Affiliation: Yale University
Santos Sacchi J. The speed limit of outer hair cell electromechanical activity. HNO. 2019;67:159-164 pubmed publisher
..Nevertheless, it is likely that the reduced magnitude of electromotility is sufficient to drive cochlear amplification at high frequencies. ..
Santos Sacchi J, Tan W. The Frequency Response of Outer Hair Cell Voltage-Dependent Motility Is Limited by Kinetics of Prestin. J Neurosci. 2018;38:5495-5506 pubmed publisher
..These observations highlight potential difficulties in providing sufficient amplification beyond a cutoff frequency near 20 kHz. Nevertheless, observed trade-offs in eM gain-bandwith may sustain cochlear amplification across frequency. ..
Song L, Santos Sacchi J. An electrical inspection of the subsurface cisternae of the outer hair cell. Biophys J. 2015;108:568-77 pubmed publisher
..Finally, a cable model of the OHC, which can match our data, indicates that the SSC poses a minimal barrier to current flow across it, thereby facilitating rapid delivery of voltage excitation to the prestin-embedded lateral membrane. ..
McKay S, Yan W, Nouws J, Thormann M, Raimundo N, Khan A, et al
. Auditory Pathology in a Transgenic mtTFB1 Mouse Model of Mitochondrial Deafness. Am J Pathol. 2015;185:3132-40 pubmed publisher
..The relevance of these findings to human A1555G patients and the potential therapeutic value of reducing AMPK activity are discussed. ..
Santos Sacchi J, Song L. Chloride Anions Regulate Kinetics but Not Voltage-Sensor Qmax of the Solute Carrier SLC26a5. Biophys J. 2016;110:2551-2561 pubmed publisher
Santos Sacchi J. Cochlear mechanics: no shout but a twist in the absence of prestin. Curr Biol. 2008;18:R304-6 pubmed publisher
..Recent evidence indicates that the molecular motor that drives this amplification, prestin, may do more than boogie. ..
Song L, Santos Sacchi J. A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells. Methods Mol Biol. 2016;1427:501-12 pubmed publisher
..We use the software jClamp since it is tailored to capacitance measurement. Here we detail steps that we use to measure NLC. The walk through includes isolation of guinea pig OHCs, building voltage commands, recording, and analysis. ..