- Detection and quantification of mRNA in single human polar bodies: a minimally invasive test of gene expression during oogenesisPeter C Klatsky
Division of Reproductive Endocrinology and Infertility, Women and Infants Hospital, Alpert School of Medicine, Brown University, 101 Dudley Street, Providence, RI 02905, USA
Mol Hum Reprod 16:938-43. 2010..Pre-amplification of cDNA synthesized without RNA isolation can facilitate the quantitative detection of mRNA in single human polar bodies...
- Use of sea stars to study basic reproductive processesGary M Wessel
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
Syst Biol Reprod Med 56:236-45. 2010..This review will highlight the fundamental protocols to enable a new investigator to perform an array of approaches on this organism, including oocyte isolation, microinjection, and even single cell quantitative PCR...