Ning Wei

Summary

Affiliation: Yale University
Country: USA

Publications

  1. ncbi request reprint The COP9 signalosome
    Ning Wei
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Annu Rev Cell Dev Biol 19:261-86. 2003
  2. pmc The COP9 signalosome interacts with SCF UFO and participates in Arabidopsis flower development
    Xiping Wang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Plant Cell 15:1071-82. 2003
  3. pmc Characterization of the last subunit of the Arabidopsis COP9 signalosome: implications for the overall structure and origin of the complex
    Giovanna Serino
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Plant Cell 15:719-31. 2003
  4. pmc Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome
    Suchithra Menon
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA
    BMC Biochem 9:1. 2008
  5. pmc Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes
    Elah Pick
    Department of Molecualr, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Mol Cell Biol 27:4708-19. 2007
  6. pmc The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses
    Suhua Feng
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Plant Cell 15:1083-94. 2003
  7. ncbi request reprint Purification of the COP9 signalosome from porcine spleen, human cell lines, and Arabidopsis thaliana plants
    Suchithra Menon
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8104, USA
    Methods Enzymol 398:468-81. 2005
  8. pmc Disruption of the COP9 signalosome Csn2 subunit in mice causes deficient cell proliferation, accumulation of p53 and cyclin E, and early embryonic death
    Karin Lykke-Andersen
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Mol Cell Biol 23:6790-7. 2003
  9. ncbi request reprint CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex
    Jianyu Zheng
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, Yale University, New Haven, CT 06520, USA
    Mol Cell 10:1519-26. 2002
  10. doi request reprint The COP9 signalosome: more than a protease
    Ning Wei
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Trends Biochem Sci 33:592-600. 2008

Research Grants

Collaborators

Detail Information

Publications27

  1. ncbi request reprint The COP9 signalosome
    Ning Wei
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Annu Rev Cell Dev Biol 19:261-86. 2003
    ..The involvement of CSN in a number of cellular and developmental processes has been attributed to its control over ubiquitin-proteasome-mediated protein degradation...
  2. pmc The COP9 signalosome interacts with SCF UFO and participates in Arabidopsis flower development
    Xiping Wang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Plant Cell 15:1071-82. 2003
    ..We conclude that CSN is an essential regulator of Arabidopsis flower development and suggest that CSN regulates Arabidopsis flower development in part by modulating SCF(UFO)-mediated AP3 activation...
  3. pmc Characterization of the last subunit of the Arabidopsis COP9 signalosome: implications for the overall structure and origin of the complex
    Giovanna Serino
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Plant Cell 15:719-31. 2003
    ..Comparative analyses of the subunit interaction of CSN revealed a set of conserved subunit contacts and resulted in a model of CSN subunit topology, some aspects of which were substantiated by in vivo cross-link tests...
  4. pmc Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome
    Suchithra Menon
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA
    BMC Biochem 9:1. 2008
    ..The level of Nedd8 modification is critically dependent on the COP9 signalosome (CSN), an eight-subunit protein complex containing Nedd8 isopeptidase activity...
  5. pmc Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes
    Elah Pick
    Department of Molecualr, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Mol Cell Biol 27:4708-19. 2007
    ..Overexpression of DET1 inhibits UV-induced CDT1 degradation in cultured cells. These findings demonstrate that the conserved DET1 complex modulates Cul4A functions by a novel mechanism...
  6. pmc The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses
    Suhua Feng
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Plant Cell 15:1083-94. 2003
    ..Furthermore, we showed that both COI1 and CSN are essential for modulating the expression of genes in most cellular pathways responsive to JA. Thus, CSN and SCF(COI1) work together to control genome expression and promote JA responses...
  7. ncbi request reprint Purification of the COP9 signalosome from porcine spleen, human cell lines, and Arabidopsis thaliana plants
    Suchithra Menon
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8104, USA
    Methods Enzymol 398:468-81. 2005
    ..It also describes in vitro deneddylation assays using the HeLa cell extract or the Arabidopsis cell extract, which we have used to test and compare the activity of purified CSN complexes...
  8. pmc Disruption of the COP9 signalosome Csn2 subunit in mice causes deficient cell proliferation, accumulation of p53 and cyclin E, and early embryonic death
    Karin Lykke-Andersen
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Mol Cell Biol 23:6790-7. 2003
    ..Furthermore, Csn2(-/-) embryos contained elevated levels of p53 and p21, which may contribute to premature cell cycle arrest of the mutant...
  9. ncbi request reprint CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex
    Jianyu Zheng
    Department of Genetics, Yale University School of Medicine, 333 Cedar Street, Yale University, New Haven, CT 06520, USA
    Mol Cell 10:1519-26. 2002
    ..Our data suggest that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F box proteins into the SCF complex, causing their destabilization...
  10. doi request reprint The COP9 signalosome: more than a protease
    Ning Wei
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Trends Biochem Sci 33:592-600. 2008
    ..The independent functions of CSN5 (also known as JAB1) add to the complexity of the CSN machinery. Here, we provide an updated view of the structure, functions and regulation of this protein complex...
  11. ncbi request reprint Gene structure and embryonic expression of mouse COP9 signalosome subunit 8 (Csn8)
    Karin Lykke-Andersen
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208104, New Haven, CT 06520 8104, USA
    Gene 321:65-72. 2003
    ..Immunostaining studies revealed that Csn8 is predominantly present in the inner cell mass (ICM) of E3.5 blastocyst and is widely expressed in E9.5-day embryos...
  12. ncbi request reprint Affinity purification reveals the association of WD40 protein constitutive photomorphogenic 1 with the hetero-oligomeric TCP-1 chaperonin complex in mammalian cells
    Chunling Yi
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208104, 165 Prospect Street, OML 352A, New Haven, CT 06520 8104, USA
    Int J Biochem Cell Biol 38:1076-83. 2006
    ..The association of WD40 protein COP1 with TRiC/Hsp70 in mammalian cells suggests that facilitating the folding of WD40 proteins may be a conserved function for TRiC/Hsp70 from yeast to mammals...
  13. pmc An initial biochemical and cell biological characterization of the mammalian homologue of a central plant developmental switch, COP1
    Chunling Yi
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8104, USA
    BMC Cell Biol 3:30. 2002
    ..Although COP1 mammalian homologue has been previously reported, its function and distribution in animal kingdom are not known...
  14. doi request reprint CSN1 inhibits c-Jun phosphorylation and down-regulates ectopic expression of JNK1
    Tomohiko Tsuge
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, 06520, USA
    Protein Cell 2:423-32. 2011
    ..Thus, in contrast to CSN5/Jab1, which promotes AP-1 activity, CSN1 displays a negative effect on the AP-1 pathway. Finally, we discuss about the dynamic equilibrium of the CSN complexes in regulation of the AP-1 pathway...
  15. ncbi request reprint Major vault protein, in concert with constitutively photomorphogenic 1, negatively regulates c-Jun-mediated activator protein 1 transcription in mammalian cells
    Chunling Yi
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8104, USA
    Cancer Res 65:5835-40. 2005
    ..Taken together, we propose that MVP, most likely through its interaction with COP1, suppresses c-Jun-mediated AP-1 transcription under unstressed conditions, thereby preventing cells from undergoing stress response...
  16. ncbi request reprint COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence
    Suchithra Menon
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06511, USA
    Nat Immunol 8:1236-45. 2007
    ..Our data define a CSN-dependent pathway of transcriptional control that is essential for antigen-induced initiation of T cell proliferation...
  17. pmc CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome
    Xiping Wang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520
    Mol Biol Cell 13:646-55. 2002
    ..We conclude that CSN1 N-terminal domain is not required for the Rub1/Nedd8 deconjugation activity of cullins, but contributes to a significant aspect of CSN functions that are essential for plant development...
  18. pmc Phosphorylation of FAR-RED ELONGATED HYPOCOTYL1 is a key mechanism defining signaling dynamics of phytochrome A under red and far-red light in Arabidopsis
    Fang Chen
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Conecticut 06520, USA
    Plant Cell 24:1907-20. 2012
    ..Collectively, our results reveal FHY1 phosphorylation as a key mechanism for FR/R spectrum-specific responses in plants and an essential event for plant adaption to changing light conditions in nature...
  19. ncbi request reprint The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1
    Xiaoming Yang
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA
    Curr Biol 12:667-72. 2002
    ..We conclude that CSN inhibits SCF ubiquitin ligase activity in targeting p27 proteolysis and negatively regulates cell cycle at the G1 phase by promoting deneddylation of Cul1...
  20. pmc COP9 signalosome subunit Csn8 is involved in maintaining proper duration of the G1 phase
    Cheng Liu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 288:20443-52. 2013
    ..We conclude that CSN harbors both positive and negative regulators of the cell cycle and therefore is poised to influence the fate of a cell at the crossroad of cell division, differentiation, and senescence. ..
  21. doi request reprint Cullin-RING ubiquitin ligase family in plant abiotic stress pathways(F)
    Liquan Guo
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven 06520, Connecticut, USA
    J Integr Plant Biol 55:21-30. 2013
    ..By comparing and analyzing these cases, we hope to gain a better understanding on how CRL complexes work under various settings in an attempt to decipher the clues about the regulatory mechanism of CRL E3s...
  22. pmc Degradation of the bile salt export pump at endoplasmic reticulum in progressive familial intrahepatic cholestasis type II
    Lin Wang
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06510, USA
    Hepatology 48:1558-69. 2008
    ..Furthermore, we present evidence that G982R weakly associates with various components of the ER quality control system. These data together demonstrate that the PFIC II mutants except R1153C and DeltaGly are degraded by the ERAD pathway...
  23. pmc COP9 signalosome subunit 3 is essential for maintenance of cell proliferation in the mouse embryonic epiblast
    Jiong Yan
    Department of Molecular and Human Genetics, Texas Children s Hospital, Houston, Texas 77030, USA
    Mol Cell Biol 23:6798-808. 2003
    ..Therefore, Csn3 is important for maintaining the integrity of the COP9 signalosome and is crucial to maintain the survival of epiblast cells and thus the development of the postimplantation embryo in mice...
  24. ncbi request reprint DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
    Kenneth Wu
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:28882-91. 2003
    ..These results suggest a unique role for DEN1 in regulating the modification of cullins by Nedd8...
  25. ncbi request reprint Light regulated modulation of Z-box containing promoters by photoreceptors and downstream regulatory components, COP1 and HY5, in Arabidopsis
    Vandana Yadav
    National Center for Plant Genome Research, JNU Campus, New Delhi 110067, India
    Plant J 31:741-53. 2002
    ..DNA-protein interaction studies reveal the presence of a DNA-binding activity that is specific to Z-box. These results provide insights into the regulation of the Z-box LRE mediated by various light signaling components...
  26. ncbi request reprint A simple and reliable assay for detecting specific nucleotide sequences in plants using optical thin-film biosensor chips
    Su Lan Bai
    Peking Yale Joint Center for Plant Molecular Genetics and Agro Biotechnology, and The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Plant J 49:354-66. 2007
    ..This technology can be customized for any nucleotide sequence-based identification assay and widely applied in crop breeding, trait mapping, and other work requiring positive detection of specific nucleotide sequences...
  27. ncbi request reprint A draft sequence for the genome of the domesticated silkworm (Bombyx mori)
    Qingyou Xia
    Southwest Agricultural University, Chongqing Beibei, 400716, China
    Science 306:1937-40. 2004
    ..Our estimated gene count is 18,510, which exceeds the 13,379 genes reported for Drosophila melanogaster. Comparative analyses to fruitfly, mosquito, spider, and butterfly reveal both similarities and differences in gene content...

Research Grants6

  1. STRUCTURE AND FUNCTION OF THE COP9 SIGNALOSOME
    Ning Wei; Fiscal Year: 2005
    ....