Haifan Lin

Summary

Affiliation: Yale University
Country: USA

Publications

  1. pmc Uniting germline and stem cells: the function of Piwi proteins and the piRNA pathway in diverse organisms
    Celina Juliano
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06509, USA
    Annu Rev Genet 45:447-69. 2011
  2. pmc A high-resolution whole-genome map of key chromatin modifications in the adult Drosophila melanogaster
    Hang Yin
    Yale Stem Cell Center and Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut, United States of America
    PLoS Genet 7:e1002380. 2011
  3. pmc Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation
    Vamsi K Gangaraju
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA
    Nat Genet 43:153-8. 2011
  4. pmc The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster
    Akos Szakmary
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    J Cell Biol 185:613-27. 2009
  5. pmc Capturing the Cloud: UAP56 in Nuage Assembly and Function
    Haifan Lin
    Yale Stem Cell Center and Departments of Cell Biology and Genetics, Yale University School of Medicine, New Haven, CT 06520, USA Electronic address
    Cell 151:699-701. 2012
  6. ncbi request reprint piRNAs in the germ line
    Haifan Lin
    Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 316:397. 2007
  7. pmc Cell biology of stem cells: an enigma of asymmetry and self-renewal
    Haifan Lin
    Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06511, USA
    J Cell Biol 180:257-60. 2008
  8. ncbi request reprint Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation
    Akos Szakmary
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 15:171-8. 2005
  9. ncbi request reprint The role of PIWI and the miRNA machinery in Drosophila germline determination
    Heather B Megosh
    Department of Cell Biology and Duke University Medical Center, Durham, North Carolina 27705, USA
    Curr Biol 16:1884-94. 2006
  10. ncbi request reprint An epigenetic activation role of Piwi and a Piwi-associated piRNA in Drosophila melanogaster
    Hang Yin
    Department of Cell Biology, Duke University Medical School, Durham, North Carolina 27710, USA
    Nature 450:304-8. 2007

Collaborators

Detail Information

Publications52

  1. pmc Uniting germline and stem cells: the function of Piwi proteins and the piRNA pathway in diverse organisms
    Celina Juliano
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06509, USA
    Annu Rev Genet 45:447-69. 2011
    ....
  2. pmc A high-resolution whole-genome map of key chromatin modifications in the adult Drosophila melanogaster
    Hang Yin
    Yale Stem Cell Center and Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut, United States of America
    PLoS Genet 7:e1002380. 2011
    ..These features, likely conserved among diverse epigenomes, reveal general strategies for chromatin modifications...
  3. pmc Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation
    Vamsi K Gangaraju
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA
    Nat Genet 43:153-8. 2011
    ..Moreover, they point to epigenetic silencing of the expression of existing genetic variants and the suppression of transposon-induced new genetic variation as two major mechanisms underlying piRNA pathway-mediated canalization...
  4. pmc The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster
    Akos Szakmary
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    J Cell Biol 185:613-27. 2009
    ..The entire Yb protein is necessary for piwi expression in niche cells. A double mutant of Yb and a novel locus show male germline loss, revealing a function for Yb in male germline stem cell maintenance...
  5. pmc Capturing the Cloud: UAP56 in Nuage Assembly and Function
    Haifan Lin
    Yale Stem Cell Center and Departments of Cell Biology and Genetics, Yale University School of Medicine, New Haven, CT 06520, USA Electronic address
    Cell 151:699-701. 2012
    ..The nuage is a hazy electron-dense structure unique to germ cells and is enriched in components of the piRNA pathway. Although the nuage is cytoplasmic, Zhang et al. now show that it is organized by an intranuclear protein, UAP56...
  6. ncbi request reprint piRNAs in the germ line
    Haifan Lin
    Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 316:397. 2007
    ..Recently, a class of small RNAs that interact with Piwi proteins has been discovered in the mammalian germ line and Drosophila. These Piwi-interacting RNAs (piRNAs) represent a distinct small RNA pathway...
  7. pmc Cell biology of stem cells: an enigma of asymmetry and self-renewal
    Haifan Lin
    Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06511, USA
    J Cell Biol 180:257-60. 2008
    ..Further investigations of these questions in diverse types of stem cells will significantly advance our knowledge of cell biology and allow us to effectively harness stem cells for therapeutic applications...
  8. ncbi request reprint Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation
    Akos Szakmary
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 15:171-8. 2005
    ....
  9. ncbi request reprint The role of PIWI and the miRNA machinery in Drosophila germline determination
    Heather B Megosh
    Department of Cell Biology and Duke University Medical Center, Durham, North Carolina 27705, USA
    Curr Biol 16:1884-94. 2006
    ..Beyond this, little is known about molecular activities underlying germline determination...
  10. ncbi request reprint An epigenetic activation role of Piwi and a Piwi-associated piRNA in Drosophila melanogaster
    Hang Yin
    Department of Cell Biology, Duke University Medical School, Durham, North Carolina 27710, USA
    Nature 450:304-8. 2007
    ..This activating function is probably achieved through interaction with at least 3R-TAS1 piRNA and is essential for germline stem-cell maintenance...
  11. pmc PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition
    Li Liu
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    Development 138:1863-73. 2011
    ..Overall, our study reveals a function of the nuage in safeguarding the germline genome against deleterious retrotransposition via the piRNA pathway...
  12. pmc Sex-lethal is a target of Bruno-mediated translational repression in promoting the differentiation of stem cell progeny during Drosophila oogenesis
    Zhong Wang
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Dev Biol 302:160-8. 2007
    ..Therefore, Sxl MS11 represents a novel target of Bruno-mediated translational repression required for cystoblast differentiation...
  13. pmc A Drosophila chromatin factor interacts with the Piwi-interacting RNA mechanism in niche cells to regulate germline stem cell self-renewal
    Tora K Smulders-Srinivasan
    Yale Stem Cell Center, Yale University, New Haven, CT 06509, USA
    Genetics 186:573-83. 2010
    ..These results reveal a novel epigenetic mechanism involving Corto and Piwi that defines the fate and signaling function of niche cells in maintaining GSCs...
  14. pmc A major epigenetic programming mechanism guided by piRNAs
    Xiao A Huang
    Yale Stem Cell Center and Department of Cell Biology, Yale School of Medicine, New Haven, CT 06519, USA
    Dev Cell 24:502-16. 2013
    ..Piwi deficiency drastically changed the epigenetic landscape and polymerase II profile throughout the genome, revealing the Piwi-piRNA mechanism as a major epigenetic programming mechanism in Drosophila...
  15. pmc Screens for piwi suppressors in Drosophila identify dosage-dependent regulators of germline stem cell division
    Tora K Smulders-Srinivasan
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genetics 165:1971-91. 2003
    ..The other two types are deficiencies that cause lethality and female-specific lethality in a piwi2 mutant background, revealing the zygotic function of piwi in somatic development...
  16. pmc MILI, a PIWI-interacting RNA-binding protein, is required for germ line stem cell self-renewal and appears to positively regulate translation
    Yingdee Unhavaithaya
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 284:6507-19. 2009
    ..These observations indicate that MILI may positively regulate translation and that such regulation is required for germ line stem cell self-renewal...
  17. pmc Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline
    Jonathan P Saxe
    Yale Stem Cell Center, Yale University, New Haven, CT 06519, USA
    EMBO J 32:1869-85. 2013
    ..These results shed light on mechanisms underlying primary piRNA biogenesis, an area in which information is conspicuously absent. ..
  18. pmc MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis
    Shane T Grivna
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:13415-20. 2006
    ..Together, our results indicate that MIWI, a PIWI subfamily protein, uses piRNA as the major, but not exclusive, binding partner, and it is associated with translational machinery...
  19. pmc PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells
    Celina E Juliano
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
    Proc Natl Acad Sci U S A 111:337-42. 2014
    ..This comprehensive analysis of the PIWI-piRNA pathway in the somatic stem/progenitor cells of a nonbilaterian animal suggests that this pathway originated with broader stem cell functionality. ..
  20. ncbi request reprint Nanos maintains germline stem cell self-renewal by preventing differentiation
    Zhong Wang
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Science 303:2016-9. 2004
    ..Thus, Nanos is essential for both establishing and maintaining GSCs by preventing their precocious entry into oogenesis. These functions are likely achieved by repressing the translation of differentiation factors in PGCs and GSCs...
  21. pmc The Yb body, a major site for Piwi-associated RNA biogenesis and a gateway for Piwi expression and transport to the nucleus in somatic cells
    Hongying Qi
    Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511, USA
    J Biol Chem 286:3789-97. 2011
    ..Finally, we discovered putative endo-siRNAs in the flamenco locus and the Yb dependence of their expression. These observations further implicate a role for Yb in transposon silencing via both the piRNA and endo-siRNA pathways...
  22. pmc Function of Piwi, a nuclear Piwi/Argonaute protein, is independent of its slicer activity
    Nicole Darricarrère
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    Proc Natl Acad Sci U S A 110:1297-302. 2013
    ..Instead, it is consistent with the alternative mode of Piwi function as a molecule involved in the piRNA-directed guidance of epigenetic factors to chromatin...
  23. pmc Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse
    Ergin Beyret
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Dev Biol 355:215-26. 2011
    ..These results pinpoint a function of the PIWI protein subfamily to meiosis during spermatogenesis...
  24. ncbi request reprint The stem-cell niche theory: lessons from flies
    Haifan Lin
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Rev Genet 3:931-40. 2002
    ..These findings are illuminating our understanding of the self-renewing mechanisms of tissue stem cells in general...
  25. ncbi request reprint Germline stem cells in the Drosophila ovary descend from pole cells in the anterior region of the embryonic gonad
    Miho Asaoka
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    Development 131:5079-89. 2004
    ....
  26. ncbi request reprint The division of Drosophila germline stem cells and their precursors requires a specific cyclin
    Zhong Wang
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 15:328-33. 2005
    ..Our results indicate that stem cells may use specific cell cycle regulators for their division...
  27. ncbi request reprint miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis
    Wei Deng
    Department of Cell Biology, Duke University Medical Center, P O Box 3709, DUMC, Durham, NC 27710, USA
    Dev Cell 2:819-30. 2002
    ..Whereas MIWI and CREM do not regulate each other's expression, MIWI complexes with mRNAs of ACT and CREM target genes. Hence, MIWI may control spermiogenesis by regulating the stability of these mRNAs...
  28. pmc A novel class of small RNAs in mouse spermatogenic cells
    Shane T Grivna
    Department of Cell Biology, Duke University, Durham, North Carolina 27710, USA
    Genes Dev 20:1709-14. 2006
    ..Furthermore, a subpopulation of piRNAs are associated with polysomes, suggesting their potential role in translational regulation...
  29. pmc MicroRNAs: key regulators of stem cells
    Vamsi K Gangaraju
    Yale Stem Cell Center, Yale University School of Medicine, 10 Amistad Street, New Haven, Connecticut 06520 8002, USA
    Nat Rev Mol Cell Biol 10:116-25. 2009
    ..Such a role has been shown in embryonic stem cells, germline stem cells and various somatic tissue stem cells. These findings reveal a new dimension of gene regulation in controlling stem cell fate and behaviour...
  30. pmc Essential roles of the chromatin remodeling factor BRG1 in spermatogenesis in mice
    Jianguan Wang
    Department of Cell Biology, Yale University, New Haven, Connecticut, USA
    Biol Reprod 86:186. 2012
    ..Our study reveals the essential role of Brg1 in meiosis and underscores the differences in the mechanisms of DNA repair between germ cells and somatic cells...
  31. doi request reprint PIWI proteins and PIWI-interacting RNAs in the soma
    Robert J Ross
    1 Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06509, USA 2
    Nature 505:353-9. 2014
    ....
  32. pmc Piwi genes are dispensable for normal hematopoiesis in mice
    Mona J Nolde
    Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut, United States of America Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, United States of America
    PLoS ONE 8:e71950. 2013
    ..Collectively, our data suggest that the function of the piwi gene subfamily is not required for normal adult hematopoiesis...
  33. pmc Mili interacts with tudor domain-containing protein 1 in regulating spermatogenesis
    Jianquan Wang
    Department of Cell Biology, Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06520, USA
    Curr Biol 19:640-4. 2009
    ..Our results suggest that Mili interacts with Tdrd1 in the nuage and chromatoid body. This interaction does not contribute to piRNA biogenesis but represents a regulatory mechanism that is critical for spermatogenesis...
  34. pmc Piecing together the mosaic of early mammalian development through microRNAs
    Adriana Blakaj
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    J Biol Chem 283:9505-8. 2008
    ..Systematic investigation of the role of miRNAs in ES cells and embryos will allow us to find missing pieces of the mosaic of early development...
  35. pmc piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism
    Ergin Beyret
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    Cell Res 22:1429-39. 2012
    ....
  36. pmc Beyond transposons: the epigenetic and somatic functions of the Piwi-piRNA mechanism
    Jamy C Peng
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, United States
    Curr Opin Cell Biol 25:190-4. 2013
    ..In addition, the function of this pathway in somatic cells has also been more systematically characterized. The new findings reveal the Piwi-piRNA pathway as a more general mechanism of gene regulation...
  37. pmc High-efficiency siRNA-based gene knockdown in human embryonic stem cells
    Yinghong Ma
    Yale Stem Cell Center, New Haven, Connecticut 06520, USA
    RNA 16:2564-9. 2010
    ....
  38. doi request reprint The microRNA regulation of stem cells
    Xiao Albert Huang
    Yale Stem Cell Center, Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA
    Wiley Interdiscip Rev Dev Biol 1:83-95. 2012
    ..The distinct expression profiles of miRNAs and their regulatory roles in various types of stem cells render these RNAs potentially effective tools for clinical diagnosis and therapy...
  39. doi request reprint Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells
    Shuping Peng
    Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut, USA
    Stem Cells 29:496-504. 2011
    ..We suggest that Lin28, working in concert with RHA, enhances the translation of genes important for the growth and survival of human embryonic stem cells...
  40. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011
    ..Our results indicate a conserved role for MITOPLD/Zuc in the piRNA pathway and link mitochondrial membrane metabolism/signaling to small RNA biogenesis...
  41. ncbi request reprint Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
    Dan Qiao
    Department of Cell Biology, Duke University Medical Center, PO Box 3709, DUMC, Durham, North Carolina, NC 27710, USA
    Oncogene 21:3988-99. 2002
    ..Thus, hiwi is specifically expressed in both normal and malignant spermatogenic cells in a maturation stage-dependent pattern, in which it might function in germ cell proliferation...
  42. pmc PIWI proteins are essential for early Drosophila embryogenesis
    Sneha Ramesh Mani
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    Dev Biol 385:340-9. 2014
    ..Our study demonstrates the essential function of PIWI proteins in the first phase of somatic development. ..
  43. doi request reprint High-efficiency transfection and siRNA-mediated gene knockdown in human pluripotent stem cells
    Yinghong Ma
    Yale Stem Cell Center, School of Medicine, Yale University, New Haven, Connecticut, USA
    Curr Protoc Stem Cell Biol . 2012
    ..Beyond reaching high transfection efficiency on pluripotent cells, this protocol should also have application to primary cells that are traditionally difficult to transfect...
  44. pmc Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis
    Dong Chen
    Yale Stem Cell Center and Department of Cell Biology, Yale School of Medicine, New Haven, CT 06519, USA
    Curr Biol 22:420-5. 2012
    ..These results indicate that key components of the p53 pathway are coordinately regulated by Pumilio 1 at the posttranscriptional level, which may exemplify an RNA operon...
  45. pmc Small noncoding RNAs in the germline
    Jonathan P Saxe
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06511, USA
    Cold Spring Harb Perspect Biol 3:a002717. 2011
    ..Investigation of the three small RNA pathways has started to reveal a new dimension of gene regulation with defining roles in germline specification and development...
  46. pmc The biogenesis and function of PIWI proteins and piRNAs: progress and prospect
    Travis Thomson
    Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut 06511, USA
    Annu Rev Cell Dev Biol 25:355-76. 2009
    ..These diverse functions may involve piRNAs and may be achieved via novel mechanisms of epigenetic and posttranscriptional regulation...
  47. pmc Repressing the repressor: a lincRNA as a MicroRNA sponge in embryonic stem cell self-renewal
    Ee Chun Cheng
    Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06509, USA
    Dev Cell 25:1-2. 2013
    ..In this issue of Developmental Cell, Wang et al. (2013) find that linc-RoR maintains human embryonic stem cell self-renewal by functioning as a sponge to trap miR-145, thus regulating core pluripotency factors Oct4, Nanog, and Sox2...
  48. ncbi request reprint Stem cells: to be and not to be
    Haifan Lin
    Nature 425:353-5. 2003
  49. pmc Drosophila PIWI associates with chromatin and interacts directly with HP1a
    Brent Brower-Toland
    Department of Biology, Washington University, Saint Louis, MO 63130, USA
    Genes Dev 21:2300-11. 2007
    ..These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome...
  50. pmc Dr. Haifan Lin. Interviewed by Han Lee and Rachel Rosenstein
    Haifan Lin
    Yale J Biol Med 79:187-91. 2006
    Dr. Haifan Lin is professor of Cell Biology at Yale University, where he studies the mechanism of stem cell self-renewal in fruit flies, mice, and human cancer cells. Recently named director of the Yale Stem Cell Center, Dr...
  51. ncbi request reprint Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
    Satomi Kuramochi-Miyagawa
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Shi, Osaka 565 0871, Japan
    Development 131:839-49. 2004
    ..These data indicate that MILI is essential for the differentiation of spermatocytes...
  52. ncbi request reprint Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells
    Noora Kotaja
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, B P 10142, 67404 Illkirch Strasbourg, France
    J Cell Sci 119:2819-25. 2006
    ..These results shed new light on the function of chromatoid bodies in the post-transcriptional regulation of gene expression in haploid germ cells...

Research Grants25

  1. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2006
    ..2. Define and characterize the functional domains of PIWI. 3. Identify PlWI-interacting proteins and characterize their epigenetic role in the stem/niche cell genomes. [unreadable] [unreadable] [unreadable]..
  2. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2007
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  3. Function of PIWI/Argonuate Proteins in Spermatogenesis
    Haifan Lin; Fiscal Year: 2007
    ..4) Identify Mili/Miwi-target miRNAs and determine their function in regulating mRNA. Because Piwi/Ago proteins have demonstrated roles in oncogenesis, the proposed studies should bear significant health implications. ..
  4. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2006
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  5. Function of PIWI/Argonuate Proteins in Spermatogenesis
    Haifan Lin; Fiscal Year: 2008
    ..4) Identify Mili/Miwi-target miRNAs and determine their function in regulating mRNA. Because Piwi/Ago proteins have demonstrated roles in oncogenesis, the proposed studies should bear significant health implications. ..
  6. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2008
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  7. Function of PIWI/Argonuate Proteins in Spermatogenesis
    Haifan Lin; Fiscal Year: 2009
    ..4) Identify Mili/Miwi-target miRNAs and determine their function in regulating mRNA. Because Piwi/Ago proteins have demonstrated roles in oncogenesis, the proposed studies should bear significant health implications. ..
  8. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2009
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  9. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2010
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  10. Function of PIWI/Argonuate Proteins in Spermatogenesis
    Haifan Lin; Fiscal Year: 2010
    ..4) Identify Mili/Miwi-target miRNAs and determine their function in regulating mRNA. Because Piwi/Ago proteins have demonstrated roles in oncogenesis, the proposed studies should bear significant health implications. ..
  11. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2006
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  12. Stem Cell Mechanism in the Murine Germline
    Haifan Lin; Fiscal Year: 2005
    ..4. Analyze the biochemical roles of MIWI and MILl in spermatogonial stem cell division. 5. Identify direct target genes of MIWI and MILl to further study MIWI/MILI-mediated mechanisms. ..
  13. Stem Cell Mechanism in the Murine Germline
    Haifan Lin; Fiscal Year: 2004
    ..4. Analyze the biochemical roles of MIWI and MILl in spermatogonial stem cell division. 5. Identify direct target genes of MIWI and MILl to further study MIWI/MILI-mediated mechanisms. ..
  14. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 1999
    ..The fourth specific aim is to analyze the molecular structure of these two genes and to determine the nature of their protein products. The final specific aim is to analyze the cellular behavior of the two proteins. ..
  15. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2000
    ..3. Analyze the biochemical role of YB in signaling cells. 4. Define regulatory relationship between genes involved in producing somatic signals. 5. Identify new genes interacting with piwi by genetic suppressor screens. ..
  16. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2001
    ..3. Analyze the biochemical role of YB in signaling cells. 4. Define regulatory relationship between genes involved in producing somatic signals. 5. Identify new genes interacting with piwi by genetic suppressor screens. ..
  17. Stem Cell Mechanism in the Murine Germline
    Haifan Lin; Fiscal Year: 2002
    ..4. Analyze the biochemical roles of MIWI and MILl in spermatogonial stem cell division. 5. Identify direct target genes of MIWI and MILl to further study MIWI/MILI-mediated mechanisms. ..
  18. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2002
    ..3. Analyze the biochemical role of YB in signaling cells. 4. Define regulatory relationship between genes involved in producing somatic signals. 5. Identify new genes interacting with piwi by genetic suppressor screens. ..
  19. Stem Cell Mechanism in the Murine Germline
    Haifan Lin; Fiscal Year: 2003
    ..4. Analyze the biochemical roles of MIWI and MILl in spermatogonial stem cell division. 5. Identify direct target genes of MIWI and MILl to further study MIWI/MILI-mediated mechanisms. ..
  20. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2003
    ..3. Analyze the biochemical role of YB in signaling cells. 4. Define regulatory relationship between genes involved in producing somatic signals. 5. Identify new genes interacting with piwi by genetic suppressor screens. ..
  21. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2004
    ..3. Analyze the biochemical role of YB in signaling cells. 4. Define regulatory relationship between genes involved in producing somatic signals. 5. Identify new genes interacting with piwi by genetic suppressor screens. ..
  22. Function of PIWI/Argonuate Proteins in Spermatogenesis
    Haifan Lin; Fiscal Year: 2010
    ..4) Identify Mili/Miwi-target miRNAs and determine their function in regulating mRNA. Because Piwi/Ago proteins have demonstrated roles in oncogenesis, the proposed studies should bear significant health implications. ..