Shambhu D Varma

Summary

Publications

  1. ncbi request reprint Oxidative damage to mouse lens in culture. Protective effect of pyruvate
    S D Varma
    Department of Biochemistry, University of Maryland School of Medicine, 10 South Pine Street, MSTF 500 A, Baltimore, 21201, USA
    Biochim Biophys Acta 1621:246-52. 2003
  2. ncbi request reprint Effect of alpha-ketoglutarate against selenite cataract formation
    S D Varma
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21202, USA
    Exp Eye Res 79:913-8. 2004
  3. ncbi request reprint Oxidative damage to lens in culture: reversibility by pyruvate and ethyl pyruvate
    S D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Ophthalmologica 220:52-7. 2006
  4. ncbi request reprint Lens thiol depletion by peroxynitrite. Protective effect of pyruvate
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, MSTF, 5 00A, 10 South Pine Street, Baltimore, MD 21201, USA
    Mol Cell Biochem 298:199-204. 2007
  5. ncbi request reprint Susceptibility of the ocular lens to nitric oxide: implications in cataractogenesis
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Ocul Pharmacol Ther 23:188-95. 2007
  6. ncbi request reprint Attenuation and delay of diabetic cataracts by antioxidants: effectiveness of pyruvate after onset of cataract
    S D Varma
    Department of Ophthalmology and Visual Sciences, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Ophthalmologica 219:309-15. 2005
  7. doi request reprint Kynurenine-induced photo oxidative damage to lens in vitro: protective effect of caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Cell Biochem 340:49-54. 2010
  8. pmc Prevention of oxidative damage to lens by caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Ocul Pharmacol Ther 26:73-7. 2010
  9. pmc UV-B-induced damage to the lens in vitro: prevention by caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Ocul Pharmacol Ther 24:439-44. 2008
  10. pmc Inhibition of selenite-induced cataract by caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine Baltimore, 21201, USA
    Acta Ophthalmol 88:e245-9. 2010

Collaborators

  • Stephen Morris
  • K R Hegde
  • Kavita R Hegde
  • S Kovtun
  • S Kalakonda
  • M G Henein
  • Svitlana Kovtun
  • P S Devamanoharan
  • R D Richards
  • S Ramachandran
  • M Henein

Detail Information

Publications29

  1. ncbi request reprint Oxidative damage to mouse lens in culture. Protective effect of pyruvate
    S D Varma
    Department of Biochemistry, University of Maryland School of Medicine, 10 South Pine Street, MSTF 500 A, Baltimore, 21201, USA
    Biochim Biophys Acta 1621:246-52. 2003
    ....
  2. ncbi request reprint Effect of alpha-ketoglutarate against selenite cataract formation
    S D Varma
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21202, USA
    Exp Eye Res 79:913-8. 2004
    ..Whether the effectiveness is related only to its action as a ROS scavenger or it could be contributed also by some metabolic effects independent of ROS remains to be determined...
  3. ncbi request reprint Oxidative damage to lens in culture: reversibility by pyruvate and ethyl pyruvate
    S D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Ophthalmologica 220:52-7. 2006
    ..This was apparent by better maintenance of the active rubidium transport activity in these lenses compared to controls without pyruvate treatment. Glutathione levels were also higher in the pyruvate group...
  4. ncbi request reprint Lens thiol depletion by peroxynitrite. Protective effect of pyruvate
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, MSTF, 5 00A, 10 South Pine Street, Baltimore, MD 21201, USA
    Mol Cell Biochem 298:199-204. 2007
    ..The findings therefore appear potentially useful to protect against nitrite induced damage to the lens and other tissues known to occur with aging and certain diseases such as diabetes...
  5. ncbi request reprint Susceptibility of the ocular lens to nitric oxide: implications in cataractogenesis
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Ocul Pharmacol Ther 23:188-95. 2007
    ..The results, therefore, were highly suggestive of a contribution of the oxides of nitrogen in cataract formation associated with diabetes and other aging diseases...
  6. ncbi request reprint Attenuation and delay of diabetic cataracts by antioxidants: effectiveness of pyruvate after onset of cataract
    S D Varma
    Department of Ophthalmology and Visual Sciences, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Ophthalmologica 219:309-15. 2005
    ..The latter usually comes much later than the onset of visual aberrations. Prophylaxis is not an absolute requirement...
  7. doi request reprint Kynurenine-induced photo oxidative damage to lens in vitro: protective effect of caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Cell Biochem 340:49-54. 2010
    ..Further studies on the pharmacological significance of the findings are hence in progress...
  8. pmc Prevention of oxidative damage to lens by caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Ocul Pharmacol Ther 26:73-7. 2010
    ..The primary objective of this study was to examine the possibility of inhibiting oxidative damage to the lens in vitro by caffeine...
  9. pmc UV-B-induced damage to the lens in vitro: prevention by caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Ocul Pharmacol Ther 24:439-44. 2008
    ..The addition of caffeine to the medium prevented such deleterious effects. That caffeine and perhaps other xanthinoids have a protective effect against cataract formation induced by UV has hence been demonstrated for the first time...
  10. pmc Inhibition of selenite-induced cataract by caffeine
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine Baltimore, 21201, USA
    Acta Ophthalmol 88:e245-9. 2010
    ..The objective of the investigation was to study possible inhibition of oxidative stress and cataract formation by caffeine in vivo...
  11. ncbi request reprint Induction of ultraviolet cataracts in vitro: prevention by pyruvate
    Kavita R Hegde
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Ocul Pharmacol Ther 23:492-502. 2007
    ..Hence, further studies on the possible protective effects of such alpha-keto acids against UV damage are in progress...
  12. pmc Role of ultraviolet irradiation and oxidative stress in cataract formation-medical prevention by nutritional antioxidants and metabolic agonists
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Eye Contact Lens 37:233-45. 2011
    ....
  13. ncbi request reprint Combination of glycemic and oxidative stress in lens: implications in augmentation of cataract formation in diabetes
    K R Hegde
    Department of Biochemistry, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Free Radic Res 39:513-7. 2005
    ..Similarly, the galactosemic lenses were also more susceptible to menadione dependent decrease in ATP and GSH...
  14. pmc Protective effect of caffeine against high sugar-induced transcription of microRNAs and consequent gene silencing: a study using lenses of galactosemic mice
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Vis 19:493-500. 2013
    ..The objective of this study was to investigate if this protective effect is reflected in the attenuation of the transcription of microRNAs (miRNAs) known to induce apoptosis and cell death by gene silencing...
  15. pmc Effect of high sugar levels on miRNA expression. Studies with galactosemic mice lenses
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland, Baltimore, MD 21201, USA
    Mol Vis 18:1609-18. 2012
    ..In addition, it was intended to investigate if such expression could be antagonized by reactive oxygen species (ROS) scavengers...
  16. doi request reprint Prevention of oxidative stress to the retina by pyruvate. A preliminary report
    Kavita R Hegde
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Ophthalmologica 222:194-8. 2008
    ....
  17. pmc Inhibition of glycolysis in the retina by oxidative stress: prevention by pyruvate
    K R Hegde
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Cell Biochem 343:101-5. 2010
    ..This effect was also reflected by the elevation of NAD/NADH ratio, a major pacemaker of glycolysis...
  18. pmc Effectiveness of topical caffeine in cataract prevention: studies with galactose cataract
    Shambhu D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Vis 16:2626-33. 2010
    ..It was hypothesized that caffeine will do so by acting as scavenger of reactive oxygen species known to be generated under hyperglycemic conditions...
  19. ncbi request reprint Protective effect of ascorbate against oxidative stress in the mouse lens
    K R Hegde
    Departments of Biochemistry and Ophthalmology, University of Maryland School of Medicine, 10 South Pine Street, MSTF 500 A, Baltimore, MD 21201, USA
    Biochim Biophys Acta 1670:12-8. 2004
    ..The findings therefore appear to be significant from the point of view of using this nutrient for delaying the onset of cataract development in human beings, therapeutically as well as nutritionally...
  20. pmc Oxidative stress in lens in vivo: inhibitory effect of caffeine. A preliminary report
    S D Varma
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Vis 16:501-5. 2010
    ..Experiments have been conducted to study the hypothesis that caffeine would inhibit reactive oxygen species induced oxidative stress in the lens in vivo, with implications of attenuating or preventing cataract formation...
  21. ncbi request reprint Prevention of cataract by pyruvate in experimentally diabetic mice
    K R Hegde
    Departments of Biochemistry and Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Cell Biochem 269:115-20. 2005
    ..Inhibition of cataract formation by pyruvate in an animal model with low lens AR, similar to that in humans, has been shown for the first time...
  22. ncbi request reprint Morphogenetic and apoptotic changes in diabetic cataract: prevention by pyruvate
    K R Hegde
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Cell Biochem 262:233-7. 2004
    ..These changes were prevented by simultaneous administration of pyruvate to the diabetic animals. The preventive effect of this agent is attributable to its property of scavenging oxy-radicals generated by high levels of the sugars...
  23. ncbi request reprint Ophthalmoscopic and morphogenetic changes in rat lens induced by galactose: attenuation by pyruvate
    S Kalakonda
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD 212019, USA
    Diabetes Obes Metab 6:216-22. 2004
    ..Investigations have been conducted on the potential of pyruvate, a normal tissue metabolite, in the prevention of cataract formation...
  24. ncbi request reprint Establishment of the mouse as a model animal for the study of diabetic cataracts
    K R Hegde
    University of Maryland School of Medicine, Baltimore, MD, USA
    Ophthalmic Res 35:12-8. 2003
    ..These studies therefore suggest that further studies with mice may throw additional light on the contribution of diabetes in the pathogenesis of cataracts in low lens aldose reductase models...
  25. ncbi request reprint Cataracts in experimentally diabetic mouse: morphological and apoptotic changes
    K R Hegde
    Departments of Ophthalmology and Biochemistry, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Diabetes Obes Metab 7:200-4. 2005
    ..Previous studies were done primarily at biochemical level...
  26. ncbi request reprint Prevention of selenite cataract by vitamin C
    P S Devamanoharan
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore 21201
    Exp Eye Res 52:563-8. 1991
    ..The observations indicate that selenite cataract is due to an oxidative stress to the lens. In addition, the findings are in conformity with our view that ascorbate functions as an anticataractogenic substance...
  27. doi request reprint Intraocular penetration of pyruvate following its topical administration in mice
    K R Hegde
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Cell Biochem 338:87-90. 2010
    ..The results are therefore considered pharmacological significant from the point of view of its potential use for topical treatment of cataracts induced by oxidative stress and diabetes...
  28. ncbi request reprint Establishment of mouse as an animal model for study of diabetic cataracts: biochemical studies
    K R Hegde
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, MD, USA
    Diabetes Obes Metab 5:113-9. 2003
    ..The primary aim of this study was to understand the pathogenesis of diabetic cataracts at biochemical level in an animal model where lens aldose reductase (AR) activity is low, similar to that in the human lens...
  29. pmc Electron impact mass spectroscopic studies on mouse retinal fatty acids: effect of diabetes
    K R Hegde
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Ophthalmic Res 42:9-14. 2009
    ..Additionally, in order to ascertain if there is any accumulation of lipids in the diabetic retina as occurs in many diabetic tissues, its total fatty acid content was also determined...