ELLEN TISDALE

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. ncbi Rab2 purification and interaction with protein kinase C iota/lambda and glyceraldehyde-3-phosphate dehydrogenase
    Ellen J Tisdale
    Methods Enzymol 403:381-91. 2005
  2. ncbi Rab2 protein enhances coatomer recruitment to pre-Golgi intermediates
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 273:17269-77. 1998
  3. ncbi A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, 540 East Canfield Avenue, 6374 Scott Hall, Detroit, MI 48201, USA
    Traffic 8:733-41. 2007
  4. ncbi Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to Golgi transport exclusive of its glycolytic activity
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 279:54046-52. 2004
  5. ncbi Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation
    Ellen J Tisdale
    Department of Pharmacology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 278:52524-30. 2003
  6. ncbi Rab2 utilizes glyceraldehyde-3-phosphate dehydrogenase and protein kinase C{iota} to associate with microtubules and to recruit dynein
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 284:5876-84. 2009
  7. ncbi Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 278:38015-21. 2003
  8. ncbi Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 277:3334-41. 2002
  9. ncbi Rab2 requires PKC iota/lambda to recruit beta-COP for vesicle formation
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, 540 E Canfield Ave, Detroit, MI 48201, USA
    Traffic 1:702-12. 2000
  10. ncbi Glyceraldehyde-3-phosphate dehydrogenase is required for vesicular transport in the early secretory pathway
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 276:2480-6. 2001

Research Grants

  1. VTC Events Mediated by the Rab2 Complex
    Ellen J Tisdale; Fiscal Year: 2010

Detail Information

Publications13

  1. ncbi Rab2 purification and interaction with protein kinase C iota/lambda and glyceraldehyde-3-phosphate dehydrogenase
    Ellen J Tisdale
    Methods Enzymol 403:381-91. 2005
    ..Additionally, in vivo and in vitro assays are outlined that are employed to demonstrate Rab2 interaction with the downstream effectors aPKCiota/lambda and GAPDH...
  2. ncbi Rab2 protein enhances coatomer recruitment to pre-Golgi intermediates
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 273:17269-77. 1998
    ..Our results suggest that the Rab2 peptide causes the persistent recruitment of COPI to pre-Golgi intermediates which ultimately arrests protein transport due to the inability of membranes to uncoat...
  3. ncbi A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, 540 East Canfield Avenue, 6374 Scott Hall, Detroit, MI 48201, USA
    Traffic 8:733-41. 2007
    ..Our results suggest that Src plays a pivotal role in regulating the interaction of Rab2 effectors on the VTC...
  4. ncbi Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to Golgi transport exclusive of its glycolytic activity
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 279:54046-52. 2004
    ..These data suggest that GAPDH imparts a unique function necessary for membrane trafficking from VTCs that does not require GAPDH glycolytic activity...
  5. ncbi Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation
    Ellen J Tisdale
    Department of Pharmacology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 278:52524-30. 2003
    ..These results suggest that a downstream effector recruited to the VTC stimulates PKCiota/lambda-mediated GAPDH phosphorylation by alleviating the inhibition imposed by Rab2-PKCiota/lambda interaction...
  6. ncbi Rab2 utilizes glyceraldehyde-3-phosphate dehydrogenase and protein kinase C{iota} to associate with microtubules and to recruit dynein
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 284:5876-84. 2009
    ..These combined results suggest a mechanism by which Rab2 controls MT and motor recruitment to vesicular tubular clusters...
  7. ncbi Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 278:38015-21. 2003
    ....
  8. ncbi Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 277:3334-41. 2002
    ..These combined results suggest that Rab2 stimulated PKCiota/lambda and GAPDH recruitment to VTCs, and the subsequent PKCiota/lambda phosphorylation of GAPDH ultimately influences MT dynamics in the early secretory pathway...
  9. ncbi Rab2 requires PKC iota/lambda to recruit beta-COP for vesicle formation
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, 540 E Canfield Ave, Detroit, MI 48201, USA
    Traffic 1:702-12. 2000
    ..These data suggest that Rab2 causes translocation of PKC iota/lambda to vesicular tubular clusters (VTCs), which promotes the recruitment of COPI to generate retrograde-transport vesicles...
  10. ncbi Glyceraldehyde-3-phosphate dehydrogenase is required for vesicular transport in the early secretory pathway
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 276:2480-6. 2001
    ..These data suggest that GAPDH is required for ER to Golgi transport. We propose that membranes incubated with anti-GAPDH and Rab2 form "dead end" vesicles that are unable to transport and fuse with the acceptor compartment...
  11. ncbi A Rab2 mutant with impaired GTPase activity stimulates vesicle formation from pre-Golgi intermediates
    E J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Mol Biol Cell 10:1837-49. 1999
    ..These data suggest that the Rab2 protein plays a role in the low-temperature-sensitive step that regulates membrane flow from VTCs to the Golgi complex and back to the ER...
  12. ncbi Src-dependent aprotein kinase C iota/lambda (aPKCiota/lambda) tyrosine phosphorylation is required for aPKCiota/lambda association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates
    Ellen J Tisdale
    Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 281:8436-42. 2006
    ....
  13. ncbi The N'-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan Plasmodium falciparum mediates GTPase Rab2-dependent recruitment to membranes
    Claudia A Daubenberger
    Molecular Immunology, Swiss Tropical Institute, Socinstr 57, CH 4002 Basel, Switzerland
    Biol Chem 384:1227-37. 2003
    ..Together, these results suggest that pfGAPDH exerts non-glycolytic function(s) in P. falciparum, possibly including a role in vesicular transport and biogenesis of apical organelles...

Research Grants5

  1. VTC Events Mediated by the Rab2 Complex
    Ellen J Tisdale; Fiscal Year: 2010
    ..The studies proposed in this application will develop a new and novel paradigm that couples Rab2-Apkc lA-GAPDH dysfunction with pathological consequences. ..