Research Topics
| Derek ToomreSummaryAffiliation: Yale University Country: USA Publications
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Publications
A new wave of cellular imagingDerek Toomre
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
Annu Rev Cell Dev Biol 26:285-314. 2010..We emphasize major advances, biological applications, and promising new developments...
Spying on IgE receptor signaling: simply complex, or not?Derek Toomre
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
J Cell Biol 171:415-7. 2005..CC-FCS data from studies of IgE receptor signaling challenge models of large stable lipid raft signaling domains and reveal a new complexity in the dynamic (re)organization of signaling complexes...- The inositol 5-phosphatase SHIP2 regulates endocytic clathrin-coated pit dynamicsFubito Nakatsu
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
J Cell Biol 190:307-15. 2010..Because SHIP2 is a negative regulator of insulin signaling, our findings suggest the importance of the phosphoinositide metabolism at CCPs in the regulation of insulin signal output... - A phosphoinositide switch controls the maturation and signaling properties of APPL endosomesRoberto Zoncu
Department of Cell Biology, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
Cell 136:1110-21. 2009..These findings reveal a surprising plasticity of the early endocytic pathway. Importantly, PI3P functions as a switch to dynamically regulate maturation and signaling of APPL endosomes... - A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathwayKai S Erdmann
Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06510, USA
Dev Cell 13:377-90. 2007..Our results support a role of OCRL in the early endocytic pathway, consistent with the predominant localization of its preferred substrates, PI(4,5)P(2) and PI(3,4,5)P(3), at the cell surface... 
Estimation of 3D geometry of microtubules using multi-angle total internal reflection fluorescence microscopyQian Yang
Department of Electrical Engineering, Yale University, New Haven, CT, USA
Med Image Comput Comput Assist Interv 13:538-45. 2010..We apply our method to TIRF images of microtubules in PTK2 cells and compare the distribution of the microtubule curvatures with electron microscopy (EM) images...- Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphateRoberto Zoncu
Department of Cell Biology and Howard Hughes Medical Institute, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 104:3793-8. 2007..These findings demonstrate the critical importance of PI(4,5)P(2) in clathrin coat dynamics and Arp2/3-dependent actin regulation... - Splice isoform estrogen receptors as integral transmembrane proteinsKyung Hee Kim
Departments of Internal Medicine Cardiovascular Medicine and Immunobiology and the Raymond and Beverly Sackler Foundation Cardiovascular Laboratory, Yale University School of Medicine, New Haven, CT 06511, USA
Mol Biol Cell 22:4415-23. 2011..Thus there exist pools of transmembrane steroid hormone receptors that are efficient signaling molecules and potential novel therapeutic targets... - Repair of injured plasma membrane by rapid Ca2+-dependent endocytosisVincent Idone
Section of Microbial Pathogenesis and 2Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
J Cell Biol 180:905-14. 2008..These findings provide an important new insight into how cells protect themselves not only from mechanical injury but also from microbial toxins and pore-forming proteins produced by the immune system... - Exocyst is involved in polarized cell migration and cerebral cortical developmentKresimir Letinic
Department of Neurobiology and Kavli Institute for Neuroscience, 333 Cedar Street, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 106:11342-7. 2009..Thus, our study supports the notion that polarized membrane traffic regulated by the exocyst is an essential component of cell migration and that its deficit may lead to cortical abnormalities involving cortical neuronal malpositioning... - 3-D reconstruction of microtubules from multi-angle total internal reflection fluorescence microscopy using Bayesian frameworkQian Yang
Department of Electrical Engineering, Yale University, New Haven, CT 06511, USA
IEEE Trans Image Process 20:2248-59. 2011..The presented method has the potential to provide a reliable tool for 3-D reconstruction and tracking of microtubules... - Segmentation and 3D reconstruction of microtubules in total internal reflection fluorescence microscopy (TIRFM)Stathis Hadjidemetriou
Departments of Diagnostic Radiology and Biomedical Engineering, New Haven, CT 06520, USA
Med Image Comput Comput Assist Interv 8:761-9. 2005..The segments are described via Hamilton-Jacobi equations. Subsequently, the algorithm performs a limited reconstruction of the microtubules in 3D. Last, we evaluate our method with phantom as well as real TIRFM images of living cells... - Deciphering subcellular processes in live imaging datasets via dynamic probabilistic networksKresimir Letinic
Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA
Bioinformatics 26:2029-36. 2010.... - The tyrosine kinase activity of c-Src regulates actin dynamics and organization of podosomes in osteoclastsOlivier Destaing
Department of Orthopaedics, Yale University School of Medicine, New Haven, CT 06520, USA
Mol Biol Cell 19:394-404. 2008..Thus, Src is an essential regulator of podosome structure, dynamics and organization... - Par3 functions in the biogenesis of the primary cilium in polarized epithelial cellsJeff Sfakianos
Department of Cell Biology, Ludwig Institute for Cancer Research, New Haven, CT 06520
J Cell Biol 179:1133-40. 2007..Thus, the Par complex likely serves as an adaptor that couples the vectorial movement of at least a subset of membrane proteins to microtubule-dependent transport during ciliogenesis... - Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stagesRushika M Perera
Department of Cell Biology, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 103:19332-7. 2006..These results raise the possibility that dynamic phosphoinositide metabolism may occur throughout the lifetime of a CCP... - Motion tracking of the outer tips of microtubulesStathis Hadjidemetriou
Department of Biomedical Engineering and Diagnostic Radiology, Yale University, New Haven, CT 06520, USA
Med Image Anal 12:689-702. 2008..Last, we evaluate our method with phantom as well as real data. The real data show fluorescently tagged living cells imaged with epifluorescent microscopy or confocal microscopy... - Phosphatidylinositol phosphate kinase type I gamma regulates dynamics of large dense-core vesicle fusionLiang Wei Gong
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
Proc Natl Acad Sci U S A 102:5204-9. 2005..These results provide direct genetic evidence for a key role of phosphatidylinositol-4,5-bisphosphate synthesis in the regulation of large dense-core vesicle fusion dynamics... - Golgi biogenesis in Toxoplasma gondiiLaurence Pelletier
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Nature 418:548-52. 2002..Our results indicate that new Golgi grow by autonomous duplication and raise the possibility that the Golgi is a paired structure that is analogous to centrioles... - Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membraneAmy Chow
Department of Cell Biology and Section of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, Connecticut 06520-8002, USA
Nature 418:988-94. 2002..We show that on stimulation, dendritic cells generate tubules from lysosomal compartments that go on to fuse directly with the plasma membrane... - Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imagingWei Hua
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520, USA
J Cell Biol 172:1035-44. 2006.... - Overlapping events with application to image sequencesGuillermo Ayala
Departamento de EstadÃstica e Investigación Operativa, University of Valencia, Avda Vicent Andres Estelles, 46100 Burjasot, Spain
IEEE Trans Pattern Anal Mach Intell 28:1707-12. 2006..Our results show that the proposed temporal Boolean model is effective for obtaining information about dynamic processes which exhibit short-lived, but spatially and temporally overlapping events... - Measuring spatiotemporal dependencies in bivariate temporal random sets with applications to cell biologyEster Diaz
Department of Computer Science, University of Valencia, Burjasot, Spain
IEEE Trans Pattern Anal Mach Intell 30:1659-71. 2008.... - Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola- and F-actin-rich domainsJaime Millan
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
Nat Cell Biol 8:113-23. 2006.... - Spatio-temporal analysis of constitutive exocytosis in epithelial cellsRafael Sebastian
Departamento de Informatica, Universidad de Valencia, Dr Moliner, Burjassot, Spain
IEEE/ACM Trans Comput Biol Bioinform 3:17-32. 2006..Our novel approach could be translated to other studies of membrane trafficking in health and diseases such as diabetes... - Selective delivery of secretory cargo in Golgi-derived carriers of nonepithelial cellsAmin Rustom
Department of Neurobiology, Interdisciplinary Center of Neuroscience, University of Heidelberg, INF 364, Germany
Traffic 3:279-88. 2002..These findings suggest that nonepithelial cells, like their epithelial counterparts, possess a sorting machinery capable of selective packaging of secretory cargo into distinct types of carriers...