Sin Hang Lee
Affiliation: Milford Hospital
Location: Milford , USA
MD anatomical and clinical pathologist
Lee S, Vigliotti V, Vigliotti J, Pappu S. Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories. Infect Agent Cancer. 2007;2:11 pubmed
..The DNA sequence is included in each report to the physician for comparison in following up patients with persistent HPV infection, a recognized tumor promoter in cancer induction. ..
Lee S, Vigliotti V, Pappu S. DNA sequencing validation of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acid tests. Am J Clin Pathol. 2008;129:852-9 pubmed publisher
..DNA sequencing is a useful tool in validating molecular diagnostics...
Lee S, Vigliotti V, Pappu S. HPV infection among women in a representative rural and suburban population of the USA. Int J Gynaecol Obstet. 2009;105:210-4 pubmed publisher
..DNA sequencing is a useful method for increasing the specificity of HPV genotyping as an aid to follow persistent high-risk HPV infections to reduce excessive colposcopies in populations with low cancer prevalence. ..
Lee S, Vigliotti V, Pappu S. Molecular tests for human papillomavirus (HPV), Chlamydia trachomatis and Neisseria gonorrhoeae in liquid-based cytology specimen. BMC Womens Health. 2009;9:8 pubmed publisher
..However, even a highly specific test for high-risk HPV genotyping may have unacceptably low positive predictive values for precancer lesion in populations with a low cervical cancer prevalence rate. ..
Lee S, Vigliotti V, Vigliotti J, Pappu S. Validation of human papillomavirus genotyping by signature DNA sequence analysis. BMC Clin Pathol. 2009;9:3 pubmed publisher
Lee S, Vigliotti V, Pappu S. Signature sequence validation of human papillomavirus type 16 (HPV-16) in clinical specimens. J Clin Pathol. 2010;63:235-9 pubmed publisher
..However, selection of the signature sequence for Basic Local Alignment Search Tool algorithms is crucial to distinguish certain HPV-16 variants from other closely related HPV genotypes. ..
Lee S, Vigliotti V, Vigliotti J, Jones W, Pappu S. Increased sensitivity and specificity of Borrelia burgdorferi 16S ribosomal DNA detection. Am J Clin Pathol. 2010;133:569-76 pubmed publisher
..This technology may be a valuable supplement to the current serologic tests for Lyme disease. ..
Lee S, Vigliotti V, Vigliotti J, Jones W, WILLIAMS J, Walshon J. Early Lyme disease with spirochetemia - diagnosed by DNA sequencing. BMC Res Notes. 2010;3:273 pubmed publisher
Lee S. Guidelines for the use of molecular tests for the detection and genotyping of human papilloma virus from clinical specimens. Methods Mol Biol. 2012;903:65-101 pubmed publisher