NYLES CHARON

Summary

Affiliation: West Virginia University
Country: USA

Publications

  1. pmc Genetic analysis of spirochete flagellin proteins and their involvement in motility, filament assembly, and flagellar morphology
    Chunhao Li
    Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Bacteriol 190:5607-15. 2008
  2. pmc The unique paradigm of spirochete motility and chemotaxis
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Health Sciences Center, Morgantown, 26506 9177, USA
    Annu Rev Microbiol 66:349-70. 2012
  3. ncbi request reprint Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, West Virginia University, Box 9177, Morgantown, West Virginia 26506 9177, USA
    Annu Rev Genet 36:47-73. 2002
  4. pmc The decrease in FlaA observed in a flaB mutant of Borrelia burgdorferi occurs posttranscriptionally
    M A Motaleb
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Box 9177, Robert C Byrd Health Sciences Center, Morgantown, WV 26506 9177, USA
    J Bacteriol 186:3703-11. 2004
  5. pmc The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology
    Nyles W Charon
    Department of Microbiology, Immunology and Cell Biology, Robert C Byrd Health Sciences Center, West Virginia University, Morgantown, West Virginia 26506 9177, USA
    J Bacteriol 191:600-7. 2009
  6. pmc Borrelia burgdorferi uniquely regulates its motility genes and has an intricate flagellar hook-basal body structure
    Melanie S Sal
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Robert C Byrd Health Sciences Center, Morgantown, WV 26506 9177, USA
    J Bacteriol 190:1912-21. 2008
  7. pmc Asymmetrical flagellar rotation in Borrelia burgdorferi nonchemotactic mutants
    Chunhao Li
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, Box 9177, West Virginia University, Morgantown, WV 26506 9177, USA
    Proc Natl Acad Sci U S A 99:6169-74. 2002
  8. pmc Mycoplasma takes a walk
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, Robert C Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA
    Proc Natl Acad Sci U S A 102:13713-4. 2005
  9. ncbi request reprint Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi
    MD A MOTALEB
    Department of Microbiology, West Virginia University, Morgantown, West Virginia, USA
    Methods Enzymol 422:438-47. 2007
  10. ncbi request reprint Isolation and characterization of chemotaxis mutants of the Lyme disease Spirochete Borrelia burgdorferi using allelic exchange mutagenesis, flow cytometry, and cell tracking
    MD A MOTALEB
    Department of Microbiology, West Virginia University, Morgantown, West Virginia, USA
    Methods Enzymol 422:421-37. 2007

Research Grants

  1. ANALYSIS OF BORRELIA BURGDORFERI MOTILITY
    NYLES CHARON; Fiscal Year: 1993
  2. ANALYSIS OF BORRELIA BURGDORFERI MOTILITY
    NYLES CHARON; Fiscal Year: 1992
  3. An analysis of Borrelia burgdorferi motility
    NYLES CHARON; Fiscal Year: 2007

Collaborators

Detail Information

Publications16

  1. pmc Genetic analysis of spirochete flagellin proteins and their involvement in motility, filament assembly, and flagellar morphology
    Chunhao Li
    Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Bacteriol 190:5607-15. 2008
    ..Finally, linear elasticity theory indicates that flagellar stiffness directly affects the spirochete's swimming speed...
  2. pmc The unique paradigm of spirochete motility and chemotaxis
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Health Sciences Center, Morgantown, 26506 9177, USA
    Annu Rev Microbiol 66:349-70. 2012
    ..The results indicate that the motility paradigms of flagellated bacteria do not apply to these unique bacteria...
  3. ncbi request reprint Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, West Virginia University, Box 9177, Morgantown, West Virginia 26506 9177, USA
    Annu Rev Genet 36:47-73. 2002
    ..Motility is likely to play a major role in the disease process. This review summarizes the progress in the genetics of motility and chemotaxis of spirochetes, and points to new directions for future experimentation...
  4. pmc The decrease in FlaA observed in a flaB mutant of Borrelia burgdorferi occurs posttranscriptionally
    M A Motaleb
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Box 9177, Robert C Byrd Health Sciences Center, Morgantown, WV 26506 9177, USA
    J Bacteriol 186:3703-11. 2004
    ..Our results indicate that the lack of FlaB negatively influences the amount of FlaA found in the cell and that this effect is at the level of either translational control or protein turnover...
  5. pmc The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology
    Nyles W Charon
    Department of Microbiology, Immunology and Cell Biology, Robert C Byrd Health Sciences Center, West Virginia University, Morgantown, West Virginia 26506 9177, USA
    J Bacteriol 191:600-7. 2009
    ....
  6. pmc Borrelia burgdorferi uniquely regulates its motility genes and has an intricate flagellar hook-basal body structure
    Melanie S Sal
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Robert C Byrd Health Sciences Center, Morgantown, WV 26506 9177, USA
    J Bacteriol 190:1912-21. 2008
    ..In addition, we also present evidence that the hook protein in B. burgdorferi forms a high-molecular-weight complex and that formation of this complex occurs in the periplasmic space...
  7. pmc Asymmetrical flagellar rotation in Borrelia burgdorferi nonchemotactic mutants
    Chunhao Li
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, Box 9177, West Virginia University, Morgantown, WV 26506 9177, USA
    Proc Natl Acad Sci U S A 99:6169-74. 2002
    ..We propose that certain factors within the spirochete localize at the flagellar motors at one end of the cell to effect this asymmetry...
  8. pmc Mycoplasma takes a walk
    Nyles W Charon
    Department of Microbiology, Immunology, and Cell Biology, Robert C Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA
    Proc Natl Acad Sci U S A 102:13713-4. 2005
  9. ncbi request reprint Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi
    MD A MOTALEB
    Department of Microbiology, West Virginia University, Morgantown, West Virginia, USA
    Methods Enzymol 422:438-47. 2007
    ..This chapter describes protocols for assessing B. burgdorferi CheA autophosphorylation, transfer of phosphate from CheA-P to CheY, and CheY-P dephosphorylation...
  10. ncbi request reprint Isolation and characterization of chemotaxis mutants of the Lyme disease Spirochete Borrelia burgdorferi using allelic exchange mutagenesis, flow cytometry, and cell tracking
    MD A MOTALEB
    Department of Microbiology, West Virginia University, Morgantown, West Virginia, USA
    Methods Enzymol 422:421-37. 2007
    ..It should be possible to adapt these procedures to study other spirochete species, as well as other species of bacteria, especially those that have long generation times...
  11. pmc CheX is a phosphorylated CheY phosphatase essential for Borrelia burgdorferi chemotaxis
    M A Motaleb
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, West Virginia University, Morgantown, 26506 9177, USA
    J Bacteriol 187:7963-9. 2005
    ..burgdorferi CheX is a CheY-P phosphatase that is essential for chemotaxis and motility, which is consistent with CheX being the only CheY-P phosphatase in the B. burgdorferi chemotaxis signal transduction pathway...
  12. pmc Identification of specific chemoattractants and genetic complementation of a Borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay
    Richard G Bakker
    Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 9177, USA
    Appl Environ Microbiol 73:1180-8. 2007
    ..burgdorferi and the use of flow cytometry for spirochete enumeration. The method should also be useful for assaying chemotaxis for other slow-growing prokaryotic species and in specific environments in nature...
  13. pmc Spent culture medium from virulent Borrelia burgdorferi increases permeability of individually perfused microvessels of rat mesentery
    Xueping Zhou
    Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV, USA
    PLoS ONE 3:e4101. 2008
    ..The objective of the present study was to investigate if Bb produces a mediator(s) that can directly activate endothelial cells resulting in increases in permeability in intact microvessels in the absence of blood cells...
  14. ncbi request reprint The flagellar cytoskeleton of the spirochetes
    Charles W Wolgemuth
    Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030 3505, USA
    J Mol Microbiol Biotechnol 11:221-7. 2006
    ..Rotation of the flagella in the above species by a flagellar motor induces changes in the cell morphology that drives motility. Mutants that do not produce flagella have a markedly different shape than wild-type cells...
  15. doi request reprint Electron cryotomography reveals novel structures of a recently cultured termite gut spirochete
    Charles Wolgemuth
    Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030 3505, USA
    Mol Microbiol 67:1181-3. 2008
    ..It also illustrates that newly cultured recalcitrant bacteria from complex environments are likely to possess novel structures not previously seen in other species...
  16. pmc Bright lights, abundant operons--fluorescence and genomic technologies advance studies of bacterial locomotion and signal transduction: review of the BLAST meeting, Cuernavaca, Mexico, 14 to 19 January 2001
    Robert B Bourret
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599 7290, USA
    J Bacteriol 184:1-17. 2002

Research Grants14

  1. ANALYSIS OF BORRELIA BURGDORFERI MOTILITY
    NYLES CHARON; Fiscal Year: 1993
    ..The results obtained should enable models and conclusions be drawn concerning mechanisms of B. burgdorferi motility. ..
  2. ANALYSIS OF BORRELIA BURGDORFERI MOTILITY
    NYLES CHARON; Fiscal Year: 1992
    ..The results obtained should enable models and conclusions be drawn concerning mechanisms of B. burgdorferi motility...
  3. An analysis of Borrelia burgdorferi motility
    NYLES CHARON; Fiscal Year: 2007
    ..The experiments proposed should yield information with respect to the roles of chemotaxis and motility of B. burgdorferi in Lyme disease, and perhaps lay the foundation for new methods for prevention and treatment. ..