Design parameters to control synthetic gene expression in Escherichia coliMark Welch
DNA2 0, Menlo Park, CA, USA
PLoS ONE 4:e7002. 2009
..Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimentally supported design principles...
Designing genes for successful protein expressionMark Welch
DNA2 0, Inc, Suite A, Menlo Park, California, USA
Methods Enzymol 498:43-66. 2011
..Finally, we show how many of these methods can be accomplished using the synthetic biology software tool Gene Designer...
Engineering genes for predictable protein expressionClaes Gustafsson
DNA2 0 Inc, 1140 O Brien Drive, Menlo Park, CA 94025, USA
Protein Expr Purif 83:37-46. 2012
..We here discuss how these new tools are being applied and how they circumvent the constraints of previous approaches, highlighting some of the surprising and promising results emerging from the developing field of gene engineering...
You're one in a googol: optimizing genes for protein expressionMark Welch
DNA 2 0, Inc, 1430 O Brien Drive, Menlo Park, CA 94025, USA
J R Soc Interface 6:S467-76. 2009
....
In silico design of functional DNA constructsAlan Villalobos
DNA2 0, Inc, Menlo Park, CA, USA
Methods Mol Biol 852:197-213. 2012
..Together, the functions in Gene Designer provide a complete capability to design functional genetic constructs...
Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene clusterHugo G Menzella
Kosan Biosciences, Inc, 3832 Bay Center Place, Hayward, CA, 94545, USA
J Ind Microbiol Biotechnol 33:22-8. 2006
..Indeed, for optimal polyketide production, PKS expression had to be down-regulated by promoter attenuation to achieve balance with expression of the accessory proteins needed to support polyketide biosynthesis...