingerprint-like database of a large collection of strains established with this two-stage approach would be very useful for identification, genotyping, origin tracing, and risk estimation of V. parahaemolyticus...
- Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci
Yanjun Li
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 4:e6000. 2009
....
- Transcriptional profiling of a mice plague model: insights into interaction between Yersinia pestis and its host
Haihong Liu
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China
J Basic Microbiol 49:92-9. 2009
..pestis and expression alterations of some genes encoding cytokines in the host reflect the interaction between the pathogen and the host, which will help us better understand plague pathogenesis...
- Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis
Yiquan Zhang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People s Republic of China
PLoS ONE 6:e25484. 2011
..pestis through the regulation of various virulence-associated loci. They are the global regulators controlling two distinct large complexes of cellular pathways...
- Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis
He Gao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
BMC Microbiol 11:40. 2011
..Auto-regulation of CRP has also been established in E. coli. However, the regulation of porin genes and its own gene by CRP remains unclear in Y. pestis...
- Transcriptome analysis of the Mg2+-responsive PhoP regulator in Yersinia pestis
Dongsheng Zhou
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, National Center for Biomedical Analysis, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
FEMS Microbiol Lett 250:85-95. 2005
..pestis survives the antibacterial strategies employed by host macrophages...
- Quorum sensing affects virulence-associated proteins F1, LcrV, KatY and pH6 etc. of Yersinia pestis as revealed by protein microarray-based antibody profiling
Zeliang Chen
State Key Laboratory of Pathogen and Biosecurity, Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS, Beijing 100071, China
Microbes Infect 8:2501-8. 2006
..The results demonstrated that antibody profiling by protein microarrays is a promising high-throughput method for revealing the interactions between pathogens and the host immune system...
- Gene expression profiling of Yersinia pestis with deletion of lcrG, a known negative regulator for Yop secretion of type III secretion system
Zongmin Du
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogens and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Int J Med Microbiol 299:355-66. 2009
..Furthermore, this report also revealed significant transcriptional changes in the genes encoding cell-envelope-related proteins and a virulence-related transcription factor RovA in the DeltalcrG mutant...
- Different region analysis for genotyping Yersinia pestis isolates from China
Yanjun Li
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 3:e2166. 2008
..pestis strains for validating DFR-based genotyping method and better understanding adaptive microevolution of Y. pestis...
- Defining the genome content of live plague vaccines by use of whole-genome DNA microarray
Dongsheng Zhou
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS, Beijing 100071, PR China
Vaccine 22:3367-74. 2004
..The whole-genome DNA microarray also provides an ideal tool to perform the pre-evaluation of a vaccine strain for its high throughput to determine the genomic features essential or unallowable for the live vaccines...
- Physiological and regulatory characterization of KatA and KatY in Yersinia pestis
Yanping Han
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China
DNA Cell Biol 27:453-62. 2008
..pestis compared with that in vitro growth. However, a DeltakatY mutant was fully virulent to mice, suggesting that KatY is not required for Y. pestis virulence...
- Comparative transcriptomics in Yersinia pestis: a global view of environmental modulation of gene expression
Yanping Han
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 20, Dongdajie, Fengtai, Beijing 100071, China
BMC Microbiol 7:96. 2007
..Using cDNA microarray technology, we have analyzed the global gene expression of this deadly pathogen when grown under different stress conditions in vitro...
- Yersinia genome diversity disclosed by Yersinia pestis genome-wide DNA microarray
Xiaoyi Wang
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Can J Microbiol 53:1211-21. 2007
..pseudotuberculosis, indicating that its different serotypes have undergone an extensively parallel loss or acquisition of genetic content, which is likely to be important for its adaptation to changes in environmental niches...
- High-throughput identification of new protective antigens from a Yersinia pestis live vaccine by enzyme-linked immunospot assay
Bei Li
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China
Infect Immun 77:4356-61. 2009
..pestis, but only YPO0606 could partially protect mice from infection with Y. pestis at a higher challenge dosage (200x LD(50)). These proteins would be the potential components for Y. pestis vaccine development...
- Insight into bacterial virulence mechanisms against host immune response via the Yersinia pestis-human protein-protein interaction network
Huiying Yang
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China
Infect Immun 79:4413-24. 2011
..In addition, we identified vasodilator-stimulated phosphoprotein (VASP) as a novel interaction partner of YpkA and showed that YpkA could inhibit in vitro actin assembly mediated by VASP...
- Microarray expression profiling of Yersinia pestis in response to chloramphenicol
Jingfu Qiu
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, National Center for Biomedical Analysis, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China
FEMS Microbiol Lett 263:26-31. 2006
..A number of heat-shock genes were also repressed. These global transcriptional changes provide an insight into the mechanisms of action of chloramphenicol against Y. pestis...
- Global analysis of iron assimilation and fur regulation in Yersinia pestis
Dongsheng Zhou
State Key Laboratory of Pathogen and Biosecurity, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China
FEMS Microbiol Lett 258:9-17. 2006
..Using bioinformatics tools, we were able to predict the Y. pestis Fur box sequence that was clearly the over-presented motif in the promoter regions of members of the iron-Fur modulon...
- [Purification of recombinant H-NS protein of Yersinia pestis and characterization of its DNA-binding activity]
Yiquan Zhang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
Wei Sheng Wu Xue Bao 51:615-21. 2011
..The regulator protein H-NS of Yersinia pestis was expressed using the Escherichia coli BL21lambdaDE3 protein expression system, and its DNA-binding activity was characterized...
- DNA microarray analysis of the heat- and cold-shock stimulons in Yersinia pestis
Yanping Han
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS, Beijing 100071, China
Microbes Infect 7:335-48. 2005
..These results provide a set of new candidate genes for hypothesis-based investigations of their roles in stress response, host adaptation and pathogenicity of this deadly pathogen...
- Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis
Lingjun Zhan
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
BMC Microbiol 9:178. 2009
..The cyclic AMP receptor protein (CRP), a global regulator, was recently found to regulate the laterally acquired genes (pla and pst) in Y. pestis. The regulation of T3SS components by CRP is unknown...
- Genetics of metabolic variations between Yersinia pestis biovars and the proposal of a new biovar, microtus
Dongsheng Zhou
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
J Bacteriol 186:5147-52. 2004
..pestis and plague pathogenesis. Moreover, there may be the implications for development of biovar microtus strains as a potential vaccine...
- Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis
He Gao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
BMC Microbiol 11:39. 2011
..In Yersinia enterocolitica and Y. pseudotuberculosis, OmpR is required for both virulence and survival within macrophages. However, the phenotypic and regulatory roles of OmpR in Y. pestis are not yet fully understood...
- Yersinia pestis and host macrophages: immunodeficiency of mouse macrophages induced by YscW
Yujing Bi
State Key Laboratory of Pathogen and Biosecurity, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Beijing, China
Immunology 128:e406-17. 2009
..These data provide evidences to Y. pestis pathogenesis that some proteins in T3SS injectisome, such as YscW protein, might play independent roles in disrupting host defense apart from their known functions...
- Microarray analysis of temperature-induced transcriptome of Yersinia pestis
Yanping Han
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China
Microbiol Immunol 48:791-805. 2004
..Our data provided a genome-wide profile of gene transcription induced by temperature shift and should shed light on the pathogenicity and host-microbe interaction of this deadly pathogen...
- Extended MLST-based population genetics and phylogeny of Vibrio parahaemolyticus with high levels of recombination
Yanfeng Yan
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
Int J Food Microbiol 145:106-12. 2011
..V. parahaemolyticus had a typical epidemic population structure that is driven by mutation, recombination and lateral gene transfer...
- Ambient stable quantitative PCR reagents for the detection of Yersinia pestis
Shi Qu
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
PLoS Negl Trop Dis 4:e629. 2010
..In this work, a set of real-time PCR reagents for rapid detection of Y. pestis was developed with extraordinary stability at 37 degrees C...
- Genome plasticity of Vibrio parahaemolyticus: microevolution of the 'pandemic group'
Haihong Han
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
BMC Genomics 9:570. 2008
..parahaemolyticus. In the present work, a whole-genome cDNA microarray was constructed to compare the genomic contents of a collection of 174 strains of V. parahaemolyticus...
- The complete genome sequence and proteomics of Yersinia pestis phage Yep-phi
Xiangna Zhao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
J Gen Virol 92:216-21. 2011
..EU734170), and these comprise one subgroup. The other subgroup is represented by bacteriophage ΦA1122 (GenBank accession no. AY247822). Potential recombination was detected among the Yep-phi subgroup...
- Protein microarray for profiling antibody responses to Yersinia pestis live vaccine
Bei Li
Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Infect Immun 73:3734-9. 2005
..There are 11 proteins besides F1 and V antigens to which the predominant antibody response occurred, and these proteins show promise for further evaluation as candidates for subunit vaccines and/or diagnostic antigens...
- DNA microarray-based global transcriptional profiling of Yersinia pestis in multicellularity
Jingfu Qiu
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, P R China
J Microbiol 46:557-63. 2008
..Our results revealed the global gene expression of Y. pestis were changed in the formation of multicellularity, providing insights into the molecular mechanism of multicellular behaviour, which need investigating further...
- Transcriptional regulation of opaR, qrr2-4 and aphA by the master quorum-sensing regulator OpaR in Vibrio parahaemolyticus
Yiquan Zhang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 7:e34622. 2012
..harveyi, and LuxR-dependent expression of its own gene, qrr2-4 and aphA have been established in V. harveyi. Molecular regulation of target genes by the V. parahaemolyticus MQSR OpaR is still poorly understood...
- Antibody profiling in plague patients by protein microarray
Bei Li
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, No 20, Dongdajie, Fengtai, Beijing 100071, China
Microbes Infect 10:45-51. 2008
..pestis. Moreover, the lack of antibody to LcrV in the five patients in Focus F might be a challenge to our understanding of the pathogenesis of Y. pestis...
- Transcriptional regulation of ompF2, an ompF paralogue, in Yersinia pestis
He Gao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, People s Republic of China
Can J Microbiol 57:468-75. 2011
..The ompF2 transcription was dramatically enhanced with increasing medium osmolarity in the ompR null mutant background. The CRP regulator had no regulatory effect on ompF2 under the growth conditions tested...
- Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays
Zuowei Wu
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
BMC Genomics 12:219. 2011
....
- A proteome reference map and virulence factors analysis of Yersinia pestis 91001
Lei Zhou
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China
J Proteomics 75:894-907. 2012
..pestis may prepare itself with various strategies in advance for its survival when it evades the hosts. The protein identifications can be accessed through PRIDE database (http://www.ebi.ac.uk/pride) with accession number 18578-18605..
- Antibody responses to individual proteins of SARS coronavirus and their neutralization activities
Maofeng Qiu
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, China
Microbes Infect 7:882-9. 2005
....
- Cold-induced gene expression profiles of Vibrio parahaemolyticus: a time-course analysis
Lei Yang
Institute of Laboratory Animal Sciences, Chinese Academy of Medical Peking Union Medical College, Beijing, China
FEMS Microbiol Lett 291:50-8. 2009
..parahaemolyticus. Evident regulatory motifs were conserved within the cspA promoter regions of Escherichia coli and V. parahaemolyticus. These two bacteria likely use the same mechanism to regulate the cold-inducible expression of cspA...
- Comparative proteomics analyses reveal the virB of B. melitensis affects expression of intracellular survival related proteins
Yufei Wang
Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing, China
PLoS ONE 4:e5368. 2009
..The type IV secretion system encoded by the virB operon (virB) is involved in Brucella intracellular survival. However, the underlying molecular mechanisms, especially the target proteins affected by the virB, remain largely unclear...
- Reciprocal regulation of pH 6 antigen gene loci by PhoP and RovA in Yersinia pestis biovar Microtus
Yiquan Zhang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
Future Microbiol 8:271-80. 2013
..To explore the transcriptional regulation of the psaEF and psaABC loci by the RovA and PhoP regulators in Yersinia pestis...
- A new purification strategy for fraction 1 capsular antigen and its efficacy against Yersinia pestis virulent strain challenge
Tang Wang
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China
Protein Expr Purif 61:7-12. 2008
..pestis virulent strain 141...
- Involvement of the post-transcriptional regulator Hfq in Yersinia pestis virulence
Jing Geng
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 4:e6213. 2009
..Sequence comparisons revealed that Y. pestis appears to produce a functional homologue of E. coli Hfq...
- DNA microarray analysis of genome dynamics in Yersinia pestis: insights into bacterial genome microevolution and niche adaptation
Dongsheng Zhou
Laboratory of Analytical Microbiology, National Centre for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
J Bacteriol 186:5138-46. 2004
..Our data strongly support that the modern plague originated from Yunnan Province in China, due to the arising of biovar orientalis from biovar antiqua rather than mediaevalis...
- Antigenicity analysis of different regions of the severe acute respiratory syndrome coronavirus nucleocapsid protein
Zeliang Chen
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Clin Chem 50:988-95. 2004
..Conformational epitopes are important for this protein, and antigenicity of the COOH terminus is higher than that of the NH(2) terminus. The N protein is a potential diagnostic antigen and vaccine candidate for SARS-CoV...
- Serum cytokine responses in primary pneumonic plague patients
Xiaoyi Wang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No 20 Dongdajie, Fentai, Beijing 100071, China
Clin Vaccine Immunol 18:184-6. 2011
..IL-6 was the only elevated cytokine in the patients, and its level increased with a clear time course, indicating that IL-6 might be a prognostic marker for predicting the progression of plague...
- Comparative and evolutionary genomics of Yersinia pestis
Dongsheng Zhou
Laboratory of Analytical Microbiology, Institute of Microbiology and Epidemiology, National Center for Biomedical Analysis, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai, Beijing 100071, China
Microbes Infect 6:1226-34. 2004
..Understanding the genomic variability and the adaptive evolution of Y. pestis from the genomic point of view will contribute greatly to plague detection, identification, control and prevention...
- Insight into microevolution of Yersinia pestis by clustered regularly interspaced short palindromic repeats
Yujun Cui
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 3:e2652. 2008
..pestis from 26 natural plague foci of China, the former Soviet Union and Mongolia were analyzed, for validating CRISPR-based genotyping method and better understanding adaptive microevolution of Y. pestis...
- Histopathological observation of immunized rhesus macaques with plague vaccines after subcutaneous infection of Yersinia pestis
Guang Tian
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 6:e19260. 2011
..Thus, Chinese-origin rhesus macaques serve as useful models in studying Y. pestis pathogenesis, host response and the efficacy of new medical countermeasures against plague...
- Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay
Qing Qu
Department of Infectious Disease Control, Beijing Institute of Disease Control and Prevention, Beijing 100071, China
J Microbiol Methods 79:121-3. 2009
..0 x 10(3) to 3.9 x 10(5)CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection...
- A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates
Chen Chen
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing, China
PLoS ONE 2:e315. 2007
..However, the molecular mechanisms underlying STSS are poorly understood...
- Fur is a repressor of biofilm formation in Yersinia pestis
Fengjun Sun
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
PLoS ONE 7:e52392. 2012
....
- Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus
Fengjun Sun
Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, People s Republic of China
PLoS ONE 7:e44210. 2012
..Molecular regulation of target genes by AphA has been characterized in Vibrio harveyi and V. cholerae, but it is still poorly understood in V. parahaemolyticus...
- A genome sequence of novel SARS-CoV isolates: the genotype, GD-Ins29, leads to a hypothesis of viral transmission in South China
Xionglei He
Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China
Genomics Proteomics Bioinformatics 1:101-7. 2003
..It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development...
- Development of an up-converting phosphor technology-based 10-channel lateral flow assay for profiling antibodies against Yersinia pestis
Wenyan Hong
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No 20, Dongdajie, Fengtai District, Beijing 100071, People s Republic of China
J Microbiol Methods 83:133-40. 2010
..3% (44/51) and 66.7% (34/51) in patient sera, respectively. It is suggested that the TC-UPT-LF assay has been successfully developed for multi-detection and LcrV and YopD can be the potential diagnostic markers of the plague...
- Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus
Maofeng Qiu
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China
Clin Diagn Lab Immunol 12:474-6. 2005
....
- [Clustered regularly interspaced short palindromic repeats: structure, function and application--a review]
Yujun Cui
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
Wei Sheng Wu Xue Bao 48:1549-55. 2008
..We introduce the brief history', structure, function, bioinformatics research and application of this amazing immunity system in prokaryotic organism for inspiring more scientists to find their interest in this developing topic...
- Molecular and physiological insights into plague transmission, virulence and etiology
Dongsheng Zhou
State Key Laboratory of Pathogen and Biosecurity, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS, Beijing 100071, China
Microbes Infect 8:273-84. 2006
..The availability of multiple genomic sequences and more extensive use of genomics and proteomics technologies should allow a comprehensive dissection of the complex of host-adaptation and virulence in Y. pestis...
- Microarray analysis of temperature-induced transcriptome of Streptococcus suis serotype 2
Xiaotao Zeng
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China
Vector Borne Zoonotic Dis 11:215-21. 2011
..Our data provided a global profile of gene transcription induced by temperature alteration and shed light on some unforeseen lines for further pathogenesis investigation...
- Formation and regulation of Yersinia biofilms
Dongsheng Zhou
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
Protein Cell 2:173-9. 2011
..Pseudotuberculosis and Y. Pestis, but Y. Pestis has evolved changes in the regulation of biofilm development, thereby acquiring efficient arthropod-borne transmission...
- Interaction between Yersinia pestis and the host immune system
Bei Li
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China
Infect Immun 76:1804-11. 2008
- Reduced apoptosis of mouse macrophages induced by yscW mutant of Yersinia pestis results from the reduced secretion of YopJ and relates to caspase-3 signal pathway
Y Bi
Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China
Scand J Immunol 70:358-67. 2009
..This means although YscW does not induce apoptosis directly, it can indirectly affect apoptosis through reducing the secretion of YopJ...
- The cyclic AMP receptor protein, CRP, is required for both virulence and expression of the minimal CRP regulon in Yersinia pestis biovar microtus
Lingjun Zhan
Institute of Laboratory Animal Sciences, Chinese Academy of Medicine Peking Union Medical College, Beijing 100021, China
Infect Immun 76:5028-37. 2008
....
- The iron-responsive Fur regulon in Yersinia pestis
He Gao
College of Life Sciences, Northeast Normal University, Changchun 130024, People s Republic of China
J Bacteriol 190:3063-75. 2008
..The data presented here give us an overview of the iron-responsive Fur regulon in Y. pestis...
- Genotyping of hepatitis B virus (HBV) by oligonucleotides microarray
Yajun Song
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Fengtai District, Beijing, People's Republic of China
Mol Cell Probes 20:121-7. 2006
..The accuracy of genotyping was confirmed by analyzing DNA sequences of 24 samples. It is demonstrated that low-density oligo microarray can serve as a reliable and time-saving method to HBV genotyping from patient's sera...
- Identification of signature genes for rapid and specific characterization of Yersinia pestis
Dongsheng Zhou
National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Bejing, China
Microbiol Immunol 48:263-9. 2004
..pseudotuberculosis or a large collection of genomic DNAs from other organisms...
- Asymptomatic Yersinia pestis infection, China
Min Li
Emerg Infect Dis 11:1494-6. 2005
- Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans
Yajun Song
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, P. R. China
DNA Res 11:179-97. 2004
..brandti might have evolved from ancestral Y. pestis in a different lineage. The large genome fragment deletions in the 91001 chromosome and some pseudogenes may contribute to its unique nonpathogenicity to humans and host-specificity...
- Analysis of the three Yersinia pestis CRISPR loci provides new tools for phylogenetic studies and possibly for the investigation of ancient DNA
Gilles Vergnaud
Universite Paris Sud, Institut de Genetique et Microbiologie, Orsay, France
Adv Exp Med Biol 603:327-38. 2007
..In addition, the assay can be run in any laboratory. Interpretation of the data is not dependent on accurate sequence data...
