Thomas Pollard

Summary

Affiliation: Yale University
Country: USA

Publications

  1. pmc Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast
    Vladimir Sirotkin
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 170:637-48. 2005
  2. pmc UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II
    Matthew Lord
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 167:315-25. 2004
  3. pmc Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 174:391-402. 2006
  4. pmc The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 161:875-87. 2003
  5. pmc No question about exciting questions in cell biology
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America Department of Cell Biology, Yale University, New Haven, Connecticut, United States of America
    PLoS Biol 11:e1001734. 2013
  6. pmc Characterization of two classes of small molecule inhibitors of Arp2/3 complex
    B J Nolen
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Nature 460:1031-4. 2009
  7. pmc Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring
    Qian Chen
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 195:485-98. 2011
  8. pmc Tension modulates actin filament polymerization mediated by formin and profilin
    Naomi Courtemanche
    Departments of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Proc Natl Acad Sci U S A 110:9752-7. 2013
  9. pmc Take advantage of time in your experiments: a guide to simple, informative kinetics assays
    Thomas D Pollard
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 24:1103-10. 2013
  10. pmc Political advocacy by the American Society for Cell Biology and its partners
    Thomas D Pollard
    Departments of Molecular, Cellular, and Developmental Biology, Molecular Biophysics and Biochemistry, and Cell Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 23:4171-4. 2012

Research Grants

  1. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2005
  2. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2005
  3. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2006
  4. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2006
  5. Structure and Function of Arp 2/3 Complex
    Thomas Pollard; Fiscal Year: 2007
  6. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2007
  7. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2007
  8. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2009
  9. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2009
  10. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas D Pollard; Fiscal Year: 2010

Collaborators

Detail Information

Publications67

  1. pmc Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast
    Vladimir Sirotkin
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 170:637-48. 2005
    ..Genetic dependencies of patch formation suggest that patch formation involves cross talk between Myo1p and Wsp1p/Vrp1p pathways...
  2. pmc UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II
    Matthew Lord
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 167:315-25. 2004
    ..In contrast, Myo2 with certain temperature-sensitive forms of Cdc4p has normal motility, so these mutations compromise other functions of Cdc4p required for cytokinesis...
  3. pmc Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 174:391-402. 2006
    ..Their absence delays late steps in cytokinesis, including septum formation and cell separation...
  4. pmc The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 161:875-87. 2003
    ..These properties explain why contractile ring assembly requires both formin and profilin and why viability depends on the ability of profilin to bind both actin and poly-l-proline...
  5. pmc No question about exciting questions in cell biology
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, United States of America Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, United States of America Department of Cell Biology, Yale University, New Haven, Connecticut, United States of America
    PLoS Biol 11:e1001734. 2013
    ..Many big questions remain to be answered before we reach this goal of understanding cellular dynamics...
  6. pmc Characterization of two classes of small molecule inhibitors of Arp2/3 complex
    B J Nolen
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Nature 460:1031-4. 2009
    ..Two inhibitors with different mechanisms of action provide a powerful approach for studying the Arp2/3 complex in living cells...
  7. pmc Actin filament severing by cofilin is more important for assembly than constriction of the cytokinetic contractile ring
    Qian Chen
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 195:485-98. 2011
    ....
  8. pmc Tension modulates actin filament polymerization mediated by formin and profilin
    Naomi Courtemanche
    Departments of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Proc Natl Acad Sci U S A 110:9752-7. 2013
    ..Thus, physical forces strongly influence actin assembly by formin Bni1p...
  9. pmc Take advantage of time in your experiments: a guide to simple, informative kinetics assays
    Thomas D Pollard
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 24:1103-10. 2013
    ..These "transient-state" methods not only differ in concept from traditional approaches used to analyze enzyme reactions at steady state, but they are also applicable to learning about the dynamics of any biological process...
  10. pmc Political advocacy by the American Society for Cell Biology and its partners
    Thomas D Pollard
    Departments of Molecular, Cellular, and Developmental Biology, Molecular Biophysics and Biochemistry, and Cell Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 23:4171-4. 2012
    ..I celebrate how good leadership and an effective staff enabled its energetic volunteer organization to have an impact, but I also ask how the effort can be made more successful...
  11. doi request reprint The obligation for biologists to commit to political advocacy
    Thomas D Pollard
    Departments of Molecular Cellular and Developmental Biology, Yale University, PO Box 208103, New Haven, CT 06520 8103, USA
    Cell 151:239-43. 2012
    ..I explain here why all scientists should feel obligated to do their part to support the community by advocating for the benefits of government investments in scientific research and training...
  12. ncbi request reprint Structure and function of the Arp2/3 complex
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, PO Box 208103, New Haven, CT 06520 8103, USA
    Curr Opin Struct Biol 12:768-74. 2002
    ..Crystal and cryo-electron microscopic structures of the Arp2/3 complex and branch junctions have clarified extensive biochemical data on the mechanism of this process...
  13. ncbi request reprint Regulation of actin filament assembly by Arp2/3 complex and formins
    Thomas D Pollard
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Annu Rev Biophys Biomol Struct 36:451-77. 2007
    ..Formins nucleate and support the elongation of unbranched actin filaments for cytokinesis and various types of actin filament bundles. Formins associate processively with the fast-growing ends of filaments and protect them from capping...
  14. pmc Progress towards understanding the mechanism of cytokinesis in fission yeast
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Cell Biology, Yale University, New Haven, CT 06520 8103, USA
    Biochem Soc Trans 36:425-30. 2008
    ..As a cell enters anaphase, actin filaments grow from formin Cdc12p in these nodes. Myosin captures actin filaments from adjacent nodes and pulls intermittently to condense the nodes into a contractile ring...
  15. pmc A guide to simple and informative binding assays
    Thomas D Pollard
    Departments of Molecular Cellular and Developmental Biology, of Molecular Biophysics and Biochemistry and of Cell Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 21:4061-7. 2010
    ..In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments...
  16. ncbi request reprint Mathematical models and simulations of cellular processes based on actin filaments
    Thomas D Pollard
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Biol Chem 284:5433-7. 2009
    ....
  17. pmc Actin, a central player in cell shape and movement
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 326:1208-12. 2009
    ..Comparisons of quantitative measurements of reactions in live cells with computer simulations of mathematical models will also help generate meaningful insights...
  18. ncbi request reprint Life scientists and politics in the United States
    T D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Nat Rev Mol Cell Biol 2:929-31. 2001
    ..Life scientists, acting through scientific societies and the National Academies of Science, also have a strong influence on political debates about issues with a scientific basis...
  19. pmc Functional genomics of cell morphology using RNA interference: pick your style, broad or deep
    Thomas D Pollard
    Departments of Molecular Cellular and Developmental Biology and Cell Biology, Yale University, New Haven, CT 06520, USA
    J Biol 2:25. 2003
    ..One broad survey of nearly 1,000 proteins and three studies focused on cytoskeletal and motor proteins have identified key proteins essential for these processes in animal cells...
  20. ncbi request reprint The cytoskeleton, cellular motility and the reductionist agenda
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven Connecticut 06520 8103, USA
    Nature 422:741-5. 2003
    ..Much of the molecular machinery for motility evolved in early eukaryotes, so a limited set of general principles can explain the motility of most cells...
  21. ncbi request reprint Cellular motility driven by assembly and disassembly of actin filaments
    Thomas D Pollard
    Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 112:453-65. 2003
    ..Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation...
  22. ncbi request reprint Ray Rappaport chronology: Twenty-five years of seminal papers on cytokinesis in the Journal of Experimental Zoology
    Thomas D Pollard
    Departments of Molecular Cellular and Developmental Biology and Cell Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Exp Zool A Comp Exp Biol 301:9-14. 2004
  23. pmc The role of the FH1 domain and profilin in formin-mediated actin-filament elongation and nucleation
    Aditya S Paul
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8103, USA
    Curr Biol 18:9-19. 2008
    ..The mechanism by which this effect is achieved is not well understood...
  24. ncbi request reprint Assembly mechanism of the contractile ring for cytokinesis by fission yeast
    Dimitrios Vavylonis
    Department of Chemical Engineering, Columbia University, New York, NY 10027, USA
    Science 319:97-100. 2008
    ..Analysis of node motions and numerical simulations supported a mechanism whereby transient connections are established when myosins in one node capture and exert force on actin filaments growing from other nodes...
  25. ncbi request reprint Single molecule kinetic analysis of actin filament capping. Polyphosphoinositides do not dissociate capping proteins
    Jeffrey R Kuhn
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 282:28014-24. 2007
    ..PPIs binding to this site might interfere sterically with capping, but this site would be inaccessible when CP is bound to the end of a filament...
  26. pmc Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy
    Ikuko Fujiwara
    Department of Molecular Biology, Yale University, New Haven, CT 06520 8103, USA
    Proc Natl Acad Sci U S A 104:8827-32. 2007
    ..Our observations of single actin filaments provide clues about the origins of the difference in the critical concentration at the two ends of actin filaments in the presence of ATP...
  27. pmc Nucleotide-mediated conformational changes of monomeric actin and Arp3 studied by molecular dynamics simulations
    Paul Dalhaimer
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Mol Biol 376:166-83. 2008
    ..In sum, these simulations show that subtle differences in structures of actin family proteins have profound effects on their nucleotide-driven behavior...
  28. ncbi request reprint Twinfilin is an actin-filament-severing protein and promotes rapid turnover of actin structures in vivo
    James B Moseley
    Department of Biology and The Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA
    J Cell Sci 119:1547-57. 2006
    ..Together, our data suggest that twinfilin coordinates filament severing and monomer sequestering at sites of rapid actin turnover and is controlled by multiple regulatory inputs...
  29. pmc Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex
    Brad J Nolen
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Cell 26:449-57. 2007
    ..Steric clashes with Arp3 appear to be responsible for intrinsic disorder of subdomains 1 and 2 of Arp2 in inactive Arp2/3 complex...
  30. doi request reprint Pathway of actin filament branch formation by Arp2/3 complex
    Christopher C Beltzner
    Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Biol Chem 283:7135-44. 2008
    ..15 s(-1). Kinetic parameters determined in this study constrain the process of actin filament branch formation during cellular motility to one main pathway...
  31. pmc Leiomodin is an actin filament nucleator in muscle cells
    David Chereau
    Boston Biomedical Research Institute, Watertown, MA 02472, USA
    Science 320:239-43. 2008
    ..Knocking down leiomodin severely compromised sarcomere assembly in cultured muscle cells, which suggests a role for leiomodin in the nucleation of tropomyosin-decorated filaments in muscles...
  32. pmc EPLIN regulates actin dynamics by cross-linking and stabilizing filaments
    Raymond S Maul
    Department of Medicine, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA
    J Cell Biol 160:399-407. 2003
    ..Reduced expression of EPLIN may contribute to the motility of invasive tumor cells...
  33. ncbi request reprint Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilin
    Laurent Blanchoin
    Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 41:597-602. 2002
    ..Profilin must move from this site at rates much faster than it dissociates from monomers (4 s(-1)). ATP hydrolysis is not required for this movement...
  34. ncbi request reprint Xenopus actin-interacting protein 1 (XAip1) enhances cofilin fragmentation of filaments by capping filament ends
    Kyoko Okada
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 277:43011-6. 2002
    ..We conclude that XAip1 caps the barbed end of filaments severed by cofilin. This capping blocks annealing and depolymerization and allows more extensive severing by cofilin...
  35. ncbi request reprint Formins initiate new actin filaments
    Thomas D Pollard
    Nat Cell Biol 4:E191. 2002
  36. ncbi request reprint Arp2/3 plays no role in muscle contraction
    Thomas D Pollard
    Nature 418:915. 2002
  37. pmc The structural basis of actin filament branching by the Arp2/3 complex
    Isabelle Rouiller
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    J Cell Biol 180:887-95. 2008
    ..These data provide a rationale for why branch formation requires cooperative interactions among the Arp2/3 complex, nucleation-promoting factors, an actin monomer, and the mother filament...
  38. ncbi request reprint Control of the assembly of ATP- and ADP-actin by formins and profilin
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 124:423-35. 2006
    ..These results suggest that diverse formins are mechanistically similar, but the rates of particular assembly steps vary...
  39. pmc Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit
    Brad J Nolen
    Departments of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    J Biol Chem 283:26490-8. 2008
    ..Truncation of the N terminus of ARPC5, the smallest subunit in the complex, increased the yield of Arp2Delta Arp2/3 complex during purification but did not compromise nucleation activity of the full Arp2/3 complex...
  40. pmc Yeast UCS proteins promote actomyosin interactions and limit myosin turnover in cells
    Matthew Lord
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA
    Proc Natl Acad Sci U S A 105:8014-9. 2008
    ..Thus, a fraction of this yeast myosin can fold de novo in the absence of UCS proteins, but UCS proteins promote myosin stability and interactions with actin...
  41. pmc Influence of phalloidin on the formation of actin filament branches by Arp2/3 complex
    Rachel E Mahaffy
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Biochemistry 47:6460-7. 2008
    ..Therefore, the appearance of more branches in samples treated with rhodamine-phalloidin arises from multiple influences of the peptide on both the formation and dissociation of branches...
  42. ncbi request reprint John Heuser's contributions to the visualization of the actin cytoskeleton by electron microscopy
    Thomas D Pollard
    Eur J Cell Biol 83:253-5. 2004
  43. pmc Myosin Va maneuvers through actin intersections and diffuses along microtubules
    M Yusuf Ali
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA
    Proc Natl Acad Sci U S A 104:4332-6. 2007
    ..These features of myoVa may help ensure efficient cargo delivery from the cell center to the periphery...
  44. pmc Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Mol Biol Cell 16:2313-24. 2005
    ..Given that capping protein arrives at the division site 20 min later than Cdc12p, capping protein may slowly replace Cdc12p on filament barbed ends in preparation for filament disassembly during ring constriction...
  45. ncbi request reprint Progressing actin: Formin as a processive elongation machine
    David R Kovar
    Nat Cell Biol 6:1158-9. 2004
  46. ncbi request reprint Assembly of Acanthamoeba myosin-II minifilaments. Model of anti-parallel dimers based on EM and X-ray diffraction of 2D and 3D crystals
    Kirsi Turbedsky
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 345:363-73. 2005
    ..On the basis of the low-resolution 3D structure, biochemical constraints, and X-ray diffraction data, we propose a model for myosin interactions in the anti-parallel dimer of coiled-coils that guide the first step of myosin-II assembly...
  47. ncbi request reprint Assembly of Acanthamoeba myosin-II minifilaments. Definition of C-terminal residues required to form coiled-coils, dimers, and octamers
    Kirsi Turbedsky
    Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 345:351-61. 2005
    ..These results support a model in which hydrophobic as well as electrostatic interactions control the register between myosin-II coiled-coils and guide sequential steps of dimerization that generate stable, octameric mini-filaments...
  48. pmc Real-time measurements of actin filament polymerization by total internal reflection fluorescence microscopy
    Jeffrey R Kuhn
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Biophys J 88:1387-402. 2005
    ..56 microM(-1) s(-1) and 0.19 s(-1). When vitamin D binding protein sequesters all free monomers, ADP-actin dissociates from barbed ends at 1.4 s(-1) and from pointed ends at 0.16 s(-1) regardless of buffer nucleotide...
  49. pmc Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP
    Brad J Nolen
    Departments of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Proc Natl Acad Sci U S A 101:15627-32. 2004
    ..These structures provide the an opportunity to compare all nucleotide-binding states in an actin-related protein and give insights into the function of both the Arp2/3 complex and actin...
  50. ncbi request reprint Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse species
    Christopher C Beltzner
    Department of Molecular Cellular and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520 8103, USA
    J Mol Biol 336:551-65. 2004
    ..Other conserved residues were used with experimental constraints to propose how residues on the subunits ARPC1, ARPC2, ARPC4 and ARPC5 might interact with the mother filament at branch junctions...
  51. ncbi request reprint Cellular motility powered by actin filament assembly and disassembly
    Thomas D Pollard
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, USA
    Harvey Lect 98:1-17. 2002
  52. ncbi request reprint Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Dev Cell 5:723-34. 2003
    ..Ring contraction and disassembly begin 37 min after SPB separation. This spatial and temporal hierarchy provides the framework for analysis of molecular mechanisms...
  53. pmc Visualizing Arp2/3 complex activation mediated by binding of ATP and WASp using structural mass spectrometry
    Janna G Kiselar
    Case Center for Proteomics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 104:1552-7. 2007
    ....
  54. pmc Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast
    Matthew Lord
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Mol Biol Cell 16:5346-55. 2005
    ..Myo1p is the first example of a myosin whose cellular function does not require a catalytic motor domain revealing a novel mechanism of action for budding yeast myosin-II independent of actin binding and ATPase activity...
  55. ncbi request reprint Counting cytokinesis proteins globally and locally in fission yeast
    Jian Qiu Wu
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 310:310-4. 2005
    ..Proteins concentrated up to 100 times in contractile rings and 7500 times in spindle pole bodies at certain times in the cell cycle. This approach can be used to measure the global and local concentrations of any fusion protein...
  56. pmc Model of formin-associated actin filament elongation
    Dimitrios Vavylonis
    Department of Chemical Engineering, Columbia University, New York, New York 10027, USA
    Mol Cell 21:455-66. 2006
    ..The mechanism does not require ATP hydrolysis by polymerized actin, but we cannot exclude that formins accelerate hydrolysis...
  57. ncbi request reprint A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex
    Sanjay C Panchal
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Biol 10:591-8. 2003
    ..Key residues in this motif are buried through contacts with the GTPase binding domain in the autoinhibited structure of WASP and N-WASP, indicating that sequestration of these residues is an important aspect of autoinhibition...
  58. pmc Reconstitution of the transition from lamellipodium to filopodium in a membrane-free system
    Lior Haviv
    Department of Chemical Engineering, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Proc Natl Acad Sci U S A 103:4906-11. 2006
    ..This mechanism may apply to the transition from lamellipodia to filopodia in vivo...
  59. doi request reprint A malaria parasite formin regulates actin polymerization and localizes to the parasite-erythrocyte moving junction during invasion
    Jake Baum
    Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Cell Host Microbe 3:188-98. 2008
    ....
  60. ncbi request reprint Formins coming into focus
    Thomas D Pollard
    Departments of Molecular, Cellular and Developmental Biology, Cell Biology, and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Dev Cell 6:312-4. 2004
    ..Two new crystal structures published in Cell and Molecular Cell provide the first clues about how fascinating proteins called formins interact with actin filaments...
  61. ncbi request reprint Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin
    Ernesto Andrianantoandro
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Mol Cell 24:13-23. 2006
    ..These results establish a clear paradigm for actin turnover by cofilin in cells...
  62. pmc Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 101:14725-30. 2004
    ..However, profilin increased the rate of Bni1(FH1FH2)p-mediated barbed end elongation from 75% to 100% of full-speed...
  63. pmc Kinetics of the formation and dissociation of actin filament branches mediated by Arp2/3 complex
    Rachel E Mahaffy
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Biophys J 91:3519-28. 2006
    ..We conclude that the nucleotide on the mother filament does not affect the initial branching event but that branches are an order of magnitude more stable on the sides of new ATP- or ADP-P(i) filaments than on ADP-actin filaments...
  64. ncbi request reprint Intracellular localization and dynamics of myosin-II and myosin-IC in live Acanthamoeba by transient transfection of EGFP fusion proteins
    Hyun Hee Kong
    Structural Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    J Cell Sci 115:4993-5002. 2002
    ..EGFP-myosin-IC was spread throughout the cytoplasm but concentrated transiently around contractile vacuoles and macropinocytosis cups providing that the construct included both the head and a tail with the SH3 domain...
  65. ncbi request reprint Dynamic localization of myosin-I to endocytic structures in Acanthamoeba
    E Michael Ostap
    The Pennsylvania Muscle Institute and The Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104 6085, USA
    Cell Motil Cytoskeleton 54:29-40. 2003
    ....
  66. ncbi request reprint High affinity, paralog-specific recognition of the Mena EVH1 domain by a miniature protein
    Dasantila Golemi-Kotra
    Departments of Chemistry and Molecular, Cellular, Yale University, New Haven, Connecticut 06511, USA
    J Am Chem Soc 126:4-5. 2004
    ..Our results suggest that miniature proteins based on aPP may represent an excellent framework for the design of ligands that differentiate the roles of EVH1 domains in vitro and in vivo...
  67. ncbi request reprint A conversation with Thomas D. Pollard. Interview by K. Noelle Gracy
    Thomas D Pollard
    Biochem Biophys Res Commun 297:6-9. 2002

Research Grants34

  1. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2005
    ..The combination of genetics, biochemistry and microscopy of fission yeast offers great advantages for establishing the molecular pathways controlling actin assembly. ..
  2. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2005
    ..We will determine the mechanism of actin filament nucleation by formin proteins and use suppressor analysis to verify that Cdc12 is the (exclusive) poly-L-proline ligand for profilin in S. pombe. ..
  3. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2006
    ..We will determine the mechanism of actin filament nucleation by formin proteins and use suppressor analysis to verify that Cdc12 is the (exclusive) poly-L-proline ligand for profilin in S. pombe. ..
  4. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2006
    ..We will investigate how the SIN pathway triggers constriction of the contractile ring, explore the factors that limit the rate of constriction of contractile rings and determine how the contractile ring disassembles during constriction. ..
  5. Structure and Function of Arp 2/3 Complex
    Thomas Pollard; Fiscal Year: 2007
    ..abstract_text> ..
  6. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2007
    ..We will investigate how the SIN pathway triggers constriction of the contractile ring, explore the factors that limit the rate of constriction of contractile rings and determine how the contractile ring disassembles during constriction. ..
  7. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2007
    ..We will determine the mechanism of actin filament nucleation by formin proteins and use suppressor analysis to verify that Cdc12 is the (exclusive) poly-L-proline ligand for profilin in S. pombe. ..
  8. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2009
    ....
  9. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2009
    ..Movement of cells out of primary tumors is the chief cause of mortality in cancer. ..
  10. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas D Pollard; Fiscal Year: 2010
    ..Movement of cells out of primary tumors is the chief cause of mortality in cancer. ..
  11. Actin Myosin Interactions in Cell Motility
    Thomas D Pollard; Fiscal Year: 2010
    ..Our studies on fission yeast are revealing ancient mechanisms that evolved more than 1 billion years ago and are still used by animal cells. ..
  12. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas D Pollard; Fiscal Year: 2010
    ..Movement of cells out of primary tumors is the chief cause of mortality in cancer. ..
  13. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2004
    ..We will determine the mechanism of actin filament nucleation by formin proteins and use suppressor analysis to verify that Cdc12 is the (exclusive) poly-L-proline ligand for profilin in S. pombe. ..
  14. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2004
    ..The combination of genetics, biochemistry and microscopy of fission yeast offers great advantages for establishing the molecular pathways controlling actin assembly. ..
  15. ACTIN/MYOSIN INTERACTIONS IN CELL MOTILITY
    Thomas Pollard; Fiscal Year: 1999
    ....
  16. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 1999
    ..4) To test further a model in which alpha-actinin functions as a dynamic crosslinker of actin filaments in vitro. ..
  17. ACTIN/MYOSIN INTERACTIONS IN CELL MOTILITY
    Thomas Pollard; Fiscal Year: 2000
    ....
  18. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2000
    ..We are confident that general principles about molecular mechanisms in the actin cytoskeleton will emerge from this work. ..
  19. ACTIN/MYOSIN INTERACTIONS IN CELL MOTILITY
    Thomas Pollard; Fiscal Year: 2001
    ....
  20. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2001
    ..We are confident that general principles about molecular mechanisms in the actin cytoskeleton will emerge from this work. ..
  21. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2001
    ..We are confident that general principles about molecular mechanisms in the actin cytoskeleton will emerge from this work. ..
  22. Conference on Wiring and Plasticity of Nervous Systems
    Thomas Pollard; Fiscal Year: 2002
    ..We are confident that mutually beneficial insights will emerge from learning about each others work. ..
  23. ACTIN/MYOSIN INTERACTIONS IN CELL MOTILITY
    Thomas Pollard; Fiscal Year: 2001
    ....
  24. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2002
    ..We are confident that general principles about molecular mechanisms in the actin cytoskeleton will emerge from this work. ..
  25. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2002
    ..The combination of genetics, biochemistry and microscopy of fission yeast offers great advantages for establishing the molecular pathways controlling actin assembly. ..
  26. Actin Myosin Interactions in Cell Motility
    Thomas Pollard; Fiscal Year: 2003
    ..The combination of genetics, biochemistry and microscopy of fission yeast offers great advantages for establishing the molecular pathways controlling actin assembly. ..
  27. MOLECULAR MECHANISMS OF CELLULAR MOTILITY
    Thomas Pollard; Fiscal Year: 2003
    ..We are confident that general principles about molecular mechanisms in the actin cytoskeleton will emerge from this work. ..
  28. Actin Myosin Interactions in Cell Motility
    Thomas D Pollard; Fiscal Year: 2010
    ..We will investigate how the SIN pathway triggers constriction of the contractile ring, explore the factors that limit the rate of constriction of contractile rings and determine how the contractile ring disassembles during constriction. ..