Viral shedding and immune responses to respiratory syncytial virus infection in older adultsEdward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, New York, USA
J Infect Dis 207:1424-32. 2013
..Comprehensive analyses of host, viral, and immune factors associated with severe respiratory syncytial virus (RSV) infection in adults have not been performed...
Can serum procalcitonin levels help interpret indeterminate chest radiographs in patients hospitalized with acute respiratory illness?Edward E Walsh
Department of Medicine, University of Rochester, Rochester, NY, USA
J Hosp Med 8:61-7. 2013
..Clinical diagnosis of pneumonia is difficult and chest radiographs often indeterminate, leading to incorrect diagnoses and antibiotic overuse...
Respiratory syncytial virus infection in adult populationsEdward E Walsh
University of Rochester School of Medicine and Dentistry, Rochester General Hospital, Rochester, NY, USA
Infect Disord Drug Targets 12:98-102. 2012
..Currently a vaccine for RSV is not available, and many challenges to developing a successful vaccine must be overcome...
- Respiratory syncytial virus infection in adults
Edward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA
Semin Respir Crit Care Med 32:423-32. 2011
..The greatest promise for reducing the impact of RSV in adults may be through immunization. However, an effective vaccine for RSV is not currently available...
- Sustained reduction in methicillin-resistant Staphylococcus aureus wound infections after cardiothoracic surgery
Edward E Walsh
Division of Infectious Diseases, University of Rochester School of Medicine and Dentistry and Rochester General Hospital, Rochester, NY 14621, USA
Arch Intern Med 171:68-73. 2011
..Methicillin-resistant Staphylococcus aureus (MRSA) wound infections after cardiac surgery have increased in recent years and carry significant morbidity and mortality. In our hospital, MRSA accounted for 56% of postoperative infections...
- Utility of serum procalcitonin values in patients with acute exacerbations of chronic obstructive pulmonary disease: a cautionary note
Ann R Falsey
Department of Medicine, University of Rochester, Rochester, NY, USA
Int J Chron Obstruct Pulmon Dis 7:127-35. 2012
..Therefore, we evaluated procalcitonin as a predictor of viral versus bacterial infection in patients hospitalized with AECOPD with and without evidence of pneumonia...
- Respiratory syncytial virus infection in elderly and high-risk adults
Ann R Falsey
Department of Medicine, Rochester General Hospital, Rochester, NY 14621, USA
N Engl J Med 352:1749-59. 2005
..Data on the epidemiology and clinical effects in community-dwelling elderly persons and high-risk adults can help in assessing the need for vaccine development...
- Detection of respiratory syncytial virus in adults with chronic obstructive pulmonary disease
Ann R Falsey
Infectious Diseases Unit, Department of Medicine, Rochester General Hospital, 1425 Portland Avenue, Rochester, NY 14621, USA
Am J Respir Crit Care Med 173:639-43. 2006
..These data raise the possibility of persistent low-grade infection in this population, which could have implications in COPD pathogenesis...
- Factors associated with symptomatic rhinovirus infection in patients with COPD
Anurag Gandhi
University of Rochester, Rochester, NY, USA
J Clin Virol 55:343-7. 2012
..Human rhinoviruses (HRV) can be detected by RT-PCR in a large proportion of acute exacerbations of chronic obstructive pulmonary disease (AECOPD) but can also be detected in COPD patients without symptoms...
- Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection
F Eun Hyung Lee
David H Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
J Infect Dis 195:1779-88. 2007
..Illness severity is likely multifactorial; however, unlike other viral infections, both type 1 and type 2 cytokine responses have been implicated in severe disease...
- Humoral immunity to human metapneumovirus infection in adults
Ann R Falsey
Department of Medicine, University of Rochester School of Medicine and Dentistry, United States
Vaccine 28:1477-80. 2010
..Serum antibody may play a role in protection from hMPV infection supporting the development of an hMPV vaccine that stimulates humoral immunity...
- Pulmonary complications of interpandemic influenza A in hospitalized adults
Yoshihiko Murata
Department of Medicine, University of Rochester School of Medicine and Dentistry and Infectious Diseases Unit, Department of Medicine, Rochester General Hospital, Rochester, NY 14621, USA
J Infect Dis 195:1029-37. 2007
....
- Impact of rapid diagnosis on management of adults hospitalized with influenza
Ann R Falsey
Departments of Medicine, Rochester General Hospital and University of Rochester School of Medicine and Dentistry, Rochester, NY 14621, USA
Arch Intern Med 167:354-60. 2007
..We compared the clinical management of patients with influenza whose rapid antigen test result was positive (Ag+) with the management of those whose rapid antigen test result was negative or the test was not performed (Ag0)...
- Yield of sputum for viral detection by reverse transcriptase PCR in adults hospitalized with respiratory illness
Ann R Falsey
University of Rochestera and Rochester General Hospital, b Rochester, New York, USA
J Clin Microbiol 50:21-4. 2012
..Further studies designed to adapt the use of sputum samples for commercial RT-PCR respiratory virus assays are needed...
- Circulating antibody-secreting cells during acute respiratory syncytial virus infection in adults
F Eun Hyung Lee
Division of Pulmonary and Critical Care Medicine, University of Rochester and Rochester General Hospital, Rochester, New York, USA
J Infect Dis 202:1659-66. 2010
..The specificity and duration of circulating human antibody-secreting cells (ASCs) after vaccination have been well described, but characteristics of ASCs during acute respiratory infections have not been well studied...
- Circulating human antibody-secreting cells during vaccinations and respiratory viral infections are characterized by high specificity and lack of bystander effect
F Eun Hyung Lee
Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
J Immunol 186:5514-21. 2011
..Additionally, high specificity of circulating ASCs after antigenic challenge highlights the diagnostic value of interrogating ASCs as an ideal single-time-point diagnostic immune surrogate for serology during acute infection...
- Is clinical recognition of respiratory syncytial virus infection in hospitalized elderly and high-risk adults possible?
Edward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14621, USA
J Infect Dis 195:1046-51. 2007
..The clinical and radiographic features of respiratory syncytial virus (RSV) infection in elderly hospitalized persons have not been described in detail, to our knowledge, despite its relative frequent occurrence...
- Humoral response to the central unglycosylated region of the respiratory syncytial virus attachment protein
Yoshihiko Murata
Division of Infectious Diseases, Department of Medicine, University of Rochester School of Medicine and Dentistry and Infectious Diseases Unit, Rochester General Hospital, 601 Elmwood Avenue Box 689, Rochester, NY 14642, USA Yoshihiko
Vaccine 28:6242-6. 2010
..Our results have implications for RSV G-based serological diagnostics and vaccine development...
- Detection of respiratory syncytial virus and human metapneumovirus by reverse transcription polymerase chain reaction in adults with and without respiratory illness
Ann R Falsey
Department of Medicine, Infectious Diseases Unit, Rochester General Hospital, 1425 Portland Avenue, Rochester, NY 14621, USA
J Clin Virol 35:46-50. 2006
..No subject had a positive swab on days 14, 21 or 28. CONCLUSION: Asymptomatic carriage of RSV or hMPV is uncommon. RT-PCR should be a useful method for the diagnosis of these viral illnesses in adults...
- Humoral and mucosal immunity in protection from natural respiratory syncytial virus infection in adults
Edward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, and Department of Medicine, Rochester General Hospital, Rochester, New York, USA
J Infect Dis 190:373-8. 2004
..Multivariate analysis found that low RSV-specific nasal IgA was an independently significant risk factor for RSV infection...
- The effect of steroid use in hospitalized adults with respiratory syncytial virus-related illness
F Eun Hyung Lee
Division of Pulmonary and Critical Care Medicine, University of Rochester School of Medicine and Dentistry, NY, USA
Chest 140:1155-61. 2011
..The effects of short-term high-dose steroid treatment on viral load and adaptive immunity to RSV have not been examined in adults...
- Risk factors for respiratory failure associated with respiratory syncytial virus infection in adults
Coley B Duncan
Department of Medicine, Rochester General Hospital, Rochester, New York 14621 3001, USA
J Infect Dis 200:1242-6. 2009
..7 +/- 1.7 plaque-forming units (PFUs)/mL vs 2.4 +/- 1.1 PFUs/mL, P = .02), and high viral load was independently associated with respiratory failure...
- Age related differences in humoral immune response to respiratory syncytial virus infection in adults
Edward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
J Med Virol 73:295-9. 2004
..The relationship of immune response to disease pathogenesis in older persons deserves further study...
- Identification of and human serum reactogenicity to neutralizing epitopes within the central unglycosylated region of the respiratory syncytial virus attachment protein
Yoshihiko Murata
Division of Infectious Diseases, Department of Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 689, Rochester, NY 14642, USA
Clin Vaccine Immunol 17:695-7. 2010
..In approximately 40% of hospitalized and outpatient adults infected with RSV subtype A, these contiguous residues are the target of > or =4-fold increases in IgG response between acute- and convalescent-phase sera...
- Comparison of quantitative reverse transcription-PCR to viral culture for assessment of respiratory syncytial virus shedding
Ann R Falsey
Department of Medicine Infectious Disease Unit, Rochester General Hospital, University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA
J Clin Microbiol 41:4160-5. 2003
..Quantitative RT-PCR has an excellent correlation with viral titers, as measured by culture, and should be a useful tool for future studies addressing viral load and disease pathogenesis...
- Viral pneumonia in older adults
Ann R Falsey
Department of Medicine, Rochester General Hospital, Rochester, NY 14621, USA
Clin Infect Dis 42:518-24. 2006
..Because clinical features and periods of activity for many viruses overlap, laboratory confirmation of influenza is recommended for cases involving seriously ill or institutionalized patients...
- Experimental infection of humans with A2 respiratory syncytial virus
F Eun-Hyung Lee
Department of Medicine, University of Rochester School of Medicine and Dentistry, Box 689, 601 Elmwood Avenue, Rochester, NY 14642, USA
Antiviral Res 63:191-6. 2004
..This model provides a safe and efficient mechanism for proof-of-concept studies of anti-viral agents and RSV vaccines, as well as for investigation of immune responses to infection...
- Microneutralization assay for the measurement of neutralizing antibodies to human metapneumovirus
Ann R Falsey
Department of Medicine, Rochester General Hospital, Rochester, NY 14621 3001, USA
J Clin Virol 46:314-7. 2009
..Human metapneumovirus (hMPV) is a newly discovered virus which causes respiratory illness in persons of all ages...
- Risk factors for severe respiratory syncytial virus infection in elderly persons
Edward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
J Infect Dis 189:233-8. 2004
..quot; These data suggest that the induction of neutralizing serum antibody with an RSV vaccine may potentially reduce disease severity in adult populations...
- Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults
Aida E Casiano-Colón
ViaHealth Clinical Laboratories and Pathology, Rochester, NY, USA
J Clin Virol 28:169-74. 2003
..DFA detected 14 (23%), VIDAS detected 12 (20%) and BD detected only 6 (10%) of the RSV infected persons. Rapid antigen detection tests for the diagnosis of RSV respiratory illness in adults are of limited value...
- Antigenic presentation of heterologous epitopes engineered into the outer surface-exposed helix 4 loop region of human papillomavirus L1 capsomeres
Yoshihiko Murata
Division of Infectious Diseases, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Virol J 6:81. 2009
..We sought to utilize HPV L1 capsomeres as a vaccine platform by engineering heterologous epitopes within L1 derivatives deleted for h4 domain...
- The balance between influenza- and RSV-specific CD4 T cells secreting IL-10 or IFNgamma in young and healthy-elderly subjects
F Eun Hyung Lee
David H Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
Mech Ageing Dev 126:1223-9. 2005
..A similar trend was seen for influenza. IFNgamma-secreting CD4 T cells contributed significantly more to anti-RSV than anti-influenza responses in both groups...
- Peak frequencies of circulating human influenza-specific antibody secreting cells correlate with serum antibody response after immunization
Jessica L Halliley
Division of Pulmonary and Critical Care Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
Vaccine 28:3582-7. 2010
..The kinetics of this response and the relationship of circulating ASCs to protective antibody titers have not been completely explored...
- Human metapneumovirus infections in adults: another piece of the puzzle
Edward E Walsh
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Arch Intern Med 168:2489-96. 2008
..We describe the incidence and clinical impact of human metapneumovirus (HMPV), a newly identified virus, in adults...
- Human metapneumovirus infections in young and elderly adults
Ann R Falsey
Infectious Diseases Unit, Rochester General Hospital, University of Rochester School of Medicine and Dentistry, 1425 Portland Avenue, Rochester, NY 14621, USA
J Infect Dis 187:785-90. 2003
..In conclusion, hMPV infection occurs in adults of all ages and may account for a significant portion of persons hospitalized with respiratory infections during some years...
- Respiratory syncytial virus infection in elderly adults
Ann R Falsey
Department of Medicine, University of Rochester School of Medicine and Dentistry, and Rochester General Hospital, Rochester, New York, USA
Drugs Aging 22:577-87. 2005
..An effective RSV vaccine has not yet been developed. Therefore, prevention of RSV is limited to standard infection control practices, such as hand washing and the use of gowns and gloves...
- Serum antibody decay in adults following natural respiratory syncytial virus infection
Ann R Falsey
Department of Medicine at Rochester General Hospital, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA
J Med Virol 78:1493-7. 2006
..In contrast, titers of uninfected subjects were relatively stable. The partial immunity resulting from a boost in serum antibody following natural RSV infection in adults appears to be short lived...
- Comparison of the safety and immunogenicity of 2 respiratory syncytial virus (rsv) vaccines--nonadjuvanted vaccine or vaccine adjuvanted with alum--given concomitantly with influenza vaccine to high-risk elderly individuals
Ann R Falsey
Department of Medicine, Rochester General Hospital, and University of Rochester School of Medicine, Rochester, NY 14621, USA
J Infect Dis 198:1317-26. 2008
..Respiratory syncytial virus (RSV) has been recognized recently as an important adult pathogen...
- Rhinovirus and coronavirus infection-associated hospitalizations among older adults
Ann R Falsey
Division of Infectious Diseases and Department of Medicine, University of Rochester School of Medicine and Dentistry, and Rochester General Hospital, Rochester, New York 14621, USA
J Infect Dis 185:1338-41. 2002
..These data suggest that rhinoviruses and coronaviruses may be associated with serious respiratory illnesses in frail older adults...
- Novel coronavirus and severe acute respiratory syndrome
Ann R Falsey
Rochester General Hospital, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
Lancet 361:1312-3. 2003
- Clinical features of influenza A virus infection in older hospitalized persons
Edward E Walsh
Division of Infectious Diseases and Department of Medicine, and Department of Biostatistics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA
J Am Geriatr Soc 50:1498-503. 2002
..To determine the value of clinical parameters in predicting influenza in older persons and those with underlying cardiopulmonary conditions hospitalized with respiratory illnesses...
- Diagnosis of respiratory syncytial virus infection: comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness
Ann R Falsey
Department of Medicine, Rochester General Hospital, University of Rochester School of Medicine and Dentistry, Rochester, New York 14621, USA
J Clin Microbiol 40:817-20. 2002
..The overall RT-PCR sensitivity was 73%, and specificity was 99%. These data indicate that RT-PCR is an excellent method for the diagnosis of acute RSV infection in adults...
