ROBERT MACNAB

Summary

Affiliation: Yale University
Country: USA

Publications

  1. pmc Effects of lipoprotein biogenesis mutations on flagellar assembly in Salmonella
    Frank E Dailey
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Bacteriol 184:771-6. 2002
  2. ncbi request reprint A top runner of the flagellar world has unexpectedly gone
    Shin Ichi Aizawa
    Biochim Biophys Acta 1694:3-4. 2004
  3. ncbi request reprint The type III flagellar export specificity switch is dependent on FliK ruler and a molecular clock
    Nao Moriya
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    J Mol Biol 359:466-77. 2006
  4. ncbi request reprint Microbiology. Action at a distance--bacterial flagellar assembly
    R M Macnab
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 290:2086-7. 2000
  5. ncbi request reprint How bacteria assemble flagella
    Robert M Macnab
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Microbiol 57:77-100. 2003
  6. ncbi request reprint Domain organization and function of Salmonella FliK, a flagellar hook-length control protein
    Tohru Minamino
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 341:491-502. 2004
  7. ncbi request reprint Structural and functional analysis of the C-terminal cytoplasmic domain of FlhA, an integral membrane component of the type III flagellar protein export apparatus in Salmonella
    Yumiko Saijo-Hamano
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 343:457-66. 2004
  8. pmc Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery
    Jonathan L McMurry
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Bacteriol 186:7586-92. 2004
  9. ncbi request reprint Type III flagellar protein export and flagellar assembly
    Robert M Macnab
    Department of Molecular Biophysics and Biochemistry, Yale University, 0734, 266 Whitney Avenue, P O Box 208114, New Haven, CT 06520 8114, USA
    Biochim Biophys Acta 1694:207-17. 2004
  10. ncbi request reprint FlhB regulates ordered export of flagellar components via autocleavage mechanism
    Hedda U Ferris
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:41236-42. 2005

Research Grants

Collaborators

Detail Information

Publications20

  1. pmc Effects of lipoprotein biogenesis mutations on flagellar assembly in Salmonella
    Frank E Dailey
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Bacteriol 184:771-6. 2002
    ..We have isolated second-site mutants from an lgt(Ts) lpp(+) strain that grow but are nonflagellate at 42 degrees C. Thus, lipoprotein biogenesis is a factor that is important for flagellar assembly...
  2. ncbi request reprint A top runner of the flagellar world has unexpectedly gone
    Shin Ichi Aizawa
    Biochim Biophys Acta 1694:3-4. 2004
  3. ncbi request reprint The type III flagellar export specificity switch is dependent on FliK ruler and a molecular clock
    Nao Moriya
    Graduate School of Frontier Biosciences, Osaka University, Osaka 565 0871, Japan
    J Mol Biol 359:466-77. 2006
    ..We propose that FliK(N) acts as a flexible tape measure, but that hook length is also dependent on the hook elongation rate and a switch timing mechanism...
  4. ncbi request reprint Microbiology. Action at a distance--bacterial flagellar assembly
    R M Macnab
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 290:2086-7. 2000
    ....
  5. ncbi request reprint How bacteria assemble flagella
    Robert M Macnab
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Microbiol 57:77-100. 2003
    ..Exported substrates diffuse down a narrow channel in the growing structure and assemble at the distal end, often with the help of a capping structure...
  6. ncbi request reprint Domain organization and function of Salmonella FliK, a flagellar hook-length control protein
    Tohru Minamino
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 341:491-502. 2004
    ..The conformational flexibility of FliK(C) may play a role in interfering with switching at an inappropriate point of flagellar assembly...
  7. ncbi request reprint Structural and functional analysis of the C-terminal cytoplasmic domain of FlhA, an integral membrane component of the type III flagellar protein export apparatus in Salmonella
    Yumiko Saijo-Hamano
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Mol Biol 343:457-66. 2004
    ..Amino acid residues 328-351 of FlhA appear to be a relatively flexible linker between the transmembrane domain and FlhAC38K...
  8. pmc Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery
    Jonathan L McMurry
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    J Bacteriol 186:7586-92. 2004
    ..Affinity blotting showed that FlhA interacts with several other export apparatus membrane proteins. The implications of these findings are discussed, and a model of FlhA within the export apparatus is presented...
  9. ncbi request reprint Type III flagellar protein export and flagellar assembly
    Robert M Macnab
    Department of Molecular Biophysics and Biochemistry, Yale University, 0734, 266 Whitney Avenue, P O Box 208114, New Haven, CT 06520 8114, USA
    Biochim Biophys Acta 1694:207-17. 2004
    ....
  10. ncbi request reprint FlhB regulates ordered export of flagellar components via autocleavage mechanism
    Hedda U Ferris
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:41236-42. 2005
    ..Finally, we provide evidence via peptide analysis and FlhB cleavage variants that the tertiary structure of FlhB plays a significant role in cleavage. Based on these results, we propose that FlhB cleavage is an autocatalytic process...
  11. pmc Crystallization and preliminary X-ray analysis of the C-terminal cytoplasmic domain of FlhA, a membrane-protein subunit of the bacterial flagellar type III protein-export apparatus
    Yumiko Saijo-Hamano
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:599-602. 2005
    ..FlhAC38K was crystallized in an orthorhombic form, with unit-cell parameters a = 53.0, b = 93.1, c = 186.5 A. X-ray diffraction data from crystals of FlhAC and the SeMet derivative of FlhAC were collected to 2.9 and 3.2 A, respectively...
  12. pmc Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB
    John S Van Arnam
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    J Bacteriol 186:2495-8. 2004
    ..We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio...
  13. pmc Interactions of FliJ with the Salmonella type III flagellar export apparatus
    Gillian M Fraser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 185:5546-54. 2003
    ..We present a model summarizing these multiple interactions...
  14. ncbi request reprint Interactions among membrane and soluble components of the flagellar export apparatus of Salmonella
    Keng Zhu
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Biochemistry 41:9516-24. 2002
    ..FliH bound more weakly to FliI carrying the N-terminal double mutation R7C/L12P than it did to wild-type FliI, confirming the importance of the N terminus of FliI for its interaction with FliH...
  15. ncbi request reprint Molecular dissection of Salmonella FliH, a regulator of the ATPase FliI and the type III flagellar protein export pathway
    Bertha González-Pedrajo
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Mol Microbiol 45:967-82. 2002
    ..Based on these data, a model is presented for the domain construction and function of FliH in complex with FliI and FliJ...
  16. ncbi request reprint Structural properties of FliH, an ATPase regulatory component of the Salmonella type III flagellar export apparatus
    Tohru Minamino
    Protonic NanoMachine Project, ERATO, JST, 1 7 Hikaridai, Seika, Kyoto, Japan
    J Mol Biol 322:281-90. 2002
    ....
  17. pmc Substrate specificity classes and the recognition signal for Salmonella type III flagellar export
    Takanori Hirano
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Bacteriol 185:2485-92. 2003
    ..We conclude that the amino acid sequence of these proteins suffices to mediate their recognition and export...
  18. ncbi request reprint Substrate specificity of type III flagellar protein export in Salmonella is controlled by subdomain interactions in FlhB
    Gillian M Fraser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Mol Microbiol 48:1043-57. 2003
    ..With FlhB(P270A), cleavage was reduced but not abolished, and cells producing this protein were weakly motile, exported reduced amounts of flagellin and assembled polyhook filaments...
  19. pmc The ATPase FliI can interact with the type III flagellar protein export apparatus in the absence of its regulator, FliH
    Tohru Minamino
    Protonic NanoMachine Project, ERATO and Dynamic NanoMachine Project, ICORP, JST, Seika, Kyoto 619 0237, Japan
    J Bacteriol 185:3983-8. 2003
    ..Thus, FliH, though undoubtedly important, is not essential...
  20. pmc Crystallization and preliminary X-ray analysis of Salmonella FliI, the ATPase component of the type III flagellar protein-export apparatus
    Tohru Minamino
    Dynamic NanoMachine Project, ICORP, JST, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:973-5. 2006
    ..4 A resolution. Anomalous difference Patterson maps of Os-derivative and Pt-derivative crystals showed significant peaks in their Harker sections, indicating that both derivatives are suitable for structure determination...

Research Grants39

  1. SEQUENCE ANALYSIS OF THE BACTERIAL FLAGELLAR MOTOR
    ROBERT MACNAB; Fiscal Year: 1993
    ..These features will be mutated in a selective fashion, and the consequences for export and assembly monitored in an attempt to understand the recognition process...
  2. SEQUENCE ANALYSIS OF THE BACTERIAL FLAGELLAR MOTOR
    ROBERT MACNAB; Fiscal Year: 1992
    ..As hypotheses emerge from the mutant sequence analysis, they will be tested by generating site- directed mutations...
  3. PRINCIPLES OF SENSORY RECEPTION AND MOTOR FUNCTION
    ROBERT MACNAB; Fiscal Year: 1980
    ..Species under study are Salmonella, Escherichia coli and Bacillus subtilis. ..
  4. MOLECULAR ANALYSIS OF THE BACTERIAL FLAGELLAR MOTOR
    ROBERT MACNAB; Fiscal Year: 2001
    ....
  5. PRINCIPLES OF SENSORY RECEPTION AND MOTOR FUNCTION
    ROBERT MACNAB; Fiscal Year: 1993
    ..Physical interactions between different components will be studied by a gel overlay technique analogous to western blotting. An attempt will be made to determine the means by which the flagellar motor is anchored to the cell surface...
  6. PRINCIPLES OF SENSORY RECEPTION AND MOTOR FUNCTION
    ROBERT MACNAB; Fiscal Year: 2002
    ....