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Genomes and GenesSpecies | W F BoronSummaryAffiliation: Yale University Country: USA Publications
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Regulation of intracellular pHWalter F Boron
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8026, USA
Adv Physiol Educ 28:160-79. 2004....- Acid-base transport by the renal proximal tubuleWalter F Boron
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8026, USA
J Am Soc Nephrol 17:2368-82. 2006..Together, these processes ensure that the proximal tubule responds appropriately to acute acid-base disturbances and thereby contributes to the regulation of blood pH... - Sodium-coupled bicarbonate transportersW F Boron
Department of Cell and Molecular Physiology, Yale University School of Medicine New Haven, CT 06520 8026, USA
JOP 2:176-81. 2001..A characteristic of most, but not all, BT's is that they are inhibited rather effectively by 4,4'-diisothiocyanostilbene-4,4'-disulfonate (DIDS)... - Expression cloning and characterization of a renal electrogenic Na+/HCO3- cotransporterM F Romero
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
Nature 387:409-13. 1997.... - The electrogenic Na/HCO3 cotransporterW F Boron
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA
Wien Klin Wochenschr 109:445-56. 1997..Using aNBC to screen a rat-kidney cDNA library, we identified a full-length cDNA clone (rNBC), rNBC encodes a protein of 1035 amino acids, is 86% identical to aNBC, and can be functionally expressed in oocytes... - Sodium-hydrogen exchangers and sodium-bicarbonate co-transporters: ontogeny of protein expression in the rat brainR M Douglas
Department of Pediatrics, Section of Respiratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
Neuroscience 102:217-28. 2001..These data indicate that the expression of sodium-hydrogen exchanger and sodium-bicarbonate co-transporter proteins varies as a function of both development and specific brain region... 
Characterization of human SLC4A10 as an electroneutral Na/HCO3 cotransporter (NBCn2) with Cl- self-exchange activityMark D Parker
Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06520, USA
J Biol Chem 283:12777-88. 2008..We propose to rename NCBE as the second electroneutral Na/HCO(3) cotransporter, NBCn2...- An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channelI Choi
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Nature 405:571-5. 2000..Oocytes injected with low levels of NBCn1-B complementary RNA exhibit a Na+ conductance that 4,4-diisothiocyanatostilbene-2,2'-disulphonate stimulates slowly and irreversibly... - Immunolocalization of the electrogenic Na+-HCO-3 cotransporter in mammalian and amphibian kidneyB M Schmitt
Department of Cellular and Molecular Physiology, Section of Nephrology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Am J Physiol 276:F27-38. 1999.... - Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchangerI I Grichtchenko
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 276:8358-63. 2001..Running in reverse, NDCBE1 requires extracellular Cl(-). Thus, NDCBE1 encodes a human, electroneutral Na(+)-driven Cl-HCO3 exchanger... - Expression and localization of Na-driven Cl-HCO(3)(-) exchanger (SLC4A8) in rodent CNSL M Chen
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
Neuroscience 153:162-74. 2008..Thus, NDCBE is in a position to contribute substantially to pH(i) regulation in multiple CNS neurons... - Cloning of a Na+-driven Cl/HCO3 exchanger from squid giant fiber lobeLeila V Virkki
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Am J Physiol Cell Physiol 285:C771-80. 2003..Transport was blocked by 50 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). The characteristics of our novel clone fit those of a Na+-driven Cl/HCO3 exchanger (NDCBE)... - Cloning and characterization of a human electrogenic Na+-HCO-3 cotransporter isoform (hhNBC)I Choi
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Am J Physiol 276:C576-84. 1999..We conclude that hhNBC is an electrogenic Na+-HCO-3 cotransporter and that hkNBC is also electrogenic... - Cloning and functional expression of an MIP (AQP0) homolog from killifish (Fundulus heteroclitus) lensL V Virkki
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, SHM B 133, PO Box 208026, New Haven, CT 06520 8026, USA
Am J Physiol Regul Integr Comp Physiol 281:R1994-2003. 2001..The mercurials HgCl(2) and p-chloromercuribenzenesulfonate inhibit the water permeability of MIPfun by approximately 25%. MIPfun is not permeable to glycerol, urea, or formic acid but is weakly permeable to CO(2)... - Cloning and characterization of novel human SLC4A8 gene products encoding Na+-driven Cl-/HCO3(-) exchanger variants NDCBE-A, -C, and -DMark D Parker
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, OH 44106, USA
Physiol Genomics 34:265-76. 2008.... - Effect of expressing the water channel aquaporin-1 on the CO2 permeability of Xenopus oocytesN L Nakhoul
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Am J Physiol 274:C543-8. 1998..Even if AQP1 should mediate a CO2 flux, it would remain to be determined whether this CO2 movement is quantitatively important... - Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobePeter M Piermarini
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA
Am J Physiol Cell Physiol 292:C2032-45. 2007..Extracellular Cl(-) is not necessary for the transporter to operate. Therefore, SF3 is an NBCe, representing the first NBCe characterized from an invertebrate... - Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAGRaif Musa-Aziz
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 106:5406-11. 2009..Thus, each channel exhibits a characteristic ratio for indices of CO(2) vs. NH(3) permeability, demonstrating that, like ion channels, gas channels can exhibit selectivity... - Use of a new polyclonal antibody to study the distribution and glycosylation of the sodium-coupled bicarbonate transporter NCBE in rodent brainL M Chen
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Neuroscience 151:374-85. 2008..In oocytes, mutating any one of the three consensus N-glycosylation sites reduces glycosylation of the other two, and the triple mutant exhibits negligible functional expression... - Effects of angiotensin II on the CO2 dependence of HCO3- reabsorption by the rabbit S2 renal proximal tubuleYuehan Zhou
Dept. of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Am J Physiol Renal Physiol 290:F666-73. 2006.... - Evidence against a direct interaction between intracellular carbonic anhydrase II and pure C-terminal domains of SLC4 bicarbonate transportersPeter M Piermarini
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 282:1409-21. 2007..Thus, although an HCO3 metabolon may exist, CA II cannot bind directly to pure SLC4-Ct peptides and can bind to GST-SLC4-Ct fusion proteins only when the CA II is immobilized and the fusion protein is soluble, and not vice versa... - Effect of isolated removal of either basolateral HCO-3 or basolateral CO2 on HCO-3 reabsorption by rabbit S2 proximal tubuleJinhua Zhao
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
Am J Physiol Renal Physiol 285:F359-69. 2003.... - Expression of Na+/H+ and HCO3- -dependent transporters in Na+/H+ exchanger isoform 1 null mutant mouse brainJ Xue
Department of Pediatrics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Neuroscience 122:37-46. 2003..The alteration is region-specific. An increase in acid extruders (e.g. NHE3) and a decrease in acid loaders (e.g. AE3) suggest that there are some compensatory mechanisms that occur in NHE1 null mutant mice... - Sodium kinetics of Na,K-ATPase alpha isoforms in intact transfected HeLa cellsR Zahler
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
J Gen Physiol 110:201-13. 1997..We conclude that the apparent Km(Na+) for rat Na-K pump isoforms increases in the sequence alpha1 < alpha2 < alpha3. The alpha3 isoform may be suited for handling large Na+ loads in electrically active cells... - Inhibition of K/HCO(3) cotransport in squid axons by quaternary ammonium ionsB A Davis
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
J Membr Biol 183:25-32. 2001..The identification of inhibitors of the K/HCO(3) cotransporter, for which no inhibitors previously existed, will facilitate the study of this transporter... - Colony-stimulating factor-1 increases osteoclast intracellular pH and promotes survival via the electroneutral Na/HCO3 cotransporter NBCn1Patrice Bouyer
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, POB 208026, New Haven, Connecticut 06520 8026, USA
Endocrinology 148:831-40. 2007..This study provides the first evidence that osteoclasts express a CSF-1-regulated Na/HCO(3) cotransporter, which may play a role in cell survival... - Reversible and irreversible interactions of DIDS with the human electrogenic Na/HCO3 cotransporter NBCe1-A: role of lysines in the KKMIK motif of TM5Jing Lu
Department Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar St, New Haven, CT 06520 8026, USA
Am J Physiol Cell Physiol 292:C1787-98. 2007..The fitted maximal inhibitions were 80-91% for the first five constructs, and 66% for NNMIN. Thus DIDS probably reversibly binds before irreversibly reacting with NBCe1-A. Finally, tenidap blocks not only KKMIK, but also NNMIN and EEMIE... - A conductive pathway generated from fragments of the human red cell anion exchanger AE1Mark D Parker
Department of Biochemistry, University of Bristol, University Walk, Bristol, BS8 1TD, UK
J Physiol 581:33-50. 2007..Although this conductive pathway is not a usual feature of intact mammalian AE1, it shares many properties with the anion-conductive pathways intrinsic to two other Cl-HCO3- exchangers, trout AE1 and mammalian SLC26A7... - Role of glycosylation in the renal electrogenic Na+-HCO3- cotransporter (NBCe1)Inyeong Choi
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Am J Physiol Renal Physiol 284:F1199-206. 2003..The triple mutant (N592Q/N597Q/N617Q) is completely unglycosylated but, based on microelectrode measurements of membrane potential and pH(i) in oocytes, preserves the Na(+) and HCO(3)(-) dependence and electrogenicity of wild-type NBCe1... - Role of endogenously secreted angiotensin II in the CO2-induced stimulation of HCO3 reabsorption by renal proximal tubulesYuehan Zhou
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA
Am J Physiol Renal Physiol 294:F245-52. 2008..We conclude that the isolated PT predominantly secretes preformed ANG II, rather than angiotensinogen or ANG I... - Effects of acute hypoxia on intracellular-pH regulation in astrocytes cultured from rat hippocampusMark O Bevensee
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
Brain Res 1193:143-52. 2008.... - Expression and purification of the cytoplasmic N-terminal domain of the Na/HCO3 cotransporter NBCe1-A: structural insights from a generalized approachHarindarpal S Gill
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Protein Expr Purif 49:228-34. 2006..A comparison of Nt-NBCe1 to SLC4 member Nt-AE1, based on purification properties and predicted secondary-structure sequence alignments, suggests a similar mechanism for dimer stabilization... - ATP dependence of Na+-driven Cl-HCO3 exchange in squid axonsBruce A Davis
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
J Membr Biol 222:107-13. 2008..0 pmol cm(-2 )s(-1)) but increased modestly by 1 mM ADP beta S (J (H) = 16.0 pmol cm(-2 )s(-1)). We suggest that ATP gamma S leads to stable phosphorylation of the transporter or an essential activator... - Functional characterization of human NBC4 as an electrogenic Na+-HCO cotransporter (NBCe2)Leila V Virkki
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520 8026, USA
Am J Physiol Cell Physiol 282:C1278-89. 2002..This observation, as well as the absolute E(rev) values, are consistent with a 2:1 stoichiometry. In conclusion, the behavior of NBC4c, which we propose to call NBCe2-c, is similar to that of NBCe1, the first electrogenic NBC... - Role of the AT1A receptor in the CO2-induced stimulation of HCO3- reabsorption by renal proximal tubulesYuehan Zhou
Dept of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar St, New Haven, CT 06520, USA
Am J Physiol Renal Physiol 293:F110-20. 2007..Neither apical AT(2) receptors nor basolateral ANG II receptors are involved in these processes... - Cloning and functional characterization of a novel aquaporin from Xenopus laevis oocytesLeila V Virkki
Department of Cellular and Molecular Physiology, Yale University School of Medicine, PO Box 108026, 333 Cedar Street, New Haven, CT 06520, USA
J Biol Chem 277:40610-6. 2002..Remarkably, low HgCl(2) concentrations (0.3-10 microm) reduced P(f) and urea uptake, whereas high concentrations (300-1000 microm) reversed the inhibition. We propose that AQPxlo is a new AQP paralogue unknown in mammals... - An increase in intracellular calcium concentration that is induced by basolateral CO2 in rabbit renal proximal tubulePatrice Bouyer
Department of Cellular and Molecular Physiology, Yale University, School of Medicine, 333 Cedar St, PO Box 208026, New Haven, CT 06520 8026, USA
Am J Physiol Renal Physiol 285:F674-87. 2003..Adding 10 mM caffeine (ryanodine-receptor agonist) had no effect on [Ca2+]i. Thus basolateral CO2, presumably via a basolateral sensor, triggers the release of Ca2+ from a nonconventional intracellular pool... - Effect of extracellular acid-base disturbances on the intracellular pH of neurones cultured from rat medullary raphe or hippocampusPatrice Bouyer
Department of Cellular and Molecular Physiology, SHM B 133, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
J Physiol 559:85-101. 2004..Moreover, regardless of whether neurones were cultured from the chemosensitive or the non-chemosensitive region, pH(i) did not recover during any of the acid-base stresses... - Evidence from renal proximal tubules that HCO3- and solute reabsorption are acutely regulated not by pH but by basolateral HCO3- and CO2Yuehan Zhou
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 102:3875-80. 2005..Because the cells cannot respond acutely to pH changes, we propose that the responses of J(HCO(3)) and the reabsorption of other solutes to changes in [HCO(3)(-)](BL) or [CO(2)](BL) involve sensors for basolateral HCO(3)(-) and CO(2)... - Effect of human carbonic anhydrase II on the activity of the human electrogenic Na/HCO3 cotransporter NBCe1-A in Xenopus oocytesJing Lu
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 281:19241-50. 2006..Thus, fused CA II was functional. Slope conductances of EGFP-e1-CAII versus EGFP-e1 oocytes were indistinguishable, and EZA had no effect. Thus, even when fused to NBCe1-A, CA II does not enhance NBCe1-A activity... - Preliminary X-ray diffraction analysis of the cytoplasmic N-terminal domain of the Na/HCO3 cotransporter NBCe1-AHarindarpal S Gill
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:534-7. 2006..9 A3 Da(-1), with two monomers of molecular weight approximately 83 kDa in the asymmetric unit. The molecular- replacement packing solution shows that the molecules form dimers by a domain-swapping mechanism... - Role of a tyrosine kinase in the CO2-induced stimulation of HCO3- reabsorption by rabbit S2 proximal tubulesYuehan Zhou
Dept. of Cellular and Molecular Physiology, Yale Univ. School of Medicine, 333 Cedar St, New Haven, CT 06520, USA
Am J Physiol Renal Physiol 291:F358-67. 2006.... - Concentration-dependent effects on intracellular and surface pH of exposing Xenopus oocytes to solutions containing NH3/NH4(+)Raif Musa-Aziz
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
J Membr Biol 228:15-31. 2009.... - Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidneyM F Romero
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Am J Physiol 274:F425-32. 1998..Subsequent removal of Na+ in the presence of CO2/HCO3- causes a depolarization of > 50 mV and a concomitant decrease of pHi. Thus rNBC is in the same newly identified family of Na(+)-linked HCO3- transporters as is aNBC... - Arginine vasopressin enhances pHi regulation in the presence of HCO3- by stimulating three acid-base transport systemsM B Ganz
Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06511
Nature 337:648-51. 1989..The stimulation of three acid-base transporters by the growth factor AVP greatly enhances the ability of the cell to regulate pHi... - Specificity of anion exchange mediated by mouse Slc26a6Zhirong Jiang
Department of Internal Medicine, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8029, USA
J Biol Chem 277:33963-7. 2002..In the presence of high oxalate concentrations as found in renal tubular fluid and urine, Slc26a6 may largely function as an electrogenic Cl(-)-oxalate exchanger... - Chronic continuous hypoxia decreases the expression of SLC4A7 (NBCn1) and SLC4A10 (NCBE) in mouse brainLi Ming Chen
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA
Am J Physiol Regul Integr Comp Physiol 293:R2412-20. 2007..We hypothesize that these decreases, which are out of proportion to the expected overall decreases in brain protein levels, may especially be important for reducing energy consumption... - Transport of volatile solutes through AQP1Gordon J Cooper
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520-8026, USA
J Physiol 542:17-29. 2002.... - The electrogenicity of the rat sodium-bicarbonate cotransporter NBCe1 requires interactions among transmembrane segments of the transporterInyeong Choi
Department of Physiology, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322, USA
J Physiol 578:131-42. 2007..Thus, these chimeras were electroneutral, as were two others consisting of NBCe1(Nt-L)/NBCn1(TM6-Ct) and NBCn1(Nt-L)/NBCe1(TM6-Ct). We propose that the electrogenicity of NBCe1 requires interactions between TM1-5 and TM6-13... - Na+-dependent HCO3- uptake into the rat choroid plexus epithelium is partially DIDS sensitiveElena V Bouzinova
Institute for Physiology and Biophysics, Univ of Aarhus, Ole Worms Alle, Bldg 1160, DK 8000 Aarhus C, Denmark
Am J Physiol Cell Physiol 289:C1448-56. 2005..This is consistent with the localization of the three base transporters NBCn1, Na+-driven Cl- bicarbonate exchanger, and NBCe2 in this tissue... - The SLC4 family of HCO 3 - transportersMichael F Romero
Departments of Physiology and Biophysics and Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106 4970, USA
Pflugers Arch 447:495-509. 2004..SLC4 gene products play important roles in the carriage of CO(2) by erythrocytes, the absorption or secretion of H(+) or HCO(3)(-) by several epithelia, as well as the regulation of cell volume and intracellular pH... - The human NBCe1-A mutant R881C, associated with proximal renal tubular acidosis, retains function but is mistargeted in polarized renal epitheliaAshley M Toye
Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
Am J Physiol Cell Physiol 291:C788-801. 2006..In summary, the pathophysiology of pRTA caused by the R881C mutation is likely due to a deficit of NBCe1-A at the proximal tubule basolateral membrane, rather than a defect in the transport activity of individual molecules... - Physiology and the Olympics ... on our fourth anniversaryWalter F Boron
Physiology (Bethesda) 23:182. 2008 - Effect of chronic elevated carbon dioxide on the expression of acid-base transporters in the neonatal and adult mouseAmjad Kanaan
Department of Pediatrics, Section of Respiratory Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Am J Physiol Regul Integr Comp Physiol 293:R1294-302. 2007.... - Exploring gas permeability of cellular membranes and membrane channels with molecular dynamicsYi Wang
Theoretical and Computational Biophysics Group, Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
J Struct Biol 157:534-44. 2007..As such, gas conduction through AQP1 may only be physiologically relevant either in membranes of low gas permeability, or in cells where a major fraction of the cellular membrane is occupied by AQPs...
Research Grants
- Physiology of Electrogenic Na/HCO3 CotransportersWalter Boron; Fiscal Year: 2007..The results could have important implications for understanding the normal control of acid-base balance and renal-tubule acidosis. ..