Derek Toomre

Summary

Affiliation: Yale University
Country: USA

Publications

  1. doi request reprint A new wave of cellular imaging
    Derek Toomre
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    Annu Rev Cell Dev Biol 26:285-314. 2010
  2. pmc Spying on IgE receptor signaling: simply complex, or not?
    Derek Toomre
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 171:415-7. 2005
  3. pmc A phosphoinositide switch controls the maturation and signaling properties of APPL endosomes
    Roberto Zoncu
    Department of Cell Biology, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Cell 136:1110-21. 2009
  4. pmc The inositol 5-phosphatase SHIP2 regulates endocytic clathrin-coated pit dynamics
    Fubito Nakatsu
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 190:307-15. 2010
  5. pmc A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway
    Kai S Erdmann
    Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06510, USA
    Dev Cell 13:377-90. 2007
  6. ncbi request reprint Estimation of 3D geometry of microtubules using multi-angle total internal reflection fluorescence microscopy
    Qian Yang
    Department of Electrical Engineering, Yale University, New Haven, CT, USA
    Med Image Comput Comput Assist Interv 13:538-45. 2010
  7. pmc Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate
    Roberto Zoncu
    Department of Cell Biology and Howard Hughes Medical Institute, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 104:3793-8. 2007
  8. pmc Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion
    Felix Rivera-Molina
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 201:673-80. 2013
  9. pmc Splice isoform estrogen receptors as integral transmembrane proteins
    Kyung Hee Kim
    Departments of Internal Medicine Cardiovascular Medicine and Immunobiology and the Raymond and Beverly Sackler Foundation Cardiovascular Laboratory, Yale University School of Medicine, New Haven, CT 06511, USA
    Mol Biol Cell 22:4415-23. 2011
  10. pmc Repair of injured plasma membrane by rapid Ca2+-dependent endocytosis
    Vincent Idone
    Section of Microbial Pathogenesis and 2Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 180:905-14. 2008

Collaborators

Detail Information

Publications30

  1. doi request reprint A new wave of cellular imaging
    Derek Toomre
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    Annu Rev Cell Dev Biol 26:285-314. 2010
    ..We emphasize major advances, biological applications, and promising new developments...
  2. pmc Spying on IgE receptor signaling: simply complex, or not?
    Derek Toomre
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 171:415-7. 2005
    ..CC-FCS data from studies of IgE receptor signaling challenge models of large stable lipid raft signaling domains and reveal a new complexity in the dynamic (re)organization of signaling complexes...
  3. pmc A phosphoinositide switch controls the maturation and signaling properties of APPL endosomes
    Roberto Zoncu
    Department of Cell Biology, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Cell 136:1110-21. 2009
    ..These findings reveal a surprising plasticity of the early endocytic pathway. Importantly, PI3P functions as a switch to dynamically regulate maturation and signaling of APPL endosomes...
  4. pmc The inositol 5-phosphatase SHIP2 regulates endocytic clathrin-coated pit dynamics
    Fubito Nakatsu
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 190:307-15. 2010
    ..Because SHIP2 is a negative regulator of insulin signaling, our findings suggest the importance of the phosphoinositide metabolism at CCPs in the regulation of insulin signal output...
  5. pmc A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway
    Kai S Erdmann
    Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06510, USA
    Dev Cell 13:377-90. 2007
    ..Our results support a role of OCRL in the early endocytic pathway, consistent with the predominant localization of its preferred substrates, PI(4,5)P(2) and PI(3,4,5)P(3), at the cell surface...
  6. ncbi request reprint Estimation of 3D geometry of microtubules using multi-angle total internal reflection fluorescence microscopy
    Qian Yang
    Department of Electrical Engineering, Yale University, New Haven, CT, USA
    Med Image Comput Comput Assist Interv 13:538-45. 2010
    ..We apply our method to TIRF images of microtubules in PTK2 cells and compare the distribution of the microtubule curvatures with electron microscopy (EM) images...
  7. pmc Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate
    Roberto Zoncu
    Department of Cell Biology and Howard Hughes Medical Institute, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 104:3793-8. 2007
    ..These findings demonstrate the critical importance of PI(4,5)P(2) in clathrin coat dynamics and Arp2/3-dependent actin regulation...
  8. pmc Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion
    Felix Rivera-Molina
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 201:673-80. 2013
    ..Additionally, Sec8 dynamically repositioned to the site of membrane expansion, suggesting that it may respond to local cues during early cell polarization...
  9. pmc Splice isoform estrogen receptors as integral transmembrane proteins
    Kyung Hee Kim
    Departments of Internal Medicine Cardiovascular Medicine and Immunobiology and the Raymond and Beverly Sackler Foundation Cardiovascular Laboratory, Yale University School of Medicine, New Haven, CT 06511, USA
    Mol Biol Cell 22:4415-23. 2011
    ..Thus there exist pools of transmembrane steroid hormone receptors that are efficient signaling molecules and potential novel therapeutic targets...
  10. pmc Repair of injured plasma membrane by rapid Ca2+-dependent endocytosis
    Vincent Idone
    Section of Microbial Pathogenesis and 2Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 180:905-14. 2008
    ..These findings provide an important new insight into how cells protect themselves not only from mechanical injury but also from microbial toxins and pore-forming proteins produced by the immune system...
  11. pmc A network of interactions enables CCM3 and STK24 to coordinate UNC13D-driven vesicle exocytosis in neutrophils
    Yong Zhang
    Department of Pharmacology, Yale School of Medicine, New Haven, CT 06520, USA Department of Vascular Biology and Therapeutic Program, Yale School of Medicine, New Haven, CT 06520, USA
    Dev Cell 27:215-26. 2013
    ..Together, these findings reveal a function of the STK24 and CCM3 complex in the regulation of ligand-stimulated exocytosis. ..
  12. doi request reprint 3-D reconstruction of microtubules from multi-angle total internal reflection fluorescence microscopy using Bayesian framework
    Qian Yang
    Department of Electrical Engineering, Yale University, New Haven, CT 06511, USA
    IEEE Trans Image Process 20:2248-59. 2011
    ..The presented method has the potential to provide a reliable tool for 3-D reconstruction and tracking of microtubules...
  13. pmc Exocyst is involved in polarized cell migration and cerebral cortical development
    Kresimir Letinic
    Department of Neurobiology and Kavli Institute for Neuroscience, 333 Cedar Street, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 106:11342-7. 2009
    ..Thus, our study supports the notion that polarized membrane traffic regulated by the exocyst is an essential component of cell migration and that its deficit may lead to cortical abnormalities involving cortical neuronal malpositioning...
  14. ncbi request reprint Segmentation and 3D reconstruction of microtubules in total internal reflection fluorescence microscopy (TIRFM)
    Stathis Hadjidemetriou
    Departments of Diagnostic Radiology and Biomedical Engineering, New Haven, CT 06520, USA
    Med Image Comput Comput Assist Interv 8:761-9. 2005
    ..The segments are described via Hamilton-Jacobi equations. Subsequently, the algorithm performs a limited reconstruction of the microtubules in 3D. Last, we evaluate our method with phantom as well as real TIRFM images of living cells...
  15. pmc Deciphering subcellular processes in live imaging datasets via dynamic probabilistic networks
    Kresimir Letinic
    Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Bioinformatics 26:2029-36. 2010
    ..Live cell imaging reveals changes in the cellular states, but current simple approaches extract only minimal information of a static snapshot...
  16. pmc Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms
    Fang Huang
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA
    Nat Methods 10:653-8. 2013
    ..Using these in combination with a multi-emitter fitting algorithm, we demonstrate single-molecule localization super-resolution imaging at rates of up to 32 reconstructed images per second in fixed and living cells. ..
  17. pmc The neuropilin 1 cytoplasmic domain is required for VEGF-A-dependent arteriogenesis
    Anthony Lanahan
    Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA
    Dev Cell 25:156-68. 2013
    ..These results demonstrate that the NRP1 cytoplasmic domain promotes VEGFR2 trafficking in a PDZ-dependent manner to regulate arteriogenic ERK signaling and establish a role for NRP1 in VEGF-A signaling during vascular morphogenesis...
  18. pmc The tyrosine kinase activity of c-Src regulates actin dynamics and organization of podosomes in osteoclasts
    Olivier Destaing
    Department of Orthopaedics, Yale University School of Medicine, New Haven, CT 06520, USA
    Mol Biol Cell 19:394-404. 2008
    ..Thus, Src is an essential regulator of podosome structure, dynamics and organization...
  19. pmc Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages
    Rushika M Perera
    Department of Cell Biology, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 103:19332-7. 2006
    ..These results raise the possibility that dynamic phosphoinositide metabolism may occur throughout the lifetime of a CCP...
  20. doi request reprint Motion tracking of the outer tips of microtubules
    Stathis Hadjidemetriou
    Department of Biomedical Engineering and Diagnostic Radiology, Yale University, New Haven, CT 06520, USA
    Med Image Anal 12:689-702. 2008
    ..Last, we evaluate our method with phantom as well as real data. The real data show fluorescently tagged living cells imaged with epifluorescent microscopy or confocal microscopy...
  21. pmc Par3 functions in the biogenesis of the primary cilium in polarized epithelial cells
    Jeff Sfakianos
    Department of Cell Biology, Ludwig Institute for Cancer Research, New Haven, CT 06520
    J Cell Biol 179:1133-40. 2007
    ..Thus, the Par complex likely serves as an adaptor that couples the vectorial movement of at least a subset of membrane proteins to microtubule-dependent transport during ciliogenesis...
  22. pmc Phosphatidylinositol phosphate kinase type I gamma regulates dynamics of large dense-core vesicle fusion
    Liang Wei Gong
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 102:5204-9. 2005
    ..These results provide direct genetic evidence for a key role of phosphatidylinositol-4,5-bisphosphate synthesis in the regulation of large dense-core vesicle fusion dynamics...
  23. pmc Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging
    Wei Hua
    Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 172:1035-44. 2006
    ....
  24. ncbi request reprint Golgi biogenesis in Toxoplasma gondii
    Laurence Pelletier
    Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 418:548-52. 2002
    ..Our results indicate that new Golgi grow by autonomous duplication and raise the possibility that the Golgi is a paired structure that is analogous to centrioles...
  25. ncbi request reprint Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane
    Amy Chow
    Department of Cell Biology and Section of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, Connecticut 06520 8002, USA
    Nature 418:988-94. 2002
    ..We show that on stimulation, dendritic cells generate tubules from lysosomal compartments that go on to fuse directly with the plasma membrane...
  26. doi request reprint Measuring spatiotemporal dependencies in bivariate temporal random sets with applications to cell biology
    Ester Diaz
    Department of Computer Science, University of Valencia, Burjasot, Spain
    IEEE Trans Pattern Anal Mach Intell 30:1659-71. 2008
    ....
  27. ncbi request reprint Overlapping events with application to image sequences
    Guillermo Ayala
    Departamento de Estadística e Investigación Operativa, University of Valencia, Avda Vicent Andres Estelles, 46100 Burjasot, Spain
    IEEE Trans Pattern Anal Mach Intell 28:1707-12. 2006
    ..Our results show that the proposed temporal Boolean model is effective for obtaining information about dynamic processes which exhibit short-lived, but spatially and temporally overlapping events...
  28. ncbi request reprint Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola- and F-actin-rich domains
    Jaime Millan
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
    Nat Cell Biol 8:113-23. 2006
    ....
  29. ncbi request reprint Spatio-temporal analysis of constitutive exocytosis in epithelial cells
    Rafael Sebastian
    Departamento de Informatica, Universidad de Valencia, Dr Moliner, Burjassot, Spain
    IEEE/ACM Trans Comput Biol Bioinform 3:17-32. 2006
    ..Our novel approach could be translated to other studies of membrane trafficking in health and diseases such as diabetes...
  30. ncbi request reprint Selective delivery of secretory cargo in Golgi-derived carriers of nonepithelial cells
    Amin Rustom
    Department of Neurobiology, Interdisciplinary Center of Neuroscience, University of Heidelberg, INF 364, Germany
    Traffic 3:279-88. 2002
    ..These findings suggest that nonepithelial cells, like their epithelial counterparts, possess a sorting machinery capable of selective packaging of secretory cargo into distinct types of carriers...