PATRICK M SUNG

Summary

Affiliation: Yale University
Country: USA

Publications

  1. ncbi request reprint Rad51 recombinase and recombination mediators
    Patrick Sung
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:42729-32. 2003
  2. doi request reprint Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
  3. pmc Recombination mediator and Rad51 targeting activities of a human BRCA2 polypeptide
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:11649-57. 2006
  4. ncbi request reprint Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase
    Rohit Prakash
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 280:7854-60. 2005
  5. pmc Role of the Rad52 amino-terminal DNA binding activity in DNA strand capture in homologous recombination
    Idina Shi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 284:33275-84. 2009
  6. ncbi request reprint Yeast recombination factor Rdh54 functionally interacts with the Rad51 recombinase and catalyzes Rad51 removal from DNA
    Peter Chi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:26268-79. 2006
  7. ncbi request reprint Purification and assays of Saccharomyces cerevisiae homologous recombination proteins
    Stephen Van Komen
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut, USA
    Methods Enzymol 408:445-63. 2006
  8. ncbi request reprint Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein
    Lumir Krejci
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 279:23193-9. 2004
  9. pmc ATP-dependent chromatin remodeling by the Saccharomyces cerevisiae homologous recombination factor Rdh54
    YoungHo Kwon
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 283:10445-52. 2008
  10. pmc Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2
    Eloïse Dray
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut, USA
    Nat Struct Mol Biol 17:1255-9. 2010

Collaborators

  • J E Haber
  • Hannah L Klein
  • Lumir Krejci
  • Michael G Sehorn
  • Ying Li
  • Yang Xie
  • Michal Hammel
  • Mary B Kroetz
  • Claudia Wiese
  • M A Macris
  • Eloïse Dray
  • David Schild
  • M Hochstrasser
  • Peter Chi
  • Changhyun Seong
  • Steven Raynard
  • YoungHo Kwon
  • Rohit Prakash
  • Joseph San Filippo
  • Sierra Colavito
  • Hengyao Niu
  • Valeria Busygina
  • Wendy Bussen
  • Stephen Van Komen
  • Idina Shi
  • Eric C Greene
  • Grzegorz Ira
  • Dongqing Liu
  • Lucy Lu
  • Simonne Longerich
  • Weixing Zhao
  • Uffe Mortensen
  • Hideo Tsubouchi
  • Akhilesh K Singh
  • Wilfried Kramer
  • Woo Hyun Chung
  • Zhu Zhu
  • Jürgen Scheller
  • Almas Papusha
  • Margaret Macris-Kiss
  • Olive Gleeson
  • Swee C L Hallwyl
  • Dana N Moses
  • Rodney Rothstein
  • Dominik Satory
  • G Shirleen Roeder
  • Yang Yang Ding
  • Binwei Song
  • Amom Ruhikanta Meetei
  • Iben Plate
  • Idina Y Shi
  • Galina V Petukhova
  • Dong Hyun Roh
  • Anastasiya Epshtein
  • Julia Etchin
  • Isabel Lam
  • Stefan Sigurdsson
  • Kirsten Anke Schürer

Detail Information

Publications33

  1. ncbi request reprint Rad51 recombinase and recombination mediators
    Patrick Sung
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:42729-32. 2003
  2. doi request reprint Mechanism of eukaryotic homologous recombination
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Annu Rev Biochem 77:229-57. 2008
    ..Recent progress on elucidating the mechanisms of action of Rad51 and Dmc1 and their cohorts of ancillary factors is reviewed here...
  3. pmc Recombination mediator and Rad51 targeting activities of a human BRCA2 polypeptide
    Joseph San Filippo
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:11649-57. 2006
    ..The results thus clarify the role of BRCA2 in Rad51-dependent DNA recombination and repair, and the experimental strategies described herein should be valuable for systematically deciphering this BRCA2 function...
  4. ncbi request reprint Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase
    Rohit Prakash
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 280:7854-60. 2005
    ..These results, thus, establish Mph1 as an ATP-dependent DNA helicase, and the availability of purified Mph1 should facilitate efforts at deciphering the role of this protein in homologous recombination and mutation avoidance...
  5. pmc Role of the Rad52 amino-terminal DNA binding activity in DNA strand capture in homologous recombination
    Idina Shi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 284:33275-84. 2009
    ..These results provide evidence that DNA binding by the evolutionarily conserved amino terminus of Rad52 is needed for the capture of the second DNA end during homologous recombination...
  6. ncbi request reprint Yeast recombination factor Rdh54 functionally interacts with the Rad51 recombinase and catalyzes Rad51 removal from DNA
    Peter Chi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:26268-79. 2006
    ..Rad51 complex formation. The Rdh54 expression and purification procedures described here should facilitate the functional dissection of this DNA recombination/repair factor...
  7. ncbi request reprint Purification and assays of Saccharomyces cerevisiae homologous recombination proteins
    Stephen Van Komen
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut, USA
    Methods Enzymol 408:445-63. 2006
    ..Continuing efforts with reconstituted systems that comprise yeast factors will undoubtedly continue to provide insights into the mechanistic intricacy of the homologous recombination machinery...
  8. ncbi request reprint Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein
    Lumir Krejci
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 279:23193-9. 2004
    ..These results highlight the importance of the ATP hydrolysisfueled DNA motor activity in SRS2 functions...
  9. pmc ATP-dependent chromatin remodeling by the Saccharomyces cerevisiae homologous recombination factor Rdh54
    YoungHo Kwon
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 283:10445-52. 2008
    ..These features of Rdh54 suggest a role of this protein factor in chromatin rearrangement during DNA recombination and repair...
  10. pmc Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2
    Eloïse Dray
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut, USA
    Nat Struct Mol Biol 17:1255-9. 2010
    ..Our results demonstrate the multifaceted role of PALB2 in chromosome damage repair. Because PALB2 mutations can cause cancer or Fanconi anemia, our findings shed light on the mechanism of tumor suppression in humans...
  11. pmc Hed1 regulates Rad51-mediated recombination via a novel mechanism
    Valeria Busygina
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Genes Dev 22:786-95. 2008
    ..These findings shed light on the function of Hed1 and, importantly, unveil a novel mechanism for the regulation of homologous recombination...
  12. pmc Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae
    Hengyao Niu
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 467:108-11. 2010
    ..Our reconstituted system should serve as a useful model for delineating the mechanistic intricacy of the DNA break resection process in eukaryotes...
  13. ncbi request reprint The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation
    Yang Xie
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    J Biol Chem 282:34176-84. 2007
    ..Our data reveal a novel mechanism of substrate targeting in which sumoylation of a protein can help trigger its subsequent ubiquitination by recruiting a SUMO-binding ubiquitin ligase...
  14. pmc Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase
    Peter Chi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Genes Dev 21:1747-57. 2007
    ..Thus, our results unveil a bipartite mechanism of Hop2-Mnd1 in homologous DNA pairing: stabilization of the Rad51 presynaptic filament and duplex DNA capture to enhance synaptic complex formation...
  15. ncbi request reprint Meiotic recombination: an affair of two recombinases
    Michael G Sehorn
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520 8024, USA
    Cell Cycle 3:1375-7. 2004
    ..The manner in which Rad51 and Dmc1 functionally cooperate in meiotic recombination remains to be determined...
  16. ncbi request reprint Biochemical characterization of the RECQ4 protein, mutated in Rothmund-Thomson syndrome
    Margaret A Macris
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar St, C130 Sterling Hall of Medicine, New Haven, CT 06520 8024, USA
    DNA Repair (Amst) 5:172-80. 2006
    ..Unlike the previously characterized members of the RecQ family, RECQ4 lacks a detectable DNA helicase activity...
  17. ncbi request reprint A double Holliday junction dissolvasome comprising BLM, topoisomerase IIIalpha, and BLAP75
    Steven Raynard
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 281:13861-4. 2006
    ..This function of the BLM-Topo IIIalpha-BLAP75 dissolvasome is likely indispensable for genome maintenance and cancer avoidance...
  18. ncbi request reprint Holliday junction processing activity of the BLM-Topo IIIalpha-BLAP75 complex
    Wendy Bussen
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 282:31484-92. 2007
    ....
  19. pmc Regulation of Rad51 recombinase presynaptic filament assembly via interactions with the Rad52 mediator and the Srs2 anti-recombinase
    Changhyun Seong
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 284:24363-71. 2009
    ..Moreover, our biochemical studies have helped identify A320V as a separation-of-function mutation in Rad51 with regards to a differential ablation of Rad52 interaction...
  20. ncbi request reprint Human meiotic recombinase Dmc1 promotes ATP-dependent homologous DNA strand exchange
    Michael G Sehorn
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Nature 429:433-7. 2004
    ..The DNA strand exchange activity of hDmc1 is probably indispensable for repair of DNA double-strand breaks during meiosis and for maintaining the ploidy of meiotic chromosomes...
  21. pmc DNA double-strand break processing: the beginning of the end
    Steven Raynard
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Genes Dev 22:2903-7. 2008
    ..Three recent studies, including work by Jackson and colleagues (pp. 2767- 2772) in the October 15, 2008, issue of Genes & Development, have begun to shed some light on the intricacy of this process...
  22. pmc Molecular anatomy of the recombination mediator function of Saccharomyces cerevisiae Rad52
    Changhyun Seong
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 283:12166-74. 2008
    ..Our results provide a mechanistic framework for rationalizing the multi-faceted role of Rad52 in recombination and DNA repair...
  23. pmc Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination
    Rohit Prakash
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Genes Dev 23:67-79. 2009
    ..Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Overall, the results from our analyses suggest a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks...
  24. pmc Functional interactions of meiotic recombination factors Rdh54 and Dmc1
    Peter Chi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    DNA Repair (Amst) 8:279-84. 2009
    ..The biochemical systems established in this study should be useful for the continuing dissection of the action mechanism of Rdh54 and Dmc1...
  25. ncbi request reprint Roles of ATP binding and ATP hydrolysis in human Rad51 recombinase function
    Peter Chi
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    DNA Repair (Amst) 5:381-91. 2006
    ..These results help form the basis for understanding the functions of ATP binding and ATP hydrolysis in hRad51-mediated recombination reactions...
  26. pmc FANCI binds branched DNA and is monoubiquitinated by UBE2T-FANCL
    Simonne Longerich
    Department of Molecular Biophysics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 284:23182-6. 2009
    ..We also demonstrate that FANCI can be ubiquitinated on Lys-523 by the UBE2T-FANCL pair in vitro. These findings should facilitate future efforts directed at elucidating molecular aspects of the FA pathway...
  27. pmc Synergistic action of the Saccharomyces cerevisiae homologous recombination factors Rad54 and Rad51 in chromatin remodeling
    YoungHo Kwon
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    DNA Repair (Amst) 6:1496-506. 2007
    ..These results suggest that Rad54-mediated chromatin remodeling coincides with DNA homology search by the Rad51 presynaptic filament and that this process is facilitated by an interaction of Rad54 with histone H3...
  28. pmc Assay for human Rad51-mediated DNA displacement loop formation
    Steven Raynard
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Cold Spring Harb Protoc 2009:pdb.prot5120. 2009
    ..This reconstituted system provides researchers a biochemical means to dissect the mechanisms of the homologous recombination machinery...
  29. pmc Multiplicity of DNA end resection machineries in chromosome break repair
    Hengyao Niu
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Genes Dev 23:1481-6. 2009
    ..Sinha and colleagues also demonstrated that the DNA end-binding protein complex Ku regulates the activity of AdnAB...
  30. ncbi request reprint Mechanism of homologous recombination: mediators and helicases take on regulatory functions
    Patrick Sung
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Nat Rev Mol Cell Biol 7:739-50. 2006
    ..Defects in BLM are also associated with the cancer phenotype. The past several years have witnessed dramatic advances in our understanding of the mechanism and regulation of HR...
  31. pmc Promotion and regulation of homologous recombination by DNA helicases
    Sierra Colavito
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar St, SHM C130, New Haven, CT 06520 8024, USA
    Methods 51:329-35. 2010
    ..These regulatory pathways function in parallel to promote the usage of the genome-preserving synthesis-dependent strand annealing HR pathway or otherwise suppress crossover formation...
  32. pmc Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption
    Sierra Colavito
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Nucleic Acids Res 37:6754-64. 2009
    ..These results highlight the importance of Rad51 interaction in the anti-recombinase function of Srs2, and provide evidence that this Srs2 function can be uncoupled from its helicase activity...
  33. pmc Functional role of BLAP75 in BLM-topoisomerase IIIalpha-dependent holliday junction processing
    Steven Raynard
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 283:15701-8. 2008
    ..This result provides proof that the functional integrity of the BTB complex is contingent upon the interaction of BLAP75 with Topo IIIalpha...

Research Grants48

  1. Molecular Basis of BRCA2-mediated DNA Repair and Cancer Avoidance
    Patrick Sung; Fiscal Year: 2007
    ..Our studies are therefore highly germane for cancer etiology, as the molecular information derived from them can be exploited to advance cancer diagnosis, prognosis and treatment. ..
  2. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2010
    ..The results from our research endeavors have direct relevance to understanding the role of homologous recombination in cancer suppression. ..
  3. BLM-mediated Homologous Recombination Regulation and Tumor Suppression
    Patrick Sung; Fiscal Year: 2007
    ..The results from our research endeavors will shed mechanistic light on BLM-dependent control mechanisms of HR and will provide an explanation for the genome instability and cancer susceptibility in BS patients. ..
  4. Homology-directed Repair of DNA Breaks in Humans
    Patrick Sung; Fiscal Year: 2007
    ..The results should shed some light on the mechanistic underpinnings of the homologous recombination machinery in human cells and will have important implications for cancer etiology and prevention. ..
  5. Role of Rad51 Paralogs in Mammalian DNA Repair
    Patrick Sung; Fiscal Year: 2006
    ..The studies should shed some light on the biological functions of the Rad51 paralogs and contribute to deciphering the mechanism of homologous recombinational repair in higher eukaryotic cells. ..
  6. FORMATION AND RESOLUTION OF RECOMBINATION INTERMEDIATES
    Patrick Sung; Fiscal Year: 2006
    ..The studies should provide insights about the mechanisms of the major recombination pathways in eukaryotic cells. ..
  7. Molecular Basis of BRCA2-mediated DNA Repair and Cancer Avoidance
    Patrick Sung; Fiscal Year: 2011
    ..Our studies are therefore highly germane for cancer etiology, as the molecular information derived from them can be exploited to advance cancer diagnosis, prognosis and treatment. ..
  8. FORMATION AND RESOLUTION OF RECOMBINATION INTERMEDIATES
    Patrick Sung; Fiscal Year: 2010
    ..For these reasons, the studies outlined in the renewal application have direct relevance to cancer biology and public health. ..
  9. BLM-mediated Homologous Recombination Regulation and Tumor Suppression
    Patrick Sung; Fiscal Year: 2010
    ..The results from our research endeavors will shed mechanistic light on BLM-dependent control mechanisms of HR and will provide an explanation for the genome instability and cancer susceptibility in BS patients. ..
  10. FORMATION AND RESOLUTION OF RECOMBINATION INTERMEDIATES
    Patrick Sung; Fiscal Year: 2009
    ..For these reasons, the studies outlined in the renewal application have direct relevance to cancer biology and public health. ..
  11. DNA Repair Genes and Proteins of the RAD52 group
    Patrick Sung; Fiscal Year: 2009
    ..abstract_text> ..
  12. YEAST DNA REPAIR GENES AND PROTEINS OF THE RAD52 GROUP
    Patrick Sung; Fiscal Year: 1999
    ..These proposed studies represent the starting point in dissecting the molecular mechanism of homologous recombination and repair of chromosomal breakage in eukaryotes. ..
  13. FORMATION AND RESOLUTION OF RECOMBINATION INTERMEDIATES
    Patrick Sung; Fiscal Year: 2002
    ..The Msh4 and Msh5 proteins will be purified and biochemical studies will be carried out to test the hypothesis that they interact with the Holliday structure and with other recombination intermediates. ..
  14. YEAST DNA REPAIR GENES AND PROTEINS OF THE RAD52 GROUP
    Patrick Sung; Fiscal Year: 2004
    ..The results from these studies will be important for understanding the mechanism of DNA double-strand break re air b recombination in eukaryotes including humans. ..