Mei Chu Lo
Affiliation: Wyeth Research
- Evaluation of fluorescence-based thermal shift assays for hit identification in drug discoveryMei Chu Lo
Biophysics Enzymology Chemical and Screening Sciences, Wyeth Research, Pearl River, NY 10965, USA
Anal Biochem 332:153-9. 2004..The potential pitfalls in the data analysis of thermal shift assays are also discussed...
- A highly sensitive high-throughput luminescence assay for malonyl-CoA decarboxylaseMei Chu Lo
Chemistry Research and Discovery, Amgen, South San Francisco, CA 94080, USA
Anal Biochem 376:122-30. 2008..This assay is also amenable to automation for high-throughput screening and yields excellent assay statistics (Z' > 0.8). In addition, it can be applied to the screening for inhibitors of any other enzymes that generate acetyl-CoA...
- Probing the interaction of HTI-286 with tubulin using a stilbene analogueMei Chu Lo
Biophysics Enzymology Chemical and Screening Sciences, Wyeth Research, 401 North Middletown Road, Pearl River, New York 10965, USA
J Am Chem Soc 126:9898-9. 2004..The stable complex between the inhibitor and tubulin monomer was not detected under our experimental conditions. The binding site of the stilbene analogue does not overlap with the classic tubulin-binding agent, colchicine...
- Biophysical characterization of the interactions of HTI-286 with tubulin heterodimer and microtubulesGirija Krishnamurthy
Biophysics Enzymology, Screening Sciences, Oncology Research, and Chemical Sciences, Wyeth Research, Pearl River, New York 10965, USA
Biochemistry 42:13484-95. 2003..In contrast to the microtubule-stabilizing effects of paclitaxel, both HTI-286 and hemiasterlin depolymerize preassembled microtubules at micromolar concentrations...
- Discovery and initial optimization of 5,5'-disubstituted aminohydantoins as potent beta-secretase (BACE1) inhibitorsPawel Nowak
Chemical Sciences, Wyeth Research, 401 N Middletown Road, Pearl River, NY 10965, United States
Bioorg Med Chem Lett 20:632-5. 2010..Lead optimization using structure-based focused libraries led to the identification of low nanomolar BACE1 inhibitors such as 20b with substituents which extend from the S(1) to the S(3) pocket...
- Identification, characterization, and crystal structure of Bacillus subtilis nicotinic acid mononucleotide adenylyltransferaseAndrea M Olland
Wyeth Ayerst Research, Department of Biological Chemistry, Cambridge, Massachusetts 02140, USA
J Biol Chem 277:3698-707. 2002..Interactions of the nicotinic acid with backbone atoms indicate the structural basis for specificity...