Mark Chernyak

Summary

Affiliation: Yale University
Country: USA

Publications

  1. pmc Infectious particles, stress, and induced prion amyloids: a unifying perspective
    Laura Manuelidis
    Yale University Medical School, New Haven, CT, USA
    Virulence 4:373-83. 2013
  2. pmc Transmissible encephalopathy agents: virulence, geography and clockwork
    Laura Manuelidis
    Yale University, Section of Neuropathology, Surgery, New Haven, CT, USA
    Virulence 1:101-4. 2010
  3. doi request reprint Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD
    Laura Manuelidis
    Yale University Medical School, 333 Cedar Street, New Haven, CT 06510, USA
    J Neurovirol 17:131-45. 2011
  4. pmc The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt-Jakob disease and scrapie agents
    Laura Manuelidis
    Section of Neuropathology, Yale University Medical School, 333 Cedar Street, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 106:13529-34. 2009
  5. pmc Strain-specific viral properties of variant Creutzfeldt-Jakob disease (vCJD) are encoded by the agent and not by host prion protein
    Laura Manuelidis
    Yale Medical School, 333 Cedar Street, New Haven, Connecticut 06510, USA
    J Cell Biochem 106:220-31. 2009
  6. pmc Cells infected with scrapie and Creutzfeldt-Jakob disease agents produce intracellular 25-nm virus-like particles
    Laura Manuelidis
    Yale Medical School, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 104:1965-70. 2007
  7. ncbi request reprint A 25 nm virion is the likely cause of transmissible spongiform encephalopathies
    Laura Manuelidis
    Yale Medical School, New Haven, CT 06510, USA
    J Cell Biochem 100:897-915. 2007
  8. ncbi request reprint A virus behind the mask of prions?
    Laura Manuelidis
    Yale Medical School, New Haven, CT 06510, USA
    Folia Neuropathol 42:10-23. 2004
  9. ncbi request reprint Evolution of a strain of CJD that induces BSE-like plaques
    L Manuelidis
    Section of Neuropathology, Yale Medical School, 310 Cedar Street, New Haven, CT 06510, USA
    Science 277:94-8. 1997
  10. pmc Vaccination with an attenuated Creutzfeldt-Jakob disease strain prevents expression of a virulent agent
    L Manuelidis
    Section of Neuropathology, Yale Medical School, 310 Cedar Street, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 95:2520-5. 1998

Research Grants

  1. Experimental Creutzfeldt Jacob Disease
    Laura Manuelidis; Fiscal Year: 2007
  2. EXPERIMENTAL CREUTZFELDT-JAKOB DISEASE
    Laura Manuelidis; Fiscal Year: 1993
  3. EXPERIMENTAL CREUTZFELDT-JAKOB DISEASE
    Laura Manuelidis; Fiscal Year: 1999
  4. Experimental Creutzfeldt Jacob Disease
    Laura Manuelidis; Fiscal Year: 2010

Collaborators

  • Ying Liu
  • R A Flavell
  • Kohtaro Miyazawa
  • Laura Manuelidis
  • Christopher A Baker
  • L Manuelidis
  • Zhi Yun Lu
  • Brian Mullins
  • Ru Sun
  • Noriuki Nishida
  • Alvaro Arjona
  • K Radebold
  • Trisha Chakrabarty
  • Kaitlin Emmerling
  • Nana Aba Nduom
  • He Zhang
  • Nuria Barquero
  • W Fritch
  • Zhoa Xue Yu
  • Nuria Banquero
  • Shigeru Katamine
  • Noriyuki Nishida
  • Laura Simarro
  • Florian Islinger
  • Daniel Martin
  • D Martin
  • Z Y Lu
  • Z Yun Lu
  • P Koni
  • I Zaitsev
  • Y G Xi

Detail Information

Publications24

  1. pmc Infectious particles, stress, and induced prion amyloids: a unifying perspective
    Laura Manuelidis
    Yale University Medical School, New Haven, CT, USA
    Virulence 4:373-83. 2013
    ..This innate process however, once initiated, can become unstoppable in accelerated neuronal aging. ..
  2. pmc Transmissible encephalopathy agents: virulence, geography and clockwork
    Laura Manuelidis
    Yale University, Section of Neuropathology, Surgery, New Haven, CT, USA
    Virulence 1:101-4. 2010
    ..The virus-like biology of unique TSE agents, including epidemic spread, mutation, and superinfection, can be used to systematically define features of virulence that distinguish common endemic from newly emerging strains...
  3. doi request reprint Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD
    Laura Manuelidis
    Yale University Medical School, 333 Cedar Street, New Haven, CT 06510, USA
    J Neurovirol 17:131-45. 2011
    ..The current results also raise the intriguing possibility that mammals may incorporate more of the prokaryotic world in their cytoplasm than previously recognized...
  4. pmc The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt-Jakob disease and scrapie agents
    Laura Manuelidis
    Section of Neuropathology, Yale University Medical School, 333 Cedar Street, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 106:13529-34. 2009
    ..The geographic independence of the kuru agent provides additional reasons to explore causal environmental pathogens in these infectious neurodegenerative diseases...
  5. pmc Strain-specific viral properties of variant Creutzfeldt-Jakob disease (vCJD) are encoded by the agent and not by host prion protein
    Laura Manuelidis
    Yale Medical School, 333 Cedar Street, New Haven, Connecticut 06510, USA
    J Cell Biochem 106:220-31. 2009
    ..Monotypic cultures can be used to identify the intrinsic, strain-determining molecules of TSE infectious particles...
  6. pmc Cells infected with scrapie and Creutzfeldt-Jakob disease agents produce intracellular 25-nm virus-like particles
    Laura Manuelidis
    Yale Medical School, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 104:1965-70. 2007
    ....
  7. ncbi request reprint A 25 nm virion is the likely cause of transmissible spongiform encephalopathies
    Laura Manuelidis
    Yale Medical School, New Haven, CT 06510, USA
    J Cell Biochem 100:897-915. 2007
    ..These models provide new ways to rapidly identify intrinsic viral and strain-specific molecules so important for diagnosis, prevention, and fundamental understanding...
  8. ncbi request reprint A virus behind the mask of prions?
    Laura Manuelidis
    Yale Medical School, New Haven, CT 06510, USA
    Folia Neuropathol 42:10-23. 2004
    ..Recently developed TSE tissue culture models offer a way of identifying rapidly the intrinsic and strain-specific agent molecules so needed for diagnosis and prevention...
  9. ncbi request reprint Evolution of a strain of CJD that induces BSE-like plaques
    L Manuelidis
    Section of Neuropathology, Yale Medical School, 310 Cedar Street, New Haven, CT 06510, USA
    Science 277:94-8. 1997
    ..Early vacuolar change involving activated microglia and astrocytes preceded significant PrP-res accumulation by more than 50 days. These studies reveal several inflammatory host reactions to an exogenous agent...
  10. pmc Vaccination with an attenuated Creutzfeldt-Jakob disease strain prevents expression of a virulent agent
    L Manuelidis
    Section of Neuropathology, Yale Medical School, 310 Cedar Street, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 95:2520-5. 1998
    ....
  11. pmc Follicular dendritic cells and dissemination of Creutzfeldt-Jakob disease
    L Manuelidis
    Section of Neuropathology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Virol 74:8614-22. 2000
    ..While it is still not clear where the infectious agent replicates, macrophages can sequester appreciable levels of infectivity and hence act as reservoirs for prolonged latent infection...
  12. ncbi request reprint Transmissible encephalopathies: speculations and realities
    Laura Manuelidis
    Yale Medical School, New Haven, Connecticut 06510, USA
    Viral Immunol 16:123-39. 2003
    ..Moreover, the epidemic spread of TSEs, and the activation of host innate immune mechanisms by infection, further indicate these agents are recognizably foreign, and probably viral...
  13. pmc Virus-like interference in the latency and prevention of Creutzfeldt-Jakob disease
    Laura Manuelidis
    Yale University Medical School, Farnum Memorial Basement 11, 333 Cedar Street, New Haven CT 06510, USA
    Proc Natl Acad Sci U S A 100:5360-5. 2003
    ..In principle, it should be possible to artificially stimulate relevant myeloid pathways to better prevent and/or delay the clinical and pathological sequelae of these infections...
  14. ncbi request reprint Attenuated Creutzfeldt-Jakob Disease agents can hide more virulent infections
    L Manuelidis
    Section of Neuropathology, Yale Medical School 310 Cedar Street, New Haen, CT 06510, New Haven, USA
    Neurosci Lett 293:163-6. 2000
    ..This powerful model of agent-induced repression also implicates targets other than prion protein (PrP) in eliminating infection...
  15. pmc Blood borne transit of CJD from brain to gut at early stages of infection
    K Radebold
    Section of Neuropathology, Yale Medical School, 310 Cedar St, New Haven, CT 06510, USA
    BMC Infect Dis 1:20. 2001
    ..In Creutzfeldt-Jakob disease (CJD) and other related transmissible spongiform encephalopathies it is critical to understand the various pathways by which the infectious agent spreads to different organs...
  16. ncbi request reprint Reciprocal interference between specific CJD and scrapie agents in neural cell cultures
    Noriuki Nishida
    Yale Medical School, New Haven, CT 06510, USA
    Science 310:493-6. 2005
    ..This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread...
  17. pmc A rapid accurate culture assay for infectivity in Transmissible Encephalopathies
    Ying Liu
    Section of Neuropathology, Yale Medical School, New Haven, CT 06510, USA
    J Neurovirol 14:352-61. 2008
    ..Standardized GT1 assays can be used for direct comparison of different agent strains, and will facilitate the rapid isolation of essential agent components...
  18. pmc Agent-specific Shadoo responses in transmissible encephalopathies
    Kohtaro Miyazawa
    Yale University Medical School, 333 Cedar Street, New Haven, CT 06510, USA
    J Neuroimmune Pharmacol 5:155-63. 2010
    ..Furthermore, because all infected GT1 cells appeared as healthy as uninfected controls, Sho was not needed to protect infected cells from their "toxic" burden of abundant abnormal PrP and intracellular amyloid...
  19. pmc Quantitative recovery of scrapie agent with minimal protein from highly infectious cultures
    Ru Sun
    Section of Neuropathology, Yale Medical School, New Haven, Connecticut 06510, USA
    Viral Immunol 21:293-302. 2008
    ..Because no foreign enzymes were added to achieve reasonably purified infectious particles, these preparations may be used to elicit diagnostic antibodies to foreign agent proteins...
  20. pmc Unique inflammatory RNA profiles of microglia in Creutzfeldt-Jakob disease
    Christopher A Baker
    Section of Neuropathology, Yale University School of Medicine, 333 Cedar Street, FMB 11, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 100:675-9. 2003
    ..Transcript profiles unique for CJD microglia and other myeloid cells provide opportunities for more sensitive preclinical diagnoses of infectious and noninfectious neurodegenerative diseases...
  21. ncbi request reprint New molecular markers of early and progressive CJD brain infection
    Zhi Yun Lu
    Yale Medical School, New Haven, Connecticut 06510, USA
    J Cell Biochem 93:644-52. 2004
    ..The many host responses to the CJD agent challenge the assumption that the immune system does not recognize TSE infections because these agents are composed only of the host's own PrP...
  22. pmc Two Creutzfeldt-Jakob disease agents reproduce prion protein-independent identities in cell cultures
    Alvaro Arjona
    Yale Medical School, 333 Cedar Street, Farnum Memorial Basement 11, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 101:8768-73. 2004
    ..The continuous substantial replication of CJD in monotypic cells will further the discrimination of agent-specific molecules from pathological host responses to infection...
  23. ncbi request reprint Early induction of interferon-responsive mRNAs in Creutzfeldt-Jakob disease
    Christopher A Baker
    Section of Neuropathology, Yale School of Medicine, New Haven, Connecticut, USA
    J Neurovirol 10:29-40. 2004
    ..These data suggest the CJD agent is recognized as a foreign virus-like entity. Moreover, the early reactive gene expression profiles described here may be useful in preclinical diagnosis...
  24. pmc Microglia from Creutzfeldt-Jakob disease-infected brains are infectious and show specific mRNA activation profiles
    Christopher A Baker
    Section of Neuropathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Virol 76:10905-13. 2002
    ..Because microglia develop a specific pattern of responses to the CJD agent, microglial markers may be exploited in the diagnosis of these spongiform encephalopathies...

Research Grants17

  1. Experimental Creutzfeldt Jacob Disease
    Laura Manuelidis; Fiscal Year: 2007
    ..Rapid infectivity assays in vitro can open the study of TSEs by providing a way to test any pure molecule or structure for its intrinsic infectivity by Koch's established postulates for infectious pathogens. ..
  2. EXPERIMENTAL CREUTZFELDT-JAKOB DISEASE
    Laura Manuelidis; Fiscal Year: 1993
    ..We will also investigate minor protein species using 2-D electrophoresis and other methods with high sensitivity in order to identify CJD-specific proteins that are recovered under several experimental conditions...
  3. EXPERIMENTAL CREUTZFELDT-JAKOB DISEASE
    Laura Manuelidis; Fiscal Year: 1999
    ..These are currently being analyzed. If the method is as robust as it appears to be, it can be applied for the discovery of other unsuspected or new RNA viruses that participate in late-onset dementias. ..
  4. Experimental Creutzfeldt Jacob Disease
    Laura Manuelidis; Fiscal Year: 2010
    ..Rapid infectivity assays can also benefit the design of drugs to ambush the progressive replication of these agents before irreversible neurodegeneration begins. ..