Ebenezer N Yamoah
Wang W, Flores M, Sihn C, Kim H, Zhang Y, Doyle K, et al
. Identification of a key residue in Kv7.1 potassium channel essential for sensing external potassium ions. J Gen Physiol. 2015;145:201-12 pubmed publisher
..The implications of our findings transcend the auditory system; mutations at this position also result in long QT syndrome in the heart. ..
Lu L, Sirish P, Zhang Z, Woltz R, Li N, Timofeyev V, et al
. Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3. J Biol Chem. 2015;290:4663-76 pubmed publisher
..Utilizing a Cav1.3 null mutant mouse model, we demonstrate that ablation of Cav1.3 results in a decrease in the protein expression of myosin light chain 2, which interacts and increases the membrane localization of SK2 channels. ..
Zhang X, Lee J, Lv P, Chen W, Kim H, Wei D, et al
. Etiology of distinct membrane excitability in pre- and posthearing auditory neurons relies on activity of Cl- channel TMEM16A. Proc Natl Acad Sci U S A. 2015;112:2575-80 pubmed publisher
Sihn C, Kim H, Woltz R, Yarov Yarovoy V, Yang P, Xu J, et al
. Mechanisms of Calmodulin Regulation of Different Isoforms of Kv7.4 K+ Channels. J Biol Chem. 2016;291:2499-509 pubmed publisher
..4 channels. This report provides mechanistic insights into the critical roles of Ca(2+)/CaM regulation of the Kv7.4 channel under physiological and pathological conditions. ..
Levic S, Yamoah E. Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos. Methods Mol Biol. 2016;1427:463-70 pubmed publisher
..Here, we describe the dissection technique for isolating the basilar papilla in developing chick inner ear. We also provide detailed examples of physiological (patch clamping) experiments using this preparation. ..
Lee J, Sihn C, Wang W, Flores C, Yamoah E. In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs). Methods Mol Biol. 2016;1427:513-23 pubmed publisher
..Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs. ..