A Helenius

Summary

Affiliation: Yale University
Country: USA

Publications

  1. ncbi Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit
    E S Trombetta
    Department of Cell Biology, Yale University School of Medicine, P O Box 208002, New Haven, Connecticut 06520 8002, USA
    J Biol Chem 271:27509-16. 1996
  2. pmc Transient, lectin-like association of calreticulin with folding intermediates of cellular and viral glycoproteins
    J R Peterson
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Biol Cell 6:1173-84. 1995
  3. pmc The number and location of glycans on influenza hemagglutinin determine folding and association with calnexin and calreticulin
    D N Hebert
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 139:613-23. 1997
  4. ncbi In vitro reconstitution of calreticulin-substrate interactions
    J R Peterson
    Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA
    J Cell Sci 112:2775-84. 1999
  5. pmc Misfolding and aggregation of newly synthesized proteins in the endoplasmic reticulum
    T Marquardt
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510
    J Cell Biol 117:505-13. 1992
  6. pmc Calnexin and calreticulin promote folding, delay oligomerization and suppress degradation of influenza hemagglutinin in microsomes
    D N Hebert
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 8002, USA
    EMBO J 15:2961-8. 1996
  7. ncbi Lectins as chaperones in glycoprotein folding
    E S Trombetta
    Department of Cell Biology, Yale Medical School, New Haven, CT 06520 8002, USA
    Curr Opin Struct Biol 8:587-92. 1998
  8. pmc Quality control in the secretory pathway: the role of calreticulin, calnexin and BiP in the retention of glycoproteins with C-terminal truncations
    J X Zhang
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510 8002, USA
    Mol Biol Cell 8:1943-54. 1997
  9. pmc Conformational requirements for glycoprotein reglucosylation in the endoplasmic reticulum
    E S Trombetta
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 148:1123-29. 2000
  10. pmc Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p
    J F Simons
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 8002, USA
    EMBO J 17:396-405. 1998

Collaborators

Detail Information

Publications18

  1. ncbi Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit
    E S Trombetta
    Department of Cell Biology, Yale University School of Medicine, P O Box 208002, New Haven, Connecticut 06520 8002, USA
    J Biol Chem 271:27509-16. 1996
    ..It encoded a soluble protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. This suggested that the beta subunit is responsible for the ER localization of the enzyme...
  2. pmc Transient, lectin-like association of calreticulin with folding intermediates of cellular and viral glycoproteins
    J R Peterson
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Biol Cell 6:1173-84. 1995
    ..Both are therefore likely to play an important role in glycoprotein maturation and quality control in the endoplasmic reticulum...
  3. pmc The number and location of glycans on influenza hemagglutinin determine folding and association with calnexin and calreticulin
    D N Hebert
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 139:613-23. 1997
    ..In summary, the data established that individual N-linked glycans in HA have distinct roles in calnexin/calreticulin binding and in co- and posttranslational folding...
  4. ncbi In vitro reconstitution of calreticulin-substrate interactions
    J R Peterson
    Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA
    J Cell Sci 112:2775-84. 1999
    ..We demonstrated, moreover, that the acidic C-terminal 62 residues of calreticulin are dispensable for substrate binding whereas further deletions inhibit substrate binding...
  5. pmc Misfolding and aggregation of newly synthesized proteins in the endoplasmic reticulum
    T Marquardt
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510
    J Cell Biol 117:505-13. 1992
    ..BiP was associated noncovalently with the aggregates and with some of the nonaggregated products. We conclude that aggregation reflects the poor solubility of incompletely folded polypeptide chains...
  6. pmc Calnexin and calreticulin promote folding, delay oligomerization and suppress degradation of influenza hemagglutinin in microsomes
    D N Hebert
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 8002, USA
    EMBO J 15:2961-8. 1996
    ..Taken together, these results indicated that CNX and CRT promote correct folding by inhibiting aggregation, preventing premature oxidation and oligomerization, and by suppressing degradation of incompletely folded glycopolypeptides...
  7. ncbi Lectins as chaperones in glycoprotein folding
    E S Trombetta
    Department of Cell Biology, Yale Medical School, New Haven, CT 06520 8002, USA
    Curr Opin Struct Biol 8:587-92. 1998
    ....
  8. pmc Quality control in the secretory pathway: the role of calreticulin, calnexin and BiP in the retention of glycoproteins with C-terminal truncations
    J X Zhang
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510 8002, USA
    Mol Biol Cell 8:1943-54. 1997
    ..In summary, the results showed that the quality control system in the secretory pathway was efficient and sensitive to folding defects, and that it involved multiple interactions with endoplasmic reticulum chaperones...
  9. pmc Conformational requirements for glycoprotein reglucosylation in the endoplasmic reticulum
    E S Trombetta
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 148:1123-29. 2000
    ..The findings suggest that discrete populations of nonnative conformations are selectively reglucosylated to participate in the calnexin/calreticulin chaperone pathway...
  10. pmc Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p
    J F Simons
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 8002, USA
    EMBO J 17:396-405. 1998
    ..We concluded that glucose trimming in S.cerevisiae is necessary for proper cell wall synthesis, and that the glucosidases function synergistically with BiP/Kar2p in this process...
  11. ncbi Quaternary and domain structure of glycoprotein processing glucosidase II
    E S Trombetta
    Department of Cell Biology, Yale University School of Medicine, P O Box 208002, New Haven, Connecticut 06520 8002, USA
    Biochemistry 40:10717-22. 2001
    ..Through its C-terminal HDEL signal, the beta subunit may retain the complete alpha(1)beta(1) complex in the ER...
  12. pmc Glycoprotein reglucosylation and nucleotide sugar utilization in the secretory pathway: identification of a nucleoside diphosphatase in the endoplasmic reticulum
    E S Trombetta
    Department of Cell Biology, Yale Medical School, PO Box 208002, New Haven, CT 06520 8002, USA
    EMBO J 18:3282-92. 1999
    ..By eliminating UDP, which is an inhibitory product of the UDP-Glc:glycoprotein glucosyltransferase, it is likely to promote reglucosylation reactions involved in glycoprotein folding and quality control in the ER...
  13. pmc Interactions between newly synthesized glycoproteins, calnexin and a network of resident chaperones in the endoplasmic reticulum
    U Tatu
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    J Cell Biol 136:555-65. 1997
    ....
  14. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  15. pmc Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins
    C L Howe
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510
    Proc Natl Acad Sci U S A 85:7577-81. 1988
    ....
  16. ncbi Interaction of newly synthesized apolipoprotein B with calnexin and calreticulin requires glucose trimming in the endoplasmic reticulum
    U Tatu
    Department of Biochemistry, Indian Institute of Science, Bangalore, India
    Biosci Rep 19:189-96. 1999
    ..The results showed that like for association with other glycoprotein substrates, trimming of glucose residues was essential for ApoB binding to CN and CR...
  17. ncbi Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells
    B L Granger
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510
    J Biol Chem 265:12036-43. 1990
    ..Mouse lgp110 is encoded by at least seven exons; intron positions suggest that the two homologous ectodomains of each lgp arose through gene duplication...
  18. ncbi Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER
    L Pelkmans
    Institute of Biochemistry, Swiss Federal Institute of Technology, Universitaetstrasse 16, , Switzerland
    Nat Cell Biol 3:473-83. 2001
    ..This pathway bypasses endosomes and the Golgi complex, and is part of the productive infectious route used by SV40...