An early PEEP/FIO2 trial identifies different degrees of lung injury in patients with acute respiratory distress syndromeJesus Villar
Multidisciplinary Organ Dysfunction Evaluation Research Network, Hospital Universitario Dr Negrin, Las Palmas de Gran Canaria, Canary Islands, Spain
Am J Respir Crit Care Med 176:795-804. 2007
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A universal definition of ARDS: the PaO2/FiO2 ratio under a standard ventilatory setting--a prospective, multicenter validation studyJesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Intensive Care Med 39:583-92. 2013
..We hypothesized that the use of PaO2/FiO2 calculated under a standard ventilatory setting within 24 h of ARDS diagnosis allows a more clinically relevant ARDS classification...
- The American-European Consensus Conference definition of the acute respiratory distress syndrome is dead, long live positive end-expiratory pressure!
J Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario Dr Negrin, Las Palmas de Gran Canaria, Spain Electronic address
Med Intensiva 36:571-5. 2012
..In this Point of View, we review the history of the definition of ARDS and discussed the methodological concerns regarding adopting this new, revised ARDS definition...
A quality assessment of genetic association studies supporting susceptibility and outcome in acute lung injuryCarlos Flores
CIBER de Enfermedades Respiratorias Instituto de Salud Carlos III, Carretera Soller km 12, 07110 Mallorca, Spain
Crit Care 12:R130. 2008
..We aimed to assess the quality of positive genetic association studies with ALI susceptibility and/or outcome in adults in order to highlight their consistency and major limitations...
- Incidence, organ dysfunction and mortality in severe sepsis: a Spanish multicentre study
Jesus Blanco
Critical Care Department, Nuevo Hospital Universitario Río Hortega, Calle Dulzaina s n, 47012 Valladolid, Spain
Crit Care 12:R158. 2008
..We present the first extensive dataset on the epidemiology of severe sepsis treated in ICUs in Spain...
- Ventilator-induced lung injury and sepsis: two sides of the same coin?
J Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Barranco de la Ballena s n, Las Palmas de Gran Canaria, Spain
Minerva Anestesiol 77:647-53. 2011
..In this line of thought, we hypothesize that injurious mechanical ventilation could be responsible for the perpetuation and worsening of sepsis in some patients and for the development of a sepsis-like syndrome in others...
- A CXCL2 polymorphism is associated with better outcomes in patients with severe sepsis
Jesus Villar
Multidisciplinary Organ Dysfunction Evaluation Research Network, Hospital Universitario Dr Negrin, Las Palmas de Gran Canaria, Canary Islands, Spain
Crit Care Med 35:2292-7. 2007
..We hypothesized that a tandem repeat polymorphism (AC)n in the CXCL2 gene, previously associated with susceptibility to severe sepsis, contributes to morbidity and mortality in severe sepsis...
- Evaluating an educational intervention to improve the accuracy of death certification among trainees from various specialties
Jesus Villar
Multidisciplinary Organ Dysfunction Evaluation Research Network MODERN, Research Unit, Hospital Universitario Dr, Negrin, Las Palmas de Gran Canaria, Spain
BMC Health Serv Res 7:183. 2007
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- Angiotensin-converting enzyme insertion/deletion polymorphism is not associated with susceptibility and outcome in sepsis and acute respiratory distress syndrome
Jesus Villar
Multidisciplinary Organ Dysfunction Evaluation Research Network MODERN, Research Unit, Hospital Universitario Dr Negrin, Barranco de la Ballena, Las Palmas de Gran Canaria, Canary Islands, Spain
Intensive Care Med 34:488-95. 2008
..We hypothesized that the I/D polymorphism also confers susceptibility to sepsis and is a predisposing factor for morbidity and mortality of patients with severe sepsis...
- Genetic determinants of survival in sepsis and acute lung injury
J Villar
Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario Dr. Negrin, Las Palmas de Gran Canaria, Spain
Minerva Anestesiol 74:341-5. 2008
- The ALIEN study: incidence and outcome of acute respiratory distress syndrome in the era of lung protective ventilation
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Intensive Care Med 37:1932-41. 2011
..The goal of this study was to examine ARDS incidence and outcome under current lung protective ventilatory support practices before and after the diagnosis of ARDS...
- Mechanical ventilation modulates Toll-like receptor signaling pathway in a sepsis-induced lung injury model
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Intensive Care Med 36:1049-57. 2010
..How these two responses interact when present simultaneously remains to be elucidated. We hypothesized that MV modulates innate host response during sepsis by influencing Toll-like receptor (TLR) signaling...
- Experimental ventilator-induced lung injury: exacerbation by positive end-expiratory pressure
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Anesthesiology 110:1341-7. 2009
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- Activation of the Wnt/β-catenin signaling pathway by mechanical ventilation is associated with ventilator-induced pulmonary fibrosis in healthy lungs
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
PLoS ONE 6:e23914. 2011
..Since activation of Wnt/β-catenin signaling has been suggested to be central to mechanisms of lung healing and fibrosis, we hypothesized that the Wnt/β-catenin signaling plays a role during VILI...
- Is acute respiratory distress syndrome an iatrogenic disease?
Jesus Villar
Crit Care 14:120. 2010
..We examine these interesting results after providing a brief historical perspective and discuss the limitations and implications of the study...
- Acute respiratory distress syndrome definition: do we need a change?
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Curr Opin Crit Care 17:13-7. 2011
..The purpose of this review is to examine the current definition for ARDS and to discuss why this definition may not be the most appropriate definition for this syndrome...
- A risk tertiles model for predicting mortality in patients with acute respiratory distress syndrome: age, plateau pressure, and P(aO(2))/F(IO(2)) at ARDS onset can predict mortality
Jesus Villar
Centro de Investigación Biomédica en Red CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Respir Care 56:420-8. 2011
..We examined whether stratification by tertiles of respiratory and ventilatory variables at the onset of acute respiratory distress syndrome (ARDS) identifies patients with different risks of death in the intensive care unit...
- Mechanical ventilation modulates TLR4 and IRAK-3 in a non-infectious, ventilator-induced lung injury model
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Spain
Respir Res 11:27. 2010
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- What is the acute respiratory distress syndrome?
Jesus Villar
Centro de Investigación Biomédica en Red CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Respir Care 56:1539-45. 2011
..This paper examines our current understanding of ARDS and discusses why the current definition may not be the most appropriate for research and clinical practice...
- A common haplotype of the LBP gene predisposes to severe sepsis
Carlos Flores
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Spain
Crit Care Med 37:2759-66. 2009
..The lipopolysaccharide-binding protein is an acute-phase protein that plays a dominant role in the genesis of sepsis by initiating signal transduction pathways leading to the activation of the inflammatory host response...
- Positive end-expiratory pressure modulates local and systemic inflammatory responses in a sepsis-induced lung injury model
Maria Teresa Herrera
Research Institute, Hospital Universitario N S de Candelaria, Carretera del Rosario s n, 38010, Santa Cruz de Tenerife, Canary Islands, Spain
Intensive Care Med 29:1345-53. 2003
..We examined the effects of positive end-expiratory pressure (PEEP) in the setting of a clinically relevant, in vivo animal model of sepsis-induced acute lung injury ventilated with low or injurious tidal volume...
- Interleukin-1 receptor-associated kinase 3 gene associates with susceptibility to acute lung injury
María Pino-Yanes
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Am J Respir Cell Mol Biol 45:740-5. 2011
..017). Despite the need for replication in independent studies, our data suggest that common SNPs in the IRAK3 gene may be determinants of sepsis-induced ALI...
- Serum lipopolysaccharide binding protein levels predict severity of lung injury and mortality in patients with severe sepsis
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
PLoS ONE 4:e6818. 2009
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- No association between genetic ancestry and susceptibility to asthma or atopy in Canary Islanders
María Pino-Yanes
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
Immunogenetics 64:705-11. 2012
..Larger studies, examining more genetic variants, would be needed to explore such possibility...
- WNT/β-catenin signaling is modulated by mechanical ventilation in an experimental model of acute lung injury
Jesus Villar
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain, Jesús
Intensive Care Med 37:1201-9. 2011
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- Bench-to-bedside review: understanding genetic predisposition to sepsis
Jesus Villar
Division Critical Care Research, Research Institute, Hospital Universitario NS de Candelaria, Tenerife, Canary Islands, Spain
Crit Care 8:180-9. 2004
..We envision a time in the near future when genotyping will be include in the standard evaluation of critically ill patients and will help to prioritize a therapeutic option...
- Injurious mechanical ventilation affects neuronal activation in ventilated rats
María Elisa Quilez
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, C Sinesio Delgado 6, Madrid, Spain
Crit Care 15:R124. 2011
..We aimed to investigate the effect of the increase of tidal volume (Vt) on brain activation in a rat model...
- A high positive end-expiratory pressure, low tidal volume ventilatory strategy improves outcome in persistent acute respiratory distress syndrome: a randomized, controlled trial
Jesus Villar
Canarian Institute for Biomedical Research, Tomas Morales 6 1, 35003 Las Palmas de Gran Canaria, Canary Islands, Spain
Crit Care Med 34:1311-8. 2006
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- Genetic susceptibility to acute lung injury
Jesus Villar
Research Institute, Hospital Universitario N S de Candelaria, Tenerife, Canary Islands, Spain
Crit Care Med 31:S272-5. 2003
..These may affect the responses of critically ill patients to acute lung injury and acute respiratory distress syndrome and may affect outcome...
- Common variants of TLR1 associate with organ dysfunction and sustained pro-inflammatory responses during sepsis
María Pino-Yanes
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
PLoS ONE 5:e13759. 2010
..We examined the association of common variants of TLR1 gene with sepsis-derived complications in an independent study and with serum levels for four inflammatory biomarkers among septic patients...
- Why are physicians so skeptical about positive randomized controlled clinical trials in critical care medicine?
Jesus Villar
Research Institute, Hospital Universitario N S de Candelaria, Carretera del Rosario s n, Canary Islands, 38010, Santa Cruz de Tenerife, Spain
Intensive Care Med 31:196-204. 2005
- Searching for novel molecular targets of chronic rejection in an orthotopic experimental lung transplantation model
Norberto Santana-Rodríguez
Research Unit, Experimental Surgery, Hospital Dr Negrin, Las Palmas de Gran Canaria, Spain
J Heart Lung Transplant 31:213-21. 2012
..There remains no effective treatment for CR. The aim of this study was to identify new molecular mechanisms involved in CR by using DNA microarray analysis...
- North African influences and potential bias in case-control association studies in the Spanish population
María Pino-Yanes
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
PLoS ONE 6:e18389. 2011
..Using ancestry informative markers (AIMs), we aimed to measure the African influences in Spanish populations and to explore whether these might introduce statistical bias in population-based association studies...
- Characterization of the first VanB vancomycin-resistant Enterococcus faecium isolated in a Spanish hospital
Fabián Lorenzo-Díaz
Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, Spain
Curr Microbiol 48:199-203. 2004
..Sequence analysis of the vanB gene showed that this isolate belonged to subtype vanB2. Moreover, PCR amplification analysis indicated that the vanB gene cluster was linked to a Tn5382-like transposon...
- A CXCL2 tandem repeat promoter polymorphism is associated with susceptibility to severe sepsis in the Spanish population
C Flores
Research Institute Research Center associated to Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain, Hospital Universitario NS de Candelaria, Tenerife, Spain
Genes Immun 7:141-9. 2006
..22-4.03; P=0.008) and after a genomic control adjustment (P=0.017). Although replicate studies and functional assays are needed, these results suggest that CXCL2 gene variants may contribute to the development of severe sepsis...
- Genetics and the pathogenesis of adult respiratory distress syndrome
Jesus Villar
Research Institute, Hospital de la Candelaria, Tenerife, Canary Islands, Spain
Curr Opin Crit Care 8:1-5. 2002
..This article reviews some of those susceptibility factors for critical illness and acute lung injury...
- Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter-isolated Staphylococcus
Juana V Martín-López
Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, 38010 Santa Cruz de Tenerife, Spain
Int Microbiol 7:63-6. 2004
..Furthermore, the method allows differential detection of the ica locus from Staphylococcus aureus and Staphylococcus epidermidis...
- Molecular cloning and expression in yeast of caprine prochymosin
María C Vega-Hernández
Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, 38010, Santa Cruz de Tenerife, Spain
J Biotechnol 114:69-79. 2004
..cerevisiae culture supernatants by affinity chromatography. Proteolytic activity assayed toward casein fractions indicated that the recombinant caprine chymosin specifically hydrolysed kappa-casein...
- Pulsed ultrasounds accelerate healing of rib fractures in an experimental animal model: an effective new thoracic therapy?
Norberto Santana-Rodríguez
Experimental Surgery Unit, Research Unit, Hospital Universitario Dr Negrin, Las Palmas de Gran Canaria, Spain
J Thorac Cardiovasc Surg 141:1253-8. 2011
..Since it has been reported that pulsed ultrasounds accelerates repair of limb fractures, we hypothesized that the application of pulsed ultrasounds will modify the course of healing in an animal model of rib fracture...
- Heat shock proteins and ventilator-induced lung injury
Jesus Villar
Research Institute, Hospital Universitario N S de Candelaria, and Department of Microbiology, University of La Laguna, Tenerife, Canary Islands, Spain
Curr Opin Crit Care 9:9-14. 2003
..Several studies support the hypothesis that HSPs are cytoprotective In addition, recent investigations have demonstrated that HSP70 is released into the systemic circulation and is involved in the activation of innate immunity...
- Current definitions of acute lung injury and the acute respiratory distress syndrome do not reflect their true severity and outcome
J Villar
Research Institute, Hospital de la Candelaria, Tenerife, Canary Islands, Spain
Intensive Care Med 25:930-5. 1999
..However, this definition may not reflect the true prevalence, severity and prognosis of these syndromes...
- Genetic susceptibility to respiratory diseases
J Villar
Research Institute, Hospital N S de Candelaria, Carretera del Rosario, S N, 38010 Santa Cruz de Tenerife, Canary Islands, Spain
Minerva Anestesiol 67:193-7. 2001
..The author reviews some of these complex interactions suggesting that even the inflammatory response during lung injury can be influenced by genetic polymorphisms...
- Anti-inflammatory activity of a novel family of aryl ureas compounds in an endotoxin-induced airway epithelial cell injury model
Nuria E Cabrera-Benítez
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain
PLoS ONE 7:e48468. 2012
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- Asynchrony, neural drive, ventilatory variability and COMFORT: NAVA versus pressure support in pediatric patients. A non-randomized cross-over trial
Pedro de la Oliva
Pediatric Intensive Care Unit UCIP, Hospital Universitario Materno Infantil La Paz, Paseo de la Castellana, 261, 28046, Madrid, Spain
Intensive Care Med 38:838-46. 2012
..To determine if neurally adjusted ventilatory assist (NAVA) improves asynchrony, ventilatory drive, breath-to-breath variability and COMFORT score when compared to pressure support (PS)...
- Effects of induced hypothermia in patients with septic adult respiratory distress syndrome
J Villar
Intensive Care Unit, Hospital del Pino, Las Palmas, Canary Islands, Spain
Resuscitation 26:183-92. 1993
..30 +/- 6%, P < 0.05). CONCLUSIONS: This study suggests that hypothermia was effective in improving oxygenation and survival in patients with severe ARDS associated with sepsis, even though VO2 was unchanged...
- Ventilator or physician-induced lung injury?
J Villar
Research Institute, Hospital Universitario N S of Candelaria, Tenerife, Canary Islands, Spain
Minerva Anestesiol 71:255-8. 2005
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- The use of positive end-expiratory pressure in the management of the acute respiratory distress syndrome
J Villar
Research Institute, Hospital Universitario N S de Candelaria, Tenerife, Canary Islands, Spain
Minerva Anestesiol 71:265-72. 2005
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- From ventilator-induced lung injury to physician-induced lung injury: why the reluctance to use small tidal volumes?
J Villar
Hospital Universitario N S de Candelaria, Tenerife, Canary Islands, Spain
Acta Anaesthesiol Scand 48:267-71. 2004
- Low vs high positive end-expiratory pressure in the ventilatory management of acute lung injury
J Villar
Division of Organ Dysfunction Research Instituto Canario de Investigación Biomédica ICIB, Las Palmas de Gran Canaria and Santa Cruz de Tenerife, Canary Islands, Spain
Minerva Anestesiol 72:357-62. 2006
..In this article I will review the basic mechanisms of PEEP and the current knowledge of the effects of PEEP on the evolution and outcome of ALI/ARDS...
