M Hochstrasser

Summary

Affiliation: Yale University
Country: USA

Publications

  1. ncbi request reprint Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase
    Min Deng
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, P O Box 208114, New Haven, Connecticut 06520 8114, USA
    Nature 443:827-31. 2006
  2. pmc A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance
    Alaron Lewis
    Departments of Cell Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 178:813-27. 2007
  3. pmc The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity
    Shyr Jiann Li
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Cell Biol 160:1069-81. 2003
  4. pmc Aberrant substrate engagement of the ER translocon triggers degradation by the Hrd1 ubiquitin ligase
    Eric M Rubenstein
    Deptartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Cell Biol 197:761-73. 2012
  5. pmc A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding
    Andrew R Kusmierczyk
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA
    Nat Struct Mol Biol 18:622-9. 2011
  6. pmc Disulfide engineering to map subunit interactions in the proteasome and other macromolecular complexes
    Mark Hochstrasser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
    Methods Mol Biol 832:349-62. 2012
  7. ncbi request reprint Modification of proteins by ubiquitin and ubiquitin-like proteins
    Oliver Kerscher
    Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Cell Dev Biol 22:159-80. 2006
  8. ncbi request reprint Lingering mysteries of ubiquitin-chain assembly
    Mark Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, New Haven, CT 06520, USA
    Cell 124:27-34. 2006
  9. ncbi request reprint Evolution and function of ubiquitin-like protein-conjugation systems
    M Hochstrasser
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520, USA
    Nat Cell Biol 2:E153-7. 2000
  10. pmc Origin and function of ubiquitin-like proteins
    Mark Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, PO Box 208114, New Haven, Connecticut 06520, USA
    Nature 458:422-9. 2009

Research Grants

  1. DEGRADTION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2001
  2. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2006
  3. PREDOCTORAL PROGRAM IN CELLULAR AND MOLECULAR BIOLOGY
    Mark Hochstrasser; Fiscal Year: 2007
  4. Function and Assembly of Eukaryotic Proteasome
    Mark Hochstrasser; Fiscal Year: 2009
  5. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2009
  6. Function and Assembly of Eukaryotic Proteasome
    Mark Hochstrasser; Fiscal Year: 2010
  7. DEGRADATION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2005
  8. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2005
  9. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2001
  10. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2003

Collaborators

Detail Information

Publications51

  1. ncbi request reprint Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase
    Min Deng
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, P O Box 208114, New Haven, Connecticut 06520 8114, USA
    Nature 443:827-31. 2006
    ..These data indicate that different membrane ubiquitin ligases are spatially sorted within the ER-nuclear envelope membrane system and that this differential localization contributes to their specificity...
  2. pmc A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance
    Alaron Lewis
    Departments of Cell Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 178:813-27. 2007
    ..The results raise the possibility that NPC-localized protein desumoylation may be a key regulatory event preventing inappropriate pre-mRNA export...
  3. pmc The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity
    Shyr Jiann Li
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Cell Biol 160:1069-81. 2003
    ..These data define key functional elements of Ulp1 and strongly suggest that subcellular localization is a physiologically significant constraint on SUMO isopeptidase specificity...
  4. pmc Aberrant substrate engagement of the ER translocon triggers degradation by the Hrd1 ubiquitin ligase
    Eric M Rubenstein
    Deptartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    J Cell Biol 197:761-73. 2012
    ..Hrd1 therefore likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the translocon...
  5. pmc A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding
    Andrew R Kusmierczyk
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA
    Nat Struct Mol Biol 18:622-9. 2011
    ....
  6. pmc Disulfide engineering to map subunit interactions in the proteasome and other macromolecular complexes
    Mark Hochstrasser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
    Methods Mol Biol 832:349-62. 2012
    ....
  7. ncbi request reprint Modification of proteins by ubiquitin and ubiquitin-like proteins
    Oliver Kerscher
    Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Annu Rev Cell Dev Biol 22:159-80. 2006
    ..The primary aim of this review is to summarize the latest developments in our understanding of the different Ubl-protein modification systems, including the shared and unique features of these related pathways...
  8. ncbi request reprint Lingering mysteries of ubiquitin-chain assembly
    Mark Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, New Haven, CT 06520, USA
    Cell 124:27-34. 2006
    ..Deciphering them will shed light on why substrates differ in the extent and timing of polyubiquitin modification and how ancillary ubiquitination factors function...
  9. ncbi request reprint Evolution and function of ubiquitin-like protein-conjugation systems
    M Hochstrasser
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520, USA
    Nat Cell Biol 2:E153-7. 2000
    ..Newly discovered parallels between the activation of ubiquitin and the biosynthesis of certain enzyme cofactors now hint at the possible evolutionary origins of the ubiquitin system...
  10. pmc Origin and function of ubiquitin-like proteins
    Mark Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, PO Box 208114, New Haven, Connecticut 06520, USA
    Nature 458:422-9. 2009
    ....
  11. ncbi request reprint Ubiquitin ligation without a ligase
    Mark Hochstrasser
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA
    Dev Cell 13:4-6. 2007
    ..An article in Molecular Cell (Hoeller et al., 2007) now describes the E3-independent monoubiquitination of certain proteins. The mechanism has interesting parallels to SUMO ligation...
  12. ncbi request reprint Biochemistry. All in the ubiquitin family
    M Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, New Haven, CT 06520, USA
    Science 289:563-4. 2000
    ..In a comprehensive Perspective, Hochstrasser brings us up to date with the many new members of the ubiquitin modifier family and their multitudinous and diverse protein targets...
  13. ncbi request reprint Molecular biology. New proteases in a ubiquitin stew
    Mark Hochstrasser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Science 298:549-52. 2002
  14. ncbi request reprint SP-RING for SUMO: new functions bloom for a ubiquitin-like protein
    M Hochstrasser
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, New Haven, CT 06520, USA
    Cell 107:5-8. 2001
    ..Three groups now describe related E3-like factors that enhance transfer of SUMO to specific proteins. This family of factors includes proteins important for chromosome condensation, signal transduction, and ion channel biogenesis...
  15. pmc Desumoylation of the endoplasmic reticulum membrane VAP family protein Scs2 by Ulp1 and SUMO regulation of the inositol synthesis pathway
    Rachael Felberbaum
    Departments of Molecular, Cell, and Developmental Biology, Yale University, New Haven, Connecticut, USA
    Mol Cell Biol 32:64-75. 2012
    ..These results provide the first evidence of cross-regulation between the SUMO and inositol pathways, including the sumoylation of an ER membrane protein central to phospholipid synthesis and phosphoinositide signaling...
  16. pmc The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways
    A Y Amerik
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Biol Cell 11:3365-80. 2000
    ....
  17. ncbi request reprint Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae
    A Y Amerik
    Yale University, Department of Molecular Biophysics and Biochemistry, New Haven, CT 06520, USA
    Biol Chem 381:981-92. 2000
    ..The data suggest that the DOA4 locus underwent a duplication after the divergence of K. lactis and S. cerevisiae. This information will facilitate fine-structure analysis of the Doa4 protein to help delineate its key functional elements...
  18. ncbi request reprint Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)
    Stefan G Kreft
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 281:4646-53. 2006
    ..Finally, we provide evidence that the likely human Doa10 ortholog, TEB4 (MARCH-VI), adopts a topology similar to that of Doa10...
  19. ncbi request reprint Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue
    Tommer Ravid
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    Nat Cell Biol 9:422-7. 2007
    ..The polyubiquitin chain can also be transferred to a lysine side chain, suggesting a mechanism for polyubiquitin chain assembly that precedes substrate modification...
  20. ncbi request reprint Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae
    J Thomas Hannich
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    J Biol Chem 280:4102-10. 2005
    ..The results point to a surprisingly broad array of cellular processes regulated by SUMO conjugation and provide a starting point for detailed studies of how SUMO ligation contributes to these different regulatory mechanisms...
  21. ncbi request reprint The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation
    Yang Xie
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    J Biol Chem 282:34176-84. 2007
    ..Our data reveal a novel mechanism of substrate targeting in which sumoylation of a protein can help trigger its subsequent ubiquitination by recruiting a SUMO-binding ubiquitin ligase...
  22. pmc Degradation of the bile salt export pump at endoplasmic reticulum in progressive familial intrahepatic cholestasis type II
    Lin Wang
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06510, USA
    Hepatology 48:1558-69. 2008
    ..Furthermore, we present evidence that G982R weakly associates with various components of the ER quality control system. These data together demonstrate that the PFIC II mutants except R1153C and DeltaGly are degraded by the ERAD pathway...
  23. doi request reprint Analysis of protein ubiquitination
    Jeffrey D Laney
    Yale University, New Haven, Connecticut, USA
    Curr Protoc Protein Sci . 2002
    ..These methods are suitable for a variety of eukaryotic cells, but techniques are specifically described for use with yeast and mammalian cells...
  24. pmc The short-lived Matalpha2 transcriptional repressor is protected from degradation in vivo by interactions with its corepressors Tup1 and Ssn6
    Jeffrey D Laney
    Department of Molecular Biophysics and Biochemistry, Brown University, Providence, RI 02912, USA
    Mol Cell Biol 26:371-80. 2006
    ..From these results, we propose that the fraction of alpha2 found in active regulatory complexes with Tup1 and Ssn6 is spared from rapid proteolytic destruction and is stabilized relative to the uncomplexed pool of the protein...
  25. pmc Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways
    Tommer Ravid
    Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA
    EMBO J 25:533-43. 2006
    ..Thus, while Doa10 ubiquitinates both membrane and soluble proteins, the mechanisms of subsequent proteasome targeting differ...
  26. pmc An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery
    Maria Francisca Arteaga
    Department of Cellular and Molecular Physiology, Yale University, New Haven, CT 06520 8026, USA
    Proc Natl Acad Sci U S A 103:11178-83. 2006
    ....
  27. pmc Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae
    Minoru Funakoshi
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Yeast 26:185-92. 2009
    ..The set of plasmids has been deposited in the non-profit plasmid repository Addgene (http://www.addgene.org)...
  28. ncbi request reprint A multimeric assembly factor controls the formation of alternative 20S proteasomes
    Andrew R Kusmierczyk
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, Connecticut 06520 8114, USA
    Nat Struct Mol Biol 15:237-44. 2008
    ..Our data demonstrate that Pba3-Pba4 orchestrates formation of a specific type of proteasome, the first example of a trans-acting factor that controls assembly of alternative proteasomal complexes...
  29. pmc Heterohexameric ring arrangement of the eukaryotic proteasomal ATPases: implications for proteasome structure and assembly
    Robert J Tomko
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    Mol Cell 38:393-403. 2010
    ..Finally, the ATPase ring structure casts light on alternative RP structural models and the mechanism of RP action...
  30. pmc Essential role of nuclear localization for yeast Ulp2 SUMO protease function
    Mary B Kroetz
    Department of Cell Biology, Yale University, New Haven, CT 06520 8114, USA
    Mol Biol Cell 20:2196-206. 2009
    ....
  31. pmc beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint
    Xia Li
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    EMBO J 26:2339-49. 2007
    ..We propose that dimerization is normally driven by the beta5 propeptide, an intramolecular chaperone, but beta7 addition overcomes an Ump1-dependent assembly checkpoint and stabilizes the precursor dimer...
  32. ncbi request reprint Assaying protein ubiquitination in Saccharomyces cerevisiae
    Jeffrey D Laney
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Methods Enzymol 351:248-57. 2002
  33. pmc The Ulp2 SUMO protease is required for cell division following termination of the DNA damage checkpoint
    David C Schwartz
    Department of Molecular Biophysics, Yale University, New Haven, CT 06520 8114, USA
    Mol Cell Biol 27:6948-61. 2007
    ....
  34. ncbi request reprint Mechanism and function of deubiquitinating enzymes
    Alexander Y Amerik
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, PO Box 208114, New Haven, CT 06520 8114, USA
    Biochim Biophys Acta 1695:189-207. 2004
    ..We also discuss briefly some of the proteases that act on ubiquitin-like protein (UBL) conjugates and compare them to DUBs...
  35. pmc Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base
    Minoru Funakoshi
    Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520 8114, USA
    Cell 137:887-99. 2009
    ..Our results demonstrate that proteasomal RP biogenesis requires multiple, functionally overlapping chaperones and suggest a model in which subunits form specific subcomplexes that then assemble into the base...
  36. pmc SUMO-independent in vivo activity of a SUMO-targeted ubiquitin ligase toward a short-lived transcription factor
    Yang Xie
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Genes Dev 24:893-903. 2010
    ..We suggest that alpha2, and presumably other proteins, have surface features that mimic SUMO, and therefore can directly recruit STUbLs without prior SUMO conjugation...
  37. pmc Plasticity in eucaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast
    Irina Velichutina
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA
    EMBO J 23:500-10. 2004
    ..Importantly, biogenesis of proteasomes is delayed at an early stage in pre9Delta cells, suggesting an advantage for Pre9 over Pre6 incorporation at the alpha3 position that facilitates correct assembly...
  38. ncbi request reprint Ulp2 and the DNA damage response: desumoylation enables safe passage through mitosis
    Rachael Felberbaum
    Department of Molecular, Cell, and Developmental Biology, Yale University, New Haven, Connecticut, USA
    Cell Cycle 7:52-6. 2008
    ....
  39. pmc Ubiquitin-dependent degradation of the yeast Mat(alpha)2 repressor enables a switch in developmental state
    Jeffrey D Laney
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520 8114, USA
    Genes Dev 17:2259-70. 2003
    ..Analogous epigenetic mechanisms for reprogramming transcription are likely to operate in many developmental pathways...
  40. ncbi request reprint NF-kappaB signaling: flipping the switch with polyubiquitin chains
    Tommer Ravid
    Yale University, Department of Molecular Biophysics and Biochemistry, 266 Whitney Avenue, P O Box 208114 New Haven, Connecticut 06520 8114, USA
    Curr Biol 14:R898-900. 2004
    ..Protein modification by ubiquitin has emerged as an important cellular regulatory mechanism. Recent studies illustrate the surprising ways in which polyubiquitin chains are manipulated in the regulation of NF-kappaB signaling...
  41. pmc Identification of SUMO-interacting proteins by yeast two-hybrid analysis
    Mary B Kroetz
    Department of Cell Biology, Yale University, New Haven, CT, USA
    Methods Mol Biol 497:107-20. 2009
    ..Sumoylation can be verified by independent methods, such as a SUMO mobility shift assay. Finally, the chapter will compare the two-hybrid approach with mass spectrometric analysis as a means of identifying SUMO-interacting proteins...
  42. pmc Some assembly required: dedicated chaperones in eukaryotic proteasome biogenesis
    Andrew R Kusmierczyk
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
    Biol Chem 389:1143-51. 2008
    ....
  43. ncbi request reprint Preparation and characterization of yeast and human desumoylating enzymes
    Shyr Jiann Li
    Celera, South San Francisco, CA 94020, USA
    Methods Enzymol 398:457-67. 2005
  44. pmc A conserved late endosome-targeting signal required for Doa4 deubiquitylating enzyme function
    Alexander Amerik
    Department of Pharmacology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Cell Biol 175:825-35. 2006
    ..This is the first demonstration of a physiologically important DUB subcellular localization signal and provides a striking example of the functional diversification of DUB paralogues by the evolution of alternative spatial signals...
  45. ncbi request reprint Ubiquitin signalling: what's in a chain?
    Mark Hochstrasser
    Nat Cell Biol 6:571-2. 2004
  46. ncbi request reprint Sem1, the yeast ortholog of a human BRCA2-binding protein, is a component of the proteasome regulatory particle that enhances proteasome stability
    Minoru Funakoshi
    Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    J Cell Sci 117:6447-54. 2004
    ..Our data suggest a potential mechanism for this protein-protein stabilization and also suggest that an intact proteasomal regulatory particle is required for responses to DNA damage...
  47. pmc Diversity of degradation signals in the ubiquitin-proteasome system
    Tommer Ravid
    Department of Biological Chemistry, Hebrew University of Jerusalem, Jerusalem, Israel
    Nat Rev Mol Cell Biol 9:679-90. 2008
    ....
  48. ncbi request reprint Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein
    Gregory Huyer
    Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:38369-78. 2004
    ....
  49. ncbi request reprint Varshavsky's contributions
    Wolfgang Baumeister
    Science 306:1290-2. 2004
  50. pmc Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing
    Shengbing Huang
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 102:13410-5. 2005
    ..Furthermore, Rtt106p interacts with CAF-1 physically through Cac1p. These biochemical and genetic data indicate that Rtt106p is a previously uncharacterized histone chaperone connecting S phase to epigenetic inheritance...
  51. ncbi request reprint Ubiquitin-dependent control of development in Saccharomyces cerevisiae
    Jeffrey D Laney
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Box G J2, Providence, Rhode Island 02912, USA
    Curr Opin Microbiol 7:647-54. 2004
    ....

Research Grants24

  1. DEGRADTION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2001
    ..Aim 4. Continue structure-function studies on the yeast 20S proteasome and assess its interactions with the l9S regulatory complex. ..
  2. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2006
    ..4. Determine the molecular basis for the differences in specificity and activiity between the Ulpl and U|p2 desumoylating enzymes. ..
  3. PREDOCTORAL PROGRAM IN CELLULAR AND MOLECULAR BIOLOGY
    Mark Hochstrasser; Fiscal Year: 2007
    ..D. training. Teaching experience is an essential part of training and all students teach with supervision and guidance from the faculty. The average time to graduation is 6 years. ..
  4. Function and Assembly of Eukaryotic Proteasome
    Mark Hochstrasser; Fiscal Year: 2009
    ..A potential RP assembly factor recently discovered in the PI's laboratory provides the starting point for these studies. ..
  5. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2009
    ..This project aims to deepen our understanding of the enzymes that detach certain ubiquitin-like proteins from their partners, with the long-term goal of developing therapies to treat patients suffering from cancer and other diseases. ..
  6. Function and Assembly of Eukaryotic Proteasome
    Mark Hochstrasser; Fiscal Year: 2010
    ..A potential RP assembly factor recently discovered in the PI's laboratory provides the starting point for these studies. ..
  7. DEGRADATION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2005
    ..abstract_text> ..
  8. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2005
    ..4. Determine the molecular basis for the differences in specificity and activiity between the Ulpl and U|p2 desumoylating enzymes. ..
  9. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2001
    ....
  10. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2003
    ....
  11. DEGRADTION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2004
    ..Aim 4. Continue structure-function studies on the yeast 20S proteasome and assess its interactions with the l9S regulatory complex. ..
  12. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2002
    ....
  13. DEGRADTION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2002
    ..Aim 4. Continue structure-function studies on the yeast 20S proteasome and assess its interactions with the l9S regulatory complex. ..
  14. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2000
    ....
  15. DEGRADATION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2006
    ..abstract_text> ..
  16. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2007
    ..4. Determine the molecular basis for the differences in specificity and activiity between the Ulpl and U|p2 desumoylating enzymes. ..
  17. DEGRADTION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2003
    ..Aim 4. Continue structure-function studies on the yeast 20S proteasome and assess its interactions with the l9S regulatory complex. ..
  18. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2004
    ..4. Determine the molecular basis for the differences in specificity and activiity between the Ulpl and U|p2 desumoylating enzymes. ..
  19. DEGRADATION OF SHORT-LIVED REGULATORY PROTEINS IN YEAST
    Mark Hochstrasser; Fiscal Year: 2007
    ..abstract_text> ..
  20. FUNCTIONS AND MECHANISMS OF DEUBIQUITINATING ENZYMES
    Mark Hochstrasser; Fiscal Year: 2010
    ..This project aims to deepen our understanding of the enzymes that detach certain ubiquitin-like proteins from their partners, with the long-term goal of developing therapies to treat patients suffering from cancer and other diseases. ..