K L Behar

Summary

Affiliation: Yale University
Country: USA

Publications

  1. ncbi request reprint In vivo nuclear magnetic resonance studies of glutamate-gamma-aminobutyric acid-glutamine cycling in rodent and human cortex: the central role of glutamine
    K L Behar
    Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06520, USA
    J Nutr 131:2498S-504S; discussion 2523S-4S. 2001
  2. ncbi request reprint Preliminary evidence of low cortical GABA levels in localized 1H-MR spectra of alcohol-dependent and hepatic encephalopathy patients
    K L Behar
    Department of Neurology, Yale University, New Haven, CT 06520 8043, USA
    Am J Psychiatry 156:952-4. 1999
  3. ncbi request reprint In vivo (13)C NMR measurement of neurotransmitter glutamate cycling, anaplerosis and TCA cycle flux in rat brain during
    N R Sibson
    Departments of Molecular Biophysics, Yale University School of Medicine, New Haven, Connecticut, USA
    J Neurochem 76:975-89. 2001
  4. pmc In vivo 13C NMR measurements of cerebral glutamine synthesis as evidence for glutamate-glutamine cycling
    N R Sibson
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520 8043, USA
    Proc Natl Acad Sci U S A 94:2699-704. 1997
  5. pmc Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity
    N R Sibson
    Department of Molecular Biophysics, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:316-21. 1998
  6. ncbi request reprint Functional energy metabolism: in vivo 13C-NMR spectroscopy evidence for coupling of cerebral glucose consumption and glutamatergic neuronalactivity
    N R Sibson
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, Newhaven, Conn, USA
    Dev Neurosci 20:321-30. 1998
  7. ncbi request reprint Decrease in GABA synthesis rate in rat cortex following GABA-transaminase inhibition correlates with the decrease in GAD(67) protein
    G F Mason
    Department of Psychiatry, Magnetic Resonance Center for Research in Metabolism and Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Brain Res 914:81-91. 2001
  8. ncbi request reprint Glutamine is the major precursor for GABA synthesis in rat neocortex in vivo following acute GABA-transaminase inhibition
    A B Patel
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Brain Res 919:207-20. 2001
  9. ncbi request reprint Reduced cortical gamma-aminobutyric acid levels in depressed patients determined by proton magnetic resonance spectroscopy
    G Sanacora
    Department of Psychiatry, Yale University School of Medicine, New Haven, Conn, USA
    Arch Gen Psychiatry 56:1043-7. 1999
  10. pmc Glial pathology in an animal model of depression: reversal of stress-induced cellular, metabolic and behavioral deficits by the glutamate-modulating drug riluzole
    M Banasr
    Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06437, USA
    Mol Psychiatry 15:501-11. 2010

Detail Information

Publications47

  1. ncbi request reprint In vivo nuclear magnetic resonance studies of glutamate-gamma-aminobutyric acid-glutamine cycling in rodent and human cortex: the central role of glutamine
    K L Behar
    Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06520, USA
    J Nutr 131:2498S-504S; discussion 2523S-4S. 2001
    ..Thus, glutamine appears to play a central role in the normal functional energetics of the cerebral cortex...
  2. ncbi request reprint Preliminary evidence of low cortical GABA levels in localized 1H-MR spectra of alcohol-dependent and hepatic encephalopathy patients
    K L Behar
    Department of Neurology, Yale University, New Haven, CT 06520 8043, USA
    Am J Psychiatry 156:952-4. 1999
    ..The aim of the study was to compare levels of neuroactive amino acids in the cerebral cortex of healthy subjects, recently detoxified alcohol-dependent patients, and patients with hepatic encephalopathy...
  3. ncbi request reprint In vivo (13)C NMR measurement of neurotransmitter glutamate cycling, anaplerosis and TCA cycle flux in rat brain during
    N R Sibson
    Departments of Molecular Biophysics, Yale University School of Medicine, New Haven, Connecticut, USA
    J Neurochem 76:975-89. 2001
    ..These findings indicate that anaplerotic glutamine synthesis is coupled to nitrogen removal from the brain (ammonia detoxification) under hyperammonemic conditions...
  4. pmc In vivo 13C NMR measurements of cerebral glutamine synthesis as evidence for glutamate-glutamine cycling
    N R Sibson
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520 8043, USA
    Proc Natl Acad Sci U S A 94:2699-704. 1997
    ....
  5. pmc Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity
    N R Sibson
    Department of Molecular Biophysics, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:316-21. 1998
    ..Another implication of this result is that brain activation studies, which map cortical oxidative Glc metabolism, provide a quantitative measure of synaptic glutamate release...
  6. ncbi request reprint Functional energy metabolism: in vivo 13C-NMR spectroscopy evidence for coupling of cerebral glucose consumption and glutamatergic neuronalactivity
    N R Sibson
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, Newhaven, Conn, USA
    Dev Neurosci 20:321-30. 1998
    ..These findings bear upon a number of hypotheses concerning the neurophysiological basis of brain functional imaging methods...
  7. ncbi request reprint Decrease in GABA synthesis rate in rat cortex following GABA-transaminase inhibition correlates with the decrease in GAD(67) protein
    G F Mason
    Department of Psychiatry, Magnetic Resonance Center for Research in Metabolism and Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Brain Res 914:81-91. 2001
    ..GAD(67) could account for 56-85% of cortical GABA synthesis flux under basal conditions and the entire flux after vigabatrin treatment...
  8. ncbi request reprint Glutamine is the major precursor for GABA synthesis in rat neocortex in vivo following acute GABA-transaminase inhibition
    A B Patel
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA
    Brain Res 919:207-20. 2001
    ..07 micromol/g) based on the average precursor enrichments between 1 and 3 h. We conclude that glutamine is the major source of GABA carbon in the rat neocortex produced acutely following GABA-T inhibition by gabaculine in vivo...
  9. ncbi request reprint Reduced cortical gamma-aminobutyric acid levels in depressed patients determined by proton magnetic resonance spectroscopy
    G Sanacora
    Department of Psychiatry, Yale University School of Medicine, New Haven, Conn, USA
    Arch Gen Psychiatry 56:1043-7. 1999
    ..In this study we used in vivo proton magnetic resonance spectroscopy to investigate the hypothesis that abnormalities in the GABA neurotransmitter system are associated with the neurobiologic processes of depression...
  10. pmc Glial pathology in an animal model of depression: reversal of stress-induced cellular, metabolic and behavioral deficits by the glutamate-modulating drug riluzole
    M Banasr
    Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06437, USA
    Mol Psychiatry 15:501-11. 2010
    ....
  11. ncbi request reprint Reductions in occipital cortex GABA levels in panic disorder detected with 1h-magnetic resonance spectroscopy
    A W Goddard
    Yale Anxiety Clinic, Yale Department of Psychiatry, 100 York St, Room 2J, New Haven, CT 06511, USA
    Arch Gen Psychiatry 58:556-61. 2001
    ..The purpose of the present work was to determine whether cortical GABA levels are abnormally low in patients with panic disorder...
  12. ncbi request reprint Quantitative functional imaging of the brain: towards mapping neuronal activity by BOLD fMRI
    F Hyder
    Department of Diagnostic Radiology, Magnetic Resonance Center for Research in Metabolism and Physiology, Yale University, New Haven, CT 06510, USA
    NMR Biomed 14:413-31. 2001
    ..Consequences of these results from rat brain for similar calibrated BOLD fMRI studies in the human brain are discussed...
  13. ncbi request reprint Altered cerebral glucose and acetate metabolism in succinic semialdehyde dehydrogenase-deficient mice: evidence for glial dysfunction and reduced glutamate/glutamine cycling
    G M I Chowdhury
    Department of Diagnostic Radiology, Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, Connecticut, USA
    J Neurochem 103:2077-91. 2007
    ..Our findings indicate that SSADH deficiency is associated with major alterations in glutamate and glutamine metabolism in glia and neurons with surprisingly lesser effects on GABA synthesis...
  14. ncbi request reprint Differential increase in cerebral cortical glucose oxidative metabolism during rat postnatal development is greater in vivo than in vitro
    E J Novotny
    Department of Neurology, Yale University School of Medicine, New Haven, CT 06520, USA
    Brain Res 888:193-202. 2001
    ....
  15. ncbi request reprint Changes in N-acetylaspartate and myo-inositol detected in the cerebral cortex of hamsters with Creutzfeldt-Jakob disease
    K L Behar
    Department of Neurology, Yale University School of Medicine, New Haven, CT, USA
    Magn Reson Imaging 16:963-8. 1998
    ..Because they also correspond to the changes found in other dementias, including Alzheimer's disease and HIV dementia, these changes indicate converging pathogenetic pathways involved in many neurodegenerative diseases...
  16. ncbi request reprint Inhibition of voltage-dependent sodium channels suppresses the functional magnetic resonance imaging response to forepaw somatosensory activation in the rodent
    I Kida
    Department of Diagnostic Radiology, Yale University, New Haven, Connecticut 06510, USA
    J Cereb Blood Flow Metab 21:585-91. 2001
    ..These results strongly suggest that activation of voltage-dependent Na+ channels is involved in the BOLD fMRI responses during somatosensory activation of the rat cortex...
  17. ncbi request reprint Glutamatergic neurotransmission and neuronal glucose oxidation are coupled during intense neuronal activation
    Anant B Patel
    Department of Psychiatry, Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Cereb Blood Flow Metab 24:972-85. 2004
    ..These results indicate that neuronal glucose oxidation and not total glucose utilization is coupled to the glutamate/glutamine cycle during intense cortical activation...
  18. ncbi request reprint Cerebral pyruvate carboxylase flux is unaltered during bicuculline-seizures
    Anant B Patel
    Department of Diagnostic Radiology and Magnetic Resonance Research Center, Yale University School of Medicine, PO Box 208043, 300 Cedar Street, New Haven, CT 06520, USA
    J Neurosci Res 79:128-38. 2005
    ..2004b)...
  19. ncbi request reprint Impaired GABA neuronal response to acute benzodiazepine administration in panic disorder
    Andrew W Goddard
    Indiana University Department of Psychiatry, Adult Psychiatry Clinic, UH 3124A, 550 North University Blvd, Indianapolis, IN 46202 5266, USA
    Am J Psychiatry 161:2186-93. 2004
    ..In the current study, the authors tested the hypothesis that panic disorder patients have a deficient GABA neuronal response to benzodiazepine (clonazepam) administration...
  20. pmc The contribution of GABA to glutamate/glutamine cycling and energy metabolism in the rat cortex in vivo
    Anant B Patel
    Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 102:5588-93. 2005
    ..The contribution of GABAergic neurons and inhibition to cortical energy metabolism has broad implications for the interpretation of functional imaging signals...
  21. ncbi request reprint Lamotrigine suppresses neurophysiological responses to somatosensory stimulation in the rodent
    Ikuhiro Kida
    Magnetic Resonance Research Center MRRC, Yale University, New Haven, CT 06510, USA
    Neuroimage 29:216-24. 2006
    ....
  22. ncbi request reprint Acute regulation of steady-state GABA levels following GABA-transaminase inhibition in rat cerebral cortex
    Robin A de Graaf
    Department of Diagnostic Radiology, Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, Connecticut 06520 8043, USA
    Neurochem Int 48:508-14. 2006
    ..The findings suggest that astroglia may be the site of continuing GABA catabolism after acute vigabatrin treatment...
  23. ncbi request reprint Evidence that GAD65 mediates increased GABA synthesis during intense neuronal activity in vivo
    Anant B Patel
    Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA
    J Neurochem 97:385-96. 2006
    ..3-fold increase in inorganic phosphate, known to be a potent activator of apoGAD(65)in vitro. Our results are consistent with a major role for GAD(65) in activity-dependent GABA synthesis...
  24. ncbi request reprint High magnetic field water and metabolite proton T1 and T2 relaxation in rat brain in vivo
    Robin A de Graaf
    Magnetic Resonance Research Center, Yale University, School of Medicine, New Haven, Connecticut 06520 8043, USA
    Magn Reson Med 56:386-94. 2006
    ....
  25. ncbi request reprint Regional whole body fat quantification in mice
    Xenophon Papademetris
    Department of Biomedical Engineering, Yale University New Haven, CT 06520 8042, USA
    Inf Process Med Imaging 19:369-80. 2005
    ..Early validation results, using magnetic resonance spectroscopic imaging as a gold standard, are encouraging. We also present results demonstrating differences in fat distribution between male and female mice...
  26. ncbi request reprint Glutamatergic and GABAergic neurotransmitter cycling and energy metabolism in rat cerebral cortex during postnatal development
    Golam M I Chowdhury
    Department of Diagnostic Radiology, Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Cereb Blood Flow Metab 27:1895-907. 2007
    ..The findings show that glutamate and GABA neurons undergo large and approximately proportional increases in neurotransmitter cycling and oxidative energy metabolism during this major postnatal growth spurt...
  27. pmc High resolution NMR spectroscopy of rat brain in vivo through indirect zero-quantum-coherence detection
    Robin A de Graaf
    Departments of Diagnostic Radiology and Biomedical Engineering, Magnetic Resonance Research Center, Yale University School of Medicine, 300 Cedar Street, New Haven, CT 06520, USA
    J Magn Reson 187:320-6. 2007
    ..Unique spectral information which is normally not directly available from regular (1)H NMR spectra can be extracted and used for compound identification or improved prior knowledge during spectral fitting...
  28. pmc Natural abundance (17)O NMR spectroscopy of rat brain in vivo
    Robin A de Graaf
    Magnetic Resonance Research Center, Yale University, School of Medicine, New Haven, CT 06520 8043, USA
    J Magn Reson 193:63-7. 2008
    ..74 T. The chemical shifts and line widths of more than 20 oxygen-containing metabolites are established and the sensitivity and potential for (17)O-enriched NMR studies are estimated...
  29. ncbi request reprint Neuronal-glial glucose oxidation and glutamatergic-GABAergic function
    Fahmeed Hyder
    Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Cereb Blood Flow Metab 26:865-77. 2006
    ..The relationship observed between DeltaCMR(glc(ox), N) and DeltaV(cyc(tot)) is determined by glial glycolytic ATP as before. Quantitative aspects of the model, which can be tested by experimentation, are discussed...
  30. pmc Regional glucose metabolism and glutamatergic neurotransmission in rat brain in vivo
    Robin A de Graaf
    Magnetic Resonance Research Center and Departments of Diagnostic Radiology and Psychiatry, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 101:12700-5. 2004
    ..02 +/- 0.04, and 0.18 +/- 0.12 micromol/min per g, respectively. The exchange rate between the mitochondrial and cytosolic metabolite pools was fast relative to the neuronal tricarboxylic acid cycle flux for all cerebral tissue types...
  31. ncbi request reprint Energetic basis of brain activity: implications for neuroimaging
    Robert G Shulman
    Department of Diagnostic Radiology, Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, CT 06510, USA
    Trends Neurosci 27:489-95. 2004
    ..MRS and fMRI experiments show that the majority of cerebral activity, which is often disregarded in imaging experiments, is ongoing even when the brain appears to be doing nothing...
  32. pmc Astroglial contribution to brain energy metabolism in humans revealed by 13C nuclear magnetic resonance spectroscopy: elucidation of the dominant pathway for neurotransmitter glutamate repletion and measurement of astrocytic oxidative metabolism
    Vincent Lebon
    Howard Hughes Medical Institute, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Neurosci 22:1523-31. 2002
    ..The further application of this approach could potentially enlighten the role of astroglia in supporting brain glutamatergic activity and in neurological and psychiatric disease...
  33. pmc Cerebral energetics and spiking frequency: the neurophysiological basis of fMRI
    Arien J Smith
    Magnetic Resonance Research Center, Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 99:10765-70. 2002
    ..Because DeltaCMR(O2)/CMR(O2) had the same high spatial and temporal resolutions of the fMRI signal, these results show how BOLD imaging, when converted to DeltaCMR(O2)/CMR(O2), responds to localized changes in neuronal spike frequency...
  34. ncbi request reprint Detection of [1,6-13C2]-glucose metabolism in rat brain by in vivo 1H-[13C]-NMR spectroscopy
    Robin A de Graaf
    Magnetic Resonance Center, Department of Radiology, Yale University School of Medicine, New Haven, Connecticut 06520 8043, USA
    Magn Reson Med 49:37-46. 2003
    ..52 +/- 0.04 micromol/min/g, while the glutamatergic neurotransmitter flux equaled 0.25 +/- 0.05 micromol/min/g, in good correspondence with previously determined values...
  35. ncbi request reprint In vivo NMR studies of the glutamate neurotransmitter flux and neuroenergetics: implications for brain function
    Douglas L Rothman
    Magnetic Resonance Center for Research in Metabolism and Physiology, Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Annu Rev Physiol 65:401-27. 2003
    ..Here we review and discuss the importance of neurotransmission and neuroenergetics as measured by (13)C MRS for understanding brain function and interpreting fMRI...
  36. ncbi request reprint Adiabatic RARE imaging
    Robin A de Graaf
    Department of Diagnostic Radiology, Magnetic Resonance Center, Yale University School of Medicine, New Haven, Connecticut 06520 8043, USA
    NMR Biomed 16:29-35. 2003
    ..A two-scan RARE implementation provides sufficient spatial and temporal resolution for most applications. Quantitative mapping of T(1) relaxation and cerebral blood flow changes during forepaw stimulation in the rat are demonstrated...
  37. ncbi request reprint Quantitative 1H NMR spectroscopy of blood plasma metabolites
    Robin A de Graaf
    Magnetic Resonance Research Center, Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Anal Chem 75:2100-4. 2003
    ..The general nature of diffusion-sensitized NMR spectroscopy allows application on a wide range of biological fluids...
  38. ncbi request reprint In vivo 1H-[13C]-NMR spectroscopy of cerebral metabolism
    Robin A de Graaf
    Department of Diagnostic Radiology, Magnetic Resonance Research Center, Yale University School of Medicine, 300 Cedar Street, New Haven, CT 06520 8043, USA
    NMR Biomed 16:339-57. 2003
    ..Finally, (1)H-[(13)C]-NMR spectroscopy allows the detection of energy metabolism and neurotransmission during functional activation, thereby further strengthening our understanding of the neurochemical basis of brain function...
  39. ncbi request reprint Mapping cerebral glutamate 13C turnover and oxygen consumption by in vivo NMR
    Fahmeed Hyder
    Magnetic Resonance Research Center, Departments of Diagnostic Radiology, Biomedical Engineering, Yale University School of Medicine, New Haven, CT, USA
    Adv Exp Med Biol 530:29-39. 2003
    ..The regional variations in the basal awake state, which are expected to be even greater, should be considered to avoid partial-volume artifacts in functional activation studies of awake subjects...
  40. ncbi request reprint Determination of liposomal encapsulation efficiency using proton NMR spectroscopy
    Xian Man Zhang
    Department of Psychiatry, New Haven, CT 06520 8043, USA
    Chem Phys Lipids 127:113-20. 2004
    ....
  41. ncbi request reprint Coupling of glutamatergic neurotransmission and neuronal glucose oxidation over the entire range of cerebral cortex activity
    Anant B Patel
    Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Ann N Y Acad Sci 1003:452-3. 2003
  42. ncbi request reprint Family psychopathology and magnitude of reductions in occipital cortex GABA levels in panic disorder
    Andrew W Goddard
    Neuropsychopharmacology 29:639-40. 2004
  43. ncbi request reprint Expression of Drosophila trehalose-phosphate synthase in HEK-293 cells increases hypoxia tolerance
    Qiaofang Chen
    Departments of Pediatrics and Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:49113-8. 2003
    ..The mechanism of this protection is likely related to a decrease in protein denaturation, through protein-trehalose interactions, resulting in enhanced cellular recovery from hypoxic stress...
  44. ncbi request reprint Dominant events that modulate mass transfer coefficient of oxygen in cerebral cortex
    Fahmeed Hyder
    Magnetic Resonance Research Center, Departments of Diagnostic Radiology, Biomedical Engineering, Yale University School of Medicine, New Haven, CT, USA
    Adv Exp Med Biol 530:401-11. 2003
    ..unpacking of erythrocytes, or increase vs. decrease of dissolved oxygen in the tissue (i.e., processes which modify vessel-to-tissue oxygen tension) contribute(s) > 95% to changes in D...
  45. ncbi request reprint Differential glutamate dehydrogenase (GDH) activity profile in patients with temporal lobe epilepsy
    Gauri H Malthankar-Phatak
    Department of Pharmaceutical Sciences, College of Pharmacy, Idaho State University, Pocatello, Idaho 83209, USA
    Epilepsia 47:1292-9. 2006
    ....
  46. ncbi request reprint Chronic hypoxia in development selectively alters the activities of key enzymes of glucose oxidative metabolism in brain regions
    James C K Lai
    Department of Pharmaceutical Sciences, College of Pharmacy, Idaho State University, Pocatello, Idaho 83209 8334, USA
    Neurochem Res 28:933-40. 2003
    ..g., decreased in energy metabolism) in childhood diseases (e.g., sudden infant death syndrome) in which hypoxia plays a role...
  47. ncbi request reprint Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster
    Qiaofang Chen
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 277:3274-9. 2002
    ..We conclude that trehalose contributes to anoxia tolerance in flies; this protection is likely to be due to a reduction of protein aggregation...

Research Grants13