P Isenring

Summary

Affiliation: Yale University
Country: USA

Publications

  1. ncbi request reprint Comparison of Na-K-Cl cotransporters. NKCC1, NKCC2, and the HEK cell Na-L-Cl cotransporter
    P Isenring
    Cellular and Molecular Physiology Department, Yale University, New Haven, Connecticut 06510, USA
    J Biol Chem 273:11295-301. 1998
  2. pmc The role of transmembrane domain 2 in cation transport by the Na-K-Cl cotransporter
    P Isenring
    Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 95:7179-84. 1998
  3. ncbi request reprint Ion and bumetanide binding by the Na-K-Cl cotransporter. Importance of transmembrane domains
    P Isenring
    Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06510, USA
    J Biol Chem 272:24556-62. 1997
  4. pmc Mutagenic mapping of the Na-K-Cl cotransporter for domains involved in ion transport and bumetanide binding
    P Isenring
    Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06510, USA
    J Gen Physiol 112:549-58. 1998

Detail Information

Publications4

  1. ncbi request reprint Comparison of Na-K-Cl cotransporters. NKCC1, NKCC2, and the HEK cell Na-L-Cl cotransporter
    P Isenring
    Cellular and Molecular Physiology Department, Yale University, New Haven, Connecticut 06510, USA
    J Biol Chem 273:11295-301. 1998
    ..5-fold lower Na affinity, an 8-fold lower Rb affinity, and a 4-fold higher bumetanide affinity. These results suggest the presence of a novel isoform of NKCC in HEK-293 cells...
  2. pmc The role of transmembrane domain 2 in cation transport by the Na-K-Cl cotransporter
    P Isenring
    Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 95:7179-84. 1998
    ..Finally, unlike Cl, bumetanide binding was strongly affected by shark-human replacement of transmembrane domain 2, indicating that the bumetanide-binding site is not the same as the Cl-binding site...
  3. ncbi request reprint Ion and bumetanide binding by the Na-K-Cl cotransporter. Importance of transmembrane domains
    P Isenring
    Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06510, USA
    J Biol Chem 272:24556-62. 1997
    ..These results demonstrate that residues involved with ion translocation and inhibitor binding are within the large central domain that contains the 12 predicted transmembrane helices...
  4. pmc Mutagenic mapping of the Na-K-Cl cotransporter for domains involved in ion transport and bumetanide binding
    P Isenring
    Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06510, USA
    J Gen Physiol 112:549-58. 1998
    ..Unlike for the ions, changes in bumetanide affinity were nonlinear and difficult to interpret: the Ki(bumetanide) of a number of the constructs was outside the range of sNKCC1 and hNKCC1 Kis...