ELLEN TISDALE

Summary

Affiliation: Wayne State University
Country: USA

Publications

  1. request reprint
    Tisdale E, Jackson M. Rab2 protein enhances coatomer recruitment to pre-Golgi intermediates. J Biol Chem. 1998;273:17269-77 pubmed
    ..Our results suggest that the Rab2 peptide causes the persistent recruitment of COPI to pre-Golgi intermediates which ultimately arrests protein transport due to the inability of membranes to uncoat. ..
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    Tisdale E. Glyceraldehyde-3-phosphate dehydrogenase is required for vesicular transport in the early secretory pathway. J Biol Chem. 2001;276:2480-6 pubmed
    ..These data suggest that GAPDH is required for ER to Golgi transport. We propose that membranes incubated with anti-GAPDH and Rab2 form "dead end" vesicles that are unable to transport and fuse with the acceptor compartment. ..
  3. request reprint
    Tisdale E. Rab2 requires PKC iota/lambda to recruit beta-COP for vesicle formation. Traffic. 2000;1:702-12 pubmed
    ..These data suggest that Rab2 causes translocation of PKC iota/lambda to vesicular tubular clusters (VTCs), which promotes the recruitment of COPI to generate retrograde-transport vesicles. ..
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    Tisdale E. Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway. J Biol Chem. 2002;277:3334-41 pubmed
    ..These combined results suggest that Rab2 stimulated PKCiota/lambda and GAPDH recruitment to VTCs, and the subsequent PKCiota/lambda phosphorylation of GAPDH ultimately influences MT dynamics in the early secretory pathway. ..
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    Tisdale E, Wang J, Silver R, Artalejo C. Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates. J Biol Chem. 2003;278:38015-21 pubmed
  6. Tisdale E. A Rab2 mutant with impaired GTPase activity stimulates vesicle formation from pre-Golgi intermediates. Mol Biol Cell. 1999;10:1837-49 pubmed
    ..These data suggest that the Rab2 protein plays a role in the low-temperature-sensitive step that regulates membrane flow from VTCs to the Golgi complex and back to the ER. ..
  7. Tisdale E, Azizi F, Artalejo C. Rab2 utilizes glyceraldehyde-3-phosphate dehydrogenase and protein kinase C{iota} to associate with microtubules and to recruit dynein. J Biol Chem. 2009;284:5876-84 pubmed publisher
    ..These combined results suggest a mechanism by which Rab2 controls MT and motor recruitment to vesicular tubular clusters. ..
  8. Tisdale E, Talati N, Artalejo C, Shisheva A. GAPDH binds Akt to facilitate cargo transport in the early secretory pathway. Exp Cell Res. 2016;349:310-319 pubmed publisher
    ..We propose a mechanism whereby the association of GAPDH with Akt or with aPKC serves as a switch to discriminate between anterograde directed cargo and recycling cargo retrieved back to the ER, respectively. ..
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    Tisdale E. Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation. J Biol Chem. 2003;278:52524-30 pubmed
    ..These results suggest that a downstream effector recruited to the VTC stimulates PKCiota/lambda-mediated GAPDH phosphorylation by alleviating the inhibition imposed by Rab2-PKCiota/lambda interaction. ..

More Information

Publications11

  1. request reprint
    Tisdale E, Kelly C, Artalejo C. Glyceraldehyde-3-phosphate dehydrogenase interacts with Rab2 and plays an essential role in endoplasmic reticulum to Golgi transport exclusive of its glycolytic activity. J Biol Chem. 2004;279:54046-52 pubmed
    ..These data suggest that GAPDH imparts a unique function necessary for membrane trafficking from VTCs that does not require GAPDH glycolytic activity. ..
  2. Tisdale E, Artalejo C. A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events. Traffic. 2007;8:733-41 pubmed
    ..Our results suggest that Src plays a pivotal role in regulating the interaction of Rab2 effectors on the VTC. ..