Carlos Sevcik

Summary

Country: Venezuela

Publications

  1. ncbi Identification of Enterobacter bacteria as saxitoxin producers in cattle's rumen and surface water from Venezuelan Savannahs
    C Sevcik
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas, Apartado 21827, Caracas1020A, Venezuela
    Toxicon 42:359-66. 2003
  2. ncbi Initial volume of a drug before it reaches the volume of distribution: pharmacokinetics of F(ab')2 antivenoms and other drugs
    Carlos Sevcik
    Laboratory of Cellular Neuropharmacology, Apartado 21827, Caracas 1020A, Venezuela
    Toxicon 50:653-65. 2007
  3. doi High resolution fluorescence microscopy evidence on the transport of immunoglobulins. Differences between mammalian IgG, F(ab')2 and avian IgY
    Carlos Sevcik
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas, Apartado 20632, Caracas 1020A, Bolivarian Republic of Venezuela
    Toxicon 63:7-18. 2013
  4. doi Horse IgG- and ostrich IgY-F(ab')₂ groups have different affinities for mice erythrocytes and lymphocytes. Implications for avian immunoglobulin therapeutic usefulness
    Carlos Sevcik
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas IVIC, Apartado 20632, Caracas 1020A, Venezuela
    Toxicon 60:1215-21. 2012
  5. doi Scorpion toxins modify phytopathogenic fungus physiology. A possible source of new fungicides
    Galax Joya
    Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Científicas IVIC, Caracas, Venezuela
    J Agric Food Chem 59:6327-37. 2011
  6. ncbi Effect of leukocyte inhibitors benzydamine and cyclophosphamide, on lung injury caused by Tityus discrepans scorpion venom
    Gina D'suze
    Laboratory on Cellular Neuropharmacology, IVIC CBB, Apdo 20632, Caracas 1020 A, Venezuela
    Toxicon 50:1116-25. 2007
  7. doi Antibacterial activity of six novel peptides from Tityus discrepans scorpion venom. A fluorescent probe study of microbial membrane Na+ permeability changes
    Patricia Diaz
    Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Toxicon 54:802-17. 2009
  8. ncbi Molecular cloning and nucleotide sequence analysis of genes from a cDNA library of the scorpion Tityus discrepans
    Gina D'suze
    Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Biochimie 91:1010-9. 2009
  9. ncbi On the presence of antibodies against bovine, equine and poultry immunoglobulins in human IgG preparations, and its implications on antivenom production
    Carlos Sevcik
    Laboratory of Cellular Neuropharmacology, Centro de Biofísica y Bioquímica Instituto Venezolano de Investigaciones Científicas, Apartado 21827, Caracas 1020A, Venezuela
    Toxicon 51:10-6. 2008
  10. doi Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line
    Gina D'suze
    Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas IVIC Caracas, Venezuela
    Toxicon 56:1497-505. 2010

Collaborators

Detail Information

Publications23

  1. ncbi Identification of Enterobacter bacteria as saxitoxin producers in cattle's rumen and surface water from Venezuelan Savannahs
    C Sevcik
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas, Apartado 21827, Caracas1020A, Venezuela
    Toxicon 42:359-66. 2003
    ..asburiae and E. cloacae) and a strain of Klebsiella pneumoniae, were identified using standard biochemical criteria as well as gas chromatography of bacterial lipids. All these bacteria produced STX in aerobic cultures...
  2. ncbi Initial volume of a drug before it reaches the volume of distribution: pharmacokinetics of F(ab')2 antivenoms and other drugs
    Carlos Sevcik
    Laboratory of Cellular Neuropharmacology, Apartado 21827, Caracas 1020A, Venezuela
    Toxicon 50:653-65. 2007
    ..Data from the literature showed that the kinetic considerations for V(sl)(t) also apply to clevidipine, digoxin, digitoxin, lidocaine and thiopentone...
  3. doi High resolution fluorescence microscopy evidence on the transport of immunoglobulins. Differences between mammalian IgG, F(ab')2 and avian IgY
    Carlos Sevcik
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas, Apartado 20632, Caracas 1020A, Bolivarian Republic of Venezuela
    Toxicon 63:7-18. 2013
    ..No IgY was seen in bronchial and alveolar endothelium, in bile ducts or in hepatocytes...
  4. doi Horse IgG- and ostrich IgY-F(ab')₂ groups have different affinities for mice erythrocytes and lymphocytes. Implications for avian immunoglobulin therapeutic usefulness
    Carlos Sevcik
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas IVIC, Apartado 20632, Caracas 1020A, Venezuela
    Toxicon 60:1215-21. 2012
    ..Our results suggest that IgY may be a stronger activator of adaptive immunity than horse IgG in mammals. Adaptive immunity against IgY is detrimental to its IV therapeutic use in humans and other mammals...
  5. doi Scorpion toxins modify phytopathogenic fungus physiology. A possible source of new fungicides
    Galax Joya
    Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Científicas IVIC, Caracas, Venezuela
    J Agric Food Chem 59:6327-37. 2011
    ..However, the antifungal effects at short times are indicative of a direct esterase inhibition, which, with the increased membrane leakiness to Na(+), makes the fungus inviable...
  6. ncbi Effect of leukocyte inhibitors benzydamine and cyclophosphamide, on lung injury caused by Tityus discrepans scorpion venom
    Gina D'suze
    Laboratory on Cellular Neuropharmacology, IVIC CBB, Apdo 20632, Caracas 1020 A, Venezuela
    Toxicon 50:1116-25. 2007
    ..Blocking pro-inflammatory leukocyte metabolic pathways with BZ diminished FF, suggesting that neutrophil activation, inflammation and coagulation are correlated in the genesis of scorpionism acute lung injury...
  7. doi Antibacterial activity of six novel peptides from Tityus discrepans scorpion venom. A fluorescent probe study of microbial membrane Na+ permeability changes
    Patricia Diaz
    Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Toxicon 54:802-17. 2009
    ..We found no evidence of changes of K(+) or Ca(2+) concentrations neither inside nor outside the bacteria in experiments using the fluorescent dyes Fluo 4AM (10 microM) and PBFI (20 microM)...
  8. ncbi Molecular cloning and nucleotide sequence analysis of genes from a cDNA library of the scorpion Tityus discrepans
    Gina D'suze
    Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Biochimie 91:1010-9. 2009
    ..The N-terminal amino acid sequence of several peptides is reported here for the first time. A phylogenetic tree analysis is also reported, as well as three three-dimensional models of representative toxins...
  9. ncbi On the presence of antibodies against bovine, equine and poultry immunoglobulins in human IgG preparations, and its implications on antivenom production
    Carlos Sevcik
    Laboratory of Cellular Neuropharmacology, Centro de Biofísica y Bioquímica Instituto Venezolano de Investigaciones Científicas, Apartado 21827, Caracas 1020A, Venezuela
    Toxicon 51:10-6. 2008
    ..This suggests that when IgY immunotherapeutics are used in populations with an important dietary component of poultry meat and eggs, there is a risk of producing untoward reactions and less efficient antivenoms...
  10. doi Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line
    Gina D'suze
    Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas IVIC Caracas, Venezuela
    Toxicon 56:1497-505. 2010
    ..3 and 4.1% of SKBR3 apoptosis, respectively, in 5 h of exposure; the effect was larger with more prolonged exposures. Inmunohistochemistry showed that neopladines bind to SKBR3 cell surface inducing FasL and BcL-2 expression...
  11. ncbi Tityus discrepans venom produces a respiratory distress syndrome in rabbits through an indirect mechanism
    G D'suze
    Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela
    Toxicon 37:173-80. 1999
    ..We thus propose that the use of the term pulmonary oedema in scorpionism should abandoned in favor of scorpion venom respiratory distress syndrome...
  12. ncbi Antigenic cross-reactivity between sixteen venoms from scorpions belonging to six genera
    G D'suze
    Instituto Venezolano de Investigaciones Cientificas IVIC, Lab de Neurofarmacología Celular, Caracas, Venezuela
    Clin Toxicol (Phila) 45:158-63. 2007
    ..Venoms from species from other genera such as Centruroides, Broteas, Diplocentrus, Chactas, and Rhopalurus did not cross-react with Td-antibodies...
  13. ncbi Relationship between plasmatic levels of various cytokines, tumour necrosis factor, enzymes, glucose and venom concentration following Tityus scorpion sting
    G D'suze
    Lab de Neurofarmacología Celular, Biochemistry and Biophysics Center, Instituto Venezolano de Investigaciones Cientificas IVIC, Apdo 21827, Caracas 1020 A, Venezuela
    Toxicon 41:367-75. 2003
    ..Interleukin 6 (IL6) increased significantly in patients with MS symptoms. IL6 values increased with hyperamylasemia, envenoming severity and time hyperamylasemia...
  14. ncbi Ardiscretin a novel arthropod-selective toxin from Tityus discrepans scorpion venom
    G D'suze
    Laboratory of Cellular Neuropharmacology, Biochemistry and Biophysics Center, Inst venezolano de Inves Cient, Apartado 21827, Caracas 1020 A, Venezuela
    Toxicon 43:263-72. 2004
    ....
  15. ncbi Histopathological changes and inflammatory response induced by Tityus discrepans scorpion venom in rams
    G D'suze
    Laboratory of Cellular Neuropharmacology, IVIC CBB, Apdo 21827, Caracas 1020A, Venezuela
    Toxicon 44:851-60. 2004
    ..Our results suggest that not all remote targets are directly affected by the venom but that, as proposed earlier, are modified by inflammation by products produced elsewhere...
  16. ncbi Modelling Tityus scorpion venom and antivenom pharmacokinetics. Evidence of active immunoglobulin G's F(ab')2 extrusion mechanism from blood to tissues
    C Sevcik
    Laboratory of Cellular Neuropharmacology, Instituto Venezolano de Investigaciones Cientificas IVIC, Apartado 21827, Caracas 1020A, Venezuela
    Toxicon 44:731-41. 2004
    ..Only approximately 5% of the venom is eliminated in 10h; approximately 80% of the venom is in the tissues after 2h and remains there for >10h...
  17. doi Scorpion venom complexity fractal analysis. Its relevance for comparing venoms
    Gina D'suze
    Laboratory on Cellular Neuropharmacology, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas, IVIC CBB, Apartado 20632, Caracas 1020A, Venezuela
    J Theor Biol 267:405-16. 2010
    ....
  18. ncbi [Effect of Tityus discrepans scorpion venom on global coagulation test. Preliminary studies]
    Josmary Brazón
    Laboratorio de Neurofarmacología Celular, Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas IVIC, Apartado 21827, Caracas 1020 A, Venezuela
    Invest Clin 49:49-58. 2008
    ..Td venom contains procoagulant components, able to shorten PTT. In addition, the venom contains anticoagulant components which prolong PT and PTT...
  19. ncbi Discrepin, a new peptide of the sub-family alpha-ktx15, isolated from the scorpion Tityus discrepans irreversibly blocks K+ -channels (IA currents) of cerebellum granular cells
    Gina D'suze
    Laboratory of Cellular Neuropharmacology, Biochemistry and Biophysics Center, Instituto Venezolano de Investigaciones Cientificas, Apartado 21827, Caracas 1020 A, Venezuela
    Arch Biochem Biophys 430:256-63. 2004
    ..The K+ -currents are inhibited in an apparently irreversible manner, whose 50% inhibitory effect is reached with a 190 nM toxin concentration. The systematic nomenclature proposed for this toxin is alpha-KTx15.6...
  20. ncbi Macrophage alteration induced by inflammatory toxins isolated from Tityus discrepans scorpion venom. The role of Na(+)/Ca(2+) exchangers
    Vanesa Ramírez-Bello
    Laboratory of Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas IVIC, Apdo 20632, Caracas 1020 A, Venezuela
    Toxicon 82:61-75. 2014
    ..A rise of [Ca(2+)]i activates the synthesis and secretion of inflammatory molecules like TNF-α, which in turn, increases the gene transcription for inducible nitric oxide synthase, resulting on NO production. ..
  21. ncbi Proteomic analysis of Tityus discrepans scorpion venom and amino acid sequence of novel toxins
    Cesar V F Batista
    Department of Molecular Medicine and Bioprocesses, Biotechnology Institute, UNAM, Cuernavaca, Mexico
    Proteomics 6:3718-27. 2006
    ..Additionally, peptides similar to defensins were also identified...
  22. ncbi The indirect effect of Tityus discrepans on rabbit pulmonary vasculature
    Eva Novoa
    Unit Of Respiration Physiology, Instituto de Medicina Experimental, Facultad de Medicina, Escuela Luis Razetti, Universidad Central de Venezuela, Apartado 50587, Caracas 1050, Venezuela
    Respir Physiol Neurobiol 134:33-41. 2003
    ..These experiments also make it unlikely that vascular endothelium is the source of the humoral factors contained in inflammatory serum...
  23. ncbi Pharmacokinetics of whole IgG equine antivenom: comparison between normal and envenomed rabbits
    Lil Quesada
    Facultad de Microbiologia, Instituto Clodomiro Picado, Universidad de Costa Rica, San Jose, Costa Rica
    Toxicon 48:255-63. 2006
    ..However, this corresponds to a low percentage of the administered antivenom and, therefore, this increased local extravasation does not have a significant impact in the overall antivenom pharmacokinetics...