Oxidative damage to mouse lens in culture. Protective effect of pyruvateS D Varma
Department of Biochemistry, University of Maryland School of Medicine, 10 South Pine Street, MSTF 500 A, Baltimore, 21201, USA
Biochim Biophys Acta 1621:246-52. 2003
....
- Effect of alpha-ketoglutarate against selenite cataract formation
S D Varma
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21202, USA
Exp Eye Res 79:913-8. 2004
..Whether the effectiveness is related only to its action as a ROS scavenger or it could be contributed also by some metabolic effects independent of ROS remains to be determined...
- Oxidative damage to lens in culture: reversibility by pyruvate and ethyl pyruvate
S D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Ophthalmologica 220:52-7. 2006
..This was apparent by better maintenance of the active rubidium transport activity in these lenses compared to controls without pyruvate treatment. Glutathione levels were also higher in the pyruvate group...
- Lens thiol depletion by peroxynitrite. Protective effect of pyruvate
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, MSTF, 5 00A, 10 South Pine Street, Baltimore, MD 21201, USA
Mol Cell Biochem 298:199-204. 2007
..The findings therefore appear potentially useful to protect against nitrite induced damage to the lens and other tissues known to occur with aging and certain diseases such as diabetes...
- Susceptibility of the ocular lens to nitric oxide: implications in cataractogenesis
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Ocul Pharmacol Ther 23:188-95. 2007
..The results, therefore, were highly suggestive of a contribution of the oxides of nitrogen in cataract formation associated with diabetes and other aging diseases...
- Attenuation and delay of diabetic cataracts by antioxidants: effectiveness of pyruvate after onset of cataract
S D Varma
Department of Ophthalmology and Visual Sciences, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
Ophthalmologica 219:309-15. 2005
..The latter usually comes much later than the onset of visual aberrations. Prophylaxis is not an absolute requirement...
Kynurenine-induced photo oxidative damage to lens in vitro: protective effect of caffeineShambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Cell Biochem 340:49-54. 2010
..Further studies on the pharmacological significance of the findings are hence in progress...
Prevention of oxidative damage to lens by caffeineShambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
J Ocul Pharmacol Ther 26:73-7. 2010
..The primary objective of this study was to examine the possibility of inhibiting oxidative damage to the lens in vitro by caffeine...
- UV-B-induced damage to the lens in vitro: prevention by caffeine
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Ocul Pharmacol Ther 24:439-44. 2008
..The addition of caffeine to the medium prevented such deleterious effects. That caffeine and perhaps other xanthinoids have a protective effect against cataract formation induced by UV has hence been demonstrated for the first time...
- Inhibition of selenite-induced cataract by caffeine
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine Baltimore, 21201, USA
Acta Ophthalmol 88:e245-9. 2010
..The objective of the investigation was to study possible inhibition of oxidative stress and cataract formation by caffeine in vivo...
- Induction of ultraviolet cataracts in vitro: prevention by pyruvate
Kavita R Hegde
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Ocul Pharmacol Ther 23:492-502. 2007
..Hence, further studies on the possible protective effects of such alpha-keto acids against UV damage are in progress...
- Role of ultraviolet irradiation and oxidative stress in cataract formation-medical prevention by nutritional antioxidants and metabolic agonists
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Eye Contact Lens 37:233-45. 2011
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- Combination of glycemic and oxidative stress in lens: implications in augmentation of cataract formation in diabetes
K R Hegde
Department of Biochemistry, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Free Radic Res 39:513-7. 2005
..Similarly, the galactosemic lenses were also more susceptible to menadione dependent decrease in ATP and GSH...
- Effect of high sugar levels on miRNA expression. Studies with galactosemic mice lenses
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland, Baltimore, MD 21201, USA
Mol Vis 18:1609-18. 2012
..In addition, it was intended to investigate if such expression could be antagonized by reactive oxygen species (ROS) scavengers...
- Prevention of oxidative stress to the retina by pyruvate. A preliminary report
Kavita R Hegde
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Ophthalmologica 222:194-8. 2008
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- Inhibition of glycolysis in the retina by oxidative stress: prevention by pyruvate
K R Hegde
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Cell Biochem 343:101-5. 2010
..This effect was also reflected by the elevation of NAD/NADH ratio, a major pacemaker of glycolysis...
- Effectiveness of topical caffeine in cataract prevention: studies with galactose cataract
Shambhu D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Vis 16:2626-33. 2010
..It was hypothesized that caffeine will do so by acting as scavenger of reactive oxygen species known to be generated under hyperglycemic conditions...
- Protective effect of ascorbate against oxidative stress in the mouse lens
K R Hegde
Departments of Biochemistry and Ophthalmology, University of Maryland School of Medicine, 10 South Pine Street, MSTF 500-A, Baltimore, MD 21201, USA
Biochim Biophys Acta 1670:12-8. 2004
..The findings therefore appear to be significant from the point of view of using this nutrient for delaying the onset of cataract development in human beings, therapeutically as well as nutritionally...
- Oxidative stress in lens in vivo: inhibitory effect of caffeine. A preliminary report
S D Varma
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Vis 16:501-5. 2010
..Experiments have been conducted to study the hypothesis that caffeine would inhibit reactive oxygen species induced oxidative stress in the lens in vivo, with implications of attenuating or preventing cataract formation...
- Prevention of cataract by pyruvate in experimentally diabetic mice
K R Hegde
Departments of Biochemistry and Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Cell Biochem 269:115-20. 2005
..Inhibition of cataract formation by pyruvate in an animal model with low lens AR, similar to that in humans, has been shown for the first time...
- Morphogenetic and apoptotic changes in diabetic cataract: prevention by pyruvate
K R Hegde
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Cell Biochem 262:233-7. 2004
..These changes were prevented by simultaneous administration of pyruvate to the diabetic animals. The preventive effect of this agent is attributable to its property of scavenging oxy-radicals generated by high levels of the sugars...
- Ophthalmoscopic and morphogenetic changes in rat lens induced by galactose: attenuation by pyruvate
S Kalakonda
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 212019, USA
Diabetes Obes Metab 6:216-22. 2004
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- Cataracts in experimentally diabetic mouse: morphological and apoptotic changes
K R Hegde
Departments of Ophthalmology and Biochemistry, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Diabetes Obes Metab 7:200-4. 2005
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- Establishment of the mouse as a model animal for the study of diabetic cataracts
K R Hegde
University of Maryland School of Medicine, Baltimore, MD, USA
Ophthalmic Res 35:12-8. 2003
..These studies therefore suggest that further studies with mice may throw additional light on the contribution of diabetes in the pathogenesis of cataracts in low lens aldose reductase models...
- Prevention of selenite cataract by vitamin C
P S Devamanoharan
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore 21201
Exp Eye Res 52:563-8. 1991
..The observations indicate that selenite cataract is due to an oxidative stress to the lens. In addition, the findings are in conformity with our view that ascorbate functions as an anticataractogenic substance...
- Intraocular penetration of pyruvate following its topical administration in mice
K R Hegde
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Mol Cell Biochem 338:87-90. 2010
..The results are therefore considered pharmacological significant from the point of view of its potential use for topical treatment of cataracts induced by oxidative stress and diabetes...
- Establishment of mouse as an animal model for study of diabetic cataracts: biochemical studies
K R Hegde
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD, USA
Diabetes Obes Metab 5:113-9. 2003
..Sorbitol accumulation in low aldose reductase situations, being minor, could, however, act synergistically with other factors...
- Electron impact mass spectroscopic studies on mouse retinal fatty acids: effect of diabetes
K R Hegde
Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Ophthalmic Res 42:9-14. 2009
..Additionally, in order to ascertain if there is any accumulation of lipids in the diabetic retina as occurs in many diabetic tissues, its total fatty acid content was also determined...
