Gene-expression profiling during curcumin-induced apoptosis reveals downregulation of CXCR4Joanna Skommer
Department of Clinical Sciences, University of Kuopio, Kuopio, Finland
Exp Hematol 35:84-95. 2007
..Based on our recent evidence that curcumin triggers cell demise in follicular lymphoma (FL) cells, we aimed to identify curcumin-regulated genes of utmost importance for the treatment of follicular lymphoma...
- Towards an understanding of apoptosis detection by SYTO dyes
Donald Wlodkowic
Institute of Clinical Sciences, Department of Clinical Microbiology, University of Kuopio, and Kuopio University Hospital, Finland
Cytometry A 71:61-72. 2007
..Herein, we attempt to provide further insights into mechanisms of reduced SYTO16 fluorescence during apoptosis...
- Multiparametric analysis of HA14-1-induced apoptosis in follicular lymphoma cells
Donald Wlodkowic
Department of Clinical Microbiology, University of Kuopio, Harjulantie 1 C, 70 211 Kuopio, Finland
Leuk Res 30:1187-92. 2006
..Overall, the study provides both mechanistic and clinically relevant information about the action of HA14-1...
SYTO probes in the cytometry of tumor cell deathDonald Wlodkowic
The Bioelectronics Research Center, University of Glasgow, Glasgow, United Kingdom
Cytometry A 73:496-507. 2008
..Finally, we provide a future outlook on SYTO probes in cytometric and microfluidics (Lab-on-a-Chip) high content analysis applications...
- Brefeldin A triggers apoptosis associated with mitochondrial breach and enhances HA14-1- and anti-Fas-mediated cell killing in follicular lymphoma cells
Donald Wlodkowic
Institute of Clinical Sciences, Department of Clinical Microbiology, University of Kuopio, Kuopio, Finland
Leuk Res 31:1687-700. 2007
....
- Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells
Donald Wlodkowic
The Bioelectronics Research Center, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT Glasgow, UK
Anal Chem 81:6952-9. 2009
....
- Cellular foundation of curcumin-induced apoptosis in follicular lymphoma cell lines
Joanna Skommer
Department of Clinical Microbiology, University of Kuopio, Finland
Exp Hematol 34:463-74. 2006
..In efforts to find novel approaches to inhibit proliferation and induce apoptosis in FL cells, we examined the action of naturally occurring compound curcumin in the three recently established FL cell lines...
- Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry
Donald Wlodkowic
Department of Electronics and Electrical Engineering, The Bioelectronics Research Center, University of Glasgow, Glasgow, UK
Exp Cell Res 315:1706-14. 2009
..In particular, it should allow for dynamic analysis of samples where low cell number has so far been an obstacle, e.g. primary cancer stems cells or circulating minimal residual tumors...
- Biological implications of polymeric microdevices for live cell assays
Donald Wlodkowic
Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, University of Glasgow, Glasgow, UK G12 8LT
Anal Chem 81:9828-33. 2009
..Results show that PDMS is nontoxic to cells, as their viability and growth characteristic in PDMS-based platforms is comparable to that of their polystyrene counterparts...
Cytometry in cell necrobiology revisited. Recent advances and new vistasDonald Wlodkowic
The Bioelectronics Research Centre, University of Glasgow, Glasgow, United Kingdom
Cytometry A 77:591-606. 2010
..This review highlights the key developments warranting a renaissance and evolution of cytometric techniques in the field of cell necrobiology...
- Microfluidic single-cell array cytometry for the analysis of tumor apoptosis
Donald Wlodkowic
The Bioelectronics Research Centre, Department of Electronics and Electrical Engineering, University of Glasgow, G12 8LT, United Kingdom
Anal Chem 81:5517-23. 2009
....
- Flow cytometry-based apoptosis detection
Donald Wlodkowic
The Bioelectronics Research Center, University of Glasgow, Rankine Building, Glasgow G12 8LT, UK
Methods Mol Biol 559:19-32. 2009
..In the present chapter, we outline a handful of commonly used methods that are based on the assessment of: mitochondrial transmembrane potential, activation of caspases, plasma membrane alterations and DNA fragmentation...
- HA14-1, a small molecule Bcl-2 antagonist, induces apoptosis and modulates action of selected anticancer drugs in follicular lymphoma B cells
Joanna Skommer
Department of Clinical Microbiology, University of Kuopio, Harjulantie 1 C, FIN 70211 Kuopio, Finland
Leuk Res 30:322-31. 2006
..Moreover, HA14-1 significantly enhanced dexamethasone- and doxorubicin-mediated (in schedule independent and dependent manner, respectively), but not vincristine-mediated cytotoxicity and apoptosis...
- Multiparameter detection of apoptosis using red-excitable SYTO probes
Donald Wlodkowic
Department of Biological Sciences, Glasgow Caledonian University, Glasgow, United Kingdom
Cytometry A 73:563-9. 2008
..Considering simplicity of staining protocols, low cost, and avoidance of spectral compensation problems, we expect that red-excitable SYTO dyes will find a wide range of cytometric applications...
- Larger than life: Mitochondria and the Bcl-2 family
Joanna Skommer
Department of Clinical Sciences, University of Kuopio, Harjulantie 1 C, 70211 Kuopio, Finland
Leuk Res 31:277-86. 2007
....
- Microfluidic cell arrays in tumor analysis: new prospects for integrated cytomics
Donald Wlodkowic
The Bioelectronics Research Centre, University of Glasgow, Rankine Building, Oakfiled Avenue, Glasgow, G12 8LT, UK
Expert Rev Mol Diagn 10:521-30. 2010
..Lab-on-a-chip microfluidic cell arrays therefore provide new opportunities for the development of content-rich personalized clinical diagnostics and higher-throughput clinical screening in oncology...
- Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells
Shannon L Faley
Bioelectronics Research Centre, University of Glasgow, Oakfield Avenue, Glasgow, UK G12 8LT
Lab Chip 9:2659-64. 2009
..Further studies show that dasatinib does, however, markedly reduce CML stem/progenitor cell migration in situ...
- SYTO probes: markers of apoptotic cell demise
Donald Wlodkowic
University of Glasgow, UK
Curr Protoc Cytom . 2007
....
- CXCR4 expression during tumour cell death
Joanna Skommer
Leuk Res 31:1155-6. 2007
