K C Slep
Affiliation: Yale University
- Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 AK C Slep
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511, USA
Nature 409:1071-7. 2001..Effector binding to a nucleotide-dependent site on alpha(t) sequesters PDEgamma residues implicated in PDE inhibition, and potentiates recruitment of RGS9 for hydrolytic transition state stabilization and concomitant signal termination...
- Sst2 is a GTPase-activating protein for Gpa1: purification and characterization of a cognate RGS-Galpha protein pair in yeastD M Apanovitch
Department of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
Biochemistry 37:4815-22. 1998..These experiments represent the first biochemical characterization of Gpa1 and Sst2, and provide a molecular basis for their well-established biological roles in signaling and desensitization...
- Prediction and confirmation of a site critical for effector regulation of RGS domain activityM E Sowa
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Nat Struct Biol 8:234-7. 2001..These results demonstrate the effectiveness of ET for identifying binding sites and efficiently focusing mutational studies on their key residues, thereby linking raw sequence and structure data to functional information...