Matthew K Waldor

Summary

Publications

  1. Hubbard T, D Gama J, Billings G, Davis B, Waldor M. Unsupervised Learning Approach for Comparing Multiple Transposon Insertion Sequencing Studies. mSphere. 2019;4: pubmed publisher
    ..Our findings suggest that PCA-based analytics will be a straightforward widely applicable approach for comparing diverse transposon insertion sequencing screens. ..
  2. Park J, Helble J, Lazarus J, Yang G, Blondel C, Doench J, et al. A FACS-Based Genome-wide CRISPR Screen Reveals a Requirement for COPI in Chlamydia trachomatis Invasion. iScience. 2019;11:71-84 pubmed publisher
    ..Together, our findings establish the requirement for COPI in C. trachomatis invasion and the utility of FACS-based CRISPR screening for the elucidation of host factors required for pathogen invasion. ..
  3. Möll A, Dörr T, Alvarez L, Chao M, Davis B, Cava F, et al. Cell separation in Vibrio cholerae is mediated by a single amidase whose action is modulated by two nonredundant activators. J Bacteriol. 2014;196:3937-48 pubmed publisher
    ..coli and V. cholerae, they can be combined into diverse functional regulatory networks. ..
  4. Pacheco A, Lazarus J, Sit B, Schmieder S, Lencer W, Blondel C, et al. CRISPR Screen Reveals that EHEC's T3SS and Shiga Toxin Rely on Shared Host Factors for Infection. MBio. 2018;9: pubmed publisher
    ..Since antibiotics are contraindicated for the treatment of EHEC, therapeutics targeting sphingolipid biosynthesis are a promising alternative, as they could provide protection against both of the pathogen's key virulence factors. ..
  5. Livny J, Zhou X, Mandlik A, Hubbard T, Davis B, Waldor M. Comparative RNA-Seq based dissection of the regulatory networks and environmental stimuli underlying Vibrio parahaemolyticus gene expression during infection. Nucleic Acids Res. 2014;42:12212-23 pubmed publisher
    ..cholerae, likely due to the distinct ways in which these diarrheal pathogens interact with and modulate the environment in the small intestine. ..
  6. Gerding M, Chao M, Davis B, Waldor M. Molecular Dissection of the Essential Features of the Origin of Replication of the Second Vibrio cholerae Chromosome. MBio. 2015;6:e00973 pubmed publisher
    ..Finally, our findings suggest that the OriSeq approach is likely to be widely applicable for defining critical bases in cis-acting sequences. ..
  7. Dörr T, Alvarez L, Delgado F, Davis B, Cava F, Waldor M. A cell wall damage response mediated by a sensor kinase/response regulator pair enables beta-lactam tolerance. Proc Natl Acad Sci U S A. 2016;113:404-9 pubmed publisher
    ..Taken together, these findings implicate WigKR as a regulator of cell wall synthesis that controls cell wall homeostasis in response to antibiotics and likely during normal growth as well. ..
  8. Hubbard T, Chao M, Abel S, Blondel C, Abel Zur Wiesch P, Zhou X, et al. Genetic analysis of Vibrio parahaemolyticus intestinal colonization. Proc Natl Acad Sci U S A. 2016;113:6283-8 pubmed publisher
    ..Thus, disparate horizontally acquired virulence systems have been placed under the control of this ancestral transcription factor across independently evolved human pathogens. ..
  9. Blondel C, Park J, Hubbard T, Pacheco A, Kuehl C, Walsh M, et al. CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity. Cell Host Microbe. 2016;20:226-37 pubmed publisher
    ..parahaemolyticus, and highlight the utility of genome-wide CRISPR/Cas9 screens to discover processes underlying host-pathogen interactions. ..

More Information

Publications14

  1. Hubbard T, Billings G, Dörr T, Sit B, Warr A, Kuehl C, et al. A live vaccine rapidly protects against cholera in an infant rabbit model. Sci Transl Med. 2018;10: pubmed publisher
    ..Mathematical modeling indicates that an intervention that works at the speed of HaitiV-mediated protection could improve the public health impact of reactive vaccination. ..
  2. Fu Y, Waldor M, Mekalanos J. Tn-Seq analysis of Vibrio cholerae intestinal colonization reveals a role for T6SS-mediated antibacterial activity in the host. Cell Host Microbe. 2013;14:652-63 pubmed publisher
    ..cholerae T6SS is functional and mediates antagonistic interbacterial interactions during infection. ..
  3. Zhou X, Massol R, Nakamura F, Chen X, Gewurz B, Davis B, et al. Remodeling of the intestinal brush border underlies adhesion and virulence of an enteric pathogen. MBio. 2014;5: pubmed publisher
    ..Our findings suggest a new role for filamin in brush border maintenance and raise the possibility that microvillus effacement is a common strategy among enteric pathogens for enhancing adhesion to host cells. ..
  4. Kimura S, Hubbard T, Davis B, Waldor M. The Nucleoid Binding Protein H-NS Biases Genome-Wide Transposon Insertion Landscapes. MBio. 2016;7: pubmed publisher
    ..g., DNA binding proteins such as H-NS) can systematically modulate the frequency of transposon insertions in TIS studies and add a note of caution for interpretation of TIS data. ..
  5. Zhang T, Abel S, Abel Zur Wiesch P, Sasabe J, Davis B, Higgins D, et al. Deciphering the landscape of host barriers to Listeria monocytogenes infection. Proc Natl Acad Sci U S A. 2017;114:6334-6339 pubmed publisher
    ..monocytogenes from seeding these organs. Collectively, these observations illustrate the potency of the STAMP approach to decipher the impact of host factors on population dynamics of pathogens during infection. ..