Youming Xie

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. pmc Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan, United States of America
    PLoS ONE 5:e9877. 2010
  2. doi request reprint Structure, assembly and homeostatic regulation of the 26S proteasome
    Youming Xie
    Barbara Ann Karmanos Cancer Institute, Department of Oncology, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    J Mol Cell Biol 2:308-17. 2010
  3. doi request reprint Feedback regulation of proteasome gene expression and its implications in cancer therapy
    Youming Xie
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    Cancer Metastasis Rev 29:687-93. 2010
  4. pmc Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    Mol Cell Biol 28:1404-12. 2008
  5. ncbi request reprint Identification of the preferential ubiquitination site and ubiquitin-dependent degradation signal of Rpn4
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 281:10657-62. 2006
  6. ncbi request reprint Ubiquitin-mediated degradation of Rpn4 is controlled by a phosphorylation-dependent ubiquitylation signal
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Avenue, Detroit, MI 48201, USA
    Biochim Biophys Acta 1773:1672-80. 2007
  7. pmc Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions
    Xiaogang Wang
    Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Genetics 184:335-42. 2010
  8. ncbi request reprint Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase
    Li Wang
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Avenue, Detroit, MI 48201, USA
    J Biol Chem 279:55218-23. 2004
  9. ncbi request reprint A synthetic defect in protein degradation caused by loss of Ufd4 and Rad23
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Ave, Detroit, MI 48201, USA
    Biochem Biophys Res Commun 341:648-52. 2006
  10. doi request reprint The transcription activation domain of Rpn4 is separate from its degrons
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Avenue, Detroit, MI 48201, USA
    Int J Biochem Cell Biol 42:282-6. 2010

Collaborators

Detail Information

Publications21

  1. pmc Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan, United States of America
    PLoS ONE 5:e9877. 2010
    ..We have demonstrated that inhibition of Rpn4 degradation sensitizes cells to DNA damage, particularly in response to high doses of DNA damaging agents. The underlying mechanism, however, remains unclear...
  2. doi request reprint Structure, assembly and homeostatic regulation of the 26S proteasome
    Youming Xie
    Barbara Ann Karmanos Cancer Institute, Department of Oncology, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    J Mol Cell Biol 2:308-17. 2010
    ..The implications of these new developments in cancer therapy will also be discussed...
  3. doi request reprint Feedback regulation of proteasome gene expression and its implications in cancer therapy
    Youming Xie
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    Cancer Metastasis Rev 29:687-93. 2010
    ..This finding also has important implications in cancer therapy that uses small molecule inhibitors to target the proteasome...
  4. pmc Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Ave, Detroit, MI 48201, USA
    Mol Cell Biol 28:1404-12. 2008
    ..Together, these data suggest that Mub1 and Ubr2 cooperate to transfer ubiquitin to Rpn4 from Rad6 and that Mub1 may switch from a partner to a substrate of the Ubr2/Rad6 ubiquitin ligase...
  5. ncbi request reprint Identification of the preferential ubiquitination site and ubiquitin-dependent degradation signal of Rpn4
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 281:10657-62. 2006
    ..We further demonstrated that lysine 187 and a proximal acidic domain constitute a portable degradation signal. The implications of our data are discussed...
  6. ncbi request reprint Ubiquitin-mediated degradation of Rpn4 is controlled by a phosphorylation-dependent ubiquitylation signal
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Avenue, Detroit, MI 48201, USA
    Biochim Biophys Acta 1773:1672-80. 2007
    ..This study also suggests that binding to E3 may be only a part of the function of a ubiquitylation signal...
  7. pmc Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions
    Xiaogang Wang
    Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Genetics 184:335-42. 2010
    ..Rpn4 thus represents an important stress-responsive mediator whose degradation as well as availability are critical for cell survival under stressed conditions...
  8. ncbi request reprint Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase
    Li Wang
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Avenue, Detroit, MI 48201, USA
    J Biol Chem 279:55218-23. 2004
    ..This study not only identified the ubiquitination apparatus for Rpn4 but also unveiled the first physiological substrate of Ubr2. The biological significance of Ubr2-mediated degradation of Rpn4 is also discussed...
  9. ncbi request reprint A synthetic defect in protein degradation caused by loss of Ufd4 and Rad23
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Ave, Detroit, MI 48201, USA
    Biochem Biophys Res Commun 341:648-52. 2006
    ..The current work also provides a direction for further investigation of the physiological functions of the UFD pathway...
  10. doi request reprint The transcription activation domain of Rpn4 is separate from its degrons
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Avenue, Detroit, MI 48201, USA
    Int J Biochem Cell Biol 42:282-6. 2010
    ..This study provides important information for further understanding the biological functions of Rpn4 and the proteasome system...
  11. pmc The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits
    Seung Wook Ha
    Barbara Ann Karmanos Cancer Institute and Department of Oncology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Biochem Biophys Res Commun 419:226-31. 2012
    ..This is the first time that specific 19S RP subunits have been identified interacting with a Ub-independent degron. This study provides insight into the mechanism by which Ub-independent substrates are recruited to the 26S proteasome...
  12. doi request reprint The CCAAT box-binding transcription factor NF-Y regulates basal expression of human proteasome genes
    Haiming Xu
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Biochim Biophys Acta 1823:818-25. 2012
    ..This study unveils a new role for NF-Y in the regulation of human proteasome genes and suggests that NF-Y may be a potential target for cancer therapy...
  13. pmc Nuclear import factor Srp1 and its associated protein Sts1 couple ribosome-bound nascent polypeptides to proteasomes for cotranslational degradation
    Seung Wook Ha
    From the Karmanos Cancer Institute, Department of Oncology, and Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201
    J Biol Chem 289:2701-10. 2014
    ..This study unveils a previously unknown role for Srp1 and Sts1 in cotranslational protein degradation and suggests a novel model whereby Srp1 and Sts1 cooperate to couple proteasomes to ribosome-bound nascent polypeptides. ..
  14. ncbi request reprint Homeostatic regulation of the proteasome via an Rpn4-dependent feedback circuit
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, 110 Warren Avenue, Detroit, MI 48201, USA
    Biochem Biophys Res Commun 321:51-7. 2004
    ..This study provides important insights into the mechanism underlying proteasome homeostasis...
  15. ncbi request reprint Diminished feedback regulation of proteasome expression and resistance to proteasome inhibitors in breast cancer cells
    Haiming Xu
    Department of Pathology, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Breast Cancer Res Treat 107:267-74. 2008
    ..The likelihood of combination therapy with Bortezomib and other anti-cancer agents for breast cancer is also discussed...
  16. ncbi request reprint The armadillo repeats of the Ufd4 ubiquitin ligase recognize ubiquitin-fusion proteins
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, 110 E Warren Avenue, Detroit, MI 48201, USA
    FEBS Lett 581:265-70. 2007
    ..Disruption of the ARM repeats abolishes the ubiquitylating activity of Ufd4. This study uncovers a new role of the ARM repeats in protein ubiquitylation...
  17. ncbi request reprint Proteasomal degradation of RPN4 via two distinct mechanisms, ubiquitin-dependent and -independent
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State, University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 279:23851-4. 2004
    ..cerevisiae that can be degraded through ubiquitylation or without prior ubiquitylation. This finding makes it possible to use both yeast genetics and biochemical analysis to investigate the mechanism of ubiquitin-independent proteolysis...
  18. pmc Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases
    Ying Wang
    Barbara Ann Karmanos Cancer Institute and Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA
    Oncotarget 5:2044-51. 2014
    ..This study uncovers a previously unknown functional link between gp78 and TRIM25 and provides mechanistic insight into gp78-mediated protein ubiquitylation. ..
  19. pmc Dyclonine and alverine citrate enhance the cytotoxic effects of proteasome inhibitor MG132 on breast cancer cells
    Donghong Ju
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Int J Mol Med 23:205-9. 2009
    ..This study also highlights an important yeast genetic approach to identification of potential therapeutics that can be used for combination therapy with proteasome inhibitors...
  20. pmc Autocrine motility factor modulates EGF-mediated invasion signaling
    Dhong Hyo Kho
    Authors Affiliations Departments of Oncology and Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan
    Cancer Res 74:2229-37. 2014
    ..Taken together, our findings show how AMF modulates EGF-induced invasion while affecting acquired resistance to cytotoxic drugs in the tumor microenvironment...
  21. pmc Pharmacologic ER stress induces non-alcoholic steatohepatitis in an animal model
    Jin Sook Lee
    Center for Molecular Medicine and Genetics, the Wayne State University School of Medicine, Detroit, MI 48201, USA
    Toxicol Lett 211:29-38. 2012
    ..Our study not only confirmed that pharmacologic ER stress is a strong "hit" that triggers NASH, but also demonstrated crucial molecular links between ER stress, lipid metabolism, and inflammation in the liver in vivo...