Steven A Haney
Affiliation: Wyeth Research
- RNAi and high-content screening in target identification and validationSteven A Haney
Wyeth Research, Section of Applied Genomics, Department of Biological Technologies, Cambridge, MA 02140, USA
IDrugs 8:997-1001. 2005..However, combining RNAi and HCS provides significant and unique advantages to a target validation program...
- Increasing the robustness and validity of RNAi screensSteven A Haney
Department of Biological Technologies, Wyeth Research, 35 Cambridge Park Drive, Cambridge, MA 02140, USA
Pharmacogenomics 8:1037-49. 2007..This review will discuss how these approaches can improve RNAi screening data at the community level and for an individual researcher trying to manage an RNAi screen...
- Anticancer drug development incorporating high-content screening and RNAi: synergistic approaches to improve target identification and validationSteven A Haney
Wyeth Research, Department of Biological Technologies, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
Expert Opin Drug Discov 1:19-29. 2006..This review describes RNAi and HCS technologies as they apply to drug target validation, and discusses efforts to integrate them. Particular focus is applied to aspects of HCS that mitigate some of the challenges inherent to RNAi...
- Transcriptional changes associated with breast cancer occur as normal human mammary epithelial cells overcome senescence barriers and become immortalizedYizheng Li
Section of Bioinformatics, Department of Biological Technologies, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
Mol Cancer 6:7. 2007..HMEC that have overcome the second senescence barrier are immortalized...
- Impact of image segmentation on high-content screening data quality for SK-BR-3 cellsAndrew A Hill
Department of Biological Technologies, Wyeth Research, 35 CambridgePark Drive, Cambridge, MA 02140, USA
BMC Bioinformatics 8:340. 2007..We asked if segmentation errors were common for a clinically relevant cell line, if such errors had measurable effects on the data, and if HCS data could be improved by automated identification of well-segmented cells...
- High-content screening moves to the front of the lineSteven A Haney
Department of Biological Technologies, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
Drug Discov Today 11:889-94. 2006..The use of HCS in target validation is expanding the work that can be done at this stage, especially the range of targets that can be characterized, and putting it into a more biological context...
- Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coliSteven A Haney
Department of Infectious Disease, Wyeth Research, 401 N, Middletown Rd, Pearl River, NY 10965, USA
BMC Genomics 4:36. 2003..coli or yeast gene products in these systems has been shown to be growth inhibitory, and therefore these clones are underrepresented (or completely lost) in the amplified library...
- Expanding the repertoire of RNA interference screens for developing new anticancer drug targetsSteven A Haney
Wyeth Research, Department of Biological Technologies, 35 Cambridge Park Drive, Cambridge, MA 02140, USA
Expert Opin Ther Targets 11:1429-41. 2007..RNAi screening can enable critical evaluations of both the roles of these concepts in tumor development and provide starting points for new therapeutic programs targeting emerging areas of cancer cell biology...
- Single cell cytometry of protein function in RNAi treated cells and in native populationsPeter LaPan
Department of Biological Technologies, Oncology Research, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
BMC Cell Biol 9:43. 2008..Such approaches are important for immune cells analyzed by flow cytometry, but have not been broadly available for adherent cells that are critical to the study of solid-tumor cancers and other disease models...
- Genomics in anti-infective drug discovery--getting to endgameSteven A Haney
Wyeth Ayerst Research, 401 N Middletown Road, Pearl River, NY 10965, USA
Curr Pharm Des 8:1099-118. 2002....
- Combinatorial synthesis of substituted 3-(2-indolyl)piperidines and 2-phenyl indoles as inhibitors of ZipA-FtsZ interactionLee D Jennings
Wyeth Research, Department of Medicinal Chemistry, 401 N Middletown Road, Pearl River, NY 10965, USA
Bioorg Med Chem 12:5115-31. 2004..Compounds were demonstrated to bind to the FtsZ binding domain of ZipA by HSQC NMR and to inhibit cell division in a cell elongation assay...